Reviewed, UniProtKB/Swiss-Prot O00189 (AP4M1_HUMAN)

Names and origin

Protein names

Recommended name:
    AP-4 complex subunit mu-1
Alternative name(s):
    Adapter-related protein complex 4 mu-1 subunit
    AP-4 adapter complex mu subunit
    Mu subunit of AP-4
    Mu4-adaptin
      Short name=mu4
    Mu-adaptin-related protein 2
      Short name=mu-ARP2

Gene names

Name:	AP4M1
Synonyms:	MUARP2

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.
Subunit structure	Adapter-like complex 4 (AP-4) is an heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1). Interacts with the C-terminal domain of GRID2 By similarity.
Subcellular location	Golgi apparatus › trans-Golgi network. Membrane › coated pit. Note: Associated with the trans-Golgi network. Found in soma and dendritic shafts of neuronal cells.
Tissue specificity	Ubiquitous. Highly expressed in testis and lowly expressed in brain and lung.
Domain	Interacts specifically with tyrosine-based sorting signals.
Sequence similarities	Belongs to the adaptor complexes medium subunit family. Contains 1 MHD (mu homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Coated pit Golgi apparatus Membrane
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Traceable author statement. Source: ProtInc
Cellular component	Golgi trans cisterna Traceable author statement. Source: ProtInc clathrin adaptor complex Inferred from electronic annotation. Source: InterPro coated pit Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro transporter activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 453	453	AP-4 complex subunit mu-1		PRO_0000193787
Regions
	Domain	181 – 453	273	MHD
Amino acid modifications
	Modified residue	342	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	338	1	R → G in AAD43328. Ref.2
	Sequence conflict	345	1	Q → R in CAA69667. Ref.1
	Sequence conflict	345	1	Q → R in AAD25869. Ref.3
	Sequence conflict	400	1	L → M in AAD43328. Ref.2
	Sequence conflict	417	1	S → C in AAD43328. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O00189-1 [UniParc]. Last modified April 17, 2007. Version 2. Checksum: AE3DCA8C5AED08B7	FASTA	453	49,977
	10 20 30 40 50 60 MISQFFILSS KGDPLIYKDF RGDSGGRDVA ELFYRKLTGL PGDESPVVMH HHGRHFIHIR 70 80 90 100 110 120 HSGLYLVVTT SENVSPFSLL ELLSRLATLL GDYCGSLGEG TISRNVALVY ELLDEVLDYG 130 140 150 160 170 180 YVQTTSTEML RNFIQTEAVV SKPFSLFDLS SVGLFGAETQ QSKVAPSSAA SRPVLSSRSD 190 200 210 220 230 240 QSQKNEVFLD VVERLSVLIA SNGSLLKVDV QGEIRLKSFL PSGSEMRIGL TEEFCVGKSE 250 260 270 280 290 300 LRGYGPGIRV DEVSFHSSVN LDEFESHRIL RLQPPQGELT VMRYQLSDDL PSPLPFRLFP 310 320 330 340 350 360 SVQWDRGSGR LQVYLKLRCD LLSKSQALNV RLHLPLPRGV VSLSQELSSP EQKAELAEGA 370 380 390 400 410 420 LRWDLPRVQG GSQLSGLFQM DVPGPPGPPS HGLSTSASPL GLGPASLSFE LPRHTCSGLQ 430 440 450 VRFLRLAFRP CGNANPHKWV RHLSHSDAYV IRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of two new mu-adaptin-related proteins, mu-ARP1 and mu-ARP2." Wang X., Kilimann M.W. FEBS Lett. 402:57-61(1997) [PubMed: 9013859] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Characterization of a fourth adaptor-related protein complex." Hirst J., Bright N.A., Rous B., Robinson M.S. Mol. Biol. Cell 10:2787-2802(1999) [PubMed: 10436028] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"Signal-binding specificity of the mu4 subunit of the adaptor protein complex, AP-4." Aguilar R.C., Boehm M., Gorshkova I., Crouch R.J., Tomita K., Saito T., Ohno H., Bonifacino J.S. J. Biol. Chem. 276:13145-13152(2001) [PubMed: 11139587] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION. Tissue: Spleen.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra." Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D. J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-342, MASS SPECTROMETRY. Tissue: Epithelium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y08387 mRNA. Translation: CAA69667.1. AF155158 mRNA. Translation: AAD43328.1. AF020796 mRNA. Translation: AAD25869.1. BC018705 mRNA. Translation: AAH18705.1.
IPI	IPI00103040.
RefSeq	NP_004713.2.
UniGene	Hs.632317
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O00189.
PTM databases
PhosphoSite	O00189.
Proteomic databases
PRIDE	O00189.
Genome annotation databases
Ensembl	ENST00000359593; ENSP00000352603; ENSG00000221838; Homo sapiens. [Genome view] ENST00000421755; ENSP00000412185; ENSG00000221838; Homo sapiens. [Genome view]
GeneID	9179.
KEGG	hsa:9179.
UCSC	uc003utb.2. human.
Organism-specific databases
CTD	9179.
GeneCards	GC07P099537.
HGNC	HGNC:574. AP4M1.
MIM	602296. gene.
PharmGKB	PA24866.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	O00189.
InParanoid	O00189.
OrthoDB	EOG96T5M2.
Gene expression databases
ArrayExpress	O00189.
Bgee	O00189.
CleanEx	HS_AP4M1.
Genevestigator	O00189.
GermOnline	ENSG00000206580. Homo sapiens.
Family and domain databases
InterPro	IPR001392. Clathrin_mu. IPR008968. Clathrin_mu_C. IPR018240. Clathrin_mu_CS. IPR000804. Clathrin_sigma/coatomer_zeta. IPR011012. Longin-like. [Graphical view]
Pfam	PF00928. Adap_comp_sub. 1 hit. PF01217. Clat_adaptor_s. 1 hit. [Graphical view]
PIRSF	PIRSF005992. Clathrin_mu. 1 hit.
PRINTS	PR00314. CLATHRINADPT.
PROSITE	PS00990. CLAT_ADAPTOR_M_1. False negative. PS00991. CLAT_ADAPTOR_M_2. 1 hit. PS51072. MHD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	34413.
SOURCE	Search...

Entry information

Entry name

AP4M1_HUMAN

Accession

Primary (citable) accession number: O00189
Secondary accession number(s): Q8WV65, Q9UHK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 17, 2007
Last modified:	December 15, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.