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O00198 (HRK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Activator of apoptosis harakiri
Alternative name(s):
BH3-interacting domain-containing protein 3
Neuronal death protein DP5
Gene names
Name:HRK
Synonyms:BID3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length91 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Promotes apoptosis. Ref.1 Ref.3

Subunit structure

Interacts with BCL2 and BCL2L1. Interacts with C1QBP. Ref.1 Ref.3 Ref.4

Subcellular location

Membrane; Single-pass membrane protein. Mitochondrion Ref.3 Ref.4.

Domain

The BH3 motif is required for the induction of cell death.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BCL2L1Q07817-13EBI-701322,EBI-287195
C1QBPQ070217EBI-701322,EBI-347528

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 9191Activator of apoptosis harakiri
PRO_0000143106

Regions

Transmembrane69 – 8719Helical; Potential
Motif33 – 4715BH3

Secondary structure

..... 91
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O00198 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 22E45FA2B0B7AA00

FASTA919,884
        10         20         30         40         50         60 
MCPCPLHRGR GPPAVCACSA GRLGLRSSAA QLTAARLKAL GDELHQRTMW RRRARSRRAP 

        70         80         90 
APGALPTYWP WLCAAAQVAA LAAWLLGRRN L 

« Hide

References

« Hide 'large scale' references
[1]"Harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-X(L)."
Inohara N., Ding L., Chen S., Nunez G.
EMBO J. 16:1686-1694(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH BCL2 AND BCL2L1.
[2]"The finished DNA sequence of human chromosome 12."
Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. expand/collapse author list , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32."
Sunayama J., Ando Y., Itoh N., Tomiyama A., Sakurada K., Sugiyama A., Kang D., Tashiro F., Gotoh Y., Kuchino Y., Kitanaka C.
Cell Death Differ. 11:771-781(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH C1QBP AND BCL2L1, SUBCELLULAR LOCATION.
[4]"Intrinsic order and disorder in the BCL-2 member harakiri: insights into its proapoptotic activity."
Barrera-Vilarmau S., Obregon P., de Alba E.
PLoS ONE 6:E21413-E21413(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 22-91, SUBCELLULAR LOCATION, CIRCULAR DICHROISM, INTERACTION WITH BCL2 AND BCL2L1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U76376 mRNA. Translation: AAC34931.1.
AC083806 Genomic DNA. No translation available.
RefSeqNP_003797.1. NM_003806.2.
UniGeneHs.87247.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2L58NMR-A22-53[»]
2L5BNMR-A61-91[»]
ProteinModelPortalO00198.
SMRO00198. Positions 22-53.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114276. 6 interactions.
IntActO00198. 7 interactions.
MINTMINT-1174819.
STRING9606.ENSP00000257572.

Proteomic databases

PaxDbO00198.
PRIDEO00198.

Protocols and materials databases

DNASU8739.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000257572; ENSP00000257572; ENSG00000135116.
GeneID8739.
KEGGhsa:8739.
UCSCuc001twe.4. human.

Organism-specific databases

CTD8739.
GeneCardsGC12M117299.
HGNCHGNC:5185. HRK.
HPACAB025973.
MIM603447. gene.
neXtProtNX_O00198.
PharmGKBPA29459.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG41778.
HOGENOMHOG000112951.
HOVERGENHBG005990.
InParanoidO00198.
KOK02512.
OMASALPTYW.
OrthoDBEOG75MW04.
PhylomeDBO00198.
TreeFamTF338168.

Gene expression databases

BgeeO00198.
CleanExHS_HRK.
GenevestigatorO00198.

Family and domain databases

InterProIPR017249. Apoptosis_activator_harakiri.
IPR020728. Bcl2_BH3_motif_CS.
[Graphical view]
PfamPF15196. Harakiri. 1 hit.
[Graphical view]
PIRSFPIRSF037635. BID3. 1 hit.
PROSITEPS01259. BH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO00198.
GeneWikiHRK_(gene).
GenomeRNAi8739.
NextBio32783.
PROO00198.
SOURCESearch...

Entry information

Entry nameHRK_HUMAN
AccessionPrimary (citable) accession number: O00198
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: February 19, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 12

Human chromosome 12: entries, gene names and cross-references to MIM