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Reviewed, UniProtKB/Swiss-Prot O00189 (AP4M1_HUMAN)

Last modified December 15, 2009. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    AP-4 complex subunit mu-1
Alternative name(s):
    Adapter-related protein complex 4 mu-1 subunit
    AP-4 adapter complex mu subunit
    Mu subunit of AP-4
    Mu4-adaptin
      Short name=mu4
    Mu-adaptin-related protein 2
      Short name=mu-ARP2
Gene names
Name: AP4M1
Synonyms: MUARP2
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Subunit of novel type of clathrin- or non-clathrin-associated protein coat involved in targeting proteins from the trans-Golgi network (TGN) to the endosomal-lysosomal system.

Subunit structure

Adapter-like complex 4 (AP-4) is an heterotetramer composed of two large adaptins (epsilon-type subunit AP4E1 and beta-type subunit AP4B1), a medium adaptin (mu-type subunit AP4M1) and a small adaptin (sigma-type AP4S1). Interacts with the C-terminal domain of GRID2 By similarity.

Subcellular location

Golgi apparatustrans-Golgi network. Membranecoated pit. Note: Associated with the trans-Golgi network. Found in soma and dendritic shafts of neuronal cells.

Tissue specificity

Ubiquitous. Highly expressed in testis and lowly expressed in brain and lung.

Domain

Interacts specifically with tyrosine-based sorting signals.

Sequence similarities

Belongs to the adaptor complexes medium subunit family.

Contains 1 MHD (mu homology) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 453453AP-4 complex subunit mu-1
PRO_0000193787

Regions

Domain181 – 453273MHD

Amino acid modifications

Modified residue3421Phosphoserine Ref.5

Experimental info

Sequence conflict3381R → G in AAD43328. Ref.2
Sequence conflict3451Q → R in CAA69667. Ref.1
Sequence conflict3451Q → R in AAD25869. Ref.3
Sequence conflict4001L → M in AAD43328. Ref.2
Sequence conflict4171S → C in AAD43328. Ref.2

Sequences

Sequence LengthMass (Da)Tools
O00189-1 [UniParc].

Last modified April 17, 2007. Version 2.
Checksum: AE3DCA8C5AED08B7

FASTA45349,977
        10         20         30         40         50         60 
MISQFFILSS KGDPLIYKDF RGDSGGRDVA ELFYRKLTGL PGDESPVVMH HHGRHFIHIR 

        70         80         90        100        110        120 
HSGLYLVVTT SENVSPFSLL ELLSRLATLL GDYCGSLGEG TISRNVALVY ELLDEVLDYG 

       130        140        150        160        170        180 
YVQTTSTEML RNFIQTEAVV SKPFSLFDLS SVGLFGAETQ QSKVAPSSAA SRPVLSSRSD 

       190        200        210        220        230        240 
QSQKNEVFLD VVERLSVLIA SNGSLLKVDV QGEIRLKSFL PSGSEMRIGL TEEFCVGKSE 

       250        260        270        280        290        300 
LRGYGPGIRV DEVSFHSSVN LDEFESHRIL RLQPPQGELT VMRYQLSDDL PSPLPFRLFP 

       310        320        330        340        350        360 
SVQWDRGSGR LQVYLKLRCD LLSKSQALNV RLHLPLPRGV VSLSQELSSP EQKAELAEGA 

       370        380        390        400        410        420 
LRWDLPRVQG GSQLSGLFQM DVPGPPGPPS HGLSTSASPL GLGPASLSFE LPRHTCSGLQ 

       430        440        450 
VRFLRLAFRP CGNANPHKWV RHLSHSDAYV IRI 

« Hide

References

« Hide 'large scale' references
[1]"Identification of two new mu-adaptin-related proteins, mu-ARP1 and mu-ARP2."
Wang X., Kilimann M.W.
FEBS Lett. 402:57-61(1997) [PubMed: 9013859] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Characterization of a fourth adaptor-related protein complex."
Hirst J., Bright N.A., Rous B., Robinson M.S.
Mol. Biol. Cell 10:2787-2802(1999) [PubMed: 10436028] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"Signal-binding specificity of the mu4 subunit of the adaptor protein complex, AP-4."
Aguilar R.C., Boehm M., Gorshkova I., Crouch R.J., Tomita K., Saito T., Ohno H., Bonifacino J.S.
J. Biol. Chem. 276:13145-13152(2001) [PubMed: 11139587] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Tissue: Spleen.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[5]"Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra."
Yu L.-R., Zhu Z., Chan K.C., Issaq H.J., Dimitrov D.S., Veenstra T.D.
J. Proteome Res. 6:4150-4162(2007) [PubMed: 17924679] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-342, MASS SPECTROMETRY.
Tissue: Epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

Y08387 mRNA. Translation: CAA69667.1.
AF155158 mRNA. Translation: AAD43328.1.
AF020796 mRNA. Translation: AAD25869.1.
BC018705 mRNA. Translation: AAH18705.1.
IPIIPI00103040.
RefSeqNP_004713.2.
UniGeneHs.632317

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO00189.

PTM databases

PhosphoSiteO00189.

Proteomic databases

PRIDEO00189.

Genome annotation databases

EnsemblENST00000359593; ENSP00000352603; ENSG00000221838; Homo sapiens. [Genome view]
ENST00000421755; ENSP00000412185; ENSG00000221838; Homo sapiens. [Genome view]
GeneID9179.
KEGGhsa:9179.
UCSCuc003utb.2. human.

Organism-specific databases

CTD9179.
GeneCardsGC07P099537.
HGNCHGNC:574. AP4M1.
MIM602296. gene.
PharmGKBPA24866.
GenAtlasSearch...

Phylogenomic databases

HOVERGENO00189.
InParanoidO00189.
OrthoDBEOG96T5M2.

Gene expression databases

ArrayExpressO00189.
BgeeO00189.
CleanExHS_AP4M1.
GenevestigatorO00189.
GermOnlineENSG00000206580. Homo sapiens.

Family and domain databases

InterProIPR001392. Clathrin_mu.
IPR008968. Clathrin_mu_C.
IPR018240. Clathrin_mu_CS.
IPR000804. Clathrin_sigma/coatomer_zeta.
IPR011012. Longin-like.
[Graphical view]
PfamPF00928. Adap_comp_sub. 1 hit.
PF01217. Clat_adaptor_s. 1 hit.
[Graphical view]
PIRSFPIRSF005992. Clathrin_mu. 1 hit.
PRINTSPR00314. CLATHRINADPT.
PROSITEPS00990. CLAT_ADAPTOR_M_1. False negative.
PS00991. CLAT_ADAPTOR_M_2. 1 hit.
PS51072. MHD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio34413.
SOURCESearch...

Entry information

Entry nameAP4M1_HUMAN
AccessionPrimary (citable) accession number: O00189
Secondary accession number(s): Q8WV65, Q9UHK9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 17, 2007
Last modified: December 15, 2009
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents