O00258 (WRB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tail-anchored protein insertion receptor WRB

Alternative name(s):
Congenital heart disease 5 protein
Tryptophan-rich basic protein
Short name=WRB

Gene names

Name:	WRB
Synonyms:	CHD5

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	174 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for ASNA1/TRC40-mediated insertion of tail-anchored (TA) proteins into the ER membrane. Ref.6
Subunit structure	Interacts with ASNA1/TRC40. Ref.6
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein Ref.1 Ref.6.
Sequence similarities	Belongs to the WRB/GET1 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW nucleolus Inferred from direct assay. Source: HPA
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O00258-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O00258-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-34: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 174	174	Tail-anchored protein insertion receptor WRB		PRO_0000065979
Regions
	Topological domain	1 – 8	8	Lumenal Potential
	Transmembrane	9 – 29	21	Helical; Potential
	Topological domain	30 – 99	70	Cytoplasmic Potential
	Transmembrane	100 – 120	21	Helical; Potential
	Topological domain	121 – 148	28	Lumenal Potential
	Transmembrane	149 – 169	21	Helical; Potential
	Topological domain	170 – 174	5	Cytoplasmic Potential
	Region	39 – 97	59	Interaction with ASNA1/TRC40
	Coiled coil	39 – 94	56	Potential
Natural variations
	Alternative sequence	1 – 34	34	Missing in isoform 2.		VSP_043081
	Natural variant	110	1	V → I. Corresponds to variant rs35946782 [ dbSNP | Ensembl ].		VAR_051491
Experimental info
	Sequence conflict	31	1	F → I in CAA73081. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: A01F11CC564EAB6E	FASTA	174	19,780
	10 20 30 40 50 60 MSSAAADHWA WLLVLSFVFG CNVLRILLPS FSSFMSRVLQ KDAEQESQMR AEIQDMKQEL 70 80 90 100 110 120 STVNMMDEFA RYARLERKIN KMTDKLKTHV KARTAQLAKI KWVISVAFYV LQAALMISLI 130 140 150 160 170 WKYYSVPVAV VPSKWITPLD RLVAFPTRVA GGVGITCWIL VCNKVVAIVL HPFS
	Isoform 2 [UniParc]. Checksum: 601B094EF9F52C95	FASTA	140	16,011
	10 20 30 40 50 60 MSRVLQKDAE QESQMRAEIQ DMKQELSTVN MMDEFARYAR LERKINKMTD KLKTHVKART 70 80 90 100 110 120 AQLAKIKWVI SVAFYVLQAA LMISLIWKYY SVPVAVVPSK WITPLDRLVA FPTRVAGGVG 130 140 ITCWILVCNK VVAIVLHPFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a new human cDNA from chromosome 21q22.3 encoding a basic nuclear protein." Egeo A., Mazzocco M., Sotgia F., Arrigo P., Oliva R., Bergonon S., Nizetic D., Rasore-Quartino A., Scartezzini P. Hum. Genet. 102:289-293(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION. Tissue: Heart.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[3]	"The DNA sequence of human chromosome 21." Hattori M., Fujiyama A., Taylor T.D., Watanabe H., Yada T., Park H.-S., Toyoda A., Ishii K., Totoki Y., Choi D.-K., Groner Y., Soeda E., Ohki M., Takagi T., Sakaki Y., Taudien S., Blechschmidt K., Polley A. , Menzel U., Delabar J., Kumpf K., Lehmann R., Patterson D., Reichwald K., Rump A., Schillhabel M., Schudy A., Zimmermann W., Rosenthal A., Kudoh J., Shibuya K., Kawasaki K., Asakawa S., Shintani A., Sasaki T., Nagamine K., Mitsuyama S., Antonarakis S.E., Minoshima S., Shimizu N., Nordsiek G., Hornischer K., Brandt P., Scharfe M., Schoen O., Desario A., Reichelt J., Kauer G., Bloecker H., Ramser J., Beck A., Klages S., Hennig S., Riesselmann L., Dagand E., Wehrmeyer S., Borzym K., Gardiner K., Nizetic D., Francis F., Lehrach H., Reinhardt R., Yaspo M.-L. Nature 405:311-319(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Ovary.
[6]	"WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane." Vilardi F., Lorenz H., Dobberstein B. J. Cell Sci. 124:1301-1307(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TOPOLOGY, INTERACTION WITH ASNA1/TRC40, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y12478 mRNA. Translation: CAA73081.1. AK293113 mRNA. Translation: BAF85802.1. AK299144 mRNA. Translation: BAG61196.1. AF064861 Genomic DNA. No translation available. AF121781 Genomic DNA. No translation available. AL163279 Genomic DNA. Translation: CAB90454.1. CH471079 Genomic DNA. Translation: EAX09645.1. CH471079 Genomic DNA. Translation: EAX09647.1. CH471079 Genomic DNA. Translation: EAX09649.1. BC012415 mRNA. Translation: AAH12415.1.
IPI	IPI00010873. IPI00658165.
RefSeq	NP_001139690.1. NM_001146218.1. NP_004618.2. NM_004627.4.
UniGene	Hs.198308.
3D structure databases
ProteinModelPortal	O00258.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000327716.
PTM databases
PhosphoSite	O00258.
Proteomic databases
PaxDb	O00258.
PRIDE	O00258.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000333781; ENSP00000327716; ENSG00000182093. ENST00000380708; ENSP00000370084; ENSG00000182093. ENST00000398753; ENSP00000381737; ENSG00000182093.
GeneID	7485.
KEGG	hsa:7485.
UCSC	uc002yxs.3. human.
Organism-specific databases
CTD	7485.
GeneCards	GC21P040752.
HGNC	HGNC:12790. WRB.
HPA	HPA018527.
MIM	602915. gene.
neXtProt	NX_O00258.
PharmGKB	PA37391.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG287621.
HOGENOM	HOG000013223.
HOVERGEN	HBG010388.
InParanoid	O00258.
OMA	ITCWLVV.
OrthoDB	EOG4KSPKW.
PhylomeDB	O00258.
Gene expression databases
ArrayExpress	O00258.
Bgee	O00258.
CleanEx	HS_CHD5. HS_WRB.
Genevestigator	O00258.
GermOnline	ENSG00000182093. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other
GenomeRNAi	7485.
NextBio	29322.
SOURCE	Search...

Entry information

Entry name

WRB_HUMAN

Accession

Primary (citable) accession number: O00258
Secondary accession number(s): A8KAP8 , A8MQ44, D3DSH9, O60740

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.