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O00194

- RB27B_HUMAN

UniProt

O00194 - RB27B_HUMAN

Protein

Ras-related protein Rab-27B

Gene

RAB27B

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 140 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    May be involved in targeting uroplakins to urothelial apical membranes.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi16 – 249GTP
    Nucleotide bindingi74 – 785GTPBy similarity
    Nucleotide bindingi133 – 1364GTP
    Nucleotide bindingi163 – 1653GTP

    GO - Molecular functioni

    1. GDP binding Source: UniProtKB
    2. GTPase activity Source: UniProtKB
    3. GTP binding Source: UniProtKB
    4. protein domain specific binding Source: UniProtKB

    GO - Biological processi

    1. multivesicular body sorting pathway Source: UniProtKB
    2. positive regulation of exocytosis Source: UniProtKB
    3. protein transport Source: InterPro
    4. small GTPase mediated signal transduction Source: InterPro

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ras-related protein Rab-27B
    Alternative name(s):
    C25KG
    Gene namesi
    Name:RAB27B
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 18

    Organism-specific databases

    HGNCiHGNC:9767. RAB27B.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. extracellular vesicular exosome Source: UniProt
    3. Golgi stack Source: UniProtKB
    4. multivesicular body membrane Source: UniProtKB
    5. trans-Golgi network transport vesicle Source: UniProtKB
    6. zymogen granule membrane Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA34118.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 218217Ras-related protein Rab-27BPRO_0000121224Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylthreonine1 Publication
    Disulfide bondi123 ↔ 1881 Publication
    Lipidationi216 – 2161S-geranylgeranyl cysteineBy similarity
    Modified residuei218 – 2181Cysteine methyl esterBy similarity
    Lipidationi218 – 2181S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiO00194.
    PaxDbiO00194.
    PRIDEiO00194.

    2D gel databases

    OGPiO00194.

    PTM databases

    PhosphoSiteiO00194.

    Expressioni

    Tissue specificityi

    Expressed primarily in testis.

    Gene expression databases

    ArrayExpressiO00194.
    BgeeiO00194.
    CleanExiHS_RAB27B.
    GenevestigatoriO00194.

    Organism-specific databases

    HPAiCAB017712.
    HPA019849.

    Interactioni

    Subunit structurei

    Interacts with SYTL2, SYTL4, MYRIP and MLPH. Interacts with RPH3A and RPH3A By similarity.By similarity

    Protein-protein interaction databases

    BioGridi111812. 7 interactions.
    DIPiDIP-48948N.
    STRINGi9606.ENSP00000262094.

    Structurei

    Secondary structure

    1
    218
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi7 – 1610
    Helixi22 – 309
    Beta strandi37 – 5418
    Beta strandi65 – 7713
    Helixi78 – 8912
    Beta strandi94 – 1007
    Helixi104 – 11512
    Turni122 – 1243
    Beta strandi127 – 1337
    Helixi138 – 1403
    Helixi145 – 15410
    Beta strandi159 – 1624
    Turni164 – 1663
    Helixi170 – 18819

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2F7SX-ray2.70A/B4-201[»]
    ProteinModelPortaliO00194.
    SMRiO00194. Positions 5-190.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO00194.

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi38 – 469Effector regionBy similarity

    Sequence similaritiesi

    Belongs to the small GTPase superfamily. Rab family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    HOGENOMiHOG000233968.
    HOVERGENiHBG009351.
    InParanoidiO00194.
    KOiK07886.
    OMAiSSCENAA.
    PhylomeDBiO00194.
    TreeFamiTF312895.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view]
    PfamiPF00071. Ras. 1 hit.
    [Graphical view]
    PRINTSiPR00449. RASTRNSFRMNG.
    SMARTiSM00175. RAB. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    TIGRFAMsiTIGR00231. small_GTP. 1 hit.
    PROSITEiPS51419. RAB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O00194-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDGDYDYLI KLLALGDSGV GKTTFLYRYT DNKFNPKFIT TVGIDFREKR    50
    VVYNAQGPNG SSGKAFKVHL QLWDTAGQER FRSLTTAFFR DAMGFLLMFD 100
    LTSQQSFLNV RNWMSQLQAN AYCENPDIVL IGNKADLPDQ REVNERQARE 150
    LADKYGIPYF ETSAATGQNV EKAVETLLDL IMKRMEQCVE KTQIPDTVNG 200
    GNSGNLDGEK PPEKKCIC 218
    Length:218
    Mass (Da):24,608
    Last modified:January 23, 2007 - v4
    Checksum:i8ED640F0C15EDCD3
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti92 – 921A → T.
    Corresponds to variant rs9966265 [ dbSNP | Ensembl ].
    VAR_051714

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57093 mRNA. Translation: AAC51194.2.
    AF329499 mRNA. Translation: AAK11243.1.
    AF498954 mRNA. Translation: AAM21102.1.
    AK314115 mRNA. Translation: BAG36807.1.
    CH471096 Genomic DNA. Translation: EAW63010.1.
    BC027474 mRNA. Translation: AAH27474.1.
    CCDSiCCDS11958.1.
    RefSeqiNP_004154.2. NM_004163.4.
    XP_005266790.1. XM_005266733.1.
    XP_005266791.1. XM_005266734.2.
    UniGeneiHs.25318.

