O00214 (LEG8_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galectin-8
Short name=Gal-8

Alternative name(s):
Po66 carbohydrate-binding protein
Short name=Po66-CBP
Prostate carcinoma tumor antigen 1
Short name=PCTA-1

Gene names

Name:

LGALS8

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Lectin with a marked preference for 3'-O-sialylated and 3'-O-sulfated glycans. Ref.13
Subunit structure	Homodimer. Ref.13
Subcellular location	Cytoplasm Probable.
Tissue specificity	Ubiquitous. Selective expression by prostate carcinomas versus normal prostate and benign prostatic hypertrophy.
Domain	Contains two homologous but distinct carbohydrate-binding domains.
Sequence similarities	Contains 2 galectin domains.
Sequence caution	The sequence AAB51605.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD45402.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD45403.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD45404.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAD45404.1 differs from that shown. Reason: Probable cloning artifact. The sequence AAH15818.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended. The sequence AAH16486.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Ligand	Lectin
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	T cell costimulation Inferred from electronic annotation. Source: Compara plasma cell differentiation Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular space Traceable author statement Ref.1. Source: ProtInc
Molecular_function	carbohydrate binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O00214-1)

Also known as: I;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O00214-2)

The sequence of this isoform differs from the canonical sequence as follows:
     183-183: L → LPSNRGGDISKIAPRTVYTKSKDSTVNHTLTCTKIPPMNYVSK

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 317

317

Galectin-8

PRO_0000076943

Regions

Domain

19 – 152

134

Galectin 1

Domain

187 – 317

131

Galectin 2

Region

249 – 255

7

Beta-galactoside binding By similarity

Sites

Binding site

69

1

Carbohydrate

Binding site

79

1

Carbohydrate

Binding site

89

1

Carbohydrate

Site

59

1

Critical for binding to sialylated and sulfated oligosaccharides

Natural variations

Alternative sequence

183

1

L → LPSNRGGDISKIAPRTVYTK SKDSTVNHTLTCTKIPPMNY VSK in isoform 2.

VSP_003094

Natural variant

19

1

F → Y. Ref.3 Ref.4 Ref.8 Ref.10
Corresponds to variant rs2737713 [ dbSNP | Ensembl ].

VAR_012990

Natural variant

36

1

R → C. Ref.3 Ref.4 Ref.8 Ref.10
Corresponds to variant rs1041935 [ dbSNP | Ensembl ].

VAR_009710

Natural variant

56

1

M → V. Ref.1 Ref.3 Ref.4 Ref.8 Ref.10
Corresponds to variant rs1041937 [ dbSNP | Ensembl ].

VAR_012991

Natural variant

184

1

R → S. Ref.1 Ref.3 Ref.4 Ref.10
Corresponds to variant rs2243525 [ dbSNP | Ensembl ].

VAR_063506

Experimental info

Sequence conflict

14

1

N → S in AAL77076. Ref.8

Sequence conflict

98 – 100

3

KRE → QKEK in CAA62904. Ref.2

Sequence conflict

112

1

D → A in CAA62904. Ref.2

Sequence conflict

171

1

S → V in AAB51605. Ref.1

Sequence conflict

199

1

R → G in AAL77076. Ref.8

Sequence conflict

204

1

K → Q in AAB51605. Ref.1

Sequence conflict

225

1

D → H in AAL77076. Ref.8

Sequence conflict

259

1

F → L in AAK16736. Ref.7

Isoform 2:

Sequence conflict

220

1

M → T in AAL77076. Ref.8

Secondary structure

1

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317

Helix Strand Turn

Details...

