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Protein

Proteinase-activated receptor 3

Gene

F2RL2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.1 Publication

GO - Molecular functioni

  • G-protein coupled receptor activity Source: Reactome
  • phosphatidylinositol phospholipase C activity Source: ProtInc
  • thrombin-activated receptor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Blood coagulation, Hemostasis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164220-MONOMER.
ReactomeiR-HSA-375276. Peptide ligand-binding receptors.
R-HSA-416476. G alpha (q) signalling events.
R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs).
SIGNORiO00254.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase-activated receptor 3
Short name:
PAR-3
Alternative name(s):
Coagulation factor II receptor-like 2
Thrombin receptor-like 2
Gene namesi
Name:F2RL2
Synonyms:PAR3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

HGNCiHGNC:3539. F2RL2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini39 – 94ExtracellularSequence analysisAdd BLAST56
Transmembranei95 – 120Helical; Name=1Sequence analysisAdd BLAST26
Topological domaini121 – 128CytoplasmicSequence analysis8
Transmembranei129 – 148Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini149 – 167ExtracellularSequence analysisAdd BLAST19
Transmembranei168 – 189Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini190 – 206CytoplasmicSequence analysisAdd BLAST17
Transmembranei207 – 230Helical; Name=4Sequence analysisAdd BLAST24
Topological domaini231 – 260ExtracellularSequence analysisAdd BLAST30
Transmembranei261 – 280Helical; Name=5Sequence analysisAdd BLAST20
Topological domaini281 – 297CytoplasmicSequence analysisAdd BLAST17
Transmembranei298 – 322Helical; Name=6Sequence analysisAdd BLAST25
Topological domaini323 – 336ExtracellularSequence analysisAdd BLAST14
Transmembranei337 – 361Helical; Name=7Sequence analysisAdd BLAST25
Topological domaini362 – 374CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

  • apical plasma membrane Source: Ensembl
  • extracellular region Source: Reactome
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi39T → P: No proteolytic cleavage by thrombin. 1 Publication1
Mutagenesisi40F → A: Altered signal upon thrombin cleavage. 1 Publication1

Organism-specific databases

DisGeNETi2151.
OpenTargetsiENSG00000164220.
PharmGKBiPA27948.

Chemistry databases

ChEMBLiCHEMBL5477.

Polymorphism and mutation databases

BioMutaiF2RL2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
PropeptideiPRO_000001275622 – 38Removed for receptor activationBy similarityAdd BLAST17
ChainiPRO_000001275739 – 374Proteinase-activated receptor 3Add BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi25N-linked (GlcNAc...)Sequence analysis1
Glycosylationi82N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi166 ↔ 245PROSITE-ProRule annotation
Glycosylationi331N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei38 – 39Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO00254.
PRIDEiO00254.

PTM databases

iPTMnetiO00254.
PhosphoSitePlusiO00254.

Miscellaneous databases

PMAP-CutDBO00254.

Expressioni

Tissue specificityi

Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.1 Publication

Gene expression databases

BgeeiENSG00000164220.
CleanExiHS_F2RL2.
GenevisibleiO00254. HS.

Interactioni

Subunit structurei

Interacts with INSC/inscuteable and probably GPSM2.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NCK1P163332EBI-1751853,EBI-389883

Protein-protein interaction databases

DIPiDIP-44337N.
IntActiO00254. 9 interactors.
MINTiMINT-3380783.
STRINGi9606.ENSP00000296641.

Structurei

3D structure databases

ProteinModelPortaliO00254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJUX. Eukaryota.
ENOG4111GWA. LUCA.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000116291.
HOVERGENiHBG105658.
InParanoidiO00254.
KOiK04235.
OMAiSAIEGWT.
OrthoDBiEOG091G0C2F.
PhylomeDBiO00254.
TreeFamiTF330775.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003943. Prot_act_rcpt_3.
IPR003912. Protea_act_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01429. PROTEASEAR3.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00254-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKALIFAAAG LLLLLPTFCQ SGMENDTNNL AKPTLPIKTF RGAPPNSFEE
60 70 80 90 100
FPFSALEGWT GATITVKIKC PEESASHLHV KNATMGYLTS SLSTKLIPAI
110 120 130 140 150
YLLVFVVGVP ANAVTLWMLF FRTRSICTTV FYTNLAIADF LFCVTLPFKI
160 170 180 190 200
AYHLNGNNWV FGEVLCRATT VIFYGNMYCS ILLLACISIN RYLAIVHPFT
210 220 230 240 250
YRGLPKHTYA LVTCGLVWAT VFLYMLPFFI LKQEYYLVQP DITTCHDVHN
260 270 280 290 300
TCESSSPFQL YYFISLAFFG FLIPFVLIIY CYAAIIRTLN AYDHRWLWYV
310 320 330 340 350
KASLLILVIF TICFAPSNII LIIHHANYYY NNTDGLYFIY LIALCLGSLN
360 370
SCLDPFLYFL MSKTRNHSTA YLTK
Length:374
Mass (Da):42,508
Last modified:July 1, 1997 - v1
Checksum:iC45C15A695DD1ABB
GO
Isoform 2 (identifier: O00254-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: Missing.

