O00221 (IKBE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NF-kappa-B inhibitor epsilon
Short name=NF-kappa-BIE

Alternative name(s):
I-kappa-B-epsilon
Short name=IkB-E
Short name=IkB-epsilon
Short name=IkappaBepsilon

Gene names

Name:	NFKBIE
Synonyms:	IKBE

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes. Ref.4
Subunit structure	Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit. Ref.4
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.
Post-translational modification	Serine phosphorylated; followed by proteasome-dependent degradation.
Sequence similarities	Belongs to the NF-kappa-B inhibitor family. Contains 6 ANK repeats.
Sequence caution	The sequence AAC51216.1 differs from that shown. Reason: Frameshift at positions 339 and 351.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Polymorphism
Domain	ANK repeat Repeat
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	D-serine transport Inferred from electronic annotation. Source: Compara cytoplasmic sequestering of transcription factor Traceable author statement Ref.1. Source: ProtInc
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara cytosol Traceable author statement. Source: Reactome perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	NF-kappa-B inhibitor epsilon		PRO_0000067007
Regions
	Repeat	258 – 291	34	ANK 1
	Repeat	293 – 322	30	ANK 2
	Repeat	326 – 355	30	ANK 3
	Repeat	369 – 398	30	ANK 4
	Repeat	403 – 432	30	ANK 5
	Repeat	436 – 465	30	ANK 6
Amino acid modifications
	Modified residue	157	1	Phosphoserine Probable
	Modified residue	161	1	Phosphoserine Probable
Natural variations
	Natural variant	95	1	H → Q. Corresponds to variant rs28362857 [ dbSNP | Ensembl ].		VAR_046631
	Natural variant	194	1	V → A. Corresponds to variant rs2233434 [ dbSNP | Ensembl ].		VAR_046632
Experimental info
	Mutagenesis	145	1	K → R: No effect. Ref.1
	Mutagenesis	157	1	S → A: No degradation. Ref.1
	Mutagenesis	161	1	S → A: No degradation. Ref.1
	Sequence conflict	63 – 64	2	PA → RP in AAC51216. Ref.1
	Sequence conflict	101	1	P → R in AAC51216. Ref.1
	Sequence conflict	233	1	A → P in AAC51216. Ref.1
	Sequence conflict	402	1	T → S in AAC51216. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O00221 [UniParc]. Last modified September 23, 2008. Version 3. Checksum: 45E726E7A0E8D478	FASTA	500	52,864
	10 20 30 40 50 60 MNQRRSESRP GNHRLQAYAE PGKGDSGGAG PLSGSARRGR GGGGAIRVRR PCWSGGAGRG 70 80 90 100 110 120 GGPAWAVRLP TVTAGWTWPA LRTLSSLRAG PSEPHSPGRR PPRAGRPLCQ ADPQPGKAAR 130 140 150 160 170 180 RSLEPDPAQT GPRPARAAGM SEARKGPDEA EESQYDSGIE SLRSLRSLPE STSAPASGPS 190 200 210 220 230 240 DGSPQPCTHP PGPVKEPQEK EDADGERADS TYGSSSLTYT LSLLGGPEAE DPAPRLPLPH 250 260 270 280 290 300 VGALSPQQLE ALTYISEDGD TLVHLAVIHE APAVLLCCLA LLPQEVLDIQ NNLYQTALHL 310 320 330 340 350 360 AVHLDQPGAV RALVLKGASR ALQDRHGDTA LHVACQRQHL ACARCLLEGR PEPGRGTSHS 370 380 390 400 410 420 LDLQLQNWQG LACLHIATLQ KNQPLMELLL RNGADIDVQE GTSGKTALHL AVETQERGLV 430 440 450 460 470 480 QFLLQAGAQV DARMLNGCTP LHLAAGRGLM GISSTLCKAG ADSLLRNVED ETPQDLTEES 490 500 LVLLPFDDLK ISGKLLLCTD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"IkappaB epsilon, a novel member of the IkappaB family, controls RelA and cRel NF-kappaB activity." Whiteside S.T., Epinat J.-C., Rice N.R., Israel A. EMBO J. 16:1413-1426(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF LYS-145; SER-157 AND SER-161. Tissue: Fetal brain.
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"A new member of the IkappaB protein family, IkappaB epsilon, inhibits RelA (p65)-mediated NF-kappaB transcription." Li Z., Nabel G.J. Mol. Cell. Biol. 17:6184-6190(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 140-500, FUNCTION, INTERACTION WITH RELA; REL; NFKB1 AND NFKB2. Tissue: B-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U91616 mRNA. Translation: AAC51216.1. Frameshift. AL139392 Genomic DNA. Translation: CAI20103.1. CH471081 Genomic DNA. Translation: EAX04262.1.
IPI	IPI00414656.
RefSeq	NP_004547.2. NM_004556.2.
UniGene	Hs.458276.
3D structure databases
ProteinModelPortal	O00221.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-27533N.
IntAct	O00221. 6 interactions.
MINT	MINT-1134068.
STRING	9606.ENSP00000275015.
PTM databases
PhosphoSite	O00221.
Proteomic databases
PaxDb	O00221.
PRIDE	O00221.
Protocols and materials databases
DNASU	4794.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000275015; ENSP00000275015; ENSG00000146232.
GeneID	4794.
KEGG	hsa:4794.
UCSC	uc003oxe.1. human.
Organism-specific databases
CTD	4794.
GeneCards	GC06M044226.
H-InvDB	HIX0200879.
HGNC	HGNC:7799. NFKBIE.
HPA	CAB010272. HPA002692. HPA005941.
MIM	604548. gene.
neXtProt	NX_O00221.
PharmGKB	PA31603.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0666.
HOGENOM	HOG000059576.
HOVERGEN	HBG108320.
InParanoid	O00221.
KO	K05872.
OMA	DIDVQEG.
OrthoDB	EOG4PRSR2.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	O00221.
Bgee	O00221.
CleanEx	HS_NFKBIE.
Genevestigator	O00221.
GermOnline	ENSG00000146232. Homo sapiens.
Family and domain databases
Gene3D	1.25.40.20. 1 hit.
InterPro	IPR002110. Ankyrin_rpt. IPR020683. Ankyrin_rpt-contain_dom. [Graphical view]
Pfam	PF00023. Ank. 5 hits. [Graphical view]
PRINTS	PR01415. ANKYRIN.
SMART	SM00248. ANK. 6 hits. [Graphical view]
SUPFAM	SSF48403. ANK. 1 hit.
PROSITE	PS50297. ANK_REP_REGION. 1 hit. PS50088. ANK_REPEAT. 3 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	O00221.
ChEMBL	CHEMBL3407.
GenomeRNAi	4794.
NextBio	18476.
SOURCE	Search...

Entry information

Entry name

IKBE_HUMAN

Accession

Primary (citable) accession number: O00221
Secondary accession number(s): Q5T9V9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 122 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.