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O00186 (STXB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Syntaxin-binding protein 3
Alternative name(s):
Platelet Sec1 protein
Short name=PSP
Protein unc-18 homolog 3
Short name=Unc18-3
Protein unc-18 homolog C
Short name=Unc-18C
Gene names
Name:STXBP3
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length592 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes By similarity.

Subunit structure

Interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion By similarity. Interacts with STX4. Ref.2

Subcellular location

Cytoplasmcytosol. Cell membrane. Note: In platelets, predominantly cytosolic. Low amounts membrane-associated. Ref.2

Tissue specificity

Megakaryocytes and platelets.

Post-translational modification

Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4. Ref.2

Sequence similarities

Belongs to the STXBP/unc-18/SEC1 family.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCell membrane
Cytoplasm
Membrane
   Coding sequence diversityPolymorphism
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processblood coagulation

Traceable author statement. Source: Reactome

membrane organization

Traceable author statement. Source: Reactome

negative regulation of calcium ion-dependent exocytosis

Inferred from mutant phenotype PubMed 12649283. Source: UniProtKB

neutrophil degranulation

Inferred from expression pattern PubMed 18588921. Source: UniProtKB

platelet activation

Traceable author statement. Source: Reactome

platelet aggregation

Inferred from mutant phenotype PubMed 12773094. Source: UniProtKB

protein heterooligomerization

Inferred from electronic annotation. Source: Ensembl

protein transport

Inferred from electronic annotation. Source: UniProtKB-KW

vesicle docking involved in exocytosis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: Ensembl

basolateral plasma membrane

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from direct assay PubMed 18588921. Source: UniProtKB

extracellular vesicular exosome

Inferred from direct assay PubMed 20458337. Source: UniProt

plasma membrane

Inferred from direct assay PubMed 18588921. Source: UniProtKB

platelet alpha granule

Inferred from direct assay PubMed 12773094. Source: UniProtKB

specific granule

Inferred from direct assay PubMed 18588921. Source: UniProtKB

tertiary granule

Inferred from direct assay PubMed 18588921. Source: UniProtKB

   Molecular_functionsyntaxin binding

Inferred from physical interaction PubMed 12773094PubMed 15576373. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 592592Syntaxin-binding protein 3
PRO_0000206285

Regions

Region1 – 255255Mediates interaction with DOC2B By similarity

Natural variations

Natural variant2951R → Q.
Corresponds to variant rs2275344 [ dbSNP | Ensembl ].
VAR_052470
Natural variant4331E → G. Ref.1
Corresponds to variant rs1044136 [ dbSNP | Ensembl ].
VAR_017570
Natural variant5461C → G. Ref.1
Corresponds to variant rs1044137 [ dbSNP | Ensembl ].
VAR_017571

Experimental info

Sequence conflict1041K → R in AAH38099. Ref.7
Sequence conflict1501L → R in AAH38099. Ref.7
Sequence conflict2421F → I in BAD96890. Ref.4
Sequence conflict2771E → G in BAA19482. Ref.1
Sequence conflict2851E → D in BAF84011. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O00186 [UniParc].

Last modified September 23, 2008. Version 2.
Checksum: AAFA268E2D328EC7

FASTA59267,764
        10         20         30         40         50         60 
MAPPVAERGL KSVVWQKIKA TVFDDCKKEG EWKIMLLDEF TTKLLASCCK MTDLLEEGIT 

        70         80         90        100        110        120 
VVENIYKNRE PVRQMKALYF ITPTSKSVDC FLHDFASKSE NKYKAAYIYF TDFCPDNLFN 

       130        140        150        160        170        180 
KIKASCSKSI RRCKEINISF IPHESQVYTL DVPDAFYYCY SPDPGNAKGK DAIMETMADQ 

       190        200        210        220        230        240 
IVTVCATLDE NPGVRYKSKP LDNASKLAQL VEKKLEDYYK IDEKSLIKGK THSQLLIIDR 

       250        260        270        280        290        300 
GFDPVSTVLH ELTFQAMAYD LLPIENDTYK YKTDGKEKEA ILEEEDDLWV RIRHRHIAVV 

