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Protein

Hyaluronan synthase 3

Gene

HAS3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the addition of GlcNAc or GlcUA monosaccharides to the nascent hyaluronan polymer. Therefore, it is essential to hyaluronan synthesis a major component of most extracellular matrices that has a structural role in tissues architectures and regulates cell adhesion, migration and differentiation. This is one of the isozymes catalyzing that reaction (By similarity).By similarity

Catalytic activityi

UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan) = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-(nascent hyaluronan).
UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan) = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-(nascent hyaluronan).

Cofactori

Pathway:ihyaluronan biosynthesis

This protein is involved in the pathway hyaluronan biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway hyaluronan biosynthesis and in Glycan biosynthesis.

GO - Molecular functioni

  • hyaluronan synthase activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.1.212. 2681.
ReactomeiREACT_121301. Hyaluronan biosynthesis and export.
UniPathwayiUPA00341.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronan synthase 3 (EC:2.4.1.212)
Alternative name(s):
Hyaluronate synthase 3
Hyaluronic acid synthase 3
Short name:
HA synthase 3
Gene namesi
Name:HAS3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:4820. HAS3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei16 – 3621Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini37 – 448ExtracellularSequence Analysis
Transmembranei45 – 6521Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini66 – 377312CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei378 – 39821Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini399 – 40810ExtracellularSequence Analysis
Transmembranei409 – 42921Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini430 – 44011CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei441 – 46121Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini462 – 47312ExtracellularSequence AnalysisAdd
BLAST
Transmembranei474 – 49421Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini495 – 51521CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei516 – 53621Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini537 – 55317ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • hyaluranon cable Source: UniProtKB
  • integral component of membrane Source: ProtInc
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29196.

Polymorphism and mutation databases

BioMutaiHAS3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 553553Hyaluronan synthase 3PRO_0000197178Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi462 – 4621N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO00219.
PaxDbiO00219.
PRIDEiO00219.

PTM databases

PhosphoSiteiO00219.

Expressioni

Gene expression databases

BgeeiO00219.
CleanExiHS_HAS3.
ExpressionAtlasiO00219. baseline and differential.
GenevisibleiO00219. HS.

Organism-specific databases

HPAiCAB033850.
HPA031554.

Interactioni

Protein-protein interaction databases

BioGridi109288. 2 interactions.
STRINGi9606.ENSP00000304440.

Structurei

3D structure databases

ProteinModelPortaliO00219.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NodC/HAS family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000010337.
HOGENOMiHOG000112847.
HOVERGENiHBG000189.
InParanoidiO00219.
KOiK00752.
OMAiRGRLWNI.
OrthoDBiEOG77WWBZ.
PhylomeDBiO00219.
TreeFamiTF332506.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR026108. HA_synthase_3.
IPR026107. HAS/NodC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF6. PTHR22913:SF6. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O00219-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPVQLTTALR VVGTSLFALA VLGGILAAYV TGYQFIHTEK HYLSFGLYGA
60 70 80 90 100
ILGLHLLIQS LFAFLEHRRM RRAGQALKLP SPRRGSVALC IAAYQEDPDY
110 120 130 140 150
LRKCLRSAQR ISFPDLKVVM VVDGNRQEDA YMLDIFHEVL GGTEQAGFFV
160 170 180 190 200
WRSNFHEAGE GETEASLQEG MDRVRDVVRA STFSCIMQKW GGKREVMYTA
210 220 230 240 250
FKALGDSVDY IQVCDSDTVL DPACTIEMLR VLEEDPQVGG VGGDVQILNK
260 270 280 290 300
YDSWISFLSS VRYWMAFNVE RACQSYFGCV QCISGPLGMY RNSLLQQFLE
310 320 330 340 350
DWYHQKFLGS KCSFGDDRHL TNRVLSLGYR TKYTARSKCL TETPTKYLRW
360 370 380 390 400
LNQQTRWSKS YFREWLYNSL WFHKHHLWMT YESVVTGFFP FFLIATVIQL
410 420 430 440 450
FYRGRIWNIL LFLLTVQLVG IIKATYACFL RGNAEMIFMS LYSLLYMSSL
460 470 480 490 500
LPAKIFAIAT INKSGWGTSG RKTIVVNFIG LIPVSIWVAV LLGGLAYTAY
510 520 530 540 550
CQDLFSETEL AFLVSGAILY GCYWVALLML YLAIIARRCG KKPEQYSLAF

AEV
Length:553
Mass (Da):62,998
Last modified:September 23, 2008 - v3
Checksum:i82F3B0C932EE9EA3
GO
Isoform 2 (identifier: O00219-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     247-553: ILNKYDSWIS...EQYSLAFAEV → PPGKGMAVEDDQVQAAQVRATEAWSVHQRHVSREQ

Note: No experimental confirmation available.
Show »
Length:281
Mass (Da):31,200
Checksum:iBD9AA4E19847764B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti493 – 4931G → E in AAF36984 (Ref. 1) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti173 – 1731R → H.
Corresponds to variant rs2232229 [ dbSNP | Ensembl ].
VAR_049317