Beta strand

4 – 6

3

Beta strand

8 – 14

7

Beta strand

17 – 22

6

Beta strand

32 – 38

7

Beta strand

44 – 53

10

Turn

56 – 59

4

Beta strand

61 – 69

9

Beta strand

71 – 73

3

Beta strand

75 – 82

8

Beta strand

90 – 92

3

Beta strand

102 – 109

8

Beta strand

111 – 118

8

Beta strand

121 – 127

7

Helix

132 – 134

3

Beta strand

137 – 143

7

Beta strand

145 – 152

8

Beta strand

185 – 190

6

Beta strand

200 – 207

8

Beta strand

213 – 220

8

Turn

221 – 224

4

Beta strand

225 – 233

9

Turn

234 – 237

4

Beta strand

238 – 244

7

Beta strand

246 – 249

4

Beta strand

256 – 258

3

Beta strand

266 – 273

8

Beta strand

275 – 282

8

Beta strand

285 – 291

7

Helix

297 – 299

3

Beta strand

302 – 317

16

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (I) [UniParc]. Last modified March 23, 2010. Version 4. Checksum: AA13116AC5C0D69A	FASTA	317	35,808
	10 20 30 40 50 60 MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA 70 80 90 100 110 120 DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG 130 140 150 160 170 180 KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT 190 200 210 220 230 240 PQLRLPFAAR LNTPMGPGRT VVVKGEVNAN AKSFNVDLLA GKSKDIALHL NPRLNIKAFV 250 260 270 280 290 300 RNSFLQESWG EEERNITSFP FSPGMYFEMI IYCDVREFKV AVNGVHSLEY KHRFKELSSI 310 DTLEINGDIH LLEVRSW
	Isoform 2 [UniParc]. Checksum: 39BED76B4115A798	FASTA	359	40,397
	10 20 30 40 50 60 MMLSLNNLQN IIYNPVIPFV GTIPDQLDPG TLIVIRGHVP SDADRFQVDL QNGSSMKPRA 70 80 90 100 110 120 DVAFHFNPRF KRAGCIVCNT LINEKWGREE ITYDTPFKRE KSFEIVIMVL KDKFQVAVNG 130 140 150 160 170 180 KHTLLYGHRI GPEKIDTLGI YGKVNIHSIG FSFSSDLQST QASSLELTEI SRENVPKSGT 190 200 210 220 230 240 PQLPSNRGGD ISKIAPRTVY TKSKDSTVNH TLTCTKIPPM NYVSKRLPFA ARLNTPMGPG 250 260 270 280 290 300 RTVVVKGEVN ANAKSFNVDL LAGKSKDIAL HLNPRLNIKA FVRNSFLQES WGEEERNITS 310 320 330 340 350 FPFSPGMYFE MIIYCDVREF KVAVNGVHSL EYKHRFKELS SIDTLEINGD IHLLEVRSW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Surface-epitope masking and expression cloning identifies the human prostate carcinoma tumor antigen gene PCTA-1 a member of the galectin gene family." Su Z.-Z., Lin J., Shen R., Fisher P.E., Goldstein N.I., Fisher P.B. Proc. Natl. Acad. Sci. U.S.A. 93:7252-7257(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS VAL-56 AND SER-184. Tissue: Prostate.
[2]	"Galectin-8: on the road from structure to function." Hadari Y.R., Eisenstein M., Zakut R., Zick Y. Trends Glycosci. Glycotechnol. 9:103-112(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Hippocampus.
[3]	"Molecular cloning of a beta-galactoside-binding lectin related to galectin-8 and identified in human lung carcinoma." Brichory F., Bidon N., Desrues B., Bourguet P., Le Pennec J.-P., Dazord L. Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, VARIANTS TYR-19; CYS-36; VAL-56 AND SER-184. Tissue: Lung carcinoma.
[4]	"Genomic organization and expression of the human galectin-8 gene." Maier C., Haeussler J., Roesch K., Moschgath E., Vogel W. Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS TYR-19; CYS-36; VAL-56 AND SER-184.
[5]	"Molecular characterization of prostate carcinoma tumor antigen-1, PCTA-1, a human galectin-8 related gene." Gopalkrishnan R.V., Roberts T., Tuli S., Kang D., Christiansen K.A., Fisher P.B. Oncogene 19:4405-4416(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"Coca (colorectal carcinoma-derived) galectin-8 variant I full-length cDNA from a human colorectal carcinoma cell line." Lahm H., Siebert H.-C., Andre S., Hoeflich A., Diehl D., Sordat B., Kaltner H., Wolf E., Gabius H.-J. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Colon carcinoma.
[7]	"Coca (Colorectal carcinoma-derived) galectin-8 variant II." Lahm H., Siebert H.-C., Andre S., Hoeflich A., Diehl D., Sordat B., Kaltner H., Wolf E., Gabius H.-J. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Colon carcinoma.
[8]	"Galectins in murine and human non-Hodgkin's lymphomas." Moisan S., Mercier J., Demers M., Belanger S.D., Alain T., Kossakowska A.E., Potworowski E.F., St-Pierre Y. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANTS TYR-19; CYS-36 AND VAL-56.
[9]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), VARIANTS TYR-19; CYS-36; VAL-56 AND SER-184. Tissue: Brain and Skin.
[11]	"Solution structure of the C-terminal Gal-bind lectin protein from human galectin-8." RIKEN structural genomics initiative (RSGI) Submitted (FEB-2008) to the PDB data bank Cited for: STRUCTURE BY NMR OF 176-317.
[12]	"Crystal structure of N-terminal domain of human galectin-8." RIKEN structural genomics initiative (RSGI) Submitted (MAY-2008) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.92 ANGSTROMS) OF 1-152 IN COMPLEX WITH LACTOSE.
[13]	"Galectin-8-N-domain recognition mechanism for sialylated and sulfated glycans." Ideo H., Matsuzaka T., Nonaka T., Seko A., Yamashita K. J. Biol. Chem. 286:11346-11355(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.33 ANGSTROMS) OF 1-154 ALONE AND IN COMPLEX WITH CARBOHYDRATES, FUNCTION, SUBUNIT.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - Glycan Binding

Galectin-8 C-terminal CRD

Functional Glycomics Gateway - Glycan Binding

Galectin-8 long isoform

Functional Glycomics Gateway - Glycan Binding

Galectin-8 long isoform

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L78132 mRNA. Translation: AAB51605.1. Different initiation.
X91790 mRNA. Translation: CAA62904.1.
AF074000 mRNA. Translation: AAD45402.1. Different initiation.
AF074001 mRNA. Translation: AAD45403.1. Different initiation.
AF074002 mRNA. Translation: AAD45404.1. Sequence problems.
AF193806, AF193805 Genomic DNA. Translation: AAF19370.1.
AF342815 mRNA. Translation: AAK16735.1.
AF342816 mRNA. Translation: AAK16736.1.
AF468213 mRNA. Translation: AAL77076.1.
AL359921 Genomic DNA. Translation: CAI13773.1.
AL359921 Genomic DNA. Translation: CAI13774.1.
BC015818 mRNA. Translation: AAH15818.1. Different initiation.
BC016486 mRNA. Translation: AAH16486.2. Different initiation.

IPI

IPI00010844.
IPI00165587.

PIR

JC6147.

RefSeq

NP_006490.3. NM_006499.4.
NP_963837.1. NM_201543.2.
NP_963838.1. NM_201544.2.
NP_963839.1. NM_201545.2.

UniGene

Hs.4082.
Hs.708114.
Hs.735982.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YRO	NMR	-	A	176-317	[»]
2YV8	X-ray	1.92	A	1-152	[»]
2YXS	X-ray	2.13	A	1-152	[»]
3AP4	X-ray	2.33	A/B/C/D	1-154	[»]
3AP5	X-ray	1.92	A	1-154	[»]
3AP6	X-ray	1.58	A/B/C/D	1-154	[»]
3AP7	X-ray	1.53	A	1-154	[»]
3AP9	X-ray	1.33	A	1-154	[»]
3APB	X-ray	1.95	A/B	1-154	[»]
3OJB	X-ray	3.01	A/B/C/D	186-315	[»]
3VKL	X-ray	2.55	A/B	1-317	[»]
3VKM	X-ray	2.98	A/B	1-317	[»]
3VKN	X-ray	1.98	A/B	1-153	[»]
3VKO	X-ray	2.08	A/B	1-153	[»]
4FQZ	X-ray	2.80	A	1-317	[»]

ProteinModelPortal

O00214.

SMR

O00214. Positions 1-317.

ModBase

Search...

Protein-protein interaction databases

IntAct

O00214. 1 interaction.

MINT

MINT-1458207.

PTM databases

PhosphoSite

O00214.

Proteomic databases

PaxDb

O00214.

PRIDE

O00214.

Protocols and materials databases

DNASU

3964.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000341872; ENSP00000342139; ENSG00000116977.
ENST00000352231; ENSP00000309576; ENSG00000116977.
ENST00000366584; ENSP00000355543; ENSG00000116977.
ENST00000450372; ENSP00000408657; ENSG00000116977.
ENST00000526589; ENSP00000435460; ENSG00000116977.
ENST00000526634; ENSP00000437040; ENSG00000116977.
ENST00000527974; ENSP00000431398; ENSG00000116977.

GeneID

3964.

KEGG

hsa:3964.

UCSC

uc001hxw.2. human.
uc001hxz.2. human.

Organism-specific databases

CTD

3964.

GeneCards

GC01P236681.

HGNC

HGNC:6569. LGALS8.

HPA

HPA030491.

MIM

606099. gene.

neXtProt

NX_O00214.

PharmGKB

PA30346.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG264430.

HOVERGEN

HBG002412.

KO

K06832.

Gene expression databases

ArrayExpress

O00214.

Bgee

O00214.

Genevestigator

O00214.

GermOnline

ENSG00000116977. Homo sapiens.

Family and domain databases

Gene3D

2.60.120.200. 2 hits.

InterPro

IPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR001079. Galectin_CRD.
[Graphical view]

Pfam

PF00337. Gal-bind_lectin. 2 hits.
[Graphical view]

SMART

SM00908. Gal-bind_lectin. 2 hits.
SM00276. GLECT. 2 hits.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 2 hits.

PROSITE

PS51304. GALECTIN. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

O00214.

ChEMBL

CHEMBL5475.

ChiTaRS

LGALS8. human.

EvolutionaryTrace

O00214.

GenomeRNAi

3964.

NextBio

15552.

SOURCE

Search...

Entry information

Entry name

LEG8_HUMAN

Accession

Primary (citable) accession number: O00214
Secondary accession number(s): O15215 , Q5T3P5, Q5T3Q4, Q8TEV1, Q96B92, Q9BXC8, Q9H584, Q9H585, Q9UEZ6, Q9UP32, Q9UP33, Q9UP34

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	March 23, 2010
Last modified:	April 3, 2013
This is version 127 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.