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Protein

NF-kappa-B inhibitor epsilon

Gene

NFKBIE

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. Inhibits DNA-binding of NF-kappa-B p50-p65 and p50-c-Rel complexes.1 Publication

GO - Biological processi

  • cytoplasmic sequestering of transcription factor Source: ProtInc
  • D-serine transport Source: Ensembl
Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000146232-MONOMER.
ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
SIGNORiO00221.

Names & Taxonomyi

Protein namesi
Recommended name:
NF-kappa-B inhibitor epsilon
Short name:
NF-kappa-BIE
Alternative name(s):
I-kappa-B-epsilon
Short name:
IkB-E
Short name:
IkB-epsilon
Short name:
IkappaBepsilon
Gene namesi
Name:NFKBIE
Synonyms:IKBE
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:7799. NFKBIE.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • cytosol Source: Reactome
  • Golgi apparatus Source: Ensembl
  • perinuclear region of cytoplasm Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi145K → R: No effect. 1 Publication1
Mutagenesisi157S → A: No degradation. 1 Publication1
Mutagenesisi161S → A: No degradation. 1 Publication1

Organism-specific databases

DisGeNETi4794.
OpenTargetsiENSG00000146232.
PharmGKBiPA31603.

Polymorphism and mutation databases

BioMutaiNFKBIE.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000670071 – 500NF-kappa-B inhibitor epsilonAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineCombined sources1
Modified residuei161PhosphoserineCurated1
Modified residuei183PhosphoserineCombined sources1

Post-translational modificationi

Serine phosphorylated; followed by proteasome-dependent degradation.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO00221.
MaxQBiO00221.
PaxDbiO00221.
PeptideAtlasiO00221.
PRIDEiO00221.

PTM databases

iPTMnetiO00221.
PhosphoSitePlusiO00221.

Expressioni

Tissue specificityi

Highly expressed in spleen, testis and lung, followed by kidney, pancreas, heart, placenta and brain. Also expressed in granulocytes and macrophages.

Gene expression databases

BgeeiENSG00000146232.
CleanExiHS_NFKBIE.
ExpressionAtlasiO00221. baseline and differential.
GenevisibleiO00221. HS.

Organism-specific databases

HPAiCAB010272.
HPA002692.
HPA005941.

Interactioni

Subunit structurei

Interacts with RELA, REL, NFKB1 nuclear factor NF-kappa-B p50 subunit and NFKB2 nuclear factor NF-kappa-B p52 subunit.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ANKHD1Q8IWZ32EBI-355098,EBI-359558
PPP6R1Q9UPN72EBI-355098,EBI-359745
PPP6R2O751702EBI-355098,EBI-359739

Protein-protein interaction databases

BioGridi110861. 22 interactors.
DIPiDIP-27533N.
IntActiO00221. 21 interactors.
MINTiMINT-1134068.
STRINGi9606.ENSP00000275015.

Structurei

3D structure databases

ProteinModelPortaliO00221.
SMRiO00221.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati258 – 291ANK 1Add BLAST34
Repeati293 – 322ANK 2Add BLAST30
Repeati326 – 355ANK 3Add BLAST30
Repeati369 – 398ANK 4Add BLAST30
Repeati403 – 432ANK 5Add BLAST30
Repeati436 – 465ANK 6Add BLAST30

Sequence similaritiesi

Belongs to the NF-kappa-B inhibitor family.Curated
Contains 6 ANK repeats.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00550000074527.
HOGENOMiHOG000059576.
HOVERGENiHBG108320.
InParanoidiO00221.
KOiK05872.
OMAiRNRPLME.
OrthoDBiEOG091G092R.
PhylomeDBiO00221.
TreeFamiTF320166.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O00221-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQRRSESRP GNHRLQAYAE PGKGDSGGAG PLSGSARRGR GGGGAIRVRR
60 70 80 90 100
PCWSGGAGRG GGPAWAVRLP TVTAGWTWPA LRTLSSLRAG PSEPHSPGRR
110 120 130 140 150
PPRAGRPLCQ ADPQPGKAAR RSLEPDPAQT GPRPARAAGM SEARKGPDEA
160 170 180 190 200
EESQYDSGIE SLRSLRSLPE STSAPASGPS DGSPQPCTHP PGPVKEPQEK
210 220 230 240 250
EDADGERADS TYGSSSLTYT LSLLGGPEAE DPAPRLPLPH VGALSPQQLE
260 270 280 290 300
ALTYISEDGD TLVHLAVIHE APAVLLCCLA LLPQEVLDIQ NNLYQTALHL
310 320 330 340 350
AVHLDQPGAV RALVLKGASR ALQDRHGDTA LHVACQRQHL ACARCLLEGR
360 370 380 390 400
PEPGRGTSHS LDLQLQNWQG LACLHIATLQ KNQPLMELLL RNGADIDVQE
410 420 430 440 450
GTSGKTALHL AVETQERGLV QFLLQAGAQV DARMLNGCTP LHLAAGRGLM
460 470 480 490 500
GISSTLCKAG ADSLLRNVED ETPQDLTEES LVLLPFDDLK ISGKLLLCTD
Length:500
Mass (Da):52,864
Last modified:September 23, 2008 - v3
Checksum:i45E726E7A0E8D478
GO

