O00268 (TAF4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription initiation factor TFIID subunit 4

Alternative name(s):
RNA polymerase II TBP-associated factor subunit C
TBP-associated factor 4
Transcription initiation factor TFIID 130 kDa subunit
Short name=TAF(II)130
Short name=TAFII-130
Short name=TAFII130
Transcription initiation factor TFIID 135 kDa subunit
Short name=TAF(II)135
Short name=TAFII-135
Short name=TAFII135

Gene names

Name:	TAF4
Synonyms:	TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1085 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Makes part of TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.
Subunit structure	TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H, TAF2, TAF4, TAF5, GCN5L2/GCN5, TAF10 and TRRAP. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with ATF7; the interaction inhibits ATF7-mediated tranactivation. Interacts with SV40 Large T antigen. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10
Subcellular location	Nucleus.
Sequence similarities	Belongs to the TAF4 family. Contains 1 TAFH (NHR1) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ovarian follicle development Inferred from electronic annotation. Source: Compara positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 12771217. Source: UniProtKB transcription elongation from RNA polymerase II promoter Traceable author statement. Source: Reactome transcription initiation from RNA polymerase II promoter Inferred from direct assay PubMed 9603525. Source: UniProtKB viral reproductive process Traceable author statement. Source: Reactome virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	MLL1 complex Inferred from direct assay Ref.9. Source: UniProtKB cytoplasm Inferred from direct assay. Source: HPA transcription factor TFIID complex Inferred from direct assay PubMed 14580349. Source: MGI transcription factor TFTC complex Inferred from direct assay Ref.7PubMed 9603525. Source: UniProtKB
Molecular_function	DNA binding Inferred from direct assay PubMed 15601843. Source: UniProtKB sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro transcription coactivator activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1085

1085

Transcription initiation factor TFIID subunit 4

PRO_0000118869

Regions

Domain

590 – 687

98

TAFH

Compositional bias

39 – 42

4

Poly-His

Compositional bias

52 – 57

6

Poly-Ala

Compositional bias

98 – 101

4

Poly-Gly

Compositional bias

142 – 148

7

Poly-Ala

Compositional bias

270 – 277

8

Poly-Pro

Compositional bias

333 – 339

7

Poly-Ala

Compositional bias

682 – 685

4

Poly-Pro

Compositional bias

810 – 815

6

Poly-Ala

Compositional bias

830 – 833

4

Poly-Asp

Natural variations

Natural variant

651

1

P → L.
Corresponds to variant rs6089604 [ dbSNP | Ensembl ].

VAR_052258

Experimental info

Sequence conflict

105 – 117

13

PGPPS…PLVPA → GRGLLQQRGGRES in AAC50901. Ref.5

Sequence conflict

136

1

S → A in CAA72189. Ref.1

Sequence conflict

136

1

S → A in AAC50901. Ref.5

Sequence conflict

186 – 187

2

Missing in CAA72189. Ref.1

Sequence conflict

186 – 187

2

Missing in AAC50901. Ref.5

Sequence conflict

235 – 266

32

Missing in AAC50901. Ref.5

Sequence conflict

295

1

P → L in AAC50901. Ref.5

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

1085

Helix Strand Turn

Details...

