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O00186

- STXB3_HUMAN

UniProt

O00186 - STXB3_HUMAN

Protein

Syntaxin-binding protein 3

Gene

STXBP3

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (23 Sep 2008)
      Previous versions | rss
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    Functioni

    Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.By similarity

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. syntaxin binding Source: UniProtKB

    GO - Biological processi

    1. blood coagulation Source: Reactome
    2. membrane organization Source: Reactome
    3. negative regulation of calcium ion-dependent exocytosis Source: UniProtKB
    4. neutrophil degranulation Source: UniProtKB
    5. platelet activation Source: Reactome
    6. platelet aggregation Source: UniProtKB
    7. protein heterooligomerization Source: Ensembl
    8. protein transport Source: UniProtKB-KW
    9. vesicle docking involved in exocytosis Source: InterPro

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_1178. Disinhibition of SNARE formation.
    REACT_147867. Translocation of GLUT4 to the plasma membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Syntaxin-binding protein 3
    Alternative name(s):
    Platelet Sec1 protein
    Short name:
    PSP
    Protein unc-18 homolog 3
    Short name:
    Unc18-3
    Protein unc-18 homolog C
    Short name:
    Unc-18C
    Gene namesi
    Name:STXBP3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:11446. STXBP3.

    Subcellular locationi

    Cytoplasmcytosol 1 Publication. Cell membrane 1 Publication
    Note: In platelets, predominantly cytosolic. Low amounts membrane-associated.

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. basolateral plasma membrane Source: Ensembl
    3. cytosol Source: UniProtKB
    4. extracellular vesicular exosome Source: UniProt
    5. plasma membrane Source: UniProtKB
    6. platelet alpha granule Source: UniProtKB
    7. specific granule Source: UniProtKB
    8. tertiary granule Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA36243.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 592592Syntaxin-binding protein 3PRO_0000206285Add
    BLAST

    Post-translational modificationi

    Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO00186.
    PaxDbiO00186.
    PRIDEiO00186.

    PTM databases

    PhosphoSiteiO00186.

    Expressioni

    Tissue specificityi

    Megakaryocytes and platelets.

    Gene expression databases

    BgeeiO00186.
    CleanExiHS_STXBP3.
    GenevestigatoriO00186.

    Organism-specific databases

    HPAiHPA027225.

    Interactioni

    Subunit structurei

    Interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion By similarity. Interacts with STX4.By similarity1 Publication

    Protein-protein interaction databases

    BioGridi112683. 11 interactions.
    IntActiO00186. 3 interactions.
    MINTiMINT-4525992.
    STRINGi9606.ENSP00000359025.

    Structurei

    3D structure databases

    ProteinModelPortaliO00186.
    SMRiO00186. Positions 7-584.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 255255Mediates interaction with DOC2BBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the STXBP/unc-18/SEC1 family.Curated

    Phylogenomic databases

    eggNOGiCOG5158.
    HOGENOMiHOG000232146.
    HOVERGENiHBG052710.
    InParanoidiO00186.
    KOiK15301.
    OMAiLCCKMTD.
    OrthoDBiEOG78PV8M.
    PhylomeDBiO00186.
    TreeFamiTF313242.

    Family and domain databases

    Gene3Di3.40.50.1910. 2 hits.
    InterProiIPR027482. Sec-1-like_dom2.
    IPR001619. Sec1-like.
    [Graphical view]
    PANTHERiPTHR11679. PTHR11679. 1 hit.
    PfamiPF00995. Sec1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005715. VPS45_Sec1. 1 hit.
    SUPFAMiSSF56815. SSF56815. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O00186-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAPPVAERGL KSVVWQKIKA TVFDDCKKEG EWKIMLLDEF TTKLLASCCK    50
    MTDLLEEGIT VVENIYKNRE PVRQMKALYF ITPTSKSVDC FLHDFASKSE 100
    NKYKAAYIYF TDFCPDNLFN KIKASCSKSI RRCKEINISF IPHESQVYTL 150
    DVPDAFYYCY SPDPGNAKGK DAIMETMADQ IVTVCATLDE NPGVRYKSKP 200
    LDNASKLAQL VEKKLEDYYK IDEKSLIKGK THSQLLIIDR GFDPVSTVLH 250
    ELTFQAMAYD LLPIENDTYK YKTDGKEKEA ILEEEDDLWV RIRHRHIAVV 300
    LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ITKQVVHLNL 350
    AEDCMNKFKL NIEKLCKTEQ DLALGTDAEG QKVKDSMRVL LPVLLNKNHD 400
    NCDKIRAILL YIFSINGTTE ENLDRLIQNV KIENESDMIR NWSYLGVPIV 450
    PQSQQGKPLR KDRSAEETFQ LSRWTPFIKD IMEDAIDNRL DSKEWPYCSQ 500
    CPAVWNGSGA VSARQKPRAN YLEDRKNGSK LIVFVIGGIT YSEVRCAYEV 550
    SQAHKSCEVI IGSTHVLTPK KLLDDIKMLN KPKDKVSLIK DE 592
    Length:592
    Mass (Da):67,764
    Last modified:September 23, 2008 - v2
    Checksum:iAAFA268E2D328EC7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti104 – 1041K → R in AAH38099. (PubMed:15489334)Curated
    Sequence conflicti150 – 1501L → R in AAH38099. (PubMed:15489334)Curated
    Sequence conflicti242 – 2421F → I in BAD96890. 1 PublicationCurated
    Sequence conflicti277 – 2771E → G in BAA19482. (PubMed:8824310)Curated
    Sequence conflicti285 – 2851E → D in BAF84011. (PubMed:14702039)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti295 – 2951R → Q.
    Corresponds to variant rs2275344 [ dbSNP | Ensembl ].
    VAR_052470
    Natural varianti433 – 4331E → G.1 Publication
    Corresponds to variant rs1044136 [ dbSNP | Ensembl ].
    VAR_017570
    Natural varianti546 – 5461C → G.1 Publication
    Corresponds to variant rs1044137 [ dbSNP | Ensembl ].
    VAR_017571

