O00194 (RB27B_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-27B

Alternative name(s):
C25KG

Gene names

Name:

RAB27B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	218 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in targeting uroplakins to urothelial apical membranes By similarity.
Subunit structure	Interacts with SYTL2, SYTL4, MYRIP and MLPH. Interacts with RPH3A and RPH3A By similarity.
Subcellular location	Membrane; Lipid-anchor.
Tissue specificity	Expressed primarily in testis.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Disulfide bond Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	multivesicular body sorting pathway Inferred from mutant phenotype PubMed 19966785. Source: UniProtKB positive regulation of exocytosis Inferred from mutant phenotype PubMed 19966785. Source: UniProtKB protein transport Inferred from electronic annotation. Source: InterPro small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi stack Inferred from direct assay PubMed 19966785. Source: UniProtKB apical plasma membrane Inferred from electronic annotation. Source: Compara multivesicular body membrane Inferred from direct assay PubMed 19966785. Source: UniProtKB trans-Golgi network transport vesicle Inferred from direct assay PubMed 19966785. Source: UniProtKB zymogen granule membrane Inferred from electronic annotation. Source: Compara
Molecular_function	GDP binding Inferred from direct assay PubMed 20937701. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB GTPase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.9

Chain

2 – 218

217

Ras-related protein Rab-27B

PRO_0000121224

Regions

Nucleotide binding

16 – 24

9

GTP

Nucleotide binding

74 – 78

5

GTP By similarity

Nucleotide binding

133 – 136

4

GTP

Nucleotide binding

163 – 165

3

GTP

Motif

38 – 46

9

Effector region By similarity

Amino acid modifications

Modified residue

2

1

N-acetylthreonine Ref.9

Modified residue

218

1

Cysteine methyl ester By similarity

Lipidation

216

1

S-geranylgeranyl cysteine By similarity

Lipidation

218

1

S-geranylgeranyl cysteine By similarity

Disulfide bond

123 ↔ 188

Ref.11

Natural variations

Natural variant

92

1

A → T.
Corresponds to variant rs9966265 [ dbSNP | Ensembl ].

VAR_051714

Secondary structure

1

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218

Helix Strand Turn

Details...

Beta strand

7 – 16

10

Helix

22 – 30

9

Beta strand

37 – 54

18

Beta strand

65 – 77

13

Helix

78 – 89

12

Beta strand

94 – 100

7

Helix

104 – 115

12

Turn

122 – 124

3

Beta strand

127 – 133

7

Helix

138 – 140

3

Helix

145 – 154

10

Beta strand

159 – 162

4

Turn

164 – 166

3

Helix

170 – 188

19

Sequences

	Sequence		Length	Mass (Da)
	O00194 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 8ED640F0C15EDCD3	FASTA	218	24,608
	10 20 30 40 50 60 MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR VVYNAQGPNG 70 80 90 100 110 120 SSGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD LTSQQSFLNV RNWMSQLQAN 130 140 150 160 170 180 AYCENPDIVL IGNKADLPDQ REVNERQARE LADKYGIPYF ETSAATGQNV EKAVETLLDL 190 200 210 IMKRMEQCVE KTQIPDTVNG GNSGNLDGEK PPEKKCIC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of rab27a and rab27b, novel human rab proteins shared by melanocytes and platelets." Chen D., Guo J., Miki T., Tachibana M., Gahl W.A. Biochem. Mol. Med. 60:27-37(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Melanocyte.
[2]	Chen D., Gahl W.A. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 206.
[3]	"Chromosomal mapping, gene structure and characterization of the human and murine RAB27B gene." Ramalho J.S., Tolmachova T., Hume A.N., McGuigan A., Gregory-Evans C.Y., Huxley C., Seabra M.C. BMC Genet. 2:2-2(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Amygdala.
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Prostate.
[8]	"Purification, identification, and characterization of two GTP-binding proteins with molecular weights of 25,000 and 21,000 in human platelet cytosol. One is the rap1/smg21/Krev-1 protein and the other is a novel GTP-binding protein." Nagata K., Itoh H., Katada T., Takenaka K., Ui M., Kaziro Y., Nozawa Y. J. Biol. Chem. 264:17000-17005(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE. Tissue: Platelet.
[9]	Bienvenut W.V., Claeys D. Submitted (NOV-2005) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-28; 38-47; 50-64; 68-80 AND 150-184, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, MASS SPECTROMETRY. Tissue: Platelet.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"The crystal structure of human RAB27B." Structural genomics consortium (SGC) Submitted (FEB-2009) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 4-201 IN COMPLEX WITH GDP, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U57093 mRNA. Translation: AAC51194.2.
AF329499 mRNA. Translation: AAK11243.1.
AF498954 mRNA. Translation: AAM21102.1.
AK314115 mRNA. Translation: BAG36807.1.
CH471096 Genomic DNA. Translation: EAW63010.1.
BC027474 mRNA. Translation: AAH27474.1.

IPI

IPI00010491.

RefSeq

NP_004154.2. NM_004163.4.

UniGene

Hs.25318.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2F7S	X-ray	2.70	A/B	4-200	[»]

ProteinModelPortal

O00194.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48948N.

STRING

9606.ENSP00000262094.

PTM databases

PhosphoSite

O00194.

2D gel databases

OGP

O00194.

Proteomic databases

PaxDb

O00194.

PRIDE

O00194.

Protocols and materials databases

DNASU

5874.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000262094; ENSP00000262094; ENSG00000041353.

GeneID

5874.

KEGG

hsa:5874.

UCSC

uc002lfr.3. human.

Organism-specific databases

CTD

5874.

GeneCards

GC18P052495.

HGNC

HGNC:9767. RAB27B.

HPA

CAB017712.
HPA019849.

MIM

603869. gene.

neXtProt

NX_O00194.

PharmGKB

PA34118.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000233968.

HOVERGEN

HBG009351.

InParanoid

O00194.

KO

K07886.

OMA

QNVEKSV.

OrthoDB

EOG4PRSRM.

PhylomeDB

O00194.

Gene expression databases

Bgee

O00194.

CleanEx

HS_RAB27B.

Genevestigator

O00194.

GermOnline

ENSG00000041353. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O00194.

GenomeRNAi

5874.

NextBio

22822.

SOURCE

Search...

Entry information

Entry name

RB27B_HUMAN

Accession

Primary (citable) accession number: O00194
Secondary accession number(s): B2RAB0, Q9BZB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 127 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.