O00198 (HRK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Activator of apoptosis harakiri

Alternative name(s):
BH3-interacting domain-containing protein 3
Neuronal death protein DP5

Gene names

Name:	HRK
Synonyms:	BID3

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	91 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes apoptosis. Ref.1 Ref.3
Subunit structure	Interacts with BCL2 and BCL2L1. Interacts with C1QBP. Ref.1 Ref.3 Ref.4
Subcellular location	Membrane; Single-pass membrane protein. Mitochondrion Ref.3 Ref.4.
Domain	The BH3 motif is required for the induction of cell death.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW induction of apoptosis Traceable author statement. Source: ProtInc negative regulation of survival gene product expression Traceable author statement. Source: ProtInc positive regulation of protein homooligomerization Inferred from direct assay. Source: BHF-UCL positive regulation of release of cytochrome c from mitochondria Inferred from direct assay. Source: BHF-UCL
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BCL2L1	Q07817-1	2	EBI-701322,EBI-287195

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 91

91

Activator of apoptosis harakiri

PRO_0000143106

Regions

Transmembrane

69 – 87

19

Helical; Potential

Motif

33 – 47

15

BH3

Secondary structure

1

.

.

.

91

Helix Strand Turn

Details...

Helix

28 – 51

24

Sequences

	Sequence		Length	Mass (Da)
	O00198 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 22E45FA2B0B7AA00	FASTA	91	9,884
	10 20 30 40 50 60 MCPCPLHRGR GPPAVCACSA GRLGLRSSAA QLTAARLKAL GDELHQRTMW RRRARSRRAP 70 80 90 APGALPTYWP WLCAAAQVAA LAAWLLGRRN L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-X(L)." Inohara N., Ding L., Chen S., Nunez G. EMBO J. 16:1686-1694(1997) [PubMed: 9130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH BCL2 AND BCL2L1.
[2]	"The finished DNA sequence of human chromosome 12." Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R. , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A. Nature 440:346-351(2006) [PubMed: 16541075] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32." Sunayama J., Ando Y., Itoh N., Tomiyama A., Sakurada K., Sugiyama A., Kang D., Tashiro F., Gotoh Y., Kuchino Y., Kitanaka C. Cell Death Differ. 11:771-781(2004) [PubMed: 15031724] [Abstract] Cited for: FUNCTION, INTERACTION WITH C1QBP AND BCL2L1, SUBCELLULAR LOCATION.
[4]	"Intrinsic order and disorder in the BCL-2 member harakiri: insights into its proapoptotic activity." Barrera-Vilarmau S., Obregon P., de Alba E. PLoS ONE 6:E21413-E21413(2011) [PubMed: 21731739] [Abstract] Cited for: STRUCTURE BY NMR OF 22-91, SUBCELLULAR LOCATION, CIRCULAR DICHROISM, INTERACTION WITH BCL2 AND BCL2L1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U76376 mRNA. Translation: AAC34931.1.
AC083806 Genomic DNA. No translation available.

IPI

IPI00010492.

RefSeq

NP_003797.1. NM_003806.1.

UniGene

Hs.87247.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2L58	NMR	-	A	22-53	[»]
2L5B	NMR	-	A	61-91	[»]

ProteinModelPortal

O00198.

SMR

O00198. Positions 22-53.

ModBase

Search...

Protein-protein interaction databases

IntAct

O00198. 4 interactions.

STRING

O00198.

Proteomic databases

PRIDE

O00198.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000257572; ENSP00000257572; ENSG00000135116.

GeneID

8739.

KEGG

hsa:8739.

UCSC

uc001twe.1. human.

Organism-specific databases

CTD

8739.

GeneCards

GC12M117299.

HGNC

HGNC:5185. HRK.

HPA

CAB025973.

MIM

603447. gene.

neXtProt

NX_O00198.

PharmGKB

PA29459.

GenAtlas

Search...

Phylogenomic databases

eggNOG

maNOG21716.

GeneTree

ENSGT00390000007050.

HOGENOM

HBG126486.

HOVERGEN

HBG005990.

InParanoid

O00198.

OMA

ELHRRAM.

OrthoDB

EOG4ZCT67.

PhylomeDB

O00198.

Gene expression databases

ArrayExpress

O00198.

Bgee

O00198.

CleanEx

HS_HRK.

Genevestigator

O00198.

GermOnline

ENSG00000135116. Homo sapiens.

Family and domain databases

InterPro

IPR017249. Apoptosis_activator_harakiri.
IPR020728. Bcl2_BH3_motif_CS.
[Graphical view]

KO

K02512.

PIRSF

PIRSF037635. BID3. 1 hit.

PROSITE

PS01259. BH3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

32783.

SOURCE

Search...

Entry information

Entry name

HRK_HUMAN

Accession

Primary (citable) accession number: O00198

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.