Helix

585 – 607

23

Beta strand

609 – 612

4

Helix

614 – 628

15

Helix

634 – 644

11

Helix

653 – 666

14

Helix

671 – 677

7

Helix

875 – 887

13

Turn

888 – 890

3

Helix

898 – 918

21

Sequences

	Sequence		Length	Mass (Da)
	O00268 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: BC2F5B5F143DB145	FASTA	1,085	110,114
	10 20 30 40 50 60 MAAGSDLLDE VFFNSEVDEK VVSDLVGSLE SQLAASAAHH HHLAPRTPEV RAAAAGALGN 70 80 90 100 110 120 HVVSGSPAGA AGAGPAAPAE GAPGAAPEPP PAGRARPGGG GPQRPGPPSP RRPLVPAGPA 130 140 150 160 170 180 PPAAKLRPPP EGSAGSCAPV PAAAAVAAGP EPAPAGPAKP AGPAALAARA GPGPGPGPGP 190 200 210 220 230 240 GPGPGPGKPA GPGAAQTLNG SAALLNSHHA AAPAVSLVNN GPAALLPLPK PAAPGTVIQT 250 260 270 280 290 300 PPFVGAAAPP APAAPSPPAA PAPAAPAAAP PPPPPAPATL ARPPGHPAGP PTAAPAVPPP 310 320 330 340 350 360 AAAQNGGSAG AAPAPAPAAG GPAGVSGQPG PGAAAAAPAP GVKAESPKRV VQAAPPAAQT 370 380 390 400 410 420 LAASGPASTA ASMVIGPTMQ GALPSPAAVP PPAPGTPTGL PKGAAGAVTQ SLSRTPTATT 430 440 450 460 470 480 SGIRATLTPT VLAPRLPQPP QNPTNIQNFQ LPPGMVLVRS ENGQLLMIPQ QALAQMQAQA 490 500 510 520 530 540 HAQPQTTMAP RPATPTSAPP VQISTVQAPG TPIIARQVTP TTIIKQVSQA QTTVQPSATL 550 560 570 580 590 600 QRSPGVQPQL VLGGAAQTAS LGTATAVQTG TPQRTVPGAT TTSSAATETM ENVKKCKNFL 610 620 630 640 650 660 STLIKLASSG KQSTETAANV KELVQNLLDG KIEAEDFTSR LYRELNSSPQ PYLVPFLKRS 670 680 690 700 710 720 LPALRQLTPD SAAFIQQSQQ QPPPPTSQAT TALTAVVLSS SVQRTAGKTA ATVTSALQPP 730 740 750 760 770 780 VLSLTQPTQV GVGKQGQPTP LVIQQPPKPG ALIRPPQVTL TQTPMVALRQ PHNRIMLTTP 790 800 810 820 830 840 QQIQLNPLQP VPVVKPAVLP GTKALSAVSA QAAAAQKNKL KEPGGGSFRD DDDINDVASM 850 860 870 880 890 900 AGVNLSEESA RILATNSELV GTLTRSCKDE TFLLQAPLQR RILEIGKKHG ITELHPDVVS 910 920 930 940 950 960 YVSHATQQRL QNLVEKISET AQQKNFSYKD DDRYEQASDV RAQLKFFEQL DQIEKQRKDE 970 980 990 1000 1010 1020 QEREILMRAA KSRSRQEDPE QLRLKQKAKE MQQQELAQMR QRDANLTALA AIGPRKKRKV 1030 1040 1050 1060 1070 1080 DCPGPGSGAE GSGPGSVVPG SSGVGTPRQF TRQRITRVNL RDLIFCLENE RETSHSLLLY KAFLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human TAF(II)135 potentiates transcriptional activation by the AF-2s of the retinoic acid, vitamin D3, and thyroid hormone receptors in mammalian cells." Mengus G., May M., Carre L., Chambon P., Davidson I. Genes Dev. 11:1381-1395(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	NIEHS SNPs program Submitted (MAY-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Molecular cloning and analysis of two subunits of the human TFIID complex: hTAFII130 and hTAFII100." Tanese N., Saluja D., Vassallo M.F., Chen J.-L., Admon A. Proc. Natl. Acad. Sci. U.S.A. 93:13611-13616(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 105-1085, PARTIAL PROTEIN SEQUENCE.
[6]	"TAF-like function of SV40 large T antigen." Damania B., Alwine J.C. Genes Dev. 10:1369-1381(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SV40 LARGE T ANTIGEN.
[7]	"Identification of TATA-binding protein-free TAFII-containing complex subunits suggests a role in nucleosome acetylation and signal transduction." Brand M., Yamamoto K., Staub A., Tora L. J. Biol. Chem. 274:18285-18289(1999) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX WITH TAF5L; TAF6L; TADA3L; SUPT3H; TAF2; TAF5; TRRAP; GCN5L2 AND TAF10.
[8]	"Novel subunits of the TATA binding protein free TAFII-containing transcription complex identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry following one-dimensional gel electrophoresis." Cavusoglu N., Brand M., Tora L., van Dorsselaer A. Proteomics 3:217-223(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TFTC-HAT COMPLEX, MASS SPECTROMETRY.
[9]	"Physical association and coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16 acetyltransferase MOF." Dou Y., Milne T.A., Tackett A.J., Smith E.R., Fukuda A., Wysocka J., Allis C.D., Chait B.T., Hess J.L., Roeder R.G. Cell 121:873-885(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE MLL1/MLL COMPLEX.
[10]	"A functional interaction between ATF7 and TAF12 that is modulated by TAF4." Hamard P.J., Dalbies-Tran R., Hauss C., Davidson I., Kedinger C., Chatton B. Oncogene 24:3472-3483(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ATF7.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[12]	"The human TFIID components TAF(II)135 and TAF(II)20 and the yeast SAGA components ADA1 and TAF(II)68 heterodimerize to form histone-like pairs." Gangloff Y.-G., Werten S., Romier C., Carre L., Poch O., Moras D., Davidson I. Mol. Cell. Biol. 20:340-351(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 872-920 IN COMPLEX WITH TAF12.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y11354 mRNA. Translation: CAA72189.1.
AY623115 Genomic DNA. Translation: AAT38111.1.
AL109911, AL137077 Genomic DNA. Translation: CAI19182.1.
AL137077, AL109911 Genomic DNA. Translation: CAI11045.1.
CH471077 Genomic DNA. Translation: EAW75407.1.
U75308 mRNA. Translation: AAC50901.1.

