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O00258 (WRB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tail-anchored protein insertion receptor WRB
Alternative name(s):
Congenital heart disease 5 protein
Tryptophan-rich basic protein
Short name=WRB
Gene names
Name:WRB
Synonyms:CHD5
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length174 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for ASNA1/TRC40-mediated insertion of tail-anchored (TA) proteins into the ER membrane. Ref.6

Subunit structure

Interacts with ASNA1/TRC40. Ref.6

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1 Ref.6.

Sequence similarities

Belongs to the WRB/GET1 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O00258-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O00258-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 174174Tail-anchored protein insertion receptor WRB
PRO_0000065979

Regions

Topological domain1 – 88Lumenal Potential
Transmembrane9 – 2921Helical; Potential
Topological domain30 – 9970Cytoplasmic Potential
Transmembrane100 – 12021Helical; Potential
Topological domain121 – 14828Lumenal Potential
Transmembrane149 – 16921Helical; Potential
Topological domain170 – 1745Cytoplasmic Potential
Region39 – 9759Interaction with ASNA1/TRC40
Coiled coil39 – 9456 Potential

Natural variations

Alternative sequence1 – 3434Missing in isoform 2.
VSP_043081
Natural variant1101V → I.
Corresponds to variant rs35946782 [ dbSNP | Ensembl ].
VAR_051491

Experimental info

Sequence conflict311F → I in CAA73081. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: A01F11CC564EAB6E

FASTA17419,780
        10         20         30         40         50         60 
MSSAAADHWA WLLVLSFVFG CNVLRILLPS FSSFMSRVLQ KDAEQESQMR AEIQDMKQEL 

        70         80         90        100        110        120 
STVNMMDEFA RYARLERKIN KMTDKLKTHV KARTAQLAKI KWVISVAFYV LQAALMISLI 

       130        140        150        160        170 
WKYYSVPVAV VPSKWITPLD RLVAFPTRVA GGVGITCWIL VCNKVVAIVL HPFS 

« Hide

Isoform 2 [UniParc].

Checksum: 601B094EF9F52C95
Show »

FASTA14016,011

References

« Hide 'large scale' references
[1]"Identification and characterization of a new human cDNA from chromosome 21q22.3 encoding a basic nuclear protein."
Egeo A., Mazzocco M., Sotgia F., Arrigo P., Oliva R., Bergonon S., Nizetic D., Rasore-Quartino A., Scartezzini P.
Hum. Genet. 102:289-293(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION.
Tissue: Heart.
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[3]"The DNA sequence of human chromosome 21."
Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. expand/collapse author list , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L.
Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Ovary.
[6]"WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane."
Vilardi F., Lorenz H., Dobberstein B.
J. Cell Sci. 124:1301-1307(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TOPOLOGY, INTERACTION WITH ASNA1/TRC40, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y12478 mRNA. Translation: CAA73081.1.
AK293113 mRNA. Translation: BAF85802.1.
AK299144 mRNA. Translation: BAG61196.1.
AF064861 Genomic DNA. No translation available.
AF121781 Genomic DNA. No translation available.
AL163279 Genomic DNA. Translation: CAB90454.1.
CH471079 Genomic DNA. Translation: EAX09645.1.
CH471079 Genomic DNA. Translation: EAX09647.1.
CH471079 Genomic DNA. Translation: EAX09649.1.
BC012415 mRNA. Translation: AAH12415.1.
CCDSCCDS13664.1. [O00258-1]
CCDS54485.1. [O00258-2]
RefSeqNP_001139690.1. NM_001146218.1. [O00258-2]
NP_004618.2. NM_004627.4. [O00258-1]
XP_005261116.1. XM_005261059.2. [O00258-2]
XP_005261117.1. XM_005261060.2. [O00258-2]
XP_005261118.1. XM_005261061.2. [O00258-2]
UniGeneHs.198308.
Hs.744958.

3D structure databases

ProteinModelPortalO00258.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid113322. 2 interactions.
STRING9606.ENSP00000327716.

Protein family/group databases

TCDB3.A.19.1.1. the tms recognition/insertion complex (trc) family.

PTM databases

PhosphoSiteO00258.

Proteomic databases

MaxQBO00258.
PaxDbO00258.
PRIDEO00258.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000333781; ENSP00000327716; ENSG00000182093. [O00258-1]
ENST00000380708; ENSP00000370084; ENSG00000182093. [O00258-2]
ENST00000398753; ENSP00000381737; ENSG00000182093. [O00258-2]
GeneID7485.
KEGGhsa:7485.
UCSCuc002yxs.3. human. [O00258-1]

Organism-specific databases

CTD7485.
GeneCardsGC21P040752.
HGNCHGNC:12790. WRB.
HPAHPA018527.
MIM602915. gene.
neXtProtNX_O00258.
PharmGKBPA37391.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG287621.
HOGENOMHOG000013223.
HOVERGENHBG010388.
InParanoidO00258.
OMAITCWLVV.
OrthoDBEOG7TJ3KG.
PhylomeDBO00258.
TreeFamTF314708.

Gene expression databases

ArrayExpressO00258.
BgeeO00258.
CleanExHS_CHD5.
HS_WRB.
GenevestigatorO00258.

Family and domain databases

InterProIPR028945. WRB/Get1.
[Graphical view]
PfamPF04420. CHD5. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi7485.
NextBio29322.
PROO00258.
SOURCESearch...

Entry information

Entry nameWRB_HUMAN
AccessionPrimary (citable) accession number: O00258
Secondary accession number(s): A8KAP8 expand/collapse secondary AC list , A8MQ44, D3DSH9, O60740
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 21

Human chromosome 21: entries, gene names and cross-references to MIM