Reviewed, UniProtKB/Swiss-Prot O00232 (PSD12_HUMAN)

Names and origin

Protein names

Recommended name:
    26S proteasome non-ATPase regulatory subunit 12
Alternative name(s):
    26S proteasome regulatory subunit p55

Gene names

Name:

PSMD12

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins.
Subunit structure	Component of the PA700 complex.
Sequence similarities	Belongs to the proteasome subunit p55 family. Contains 1 PCI domain.

Ontologies

Keywords
Cellular component	Proteasome
Coding sequence diversity	Polymorphism
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from Experiment. Source: Reactome negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle Inferred from Experiment. Source: Reactome
Cellular component	cytosol Inferred from electronic annotation. Source: UniProtKB-KW proteasome regulatory particle Ref.1 Traceable author statement. Source: ProtInc
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
PAAF1	Q9BRP4	1	EBI-359733,EBI-1056358
PSMD10	O75832	1	EBI-359733,EBI-752185
PSMD6	Q15008	1	EBI-359733,EBI-359701

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.4
	Chain	2 – 456	455	26S proteasome non-ATPase regulatory subunit 12		PRO_0000173861
Regions
	Domain	237 – 417	181	PCI
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.4
Natural variations
	Natural variant	358	1	V → A: dbSNP rs2230680.		VAR_051558
Experimental info
	Sequence conflict	300	1	P → S in BAD96399. Ref.2
	Sequence conflict	398	1	V → D in BAD96399. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O00232-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 97D0BDBDB0C96195	FASTA	456	52,904
	10 20 30 40 50 60 MADGGSERAD GRIVKMEVDY SATVDQRLPE CAKLAKEGRL QEVIETLLSL EKQTRTASDM 70 80 90 100 110 120 VSTSRILVAV VKMCYEAKEW DLLNENIMLL SKRRSQLKQA VAKMVQQCCT YVEEITDLPI 130 140 150 160 170 180 KLRLIDTLRM VTEGKIYVEI ERARLTKTLA TIKEQNGDVK EAASILQELQ VETYGSMEKK 190 200 210 220 230 240 ERVEFILEQM RLCLAVKDYI RTQIISKKIN TKFFQEENTE KLKLKYYNLM IQLDQHEGSY 250 260 270 280 290 300 LSICKHYRAI YDTPCIQAES EKWQQALKSV VLYVILAPFD NEQSDLVHRI SGDKKLEEIP 310 320 330 340 350 360 KYKDLLKLFT TMELMRWSTL VEDYGMELRK GSLESPATDV FGSTEEGEKR WKDLKNRVVE 370 380 390 400 410 420 HNIRIMAKYY TRITMKRMAQ LLDLSVDESE AFLSNLVVNK TIFAKVDRLA GIINFQRPKD 430 440 450 PNNLLNDWSQ KLNSLMSLVN KTTHLIAKEE MIHNLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning and functional analysis of p44.5 and p55, two regulatory subunits of the 26S proteasome." Saito A., Watanabe T.K., Shimada Y., Fujiwara T., Slaughter C.A., DeMartino G.N., Tanahashi N., Tanaka K. Gene 203:241-250(1997) [PubMed: 9426256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Muscle and Testis.
[4]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-12, ACETYLATION AT ALA-2. Tissue: Platelet.
[5]	"Mass spectrometric characterization of the affinity-purified human 26S proteasome complex." Wang X., Chen C.-F., Baker P.R., Chen P.-L., Kaiser P., Huang L. Biochemistry 46:3553-3565(2007) [PubMed: 17323924] [Abstract] Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB003103 mRNA. Translation: BAA19749.1. AK222679 mRNA. Translation: BAD96399.1. BC019062 mRNA. Translation: AAH19062.1. BC065826 mRNA. Translation: AAH65826.1. Different initiation.
IPI	IPI00185374.
PIR	JC6523. PC6501.
RefSeq	NP_002807.1.
UniGene	Hs.646575
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:27549N.
IntAct	O00232. 9 interactions.
PTM databases
PhosphoSite	O00232.
Proteomic databases
PeptideAtlas	O00232.
PRIDE	O00232.
Genome annotation databases
Ensembl	ENSG00000197170. Homo sapiens. [Contig view]
GeneID	5718.
KEGG	hsa:5718.
UCSC	uc002jfy.1. human.
Organism-specific databases
GeneCards	GC17M062764.
H-InvDB	HIX0014093.
HGNC	HGNC:9557. PSMD12.
MIM	604450. gene.
PharmGKB	PA33903.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	O00232.
HOVERGEN	O00232.
OMA	O00232. WDALNEN.
Enzyme and pathway databases
Reactome	REACT_11045. Signaling by Wnt. REACT_13. Metabolism of amino acids. REACT_13635. Regulation of activated PAK-2p34 by proteasome mediated degradation. REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication. REACT_578. Apoptosis. REACT_6185. HIV Infection. REACT_6850. Cdc20:Phospho-APC/C mediated degradation of Cyclin A. REACT_9035. APC/C:Cdh1-mediated degradation of Skp2.
Gene expression databases
ArrayExpress	O00232.
Bgee	O00232.
CleanEx	HS_PSMD12.
GermOnline	ENSG00000197170. Homo sapiens.
Family and domain databases
InterPro	IPR000717. PCI. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01399. PCI. 1 hit. [Graphical view]
SMART	SM00088. PINT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	22216.
SOURCE	Search...

Entry information

Entry name

PSD12_HUMAN

Accession

Primary (citable) accession number: O00232
Secondary accession number(s): Q53HA2, Q6P053

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2002
Last sequence update:	January 23, 2007
Last modified:	July 7, 2009
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)