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Protein

Activator of apoptosis harakiri

Gene

HRK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes apoptosis.2 Publications

GO - Biological processi

  1. apoptotic process Source: ProtInc
  2. positive regulation of apoptotic process Source: UniProtKB
  3. positive regulation of protein homooligomerization Source: BHF-UCL
  4. positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Activator of apoptosis harakiri
Alternative name(s):
BH3-interacting domain-containing protein 3
Neuronal death protein DP5
Gene namesi
Name:HRK
Synonyms:BID3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 12

Organism-specific databases

HGNCiHGNC:5185. HRK.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei69 – 8719HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA29459.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9191Activator of apoptosis harakiriPRO_0000143106Add
BLAST

Proteomic databases

PaxDbiO00198.
PRIDEiO00198.

Expressioni

Gene expression databases

BgeeiO00198.
CleanExiHS_HRK.
GenevestigatoriO00198.

Organism-specific databases

HPAiCAB025973.

Interactioni

Subunit structurei

Interacts with BCL2 and BCL2L1. Interacts with C1QBP.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
BCL2L1Q07817-13EBI-701322,EBI-287195
C1QBPQ070217EBI-701322,EBI-347528

Protein-protein interaction databases

BioGridi114276. 6 interactions.
IntActiO00198. 7 interactions.
MINTiMINT-1174819.
STRINGi9606.ENSP00000257572.

Structurei

Secondary structure

1
91
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi28 – 5124Combined sources
Helixi69 – 8517Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L58NMR-A22-53[»]
2L5BNMR-A61-91[»]
ProteinModelPortaliO00198.
SMRiO00198. Positions 22-53.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00198.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi33 – 4715BH3Add
BLAST

Domaini

The BH3 motif is required for the induction of cell death.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG41778.
GeneTreeiENSGT00390000007050.
HOGENOMiHOG000112951.
HOVERGENiHBG005990.
InParanoidiO00198.
KOiK02512.
OMAiAVCACSS.
OrthoDBiEOG75MW04.
PhylomeDBiO00198.
TreeFamiTF338168.

Family and domain databases

InterProiIPR017249. Apoptosis_activator_harakiri.
IPR020728. Bcl2_BH3_motif_CS.
[Graphical view]
PfamiPF15196. Harakiri. 1 hit.
[Graphical view]
PIRSFiPIRSF037635. BID3. 1 hit.
PROSITEiPS01259. BH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O00198-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCPCPLHRGR GPPAVCACSA GRLGLRSSAA QLTAARLKAL GDELHQRTMW
60 70 80 90
RRRARSRRAP APGALPTYWP WLCAAAQVAA LAAWLLGRRN L
Length:91
Mass (Da):9,884
Last modified:July 1, 1997 - v1
Checksum:i22E45FA2B0B7AA00
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76376 mRNA. Translation: AAC34931.1.
AC083806 Genomic DNA. No translation available.
CCDSiCCDS9181.1.
RefSeqiNP_003797.1. NM_003806.2.
UniGeneiHs.87247.

Genome annotation databases

EnsembliENST00000257572; ENSP00000257572; ENSG00000135116.
GeneIDi8739.
KEGGihsa:8739.
UCSCiuc001twe.4. human.

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76376 mRNA. Translation: AAC34931.1.
AC083806 Genomic DNA. No translation available.
CCDSiCCDS9181.1.
RefSeqiNP_003797.1. NM_003806.2.
UniGeneiHs.87247.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2L58NMR-A22-53[»]
2L5BNMR-A61-91[»]
ProteinModelPortaliO00198.
SMRiO00198. Positions 22-53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114276. 6 interactions.
IntActiO00198. 7 interactions.
MINTiMINT-1174819.
STRINGi9606.ENSP00000257572.

Proteomic databases

PaxDbiO00198.
PRIDEiO00198.

Protocols and materials databases

DNASUi8739.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000257572; ENSP00000257572; ENSG00000135116.
GeneIDi8739.
KEGGihsa:8739.
UCSCiuc001twe.4. human.

Organism-specific databases

CTDi8739.
GeneCardsiGC12M117303.
HGNCiHGNC:5185. HRK.
HPAiCAB025973.
MIMi603447. gene.
neXtProtiNX_O00198.
PharmGKBiPA29459.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG41778.
GeneTreeiENSGT00390000007050.
HOGENOMiHOG000112951.
HOVERGENiHBG005990.
InParanoidiO00198.
KOiK02512.
OMAiAVCACSS.
OrthoDBiEOG75MW04.
PhylomeDBiO00198.
TreeFamiTF338168.

Miscellaneous databases

EvolutionaryTraceiO00198.
GeneWikiiHRK_(gene).
GenomeRNAii8739.
NextBioi32783.
PROiO00198.
SOURCEiSearch...

Gene expression databases

BgeeiO00198.
CleanExiHS_HRK.
GenevestigatoriO00198.

Family and domain databases

InterProiIPR017249. Apoptosis_activator_harakiri.
IPR020728. Bcl2_BH3_motif_CS.
[Graphical view]
PfamiPF15196. Harakiri. 1 hit.
[Graphical view]
PIRSFiPIRSF037635. BID3. 1 hit.
PROSITEiPS01259. BH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-X(L)."
    Inohara N., Ding L., Chen S., Nunez G.
    EMBO J. 16:1686-1694(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH BCL2 AND BCL2L1.
  2. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32."
    Sunayama J., Ando Y., Itoh N., Tomiyama A., Sakurada K., Sugiyama A., Kang D., Tashiro F., Gotoh Y., Kuchino Y., Kitanaka C.
    Cell Death Differ. 11:771-781(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH C1QBP AND BCL2L1, SUBCELLULAR LOCATION.
  4. "Intrinsic order and disorder in the BCL-2 member harakiri: insights into its proapoptotic activity."
    Barrera-Vilarmau S., Obregon P., de Alba E.
    PLoS ONE 6:E21413-E21413(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 22-91, SUBCELLULAR LOCATION, CIRCULAR DICHROISM, INTERACTION WITH BCL2 AND BCL2L1.

Entry informationi

Entry nameiHRK_HUMAN
AccessioniPrimary (citable) accession number: O00198
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: January 7, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.