    Genome annotation databases

    EnsembliENST00000262094; ENSP00000262094; ENSG00000041353.
    GeneIDi5874.
    KEGGihsa:5874.
    UCSCiuc002lfr.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57093 mRNA. Translation: AAC51194.2 .
    AF329499 mRNA. Translation: AAK11243.1 .
    AF498954 mRNA. Translation: AAM21102.1 .
    AK314115 mRNA. Translation: BAG36807.1 .
    CH471096 Genomic DNA. Translation: EAW63010.1 .
    BC027474 mRNA. Translation: AAH27474.1 .
    CCDSi CCDS11958.1.
    RefSeqi NP_004154.2. NM_004163.4.
    XP_005266790.1. XM_005266733.1.
    XP_005266791.1. XM_005266734.2.
    UniGenei Hs.25318.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2F7S X-ray 2.70 A/B 4-201 [» ]
    ProteinModelPortali O00194.
    SMRi O00194. Positions 5-190.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111812. 7 interactions.
    DIPi DIP-48948N.
    STRINGi 9606.ENSP00000262094.

    PTM databases

    PhosphoSitei O00194.

    2D gel databases

    OGPi O00194.

    Proteomic databases

    MaxQBi O00194.
    PaxDbi O00194.
    PRIDEi O00194.

    Protocols and materials databases

    DNASUi 5874.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000262094 ; ENSP00000262094 ; ENSG00000041353 .
    GeneIDi 5874.
    KEGGi hsa:5874.
    UCSCi uc002lfr.3. human.

    Organism-specific databases

    CTDi 5874.
    GeneCardsi GC18P052385.
    HGNCi HGNC:9767. RAB27B.
    HPAi CAB017712.
    HPA019849.
    MIMi 603869. gene.
    neXtProti NX_O00194.
    PharmGKBi PA34118.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1100.
    HOGENOMi HOG000233968.
    HOVERGENi HBG009351.
    InParanoidi O00194.
    KOi K07886.
    OMAi SSCENAA.
    PhylomeDBi O00194.
    TreeFami TF312895.

    Miscellaneous databases

    EvolutionaryTracei O00194.
    GenomeRNAii 5874.
    NextBioi 22822.
    PROi O00194.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O00194.
    Bgeei O00194.
    CleanExi HS_RAB27B.
    Genevestigatori O00194.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR001806. Small_GTPase.
    IPR003579. Small_GTPase_Rab_type.
    [Graphical view ]
    Pfami PF00071. Ras. 1 hit.
    [Graphical view ]
    PRINTSi PR00449. RASTRNSFRMNG.
    SMARTi SM00175. RAB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    TIGRFAMsi TIGR00231. small_GTP. 1 hit.
    PROSITEi PS51419. RAB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of rab27a and rab27b, novel human rab proteins shared by melanocytes and platelets."
      Chen D., Guo J., Miki T., Tachibana M., Gahl W.A.
      Biochem. Mol. Med. 60:27-37(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Melanocyte.
    2. Chen D., Gahl W.A.
      Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 206.
    3. "Chromosomal mapping, gene structure and characterization of the human and murine RAB27B gene."
      Ramalho J.S., Tolmachova T., Hume A.N., McGuigan A., Gregory-Evans C.Y., Huxley C., Seabra M.C.
      BMC Genet. 2:2-2(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Puhl H.L. III, Ikeda S.R., Aronstam R.S.
      Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Amygdala.
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Prostate.
    8. "Purification, identification, and characterization of two GTP-binding proteins with molecular weights of 25,000 and 21,000 in human platelet cytosol. One is the rap1/smg21/Krev-1 protein and the other is a novel GTP-binding protein."
      Nagata K., Itoh H., Katada T., Takenaka K., Ui M., Kaziro Y., Nozawa Y.
      J. Biol. Chem. 264:17000-17005(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Tissue: Platelet.
    9. Bienvenut W.V., Claeys D.
      Submitted (NOV-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 2-28; 38-47; 50-64; 68-80 AND 150-184, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT THR-2, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Platelet.
    10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "The crystal structure of human RAB27B."
      Structural genomics consortium (SGC)
      Submitted (FEB-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 4-201 IN COMPLEX WITH GDP, DISULFIDE BOND.

    Entry informationi

    Entry nameiRB27B_HUMAN
    AccessioniPrimary (citable) accession number: O00194
    Secondary accession number(s): B2RAB0, Q9BZB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 140 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 18
      Human chromosome 18: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3