IPI

IPI00413755.

RefSeq

NP_003176.2. NM_003185.3.

UniGene

Hs.18857.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H3O	X-ray	2.30	A/C	872-945	[»]
2P6V	X-ray	2.00	A	575-688	[»]

ProteinModelPortal

O00268.

ModBase

Search...

Protein-protein interaction databases

IntAct

O00268. 6 interactions.

MINT

MINT-236922.

STRING

9606.ENSP00000252996.

PTM databases

PhosphoSite

O00268.

Proteomic databases

PaxDb

O00268.

PRIDE

O00268.

Protocols and materials databases

DNASU

6874.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000252996; ENSP00000252996; ENSG00000130699.

GeneID

6874.

KEGG

hsa:6874.

UCSC

uc002ybs.3. human.

Organism-specific databases

CTD

6874.

GeneCards

GC20M060529.

H-InvDB

HIX0015971.
HIX0174705.

HGNC

HGNC:11537. TAF4.

HPA

CAB031484.
HPA008599.

MIM

601796. gene.

neXtProt

NX_O00268.

PharmGKB

PA36312.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG237595.

HOGENOM

HOG000154502.

HOVERGEN

HBG058585.

InParanoid

O00268.

KO

K03129.

OMA

LDEVFFN.

OrthoDB

EOG4CJVH7.

Enzyme and pathway databases

Reactome

REACT_116125. Disease.
REACT_1788. Transcription.
REACT_71. Gene Expression.

Gene expression databases

ArrayExpress

O00268.

Bgee

O00268.

CleanEx

HS_TAF4.

Genevestigator

O00268.

GermOnline

ENSG00000130699. Homo sapiens.

Family and domain databases

Gene3D

1.10.20.10. 1 hit.

InterPro

IPR009072. Histone-fold.
IPR007900. TAF4.
IPR003894. TAFH_NHR1.
[Graphical view]

Pfam

PF05236. TAF4. 1 hit.
PF07531. TAFH. 1 hit.
[Graphical view]

SMART

SM00549. TAFH. 1 hit.
[Graphical view]

SUPFAM

SSF47113. Histone-fold. 1 hit.

PROSITE

PS51119. TAFH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

TAF4. human.

EvolutionaryTrace

O00268.

GenomeRNAi

6874.

NextBio

26837.

SOURCE

Search...

Entry information

Entry name

TAF4_HUMAN

Accession

Primary (citable) accession number: O00268
Secondary accession number(s): A6NGD9 , Q5TBP6, Q99721, Q9BR40, Q9BX42

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 7, 2004
Last modified:	May 29, 2013
This is version 143 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.