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Protein

Rho-related GTP-binding protein RhoD

Gene

RHOD

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi71 – 755GTPBy similarity
Nucleotide bindingi129 – 1324GTPBy similarity

GO - Molecular functioni

  • GTPase activity Source: ProtInc
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • actin filament bundle assembly Source: UniProtKB
  • focal adhesion assembly Source: UniProtKB
  • lamellipodium assembly Source: UniProtKB
  • metabolic process Source: GOC
  • positive regulation of cell adhesion Source: UniProtKB
  • positive regulation of cell migration Source: UniProtKB
  • protein targeting Source: Ensembl
  • regulation of actin cytoskeleton reorganization Source: Ensembl
  • regulation of focal adhesion assembly Source: Ensembl
  • regulation of small GTPase mediated signal transduction Source: Reactome
  • Rho protein signal transduction Source: ProtInc
  • small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
REACT_355252. RHO GTPases Activate Formins.
SignaLinkiO00212.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoD
Alternative name(s):
Rho-related protein HP1
Short name:
RhoHP1
Gene namesi
Name:RHOD
Synonyms:ARHD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:670. RHOD.

Subcellular locationi

  • Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated
  • Early endosome 1 Publication

  • Note: Colocalizes with RAB5 to early endosomes (By similarity).By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi207 – 2071C → S: Abolishes endosomal localization. 1 Publication

Organism-specific databases

PharmGKBiPA24952.

Polymorphism and mutation databases

BioMutaiRHOD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Rho-related GTP-binding protein RhoDPRO_0000198863Add
BLAST
Propeptidei208 – 2103Removed in mature formBy similarityPRO_0000223365

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei207 – 2071Cysteine methyl esterBy similarity
Lipidationi207 – 2071S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiO00212.
PaxDbiO00212.
PRIDEiO00212.

PTM databases

PhosphoSiteiO00212.

Expressioni

Tissue specificityi

Heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues.

Gene expression databases

BgeeiO00212.
CleanExiHS_RHOD.
ExpressionAtlasiO00212. baseline and differential.
GenevisibleiO00212. HS.

Organism-specific databases

HPAiHPA024394.

Interactioni

Subunit structurei

Interacts (in GTP-bound form) with DIAPH2 isoform 3, DAPK3, FILIP1 and WHAMM. Interacts with PAK7/PAK5. Interacts (independent of GTP-loaded status) with ANKFY1.5 Publications

Protein-protein interaction databases

BioGridi119010. 16 interactions.
IntActiO00212. 6 interactions.
STRINGi9606.ENSP00000308576.

Structurei

Secondary structure

1
210
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi17 – 248Combined sources
Helixi30 – 389Combined sources
Beta strandi52 – 609Combined sources
Beta strandi63 – 719Combined sources
Beta strandi89 – 979Combined sources
Helixi101 – 1099Combined sources
Helixi111 – 1188Combined sources
Beta strandi124 – 1296Combined sources
Helixi131 – 1344Combined sources
Helixi137 – 1459Combined sources
Helixi153 – 16210Combined sources
Beta strandi166 – 1705Combined sources
Turni173 – 1764Combined sources
Helixi179 – 19214Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J1LX-ray2.50A7-197[»]
ProteinModelPortaliO00212.
SMRiO00212. Positions 15-193.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00212.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 549Effector regionSequence Analysis

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiO00212.
OrthoDBiEOG74TX0S.
PhylomeDBiO00212.
TreeFamiTF331746.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00212-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAAQAAGEE APPGVRSVKV VLVGDGGCGK TSLLMVFADG AFPESYTPTV
60 70 80 90 100
FERYMVNLQV KGKPVHLHIW DTAGQDDYDR LRPLFYPDAS VLLLCFDVTS
110 120 130 140 150
PNSFDNIFNR WYPEVNHFCK KVPIIVVGCK TDLCKDKSLV NKLRRNGLEP
160 170 180 190 200
VTYHRGQEMA RSVGAVAYLE CSARLHDNVH AVFQEAAEVA LSSRGRNFWR
210
RITQGFCVVT
Length:210
Mass (Da):23,413
Last modified:July 28, 2009 - v2
Checksum:i7B8A7AE8933C78B0
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti134 – 1341C → R.3 Publications
Corresponds to variant rs4930409 [ dbSNP | Ensembl ].
VAR_058408

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85815 Genomic DNA. Translation: BAA19652.1.
AF498973 mRNA. Translation: AAM21120.1.
AP000729 Genomic DNA. No translation available.
BC001338 mRNA. Translation: AAH01338.1.
CCDSiCCDS8155.1.
RefSeqiNP_055393.1. NM_014578.3.
UniGeneiHs.15114.

Genome annotation databases

EnsembliENST00000308831; ENSP00000308576; ENSG00000173156.
GeneIDi29984.
UCSCiuc001ojv.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85815 Genomic DNA. Translation: BAA19652.1.
AF498973 mRNA. Translation: AAM21120.1.
AP000729 Genomic DNA. No translation available.
BC001338 mRNA. Translation: AAH01338.1.
CCDSiCCDS8155.1.
RefSeqiNP_055393.1. NM_014578.3.
UniGeneiHs.15114.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J1LX-ray2.50A7-197[»]
ProteinModelPortaliO00212.
SMRiO00212. Positions 15-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi119010. 16 interactions.
IntActiO00212. 6 interactions.
STRINGi9606.ENSP00000308576.

PTM databases

PhosphoSiteiO00212.

Polymorphism and mutation databases

BioMutaiRHOD.

Proteomic databases

MaxQBiO00212.
PaxDbiO00212.
PRIDEiO00212.

Protocols and materials databases

DNASUi29984.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308831; ENSP00000308576; ENSG00000173156.
GeneIDi29984.
UCSCiuc001ojv.3. human.

Organism-specific databases

CTDi29984.
GeneCardsiGC11P066824.
HGNCiHGNC:670. RHOD.
HPAiHPA024394.
MIMi605781. gene.
neXtProtiNX_O00212.
PharmGKBiPA24952.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiO00212.
OrthoDBiEOG74TX0S.
PhylomeDBiO00212.
TreeFamiTF331746.

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
REACT_355252. RHO GTPases Activate Formins.
SignaLinkiO00212.

Miscellaneous databases

ChiTaRSiRHOD. human.
EvolutionaryTraceiO00212.
GeneWikiiRhoD.
GenomeRNAii29984.
NextBioi52746.
PROiO00212.
SOURCEiSearch...

Gene expression databases

BgeeiO00212.
CleanExiHS_RHOD.
ExpressionAtlasiO00212. baseline and differential.
GenevisibleiO00212. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-134.
    Tissue: Placenta.
  2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-134.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-134.
    Tissue: Cervix.
  5. "RhoD regulates endosome dynamics through diaphanous-related formin and Src tyrosine kinase."
    Gasman S., Kalaidzidis Y., Zerial M.
    Nat. Cell Biol. 5:195-204(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DIAPH2.
  6. "Multiple Rho proteins regulate the subcellular targeting of PAK5."
    Wu X., Frost J.A.
    Biochem. Biophys. Res. Commun. 351:328-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PAK7.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "RhoD regulates cytoskeletal dynamics via the actin nucleation-promoting factor WASp homologue associated with actin Golgi membranes and microtubules."
    Gad A.K., Nehru V., Ruusala A., Aspenstrom P.
    Mol. Biol. Cell 23:4807-4819(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REGULATION OF ACTIN CYTOSKELETON REORGANIZATION, INTERACTION WITH FILIP1.
  9. "Interaction of RhoD and ZIP kinase modulates actin filament assembly and focal adhesion dynamics."
    Nehru V., Almeida F.N., Aspenstrom P.
    Biochem. Biophys. Res. Commun. 433:163-169(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REGULATION OF ACTIN CYTOSKELETON REORGANIZATION, INTERACTION WITH DAPK3, MUTAGENESIS OF CYS-207.
  10. "RhoD binds the Rab5 effector Rabankyrin-5 and has a role in trafficking of the platelet-derived growth factor receptor."
    Nehru V., Voytyuk O., Lennartsson J., Aspenstroem P.
    Traffic 14:1242-1254(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ENDOSOMAL TRAFFICKING AND RECEPTOR INTERNALIZATION, INTERACTION WITH ANKFY1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRHOD_HUMAN
AccessioniPrimary (citable) accession number: O00212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 28, 2009
Last modified: July 22, 2015
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.