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Protein

Uroplakin-1a

Gene

UPK1A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the asymmetric unit membrane (AUM); a highly specialized biomembrane elaborated by terminally differentiated urothelial cells. May play an important role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Uroplakin-1a
Short name:
UP1a
Alternative name(s):
Tetraspanin-21
Short name:
Tspan-21
Uroplakin Ia
Short name:
UPIa
Short name:
UPKa
Gene namesi
Name:UPK1A
Synonyms:TSPAN21
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:12577. UPK1A.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414CytoplasmicSequence analysisAdd
BLAST
Transmembranei15 – 3521HelicalSequence analysisAdd
BLAST
Topological domaini36 – 5924ExtracellularSequence analysisAdd
BLAST
Transmembranei60 – 8627HelicalSequence analysisAdd
BLAST
Topological domaini87 – 915CytoplasmicSequence analysis
Transmembranei92 – 11221HelicalSequence analysisAdd
BLAST
Topological domaini113 – 230118ExtracellularSequence analysisAdd
BLAST
Transmembranei231 – 25222HelicalSequence analysisAdd
BLAST
Topological domaini253 – 2586CytoplasmicSequence analysis

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA37209.

Polymorphism and mutation databases

BioMutaiUPK1A.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 258258Uroplakin-1aPRO_0000219286Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO00322.
PRIDEiO00322.

Expressioni

Tissue specificityi

High expression restricted to ureteric urothelium (most superficial cells); low expression in prostate. Expression in normal urothelial cells is lost in culture. Some expression in tumor cell lines derived from urothelial malignancies.2 Publications

Gene expression databases

BgeeiO00322.
CleanExiHS_UPK1A.
GenevisibleiO00322. HS.

Organism-specific databases

HPAiHPA049879.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Interacts with uroplakin-2 (UPK2) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi116233. 80 interactions.
STRINGi9606.ENSP00000222275.

Structurei

3D structure databases

ProteinModelPortaliO00322.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3882. Eukaryota.
ENOG4111IRY. LUCA.
GeneTreeiENSGT00390000000126.
HOGENOMiHOG000230653.
HOVERGENiHBG054305.
InParanoidiO00322.
KOiK17347.
OMAiHVDYLFT.
OrthoDBiEOG76HQ28.
PhylomeDBiO00322.
TreeFamiTF335659.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00322-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASAAAAEAE KGSPVVVGLL VVGNIIILLS GLSLFAETIW VTADQYRVYP
60 70 80 90 100
LMGVSGKDDV FAGAWIAIFC GFSFFMVASF GVGAALCRRR SMVLTYLVLM
110 120 130 140 150
LIVYIFECAS CITSYTHRDY MVSNPSLITK QMLTFYSADT DQGQELTRLW
160 170 180 190 200
DRVMIEQECC GTSGPMDWVN FTSAFRAATP EVVFPWPPLC CRRTGNFIPL
210 220 230 240 250
NEEGCRLGHM DYLFTKGCFE HIGHAIDSYT WGISWFGFAI LMWTLPVMLI

AMYFYTML
Length:258
Mass (Da):28,879
Last modified:July 1, 1997 - v1
Checksum:i79AF29E0DAEC4E44
GO
Isoform 2 (identifier: O00322-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     217-258: GCFEHIGHAI...LIAMYFYTML → AGVQWHNLSS...GVSRGLGLPS

Note: No experimental confirmation available.
Show »
Length:273
Mass (Da):30,020
Checksum:i509462D619800662
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti33 – 331S → A.
Corresponds to variant rs2267586 [ dbSNP | Ensembl ].
VAR_052332
Natural varianti257 – 2571M → T.1 Publication
Corresponds to variant rs2285421 [ dbSNP | Ensembl ].
VAR_020094

