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O00212

- RHOD_HUMAN

UniProt

O00212 - RHOD_HUMAN

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Protein

Rho-related GTP-binding protein RhoD

Gene

RHOD

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi71 – 755GTPBy similarity
Nucleotide bindingi129 – 1324GTPBy similarity

GO - Molecular functioni

  1. GTPase activity Source: ProtInc
  2. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. actin filament bundle assembly Source: UniProtKB
  2. focal adhesion assembly Source: UniProtKB
  3. GTP catabolic process Source: GOC
  4. lamellipodium assembly Source: UniProtKB
  5. positive regulation of cell adhesion Source: UniProtKB
  6. positive regulation of cell migration Source: UniProtKB
  7. regulation of small GTPase mediated signal transduction Source: Reactome
  8. Rho protein signal transduction Source: ProtInc
  9. small GTPase mediated signal transduction Source: Reactome
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_11051. Rho GTPase cycle.
SignaLinkiO00212.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoD
Alternative name(s):
Rho-related protein HP1
Short name:
RhoHP1
Gene namesi
Name:RHOD
Synonyms:ARHD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 11

Organism-specific databases

HGNCiHGNC:670. RHOD.

Subcellular locationi

Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated. Early endosome 1 Publication
Note: Colocalizes with RAB5 to early endosomes (By similarity).By similarity

GO - Cellular componenti

  1. cytosol Source: Reactome
  2. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi207 – 2071C → S: Abolishes endosomal localization. 1 Publication

Organism-specific databases

PharmGKBiPA24952.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Rho-related GTP-binding protein RhoDPRO_0000198863Add
BLAST
Propeptidei208 – 2103Removed in mature formBy similarityPRO_0000223365

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei207 – 2071Cysteine methyl esterBy similarity
Lipidationi207 – 2071S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiO00212.
PaxDbiO00212.
PRIDEiO00212.

PTM databases

PhosphoSiteiO00212.

Expressioni

Tissue specificityi

Heart, placenta, liver, skeletal muscle, and pancreas and, with weaker intensity, in several other tissues.

Gene expression databases

BgeeiO00212.
CleanExiHS_RHOD.
GenevestigatoriO00212.

Organism-specific databases

HPAiHPA024394.

Interactioni

Subunit structurei

Interacts (in GTP-bound form) with DIAPH2 isoform 3, DAPK3, FILIP1 and WHAMM. Interacts with PAK7/PAK5. Interacts (independent of GTP-loaded status) with ANKFY1.5 Publications

Protein-protein interaction databases

BioGridi119010. 17 interactions.
IntActiO00212. 6 interactions.
STRINGi9606.ENSP00000308576.

Structurei

Secondary structure

1
210
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi17 – 248Combined sources
Helixi30 – 389Combined sources
Beta strandi52 – 609Combined sources
Beta strandi63 – 719Combined sources
Beta strandi89 – 979Combined sources
Helixi101 – 1099Combined sources
Helixi111 – 1188Combined sources
Beta strandi124 – 1296Combined sources
Helixi131 – 1344Combined sources
Helixi137 – 1459Combined sources
Helixi153 – 16210Combined sources
Beta strandi166 – 1705Combined sources
Turni173 – 1764Combined sources
Helixi179 – 19214Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2J1LX-ray2.50A7-197[»]
ProteinModelPortaliO00212.
SMRiO00212. Positions 15-193.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00212.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 549Effector regionSequence Analysis

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00760000119020.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiO00212.
KOiK07530.
OMAiAGQVDYD.
OrthoDBiEOG74TX0S.
PhylomeDBiO00212.
TreeFamiTF331746.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00212-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAAQAAGEE APPGVRSVKV VLVGDGGCGK TSLLMVFADG AFPESYTPTV
60 70 80 90 100
FERYMVNLQV KGKPVHLHIW DTAGQDDYDR LRPLFYPDAS VLLLCFDVTS
110 120 130 140 150
PNSFDNIFNR WYPEVNHFCK KVPIIVVGCK TDLCKDKSLV NKLRRNGLEP
160 170 180 190 200
VTYHRGQEMA RSVGAVAYLE CSARLHDNVH AVFQEAAEVA LSSRGRNFWR
210
RITQGFCVVT
Length:210
Mass (Da):23,413
Last modified:July 28, 2009 - v2
Checksum:i7B8A7AE8933C78B0
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti134 – 1341C → R.3 Publications
Corresponds to variant rs4930409 [ dbSNP | Ensembl ].
VAR_058408

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85815 Genomic DNA. Translation: BAA19652.1.
AF498973 mRNA. Translation: AAM21120.1.
AP000729 Genomic DNA. No translation available.
BC001338 mRNA. Translation: AAH01338.1.
CCDSiCCDS8155.1.
RefSeqiNP_055393.1. NM_014578.3.
UniGeneiHs.15114.

