UniProtKB - O00254 (PAR3_HUMAN)
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Protein
Proteinase-activated receptor 3
Gene
F2RL2
Organism
Homo sapiens (Human)
Status
Functioni
Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.1 Publication
GO - Molecular functioni
- G-protein coupled receptor activity Source: GO_Central
- phosphatidylinositol phospholipase C activity Source: ProtInc
- thrombin-activated receptor activity Source: ProtInc
GO - Biological processi
- blood coagulation Source: ProtInc
- G-protein coupled receptor signaling pathway Source: Reactome
- platelet activation Source: Reactome
- positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: GO_Central
- positive regulation of Rho protein signal transduction Source: GO_Central
- response to wounding Source: ProtInc
Keywordsi
Molecular function | G-protein coupled receptor, Receptor, Transducer |
Biological process | Blood coagulation, Hemostasis |
Enzyme and pathway databases
Reactomei | R-HSA-375276. Peptide ligand-binding receptors. R-HSA-416476. G alpha (q) signalling events. R-HSA-456926. Thrombin signalling through proteinase activated receptors (PARs). |
SIGNORi | O00254. |
Names & Taxonomyi
Protein namesi | Recommended name: Proteinase-activated receptor 3Short name: PAR-3 Alternative name(s): Coagulation factor II receptor-like 2 Thrombin receptor-like 2 |
Gene namesi | Name:F2RL2 Synonyms:PAR3 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000164220.6. |
HGNCi | HGNC:3539. F2RL2. |
MIMi | 601919. gene. |
neXtProti | NX_O00254. |
Subcellular locationi
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 39 – 94 | ExtracellularSequence analysisAdd BLAST | 56 | |
Transmembranei | 95 – 120 | Helical; Name=1Sequence analysisAdd BLAST | 26 | |
Topological domaini | 121 – 128 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 129 – 148 | Helical; Name=2Sequence analysisAdd BLAST | 20 | |
Topological domaini | 149 – 167 | ExtracellularSequence analysisAdd BLAST | 19 | |
Transmembranei | 168 – 189 | Helical; Name=3Sequence analysisAdd BLAST | 22 | |
Topological domaini | 190 – 206 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 207 – 230 | Helical; Name=4Sequence analysisAdd BLAST | 24 | |
Topological domaini | 231 – 260 | ExtracellularSequence analysisAdd BLAST | 30 | |
Transmembranei | 261 – 280 | Helical; Name=5Sequence analysisAdd BLAST | 20 | |
Topological domaini | 281 – 297 | CytoplasmicSequence analysisAdd BLAST | 17 | |
Transmembranei | 298 – 322 | Helical; Name=6Sequence analysisAdd BLAST | 25 | |
Topological domaini | 323 – 336 | ExtracellularSequence analysisAdd BLAST | 14 | |
Transmembranei | 337 – 361 | Helical; Name=7Sequence analysisAdd BLAST | 25 | |
Topological domaini | 362 – 374 | CytoplasmicSequence analysisAdd BLAST | 13 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 39 | T → P: No proteolytic cleavage by thrombin. 1 Publication | 1 | |
Mutagenesisi | 40 | F → A: Altered signal upon thrombin cleavage. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 2151. |
OpenTargetsi | ENSG00000164220. |
PharmGKBi | PA27948. |
Chemistry databases
ChEMBLi | CHEMBL5477. |
Polymorphism and mutation databases
BioMutai | F2RL2. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
PropeptideiPRO_0000012756 | 22 – 38 | Removed for receptor activationBy similarityAdd BLAST | 17 | |
ChainiPRO_0000012757 | 39 – 374 | Proteinase-activated receptor 3Add BLAST | 336 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 25 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 82 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 166 ↔ 245 | PROSITE-ProRule annotation | ||
Glycosylationi | 331 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 38 – 39 | Cleavage; by thrombinBy similarity | 2 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | O00254. |
