##gff-version 3 O00257 UniProtKB Chain 1 560 . . . ID=PRO_0000080206;Note=E3 SUMO-protein ligase CBX4 O00257 UniProtKB Domain 11 69 . . . Note=Chromo;Ontology_term=ECO:0000255;evidence=ECO:0000255|PROSITE-ProRule:PRU00053 O00257 UniProtKB Region 1 539 . . . Note=Interaction with BMI1 O00257 UniProtKB Region 1 75 . . . Note=Involved in interaction with H3C15 and H3C1;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:18927235;Dbxref=PMID:18927235 O00257 UniProtKB Region 92 152 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O00257 UniProtKB Region 217 243 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O00257 UniProtKB Region 281 404 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O00257 UniProtKB Region 509 528 . . . Note=Disordered;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O00257 UniProtKB Region 531 556 . . . Note=Involved in interaction with H3C15 and RNF2;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:18927235;Dbxref=PMID:18927235 O00257 UniProtKB Region 540 560 . . . Note=Interaction with RNF2 O00257 UniProtKB Compositional bias 92 106 . . . Note=Polar residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O00257 UniProtKB Compositional bias 283 335 . . . Note=Basic and acidic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O00257 UniProtKB Compositional bias 380 404 . . . Note=Basic residues;Ontology_term=ECO:0000256;evidence=ECO:0000256|SAM:MobiDB-lite O00257 UniProtKB Modified residue 149 149 . . . Note=N6-acetyllysine%3B alternate;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:19608861;Dbxref=PMID:19608861 O00257 UniProtKB Modified residue 182 182 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:23186163;Dbxref=PMID:23186163 O00257 UniProtKB Modified residue 467 467 . . . Note=Phosphoserine;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:24275569;Dbxref=PMID:24275569 O00257 UniProtKB Modified residue 497 497 . . . Note=Phosphothreonine%3B by HIPK2;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17018294;Dbxref=PMID:17018294 O00257 UniProtKB Cross-link 77 77 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 106 106 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:25218447,ECO:0007744|PubMed:28112733;Dbxref=PMID:25218447,PMID:28112733 O00257 UniProtKB Cross-link 114 114 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:25218447,ECO:0007744|PubMed:28112733;Dbxref=PMID:25218447,PMID:28112733 O00257 UniProtKB Cross-link 125 125 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 149 149 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)%3B alternate;Ontology_term=ECO:0007744,ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:25218447,ECO:0007744|PubMed:25772364,ECO:0007744|PubMed:28112733;Dbxref=PMID:25218447,PMID:25772364,PMID:28112733 O00257 UniProtKB Cross-link 157 157 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 167 167 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 178 178 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 191 191 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 205 205 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:25218447,ECO:0007744|PubMed:28112733;Dbxref=PMID:25218447,PMID:28112733 O00257 UniProtKB Cross-link 212 212 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744,ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:25218447,ECO:0007744|PubMed:25772364,ECO:0007744|PubMed:28112733;Dbxref=PMID:25218447,PMID:25772364,PMID:28112733 O00257 UniProtKB Cross-link 223 223 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 249 249 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 268 268 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 278 278 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 280 280 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744,ECO:0007744,ECO:0007744,ECO:0007744;evidence=ECO:0007744|PubMed:25218447,ECO:0007744|PubMed:25755297,ECO:0007744|PubMed:25772364,ECO:0007744|PubMed:28112733;Dbxref=PMID:25218447,PMID:25755297,PMID:25772364,PMID:28112733 O00257 UniProtKB Cross-link 320 320 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 352 352 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 365 365 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2);Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Cross-link 494 494 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)%3B alternate;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:15592428;Dbxref=PMID:15592428 O00257 UniProtKB Cross-link 494 494 . . . Note=Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)%3B alternate;Ontology_term=ECO:0007744;evidence=ECO:0007744|PubMed:28112733;Dbxref=PMID:28112733 O00257 UniProtKB Alternative sequence 127 396 . . . ID=VSP_041599;Note=In isoform 2. Missing;Ontology_term=ECO:0000303;evidence=ECO:0000303|Ref.3 O00257 UniProtKB Mutagenesis 16 16 . . . Note=Reduced interaction with H3C15%2C H3C1 and RNF2. I->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:18927235;Dbxref=PMID:18927235 O00257 UniProtKB Mutagenesis 434 434 . . . Note=Abolishes interaction with YWHAZ and YWHAE%3B impairs interaction with PCGF6 and BMI1%3B no effect on interaction with RNF2. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:21282530;Dbxref=PMID:21282530 O00257 UniProtKB Mutagenesis 494 494 . . . Note=No effect on ZNF131 sumoylation. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22467880;Dbxref=PMID:22467880 O00257 UniProtKB Mutagenesis 497 497 . . . Note=Small decrease in ZNF131 sumoylation. T->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22467880;Dbxref=PMID:22467880 O00257 UniProtKB Sequence conflict 137 138 . . . Note=Missing;Ontology_term=ECO:0000305;evidence=ECO:0000305 O00257 UniProtKB Sequence conflict 142 142 . . . Note=P->R;Ontology_term=ECO:0000305;evidence=ECO:0000305 O00257 UniProtKB Sequence conflict 458 458 . . . Note=P->R;Ontology_term=ECO:0000305;evidence=ECO:0000305 O00257 UniProtKB Sequence conflict 477 477 . . . Note=C->S;Ontology_term=ECO:0000305;evidence=ECO:0000305 O00257 UniProtKB Sequence conflict 480 480 . . . Note=T->S;Ontology_term=ECO:0000305;evidence=ECO:0000305 O00257 UniProtKB Sequence conflict 505 505 . . . Note=V->VAA;Ontology_term=ECO:0000305;evidence=ECO:0000305 O00257 UniProtKB Beta strand 13 22 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I8Z O00257 UniProtKB Beta strand 25 32 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I8Z O00257 UniProtKB Helix 37 39 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I8Z O00257 UniProtKB Beta strand 41 44 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I8Z O00257 UniProtKB Helix 45 48 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I8Z O00257 UniProtKB Helix 51 53 . . . Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3I8Z