Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Transcription initiation factor TFIID subunit 4

Gene

TAF4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the TFIID complex, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Potentiates transcriptional activation by the AF-2S of the retinoic acid, vitamin D3 and thyroid hormone.

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • transcription coactivator activity Source: ProtInc
  • transcription factor activity, sequence-specific DNA binding Source: InterPro
  • transcription factor binding Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130699-MONOMER.
ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
SIGNORiO00268.

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription initiation factor TFIID subunit 4
Alternative name(s):
RNA polymerase II TBP-associated factor subunit C
TBP-associated factor 4
Transcription initiation factor TFIID 130 kDa subunit
Short name:
TAF(II)130
Short name:
TAFII-130
Short name:
TAFII130
Transcription initiation factor TFIID 135 kDa subunit
Short name:
TAF(II)135
Short name:
TAFII-135
Short name:
TAFII135
Gene namesi
Name:TAF4
Synonyms:TAF2C, TAF2C1, TAF4A, TAFII130, TAFII135
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

HGNCiHGNC:11537. TAF4.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: HPA
  • MLL1 complex Source: UniProtKB
  • nuclear chromatin Source: ParkinsonsUK-UCL
  • nucleoplasm Source: HPA
  • transcription factor TFIID complex Source: MGI
  • transcription factor TFTC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi6874.
OpenTargetsiENSG00000130699.
PharmGKBiPA36312.

Polymorphism and mutation databases

BioMutaiTAF4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001188691 – 1085Transcription initiation factor TFIID subunit 4Add BLAST1085

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei109PhosphoserineCombined sources1
Modified residuei424Asymmetric dimethylarginineCombined sources1
Modified residuei435Asymmetric dimethylarginineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiO00268.
MaxQBiO00268.
PaxDbiO00268.
PeptideAtlasiO00268.
PRIDEiO00268.

PTM databases

iPTMnetiO00268.
PhosphoSitePlusiO00268.

Expressioni

Gene expression databases

BgeeiENSG00000130699.
CleanExiHS_TAF4.
ExpressionAtlasiO00268. baseline and differential.
GenevisibleiO00268. HS.

Organism-specific databases

HPAiCAB031484.
HPA008599.

Interactioni

Subunit structurei

TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Component of the TFTC-HAT complex, at least composed of TAF5L, TAF6L, TADA3L, SUPT3H, TAF2, TAF4, TAF5, GCN5L2/GCN5, TAF10 and TRRAP. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with ATF7; the interaction inhibits ATF7-mediated tranactivation. Interacts with SV40 Large T antigen.6 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112737. 51 interactors.
DIPiDIP-35350N.
IntActiO00268. 13 interactors.
MINTiMINT-236922.
STRINGi9606.ENSP00000252996.

Structurei

Secondary structure

11085
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi585 – 607Combined sources23
Beta strandi609 – 612Combined sources4
Helixi614 – 628Combined sources15
Helixi634 – 644Combined sources11
Helixi653 – 666Combined sources14
Helixi671 – 677Combined sources7
Helixi875 – 887Combined sources13
Turni888 – 890Combined sources3
Helixi898 – 918Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3OX-ray2.30A/C872-945[»]
2P6VX-ray2.00A575-679[»]
ProteinModelPortaliO00268.
SMRiO00268.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00268.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini590 – 687TAFHPROSITE-ProRule annotationAdd BLAST98

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 42Poly-His4
Compositional biasi52 – 57Poly-Ala6
Compositional biasi98 – 101Poly-Gly4
Compositional biasi142 – 148Poly-Ala7
Compositional biasi270 – 277Poly-Pro8
Compositional biasi333 – 339Poly-Ala7
Compositional biasi682 – 685Poly-Pro4
Compositional biasi810 – 815Poly-Ala6
Compositional biasi830 – 833Poly-Asp4

Sequence similaritiesi

Belongs to the TAF4 family.Curated
Contains 1 TAFH (NHR1) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2341. Eukaryota.
ENOG410XQS6. LUCA.
GeneTreeiENSGT00390000011620.
HOGENOMiHOG000154502.
HOVERGENiHBG058585.
InParanoidiO00268.
KOiK03129.
OMAiRPPIHTA.
OrthoDBiEOG091G0GOS.
PhylomeDBiO00268.
TreeFamiTF316520.