Show »
Length:352
Mass (Da):40,247
Checksum:i36F6931ABB5C5393
GO

Sequence cautioni

The sequence CAD97628 differs from that shown. Reason: Frameshift at position 374.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01284915L → S.1 PublicationCorresponds to variant rs2069649dbSNPEnsembl.1
Natural variantiVAR_012850177M → V.1 PublicationCorresponds to variant rs2069700dbSNPEnsembl.1
Natural variantiVAR_012851250N → D.1 PublicationCorresponds to variant rs2069683dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0451161 – 22Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92971 mRNA. Translation: AAC51218.1.
AK312848 mRNA. Translation: BAG35701.1.
AK298585 mRNA. Translation: BAG60775.1.
BX537386 mRNA. Translation: CAD97628.1. Frameshift.
AF374726 Genomic DNA. Translation: AAK51564.1.
AC026725 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95778.1.
BC093648 mRNA. Translation: AAH93648.1.
BC093650 mRNA. Translation: AAH93650.1.
CCDSiCCDS4031.1. [O00254-1]
CCDS58959.1. [O00254-2]
RefSeqiNP_001243495.1. NM_001256566.1. [O00254-2]
NP_004092.1. NM_004101.3. [O00254-1]
UniGeneiHs.42502.

Genome annotation databases

EnsembliENST00000296641; ENSP00000296641; ENSG00000164220. [O00254-1]
ENST00000504899; ENSP00000426703; ENSG00000164220. [O00254-2]
GeneIDi2151.
KEGGihsa:2151.
UCSCiuc003kem.4. human. [O00254-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

SeattleSNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92971 mRNA. Translation: AAC51218.1.
AK312848 mRNA. Translation: BAG35701.1.
AK298585 mRNA. Translation: BAG60775.1.
BX537386 mRNA. Translation: CAD97628.1. Frameshift.
AF374726 Genomic DNA. Translation: AAK51564.1.
AC026725 Genomic DNA. No translation available.
CH471084 Genomic DNA. Translation: EAW95778.1.
BC093648 mRNA. Translation: AAH93648.1.
BC093650 mRNA. Translation: AAH93650.1.
CCDSiCCDS4031.1. [O00254-1]
CCDS58959.1. [O00254-2]
RefSeqiNP_001243495.1. NM_001256566.1. [O00254-2]
NP_004092.1. NM_004101.3. [O00254-1]
UniGeneiHs.42502.

3D structure databases

ProteinModelPortaliO00254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-44337N.
IntActiO00254. 9 interactors.
MINTiMINT-3380783.
STRINGi9606.ENSP00000296641.

Chemistry databases

ChEMBLiCHEMBL5477.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiO00254.
PhosphoSitePlusiO00254.

Polymorphism and mutation databases

BioMutaiF2RL2.

Proteomic databases

PaxDbiO00254.
PRIDEiO00254.

Protocols and materials databases

DNASUi2151.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000296641; ENSP00000296641; ENSG00000164220. [O00254-1]
ENST00000504899; ENSP00000426703; ENSG00000164220. [O00254-2]
GeneIDi2151.
KEGGihsa:2151.
UCSCiuc003kem.4. human. [O00254-1]

Organism-specific databases

CTDi2151.
DisGeNETi2151.
GeneCardsiF2RL2.
HGNCiHGNC:3539. F2RL2.
MIMi601919. gene.
neXtProtiNX_O00254.
OpenTargetsiENSG00000164220.
PharmGKBiPA27948.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IJUX. Eukaryota.
ENOG4111GWA. LUCA.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000116291.
HOVERGENiHBG105658.
InParanoidiO00254.
KOiK04235.
OMAiSAIEGWT.
OrthoDBiEOG091G0C2F.
PhylomeDBiO00254.
TreeFamiTF330775.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000164220-MONOMER.
ReactomeiR-HSA-375276. Peptide ligand-binding receptors.
R-HSA-416476. G alpha (q) signalling events.
R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs).
SIGNORiO00254.

Miscellaneous databases

GeneWikiiF2RL2.
GenomeRNAii2151.
PMAP-CutDBO00254.
PROiO00254.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164220.
CleanExiHS_F2RL2.
GenevisibleiO00254. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003943. Prot_act_rcpt_3.
IPR003912. Protea_act_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR01429. PROTEASEAR3.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAR3_HUMAN
AccessioniPrimary (citable) accession number: O00254
Secondary accession number(s): B2R754
, B4DQ13, Q52M68, Q7Z3W3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 153 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.