Sequence cautioni

The sequence AAC51216 differs from that shown. Reason: Frameshift at positions 339 and 351.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63 – 64PA → RP in AAC51216 (PubMed:9135156).Curated2
Sequence conflicti101P → R in AAC51216 (PubMed:9135156).Curated1
Sequence conflicti233A → P in AAC51216 (PubMed:9135156).Curated1
Sequence conflicti402T → S in AAC51216 (PubMed:9135156).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04663195H → Q.Corresponds to variant rs28362857dbSNPEnsembl.1
Natural variantiVAR_046632194V → A.Corresponds to variant rs2233434dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91616 mRNA. Translation: AAC51216.1. Frameshift.
AL139392 Genomic DNA. Translation: CAI20103.1.
CH471081 Genomic DNA. Translation: EAX04262.1.
CCDSiCCDS34463.1.
RefSeqiNP_004547.2. NM_004556.2.
UniGeneiHs.458276.

Genome annotation databases

EnsembliENST00000275015; ENSP00000275015; ENSG00000146232.
GeneIDi4794.
KEGGihsa:4794.
UCSCiuc003oxe.1. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U91616 mRNA. Translation: AAC51216.1. Frameshift.
AL139392 Genomic DNA. Translation: CAI20103.1.
CH471081 Genomic DNA. Translation: EAX04262.1.
CCDSiCCDS34463.1.
RefSeqiNP_004547.2. NM_004556.2.
UniGeneiHs.458276.

3D structure databases

ProteinModelPortaliO00221.
SMRiO00221.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110861. 22 interactors.
DIPiDIP-27533N.
IntActiO00221. 21 interactors.
MINTiMINT-1134068.
STRINGi9606.ENSP00000275015.

PTM databases

iPTMnetiO00221.
PhosphoSitePlusiO00221.

Polymorphism and mutation databases

BioMutaiNFKBIE.

Proteomic databases

EPDiO00221.
MaxQBiO00221.
PaxDbiO00221.
PeptideAtlasiO00221.
PRIDEiO00221.

Protocols and materials databases

DNASUi4794.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000275015; ENSP00000275015; ENSG00000146232.
GeneIDi4794.
KEGGihsa:4794.
UCSCiuc003oxe.1. human.

Organism-specific databases

CTDi4794.
DisGeNETi4794.
GeneCardsiNFKBIE.
H-InvDBHIX0200879.
HGNCiHGNC:7799. NFKBIE.
HPAiCAB010272.
HPA002692.
HPA005941.
MIMi604548. gene.
neXtProtiNX_O00221.
OpenTargetsiENSG00000146232.
PharmGKBiPA31603.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00550000074527.
HOGENOMiHOG000059576.
HOVERGENiHBG108320.
InParanoidiO00221.
KOiK05872.
OMAiRNRPLME.
OrthoDBiEOG091G092R.
PhylomeDBiO00221.
TreeFamiTF320166.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000146232-MONOMER.
ReactomeiR-HSA-1169091. Activation of NF-kappaB in B cells.
SIGNORiO00221.

Miscellaneous databases

GeneWikiiNFKBIE.
GenomeRNAii4794.
PROiO00221.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000146232.
CleanExiHS_NFKBIE.
ExpressionAtlasiO00221. baseline and differential.
GenevisibleiO00221. HS.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
[Graphical view]
PfamiPF12796. Ank_2. 2 hits.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 6 hits.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIKBE_HUMAN
AccessioniPrimary (citable) accession number: O00221
Secondary accession number(s): Q5T9V9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: September 23, 2008
Last modified: November 30, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.