Family and domain databases

CDDicd08045. TAF4. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007900. TAF4.
IPR003894. TAFH_NHR1.
[Graphical view]
PfamiPF05236. TAF4. 1 hit.
PF07531. TAFH. 1 hit.
[Graphical view]
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS51119. TAFH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O00268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGSDLLDE VFFNSEVDEK VVSDLVGSLE SQLAASAAHH HHLAPRTPEV
60 70 80 90 100
RAAAAGALGN HVVSGSPAGA AGAGPAAPAE GAPGAAPEPP PAGRARPGGG
110 120 130 140 150
GPQRPGPPSP RRPLVPAGPA PPAAKLRPPP EGSAGSCAPV PAAAAVAAGP
160 170 180 190 200
EPAPAGPAKP AGPAALAARA GPGPGPGPGP GPGPGPGKPA GPGAAQTLNG
210 220 230 240 250
SAALLNSHHA AAPAVSLVNN GPAALLPLPK PAAPGTVIQT PPFVGAAAPP
260 270 280 290 300
APAAPSPPAA PAPAAPAAAP PPPPPAPATL ARPPGHPAGP PTAAPAVPPP
310 320 330 340 350
AAAQNGGSAG AAPAPAPAAG GPAGVSGQPG PGAAAAAPAP GVKAESPKRV
360 370 380 390 400
VQAAPPAAQT LAASGPASTA ASMVIGPTMQ GALPSPAAVP PPAPGTPTGL
410 420 430 440 450
PKGAAGAVTQ SLSRTPTATT SGIRATLTPT VLAPRLPQPP QNPTNIQNFQ
460 470 480 490 500
LPPGMVLVRS ENGQLLMIPQ QALAQMQAQA HAQPQTTMAP RPATPTSAPP
510 520 530 540 550
VQISTVQAPG TPIIARQVTP TTIIKQVSQA QTTVQPSATL QRSPGVQPQL
560 570 580 590 600
VLGGAAQTAS LGTATAVQTG TPQRTVPGAT TTSSAATETM ENVKKCKNFL
610 620 630 640 650
STLIKLASSG KQSTETAANV KELVQNLLDG KIEAEDFTSR LYRELNSSPQ
660 670 680 690 700
PYLVPFLKRS LPALRQLTPD SAAFIQQSQQ QPPPPTSQAT TALTAVVLSS
710 720 730 740 750
SVQRTAGKTA ATVTSALQPP VLSLTQPTQV GVGKQGQPTP LVIQQPPKPG
760 770 780 790 800
ALIRPPQVTL TQTPMVALRQ PHNRIMLTTP QQIQLNPLQP VPVVKPAVLP
810 820 830 840 850
GTKALSAVSA QAAAAQKNKL KEPGGGSFRD DDDINDVASM AGVNLSEESA
860 870 880 890 900
RILATNSELV GTLTRSCKDE TFLLQAPLQR RILEIGKKHG ITELHPDVVS
910 920 930 940 950
YVSHATQQRL QNLVEKISET AQQKNFSYKD DDRYEQASDV RAQLKFFEQL
960 970 980 990 1000
DQIEKQRKDE QEREILMRAA KSRSRQEDPE QLRLKQKAKE MQQQELAQMR
1010 1020 1030 1040 1050
QRDANLTALA AIGPRKKRKV DCPGPGSGAE GSGPGSVVPG SSGVGTPRQF
1060 1070 1080
TRQRITRVNL RDLIFCLENE RETSHSLLLY KAFLK
Length:1,085
Mass (Da):110,114
Last modified:December 7, 2004 - v2
Checksum:iBC2F5B5F143DB145
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105 – 117PGPPS…PLVPA → GRGLLQQRGGRES in AAC50901 (PubMed:8942982).CuratedAdd BLAST13
Sequence conflicti136S → A in CAA72189 (PubMed:9192867).Curated1
Sequence conflicti136S → A in AAC50901 (PubMed:8942982).Curated1
Sequence conflicti186 – 187Missing in CAA72189 (PubMed:9192867).Curated2
Sequence conflicti186 – 187Missing in AAC50901 (PubMed:8942982).Curated2
Sequence conflicti235 – 266Missing in AAC50901 (PubMed:8942982).CuratedAdd BLAST32
Sequence conflicti295P → L in AAC50901 (PubMed:8942982).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052258651P → L.Corresponds to variant rs6089604dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11354 mRNA. Translation: CAA72189.1.
AY623115 Genomic DNA. Translation: AAT38111.1.
AL109911, AL137077 Genomic DNA. Translation: CAI19182.1.
AL137077, AL109911 Genomic DNA. Translation: CAI11045.1.
CH471077 Genomic DNA. Translation: EAW75407.1.
U75308 mRNA. Translation: AAC50901.1.
CCDSiCCDS33500.1.
RefSeqiNP_003176.2. NM_003185.3.
UniGeneiHs.18857.