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63506 mRNA. Translation: BAA19482.1.
    AF032922 mRNA. Translation: AAC69606.1.
    AK290134 mRNA. Translation: BAF82823.1.
    AK291322 mRNA. Translation: BAF84011.1.
    AK312910 mRNA. Translation: BAG35756.1.
    AK223170 mRNA. Translation: BAD96890.1.
    AL591719, AL449266 Genomic DNA. Translation: CAH72066.1.
    AL449266, AL591719 Genomic DNA. Translation: CAI14350.1.
    CH471122 Genomic DNA. Translation: EAW56330.1.
    BC038099 mRNA. Translation: AAH38099.1.
    BC047764 mRNA. Translation: AAH47764.1.
    CCDSiCCDS790.1.
    RefSeqiNP_009200.2. NM_007269.2.
    UniGeneiHs.530436.

    Genome annotation databases

    EnsembliENST00000370008; ENSP00000359025; ENSG00000116266.
    GeneIDi6814.
    KEGGihsa:6814.
    UCSCiuc001dvy.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D63506 mRNA. Translation: BAA19482.1 .
    AF032922 mRNA. Translation: AAC69606.1 .
    AK290134 mRNA. Translation: BAF82823.1 .
    AK291322 mRNA. Translation: BAF84011.1 .
    AK312910 mRNA. Translation: BAG35756.1 .
    AK223170 mRNA. Translation: BAD96890.1 .
    AL591719 , AL449266 Genomic DNA. Translation: CAH72066.1 .
    AL449266 , AL591719 Genomic DNA. Translation: CAI14350.1 .
    CH471122 Genomic DNA. Translation: EAW56330.1 .
    BC038099 mRNA. Translation: AAH38099.1 .
    BC047764 mRNA. Translation: AAH47764.1 .
    CCDSi CCDS790.1.
    RefSeqi NP_009200.2. NM_007269.2.
    UniGenei Hs.530436.

    3D structure databases

    ProteinModelPortali O00186.
    SMRi O00186. Positions 7-584.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 112683. 11 interactions.
    IntActi O00186. 3 interactions.
    MINTi MINT-4525992.
    STRINGi 9606.ENSP00000359025.

    PTM databases

    PhosphoSitei O00186.

    Proteomic databases

    MaxQBi O00186.
    PaxDbi O00186.
    PRIDEi O00186.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000370008 ; ENSP00000359025 ; ENSG00000116266 .
    GeneIDi 6814.
    KEGGi hsa:6814.
    UCSCi uc001dvy.3. human.

    Organism-specific databases

    CTDi 6814.
    GeneCardsi GC01P109289.
    HGNCi HGNC:11446. STXBP3.
    HPAi HPA027225.
    MIMi 608339. gene.
    neXtProti NX_O00186.
    PharmGKBi PA36243.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5158.
    HOGENOMi HOG000232146.
    HOVERGENi HBG052710.
    InParanoidi O00186.
    KOi K15301.
    OMAi LCCKMTD.
    OrthoDBi EOG78PV8M.
    PhylomeDBi O00186.
    TreeFami TF313242.

    Enzyme and pathway databases

    Reactomei REACT_1178. Disinhibition of SNARE formation.
    REACT_147867. Translocation of GLUT4 to the plasma membrane.

    Miscellaneous databases

    ChiTaRSi STXBP3. human.
    GeneWikii Syntaxin_binding_protein_3.
    GenomeRNAii 6814.
    NextBioi 26593.
    PROi O00186.
    SOURCEi Search...

    Gene expression databases

    Bgeei O00186.
    CleanExi HS_STXBP3.
    Genevestigatori O00186.

    Family and domain databases

    Gene3Di 3.40.50.1910. 2 hits.
    InterProi IPR027482. Sec-1-like_dom2.
    IPR001619. Sec1-like.
    [Graphical view ]
    PANTHERi PTHR11679. PTHR11679. 1 hit.
    Pfami PF00995. Sec1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005715. VPS45_Sec1. 1 hit.
    SUPFAMi SSF56815. SSF56815. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants."
      Gengyo-Ando K., Kitayama H., Mukaida M., Ikawa Y.
      J. Neurosci. 16:6695-6702(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-433 AND GLY-546.
      Tissue: Brain.
    2. "Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion."
      Reed G.L., Houng A.K., Fitzgerald M.L.
      Blood 93:2617-2626(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH STX4, SUBCELLULAR LOCATION, PHOSPHORYLATION.
      Tissue: Leukemic T-cell.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thalamus and Tongue.
    4. Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
      Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.
    5. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSTXB3_HUMAN
    AccessioniPrimary (citable) accession number: O00186
    Secondary accession number(s): A8K269
    , A8K5K7, Q53FW1, Q86YJ3, Q9UPD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: September 23, 2008
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3