Genome annotation databases

EnsembliENST00000308831; ENSP00000308576; ENSG00000173156.
GeneIDi29984.
KEGGihsa:29984.
UCSCiuc001ojv.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D85815 Genomic DNA. Translation: BAA19652.1 .
AF498973 mRNA. Translation: AAM21120.1 .
AP000729 Genomic DNA. No translation available.
BC001338 mRNA. Translation: AAH01338.1 .
CCDSi CCDS8155.1.
RefSeqi NP_055393.1. NM_014578.3.
UniGenei Hs.15114.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2J1L X-ray 2.50 A 7-197 [» ]
ProteinModelPortali O00212.
SMRi O00212. Positions 15-193.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 119010. 17 interactions.
IntActi O00212. 6 interactions.
STRINGi 9606.ENSP00000308576.

PTM databases

PhosphoSitei O00212.

Proteomic databases

MaxQBi O00212.
PaxDbi O00212.
PRIDEi O00212.

Protocols and materials databases

DNASUi 29984.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000308831 ; ENSP00000308576 ; ENSG00000173156 .
GeneIDi 29984.
KEGGi hsa:29984.
UCSCi uc001ojv.3. human.

Organism-specific databases

CTDi 29984.
GeneCardsi GC11P066824.
HGNCi HGNC:670. RHOD.
HPAi HPA024394.
MIMi 605781. gene.
neXtProti NX_O00212.
PharmGKBi PA24952.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00760000119020.
HOGENOMi HOG000233974.
HOVERGENi HBG009351.
InParanoidi O00212.
KOi K07530.
OMAi AGQVDYD.
OrthoDBi EOG74TX0S.
PhylomeDBi O00212.
TreeFami TF331746.

Enzyme and pathway databases

Reactomei REACT_11051. Rho GTPase cycle.
SignaLinki O00212.

Miscellaneous databases

ChiTaRSi RHOD. human.
EvolutionaryTracei O00212.
GeneWikii RhoD.
GenomeRNAii 29984.
NextBioi 52746.
PROi O00212.
SOURCEi Search...

Gene expression databases

Bgeei O00212.
CleanExi HS_RHOD.
Genevestigatori O00212.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00174. RHO. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51420. RHO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT ARG-134.
    Tissue: Placenta.
  2. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
    Puhl H.L. III, Ikeda S.R., Aronstam R.S.
    Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-134.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-134.
    Tissue: Cervix.
  5. "RhoD regulates endosome dynamics through diaphanous-related formin and Src tyrosine kinase."
    Gasman S., Kalaidzidis Y., Zerial M.
    Nat. Cell Biol. 5:195-204(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DIAPH2.
  6. "Multiple Rho proteins regulate the subcellular targeting of PAK5."
    Wu X., Frost J.A.
    Biochem. Biophys. Res. Commun. 351:328-335(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PAK7.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "RhoD regulates cytoskeletal dynamics via the actin nucleation-promoting factor WASp homologue associated with actin Golgi membranes and microtubules."
    Gad A.K., Nehru V., Ruusala A., Aspenstrom P.
    Mol. Biol. Cell 23:4807-4819(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REGULATION OF ACTIN CYTOSKELETON REORGANIZATION, INTERACTION WITH FILIP1.
  9. "Interaction of RhoD and ZIP kinase modulates actin filament assembly and focal adhesion dynamics."
    Nehru V., Almeida F.N., Aspenstrom P.
    Biochem. Biophys. Res. Commun. 433:163-169(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REGULATION OF ACTIN CYTOSKELETON REORGANIZATION, INTERACTION WITH DAPK3, MUTAGENESIS OF CYS-207.
  10. "RhoD binds the Rab5 effector Rabankyrin-5 and has a role in trafficking of the platelet-derived growth factor receptor."
    Nehru V., Voytyuk O., Lennartsson J., Aspenstroem P.
    Traffic 14:1242-1254(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ENDOSOMAL TRAFFICKING AND RECEPTOR INTERNALIZATION, INTERACTION WITH ANKFY1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRHOD_HUMAN
AccessioniPrimary (citable) accession number: O00212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 28, 2009
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3