Genome annotation databases

EnsembliENST00000252996; ENSP00000252996; ENSG00000130699.
GeneIDi6874.
KEGGihsa:6874.
UCSCiuc002ybs.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y11354 mRNA. Translation: CAA72189.1.
AY623115 Genomic DNA. Translation: AAT38111.1.
AL109911, AL137077 Genomic DNA. Translation: CAI19182.1.
AL137077, AL109911 Genomic DNA. Translation: CAI11045.1.
CH471077 Genomic DNA. Translation: EAW75407.1.
U75308 mRNA. Translation: AAC50901.1.
CCDSiCCDS33500.1.
RefSeqiNP_003176.2. NM_003185.3.
UniGeneiHs.18857.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1H3OX-ray2.30A/C872-945[»]
2P6VX-ray2.00A575-679[»]
ProteinModelPortaliO00268.
SMRiO00268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112737. 51 interactors.
DIPiDIP-35350N.
IntActiO00268. 13 interactors.
MINTiMINT-236922.
STRINGi9606.ENSP00000252996.

PTM databases

iPTMnetiO00268.
PhosphoSitePlusiO00268.

Polymorphism and mutation databases

BioMutaiTAF4.

Proteomic databases

EPDiO00268.
MaxQBiO00268.
PaxDbiO00268.
PeptideAtlasiO00268.
PRIDEiO00268.

Protocols and materials databases

DNASUi6874.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000252996; ENSP00000252996; ENSG00000130699.
GeneIDi6874.
KEGGihsa:6874.
UCSCiuc002ybs.3. human.

Organism-specific databases

CTDi6874.
DisGeNETi6874.
GeneCardsiTAF4.
H-InvDBHIX0015971.
HIX0174705.
HGNCiHGNC:11537. TAF4.
HPAiCAB031484.
HPA008599.
MIMi601796. gene.
neXtProtiNX_O00268.
OpenTargetsiENSG00000130699.
PharmGKBiPA36312.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2341. Eukaryota.
ENOG410XQS6. LUCA.
GeneTreeiENSGT00390000011620.
HOGENOMiHOG000154502.
HOVERGENiHBG058585.
InParanoidiO00268.
KOiK03129.
OMAiRPPIHTA.
OrthoDBiEOG091G0GOS.
PhylomeDBiO00268.
TreeFamiTF316520.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000130699-MONOMER.
ReactomeiR-HSA-167161. HIV Transcription Initiation.
R-HSA-167162. RNA Polymerase II HIV Promoter Escape.
R-HSA-167172. Transcription of the HIV genome.
R-HSA-674695. RNA Polymerase II Pre-transcription Events.
R-HSA-6804756. Regulation of TP53 Activity through Phosphorylation.
R-HSA-73776. RNA Polymerase II Promoter Escape.
R-HSA-73779. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
R-HSA-75953. RNA Polymerase II Transcription Initiation.
R-HSA-76042. RNA Polymerase II Transcription Initiation And Promoter Clearance.
SIGNORiO00268.

Miscellaneous databases

ChiTaRSiTAF4. human.
EvolutionaryTraceiO00268.
GeneWikiiTAF4.
GenomeRNAii6874.
PROiO00268.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000130699.
CleanExiHS_TAF4.
ExpressionAtlasiO00268. baseline and differential.
GenevisibleiO00268. HS.

Family and domain databases

CDDicd08045. TAF4. 1 hit.
Gene3Di1.10.20.10. 1 hit.
InterProiIPR009072. Histone-fold.
IPR007900. TAF4.
IPR003894. TAFH_NHR1.
[Graphical view]
PfamiPF05236. TAF4. 1 hit.
PF07531. TAFH. 1 hit.
[Graphical view]
SMARTiSM00549. TAFH. 1 hit.
[Graphical view]
SUPFAMiSSF47113. SSF47113. 1 hit.
PROSITEiPS51119. TAFH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTAF4_HUMAN
AccessioniPrimary (citable) accession number: O00268
Secondary accession number(s): A6NGD9
, Q5TBP6, Q99721, Q9BR40, Q9BX42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 7, 2004
Last modified: November 30, 2016
This is version 177 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.