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei247 – 553307ILNKY…AFAEV → PPGKGMAVEDDQVQAAQVRA TEAWSVHQRHVSREQ in isoform 2. 1 PublicationVSP_042022Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232772 mRNA. Translation: AAF36984.1.
AK291400 mRNA. Translation: BAF84089.1.
AC009027 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83247.1.
CH471092 Genomic DNA. Translation: EAW83248.1.
BC021853 mRNA. Translation: AAH21853.1.
U86409 Genomic DNA. Translation: AAC51209.1.
CCDSiCCDS10870.1. [O00219-2]
CCDS10871.1. [O00219-1]
RefSeqiNP_001186209.1. NM_001199280.1. [O00219-1]
NP_005320.2. NM_005329.2. [O00219-1]
NP_619515.1. NM_138612.2. [O00219-2]
XP_005255978.1. XM_005255921.1. [O00219-1]
XP_011521363.1. XM_011523061.1. [O00219-1]
UniGeneiHs.592069.

Genome annotation databases

EnsembliENST00000219322; ENSP00000219322; ENSG00000103044. [O00219-2]
ENST00000306560; ENSP00000304440; ENSG00000103044.
ENST00000569188; ENSP00000454731; ENSG00000103044.
GeneIDi3038.
KEGGihsa:3038.
UCSCiuc002ewk.3. human. [O00219-2]
uc002ewl.3. human. [O00219-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF232772 mRNA. Translation: AAF36984.1.
AK291400 mRNA. Translation: BAF84089.1.
AC009027 Genomic DNA. No translation available.
CH471092 Genomic DNA. Translation: EAW83247.1.
CH471092 Genomic DNA. Translation: EAW83248.1.
BC021853 mRNA. Translation: AAH21853.1.
U86409 Genomic DNA. Translation: AAC51209.1.
CCDSiCCDS10870.1. [O00219-2]
CCDS10871.1. [O00219-1]
RefSeqiNP_001186209.1. NM_001199280.1. [O00219-1]
NP_005320.2. NM_005329.2. [O00219-1]
NP_619515.1. NM_138612.2. [O00219-2]
XP_005255978.1. XM_005255921.1. [O00219-1]
XP_011521363.1. XM_011523061.1. [O00219-1]
UniGeneiHs.592069.

3D structure databases

ProteinModelPortaliO00219.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109288. 2 interactions.
STRINGi9606.ENSP00000304440.

Protein family/group databases

CAZyiGT2. Glycosyltransferase Family 2.

PTM databases

PhosphoSiteiO00219.

Polymorphism and mutation databases

BioMutaiHAS3.

Proteomic databases

MaxQBiO00219.
PaxDbiO00219.
PRIDEiO00219.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000219322; ENSP00000219322; ENSG00000103044. [O00219-2]
ENST00000306560; ENSP00000304440; ENSG00000103044.
ENST00000569188; ENSP00000454731; ENSG00000103044.
GeneIDi3038.
KEGGihsa:3038.
UCSCiuc002ewk.3. human. [O00219-2]
uc002ewl.3. human. [O00219-1]

Organism-specific databases

CTDi3038.
GeneCardsiGC16P069140.
HGNCiHGNC:4820. HAS3.
HPAiCAB033850.
HPA031554.
MIMi602428. gene.
neXtProtiNX_O00219.
PharmGKBiPA29196.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1215.
GeneTreeiENSGT00390000010337.
HOGENOMiHOG000112847.
HOVERGENiHBG000189.
InParanoidiO00219.
KOiK00752.
OMAiRGRLWNI.
OrthoDBiEOG77WWBZ.
PhylomeDBiO00219.
TreeFamiTF332506.

Enzyme and pathway databases

UniPathwayiUPA00341.
BRENDAi2.4.1.212. 2681.
ReactomeiREACT_121301. Hyaluronan biosynthesis and export.

Miscellaneous databases

GeneWikiiHAS3.
GenomeRNAii3038.
NextBioi12028.
PROiO00219.
SOURCEiSearch...

Gene expression databases

BgeeiO00219.
CleanExiHS_HAS3.
ExpressionAtlasiO00219. baseline and differential.
GenevisibleiO00219. HS.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR026108. HA_synthase_3.
IPR026107. HAS/NodC.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR22913. PTHR22913. 1 hit.
PTHR22913:SF6. PTHR22913:SF6. 1 hit.
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of hyaluronan synthase 3."
    Spicer A.P.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.
  3. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Prostate.
  6. "Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase."
    Spicer A.P., Olson J.S., McDonald J.A.
    J. Biol. Chem. 272:8957-8961(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 275-464.

Entry informationi

Entry nameiHYAS3_HUMAN
AccessioniPrimary (citable) accession number: O00219
Secondary accession number(s): A8K5T5, Q8WTZ0, Q9NYP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: September 23, 2008
Last modified: July 22, 2015
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.