PRIDEi | O00254. |
PTM databases
iPTMneti | O00254. |
PhosphoSitePlusi | O00254. |
Miscellaneous databases
PMAP-CutDBi | O00254. |
Expressioni
Tissue specificityi
Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut.1 Publication
Gene expression databases
Bgeei | ENSG00000164220. |
CleanExi | HS_F2RL2. |
Genevisiblei | O00254. HS. |
Interactioni
Subunit structurei
Interacts with INSC/inscuteable and probably GPSM2.1 Publication
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
NCK1 | P16333 | 2 | EBI-1751853,EBI-389883 |
Protein-protein interaction databases
DIPi | DIP-44337N. |
IntActi | O00254. 10 interactors. |
MINTi | O00254. |
STRINGi | 9606.ENSP00000296641. |
Family & Domainsi
Sequence similaritiesi
Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410IJUX. Eukaryota. ENOG4111GWA. LUCA. |
GeneTreei | ENSGT00910000143980. |
HOGENOMi | HOG000116291. |
HOVERGENi | HBG105658. |
InParanoidi | O00254. |
KOi | K04235. |
OMAi | SAIEGWT. |
OrthoDBi | EOG091G0C2F. |
PhylomeDBi | O00254. |
TreeFami | TF330775. |
Family and domain databases
InterProi | View protein in InterPro IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR003943. Prot_act_rcpt_3. IPR003912. Protea_act_rcpt. |
Pfami | View protein in Pfam PF00001. 7tm_1. 1 hit. |
PRINTSi | PR00237. GPCRRHODOPSN. PR01428. PROTEASEAR. PR01429. PROTEASEAR3. |
PROSITEi | View protein in PROSITE PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basket
Isoform 1 (identifier: O00254-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
10 20 30 40 50
MKALIFAAAG LLLLLPTFCQ SGMENDTNNL AKPTLPIKTF RGAPPNSFEE
60 70 80 90 100
FPFSALEGWT GATITVKIKC PEESASHLHV KNATMGYLTS SLSTKLIPAI
110 120 130 140 150
YLLVFVVGVP ANAVTLWMLF FRTRSICTTV FYTNLAIADF LFCVTLPFKI
160 170 180 190 200
AYHLNGNNWV FGEVLCRATT VIFYGNMYCS ILLLACISIN RYLAIVHPFT
210 220 230 240 250
YRGLPKHTYA LVTCGLVWAT VFLYMLPFFI LKQEYYLVQP DITTCHDVHN
260 270 280 290 300
TCESSSPFQL YYFISLAFFG FLIPFVLIIY CYAAIIRTLN AYDHRWLWYV
310 320 330 340 350
KASLLILVIF TICFAPSNII LIIHHANYYY NNTDGLYFIY LIALCLGSLN
360 370
SCLDPFLYFL MSKTRNHSTA YLTK
Sequence cautioni
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_012849 | 15 | L → S1 PublicationCorresponds to variant dbSNP:rs2069649Ensembl. | 1 | |
Natural variantiVAR_012850 | 177 | M → V1 PublicationCorresponds to variant dbSNP:rs2069700Ensembl. | 1 | |
Natural variantiVAR_012851 | 250 | N → D1 PublicationCorresponds to variant dbSNP:rs2069683Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045116 | 1 – 22 | Missing in isoform 2. 1 PublicationAdd BLAST | 22 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U92971 mRNA. Translation: AAC51218.1. AK312848 mRNA. Translation: BAG35701.1. AK298585 mRNA. Translation: BAG60775.1. BX537386 mRNA. Translation: CAD97628.1. Frameshift. AF374726 Genomic DNA. Translation: AAK51564.1. AC026725 Genomic DNA. No translation available. CH471084 Genomic DNA. Translation: EAW95778.1. BC093648 mRNA. Translation: AAH93648.1. BC093650 mRNA. Translation: AAH93650.1. |
CCDSi | CCDS4031.1. [O00254-1] CCDS58959.1. [O00254-2] |
RefSeqi | NP_001243495.1. NM_001256566.1. [O00254-2] NP_004092.1. NM_004101.3. [O00254-1] |
UniGenei | Hs.42502. |
Genome annotation databases
Ensembli | ENST00000296641; ENSP00000296641; ENSG00000164220. [O00254-1] ENST00000504899; ENSP00000426703; ENSG00000164220. [O00254-2] |
GeneIDi | 2151. |
KEGGi | hsa:2151. |
UCSCi | uc003kem.4. human. [O00254-1] |
Keywords - Coding sequence diversityi
Alternative splicing, PolymorphismSimilar proteinsi
Entry informationi
Entry namei | PAR3_HUMAN | |
Accessioni | O00254Primary (citable) accession number: O00254 Secondary accession number(s): B2R754 Q7Z3W3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 1, 1997 | |
Last modified: | March 28, 2018 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |