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O00198

- HRK_HUMAN

UniProt

O00198 - HRK_HUMAN

Protein

Activator of apoptosis harakiri

Gene

HRK

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Promotes apoptosis.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. apoptotic process Source: ProtInc
    2. positive regulation of apoptotic process Source: UniProtKB
    3. positive regulation of protein homooligomerization Source: BHF-UCL
    4. positive regulation of release of cytochrome c from mitochondria Source: BHF-UCL

    Keywords - Biological processi

    Apoptosis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Activator of apoptosis harakiri
    Alternative name(s):
    BH3-interacting domain-containing protein 3
    Neuronal death protein DP5
    Gene namesi
    Name:HRK
    Synonyms:BID3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 12

    Organism-specific databases

    HGNCiHGNC:5185. HRK.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrion Source: UniProtKB

    Keywords - Cellular componenti

    Membrane, Mitochondrion

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA29459.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9191Activator of apoptosis harakiriPRO_0000143106Add
    BLAST

    Proteomic databases

    PaxDbiO00198.
    PRIDEiO00198.

    Expressioni

    Gene expression databases

    BgeeiO00198.
    CleanExiHS_HRK.
    GenevestigatoriO00198.

    Organism-specific databases

    HPAiCAB025973.

    Interactioni

    Subunit structurei

    Interacts with BCL2 and BCL2L1. Interacts with C1QBP.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BCL2L1Q07817-13EBI-701322,EBI-287195
    C1QBPQ070217EBI-701322,EBI-347528

    Protein-protein interaction databases

    BioGridi114276. 6 interactions.
    IntActiO00198. 7 interactions.
    MINTiMINT-1174819.
    STRINGi9606.ENSP00000257572.

    Structurei

    Secondary structure

    1
    91
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi28 – 5124
    Helixi69 – 8517

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2L58NMR-A22-53[»]
    2L5BNMR-A61-91[»]
    ProteinModelPortaliO00198.
    SMRiO00198. Positions 22-53.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO00198.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei69 – 8719HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi33 – 4715BH3Add
    BLAST

    Domaini

    The BH3 motif is required for the induction of cell death.

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG41778.
    HOGENOMiHOG000112951.
    HOVERGENiHBG005990.
    InParanoidiO00198.
    KOiK02512.
    OMAiHISIKRY.
    OrthoDBiEOG75MW04.
    PhylomeDBiO00198.
    TreeFamiTF338168.

    Family and domain databases

    InterProiIPR017249. Apoptosis_activator_harakiri.
    IPR020728. Bcl2_BH3_motif_CS.
    [Graphical view]
    PfamiPF15196. Harakiri. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037635. BID3. 1 hit.
    PROSITEiPS01259. BH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O00198-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCPCPLHRGR GPPAVCACSA GRLGLRSSAA QLTAARLKAL GDELHQRTMW   50
    RRRARSRRAP APGALPTYWP WLCAAAQVAA LAAWLLGRRN L 91
    Length:91
    Mass (Da):9,884
    Last modified:July 1, 1997 - v1
    Checksum:i22E45FA2B0B7AA00
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U76376 mRNA. Translation: AAC34931.1.
    AC083806 Genomic DNA. No translation available.
    CCDSiCCDS9181.1.
    RefSeqiNP_003797.1. NM_003806.2.
    UniGeneiHs.87247.

    Genome annotation databases

    EnsembliENST00000257572; ENSP00000257572; ENSG00000135116.
    GeneIDi8739.
    KEGGihsa:8739.
    UCSCiuc001twe.4. human.

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U76376 mRNA. Translation: AAC34931.1 .
    AC083806 Genomic DNA. No translation available.
    CCDSi CCDS9181.1.
    RefSeqi NP_003797.1. NM_003806.2.
    UniGenei Hs.87247.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2L58 NMR - A 22-53 [» ]
    2L5B NMR - A 61-91 [» ]
    ProteinModelPortali O00198.
    SMRi O00198. Positions 22-53.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114276. 6 interactions.
    IntActi O00198. 7 interactions.
    MINTi MINT-1174819.
    STRINGi 9606.ENSP00000257572.

    Proteomic databases

    PaxDbi O00198.
    PRIDEi O00198.

    Protocols and materials databases

    DNASUi 8739.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000257572 ; ENSP00000257572 ; ENSG00000135116 .
    GeneIDi 8739.
    KEGGi hsa:8739.
    UCSCi uc001twe.4. human.

    Organism-specific databases

    CTDi 8739.
    GeneCardsi GC12M117299.
    HGNCi HGNC:5185. HRK.
    HPAi CAB025973.
    MIMi 603447. gene.
    neXtProti NX_O00198.
    PharmGKBi PA29459.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG41778.
    HOGENOMi HOG000112951.
    HOVERGENi HBG005990.
    InParanoidi O00198.
    KOi K02512.
    OMAi HISIKRY.
    OrthoDBi EOG75MW04.
    PhylomeDBi O00198.
    TreeFami TF338168.

    Miscellaneous databases

    EvolutionaryTracei O00198.
    GeneWikii HRK_(gene).
    GenomeRNAii 8739.
    NextBioi 32783.
    PROi O00198.
    SOURCEi Search...

    Gene expression databases

    Bgeei O00198.
    CleanExi HS_HRK.
    Genevestigatori O00198.

    Family and domain databases

    InterProi IPR017249. Apoptosis_activator_harakiri.
    IPR020728. Bcl2_BH3_motif_CS.
    [Graphical view ]
    Pfami PF15196. Harakiri. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037635. BID3. 1 hit.
    PROSITEi PS01259. BH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-X(L)."
      Inohara N., Ding L., Chen S., Nunez G.
      EMBO J. 16:1686-1694(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH BCL2 AND BCL2L1.
    2. "The finished DNA sequence of human chromosome 12."
      Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
      , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
      Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Physical and functional interaction between BH3-only protein Hrk and mitochondrial pore-forming protein p32."
      Sunayama J., Ando Y., Itoh N., Tomiyama A., Sakurada K., Sugiyama A., Kang D., Tashiro F., Gotoh Y., Kuchino Y., Kitanaka C.
      Cell Death Differ. 11:771-781(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH C1QBP AND BCL2L1, SUBCELLULAR LOCATION.
    4. "Intrinsic order and disorder in the BCL-2 member harakiri: insights into its proapoptotic activity."
      Barrera-Vilarmau S., Obregon P., de Alba E.
      PLoS ONE 6:E21413-E21413(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 22-91, SUBCELLULAR LOCATION, CIRCULAR DICHROISM, INTERACTION WITH BCL2 AND BCL2L1.

    Entry informationi

    Entry nameiHRK_HUMAN
    AccessioniPrimary (citable) accession number: O00198
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: July 1, 1997
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 12
      Human chromosome 12: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references

    External Data

    Dasty 3