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei217 – 25842GCFEH…FYTML → AGVQWHNLSSLQRLPPGFKG FSHLSFQSSWDYRAASNTSA TPSTATRGVSRGLGLPS in isoform 2. 1 PublicationVSP_030005Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002115 Genomic DNA. Translation: AAB57631.1.
AC002314 Genomic DNA. Translation: AAB62736.1.
BC107097 mRNA. Translation: AAI07098.1.
BC107098 mRNA. Translation: AAI07099.1.
AF085807 mRNA. Translation: AAC34887.1.
CCDSiCCDS12470.1. [O00322-1]
CCDS62640.1. [O00322-2]
RefSeqiNP_001268372.1. NM_001281443.1. [O00322-2]
NP_008931.1. NM_007000.3. [O00322-1]
UniGeneiHs.159309.

Genome annotation databases

EnsembliENST00000222275; ENSP00000222275; ENSG00000105668. [O00322-1]
ENST00000379013; ENSP00000368298; ENSG00000105668. [O00322-2]
ENST00000616789; ENSP00000478942; ENSG00000105668. [O00322-2]
ENST00000617999; ENSP00000483176; ENSG00000105668. [O00322-1]
GeneIDi11045.
KEGGihsa:11045.
UCSCiuc002oaw.5. human. [O00322-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002115 Genomic DNA. Translation: AAB57631.1.
AC002314 Genomic DNA. Translation: AAB62736.1.
BC107097 mRNA. Translation: AAI07098.1.
BC107098 mRNA. Translation: AAI07099.1.
AF085807 mRNA. Translation: AAC34887.1.
CCDSiCCDS12470.1. [O00322-1]
CCDS62640.1. [O00322-2]
RefSeqiNP_001268372.1. NM_001281443.1. [O00322-2]
NP_008931.1. NM_007000.3. [O00322-1]
UniGeneiHs.159309.

3D structure databases

ProteinModelPortaliO00322.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116233. 80 interactions.
STRINGi9606.ENSP00000222275.

Polymorphism and mutation databases

BioMutaiUPK1A.

Proteomic databases

PaxDbiO00322.
PRIDEiO00322.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222275; ENSP00000222275; ENSG00000105668. [O00322-1]
ENST00000379013; ENSP00000368298; ENSG00000105668. [O00322-2]
ENST00000616789; ENSP00000478942; ENSG00000105668. [O00322-2]
ENST00000617999; ENSP00000483176; ENSG00000105668. [O00322-1]
GeneIDi11045.
KEGGihsa:11045.
UCSCiuc002oaw.5. human. [O00322-1]

Organism-specific databases

CTDi11045.
GeneCardsiUPK1A.
H-InvDBHIX0202895.
HGNCiHGNC:12577. UPK1A.
HPAiHPA049879.
MIMi611557. gene.
neXtProtiNX_O00322.
PharmGKBiPA37209.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3882. Eukaryota.
ENOG4111IRY. LUCA.
GeneTreeiENSGT00390000000126.
HOGENOMiHOG000230653.
HOVERGENiHBG054305.
InParanoidiO00322.
KOiK17347.
OMAiHVDYLFT.
OrthoDBiEOG76HQ28.
PhylomeDBiO00322.
TreeFamiTF335659.

Miscellaneous databases

GeneWikiiUPK1A.
GenomeRNAii11045.
NextBioi41976.
PROiO00322.
SOURCEiSearch...

Gene expression databases

BgeeiO00322.
CleanExiHS_UPK1A.
GenevisibleiO00322. HS.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANT THR-257.
  3. "Transcriptional control of the human urothelial-specific gene, uroplakin Ia."
    Hall G.D., Weeks R.J., Olsburgh J., Southgate J., Knowles M.A., Selby P.J., Chester J.D.
    Biochim. Biophys. Acta 1729:126-134(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-258 (ISOFORM 1), TISSUE SPECIFICITY.
    Tissue: Ureter.
  4. Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiUPK1A_HUMAN
AccessioniPrimary (citable) accession number: O00322
Secondary accession number(s): Q3KNU5, Q3KNU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: March 16, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.