       310        320        330        340        350        360 
LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ITKQVVHLNL AEDCMNKFKL 

       370        380        390        400        410        420 
NIEKLCKTEQ DLALGTDAEG QKVKDSMRVL LPVLLNKNHD NCDKIRAILL YIFSINGTTE 

       430        440        450        460        470        480 
ENLDRLIQNV KIENESDMIR NWSYLGVPIV PQSQQGKPLR KDRSAEETFQ LSRWTPFIKD 

       490        500        510        520        530        540 
IMEDAIDNRL DSKEWPYCSQ CPAVWNGSGA VSARQKPRAN YLEDRKNGSK LIVFVIGGIT 

       550        560        570        580        590 
YSEVRCAYEV SQAHKSCEVI IGSTHVLTPK KLLDDIKMLN KPKDKVSLIK DE 

« Hide

References

« Hide 'large scale' references
[1]"A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants."
Gengyo-Ando K., Kitayama H., Mukaida M., Ikawa Y.
J. Neurosci. 16:6695-6702(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-433 AND GLY-546.
Tissue: Brain.
[2]"Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion."
Reed G.L., Houng A.K., Fitzgerald M.L.
Blood 93:2617-2626(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH STX4, SUBCELLULAR LOCATION, PHOSPHORYLATION.
Tissue: Leukemic T-cell.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thalamus and Tongue.
[4]Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S.
Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D63506 mRNA. Translation: BAA19482.1.
AF032922 mRNA. Translation: AAC69606.1.
AK290134 mRNA. Translation: BAF82823.1.
AK291322 mRNA. Translation: BAF84011.1.
AK312910 mRNA. Translation: BAG35756.1.
AK223170 mRNA. Translation: BAD96890.1.
AL591719, AL449266 Genomic DNA. Translation: CAH72066.1.
AL449266, AL591719 Genomic DNA. Translation: CAI14350.1.
CH471122 Genomic DNA. Translation: EAW56330.1.
BC038099 mRNA. Translation: AAH38099.1.
BC047764 mRNA. Translation: AAH47764.1.
RefSeqNP_009200.2. NM_007269.2.
UniGeneHs.530436.

3D structure databases

ProteinModelPortalO00186.
SMRO00186. Positions 7-584.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid112683. 11 interactions.
IntActO00186. 3 interactions.
MINTMINT-4525992.
STRING9606.ENSP00000359025.

PTM databases

PhosphoSiteO00186.

Proteomic databases

PaxDbO00186.
PRIDEO00186.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000370008; ENSP00000359025; ENSG00000116266.
GeneID6814.
KEGGhsa:6814.
UCSCuc001dvy.3. human.

Organism-specific databases

CTD6814.
GeneCardsGC01P109289.
HGNCHGNC:11446. STXBP3.
HPAHPA027225.
MIM608339. gene.
neXtProtNX_O00186.
PharmGKBPA36243.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5158.
HOGENOMHOG000232146.
HOVERGENHBG052710.
InParanoidO00186.
KOK15301.
OMACKKEDEW.
OrthoDBEOG78PV8M.
PhylomeDBO00186.
TreeFamTF313242.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.
REACT_604. Hemostasis.

Gene expression databases

BgeeO00186.
CleanExHS_STXBP3.
GenevestigatorO00186.

Family and domain databases

Gene3D3.40.50.1910. 2 hits.
InterProIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
[Graphical view]
PANTHERPTHR11679. PTHR11679. 1 hit.
PfamPF00995. Sec1. 1 hit.
[Graphical view]
PIRSFPIRSF005715. VPS45_Sec1. 1 hit.
SUPFAMSSF56815. SSF56815. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSTXBP3. human.
GeneWikiSyntaxin_binding_protein_3.
GenomeRNAi6814.
NextBio26593.
PROO00186.
SOURCESearch...

Entry information

Entry nameSTXB3_HUMAN
AccessionPrimary (citable) accession number: O00186
Secondary accession number(s): A8K269 expand/collapse secondary AC list , A8K5K7, Q53FW1, Q86YJ3, Q9UPD7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 23, 2008
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM