O00186 (STXB3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-binding protein 3

Alternative name(s):
Platelet Sec1 protein
Short name=PSP
Protein unc-18 homolog 3
Short name=Unc18-3
Protein unc-18 homolog C
Short name=Unc-18C

Gene names

Name:

STXBP3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes By similarity.
Subunit structure	Interacts with DOC2B; the interaction is direct, occurs at the cell membrane, excludes interaction with STX4 and regulates glucose-stimulated insulin secretion By similarity. Interacts with STX4. Ref.2
Subcellular location	Cytoplasm › cytosol. Cell membrane. Note: In platelets, predominantly cytosolic. Low amounts membrane-associated. Ref.2
Tissue specificity	Megakaryocytes and platelets.
Post-translational modification	Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4. Ref.2
Sequence similarities	Belongs to the STXBP/unc-18/SEC1 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cell membrane Cytoplasm Membrane
Coding sequence diversity	Polymorphism
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of calcium ion-dependent exocytosis Inferred from mutant phenotype PubMed 12649283. Source: UniProtKB neutrophil degranulation Inferred from expression pattern PubMed 18588921. Source: UniProtKB platelet aggregation Inferred from mutant phenotype PubMed 12773094. Source: UniProtKB protein heterooligomerization Inferred from electronic annotation. Source: Compara protein transport Inferred from electronic annotation. Source: UniProtKB-KW vesicle docking involved in exocytosis Inferred from electronic annotation. Source: InterPro
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara basolateral plasma membrane Inferred from electronic annotation. Source: Compara cytosol Inferred from direct assay PubMed 18588921. Source: UniProtKB nucleus Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay PubMed 18588921. Source: UniProtKB platelet alpha granule Inferred from direct assay PubMed 12773094. Source: UniProtKB specific granule Inferred from direct assay PubMed 18588921. Source: UniProtKB tertiary granule Inferred from direct assay PubMed 18588921. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 592	592	Syntaxin-binding protein 3		PRO_0000206285
Regions
	Region	1 – 255	255	Mediates interaction with DOC2B By similarity
Amino acid modifications
	Modified residue	198	1	Phosphoserine By similarity
Natural variations
	Natural variant	295	1	R → Q. Corresponds to variant rs2275344 [ dbSNP | Ensembl ].		VAR_052470
	Natural variant	433	1	E → G. Ref.1 Corresponds to variant rs1044136 [ dbSNP | Ensembl ].		VAR_017570
	Natural variant	546	1	C → G. Ref.1 Corresponds to variant rs1044137 [ dbSNP | Ensembl ].		VAR_017571
Experimental info
	Sequence conflict	104	1	K → R in AAH38099. Ref.7
	Sequence conflict	150	1	L → R in AAH38099. Ref.7
	Sequence conflict	242	1	F → I in BAD96890. Ref.4
	Sequence conflict	277	1	E → G in BAA19482. Ref.1
	Sequence conflict	285	1	E → D in BAF84011. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O00186 [UniParc]. Last modified September 23, 2008. Version 2. Checksum: AAFA268E2D328EC7	FASTA	592	67,764
	10 20 30 40 50 60 MAPPVAERGL KSVVWQKIKA TVFDDCKKEG EWKIMLLDEF TTKLLASCCK MTDLLEEGIT 70 80 90 100 110 120 VVENIYKNRE PVRQMKALYF ITPTSKSVDC FLHDFASKSE NKYKAAYIYF TDFCPDNLFN 130 140 150 160 170 180 KIKASCSKSI RRCKEINISF IPHESQVYTL DVPDAFYYCY SPDPGNAKGK DAIMETMADQ 190 200 210 220 230 240 IVTVCATLDE NPGVRYKSKP LDNASKLAQL VEKKLEDYYK IDEKSLIKGK THSQLLIIDR 250 260 270 280 290 300 GFDPVSTVLH ELTFQAMAYD LLPIENDTYK YKTDGKEKEA ILEEEDDLWV RIRHRHIAVV 310 320 330 340 350 360 LEEIPKLMKE ISSTKKATEG KTSLSALTQL MKKMPHFRKQ ITKQVVHLNL AEDCMNKFKL 370 380 390 400 410 420 NIEKLCKTEQ DLALGTDAEG QKVKDSMRVL LPVLLNKNHD NCDKIRAILL YIFSINGTTE 430 440 450 460 470 480 ENLDRLIQNV KIENESDMIR NWSYLGVPIV PQSQQGKPLR KDRSAEETFQ LSRWTPFIKD 490 500 510 520 530 540 IMEDAIDNRL DSKEWPYCSQ CPAVWNGSGA VSARQKPRAN YLEDRKNGSK LIVFVIGGIT 550 560 570 580 590 YSEVRCAYEV SQAHKSCEVI IGSTHVLTPK KLLDDIKMLN KPKDKVSLIK DE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A murine neural-specific homolog corrects cholinergic defects in Caenorhabditis elegans unc-18 mutants." Gengyo-Ando K., Kitayama H., Mukaida M., Ikawa Y. J. Neurosci. 16:6695-6702(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS GLY-433 AND GLY-546. Tissue: Brain.
[2]	"Human platelets contain SNARE proteins and a Sec1p homologue that interacts with syntaxin 4 and is phosphorylated after thrombin activation: implications for platelet secretion." Reed G.L., Houng A.K., Fitzgerald M.L. Blood 93:2617-2626(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH STX4, SUBCELLULAR LOCATION, PHOSPHORYLATION. Tissue: Leukemic T-cell.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Thalamus and Tongue.
[4]	Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S. Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D63506 mRNA. Translation: BAA19482.1. AF032922 mRNA. Translation: AAC69606.1. AK290134 mRNA. Translation: BAF82823.1. AK291322 mRNA. Translation: BAF84011.1. AK312910 mRNA. Translation: BAG35756.1. AK223170 mRNA. Translation: BAD96890.1. AL591719, AL449266 Genomic DNA. Translation: CAH72066.1. AL449266, AL591719 Genomic DNA. Translation: CAI14350.1. CH471122 Genomic DNA. Translation: EAW56330.1. BC038099 mRNA. Translation: AAH38099.1. BC047764 mRNA. Translation: AAH47764.1.
IPI	IPI00297626.
RefSeq	NP_009200.2. NM_007269.2.
UniGene	Hs.530436.
3D structure databases
ProteinModelPortal	O00186.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00186. 3 interactions.
STRING	9606.ENSP00000359025.
PTM databases
PhosphoSite	O00186.
Proteomic databases
PaxDb	O00186.
PRIDE	O00186.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000370008; ENSP00000359025; ENSG00000116266.
GeneID	6814.
KEGG	hsa:6814.
UCSC	uc001dvy.3. human.
Organism-specific databases
CTD	6814.
GeneCards	GC01P109289.
HGNC	HGNC:11446. STXBP3.
HPA	HPA027225.
MIM	608339. gene.
neXtProt	NX_O00186.
PharmGKB	PA36243.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5158.
HOGENOM	HOG000232146.
HOVERGEN	HBG052710.
InParanoid	O00186.
KO	K15301.
OMA	CKKEDEW.
OrthoDB	EOG44J2HV.
PhylomeDB	O00186.
Enzyme and pathway databases
Reactome	REACT_11123. Membrane Trafficking. REACT_604. Hemostasis.
Gene expression databases
Bgee	O00186.
CleanEx	HS_STXBP3.
Genevestigator	O00186.
GermOnline	ENSG00000116266. Homo sapiens.
Family and domain databases
InterPro	IPR001619. Sec1-like. [Graphical view]
PANTHER	PTHR11679. PTHR11679. 1 hit.
Pfam	PF00995. Sec1. 1 hit. [Graphical view]
PIRSF	PIRSF005715. VPS45_Sec1. 1 hit.
SUPFAM	SSF56815. Sec1-like. 1 hit.
ProtoNet	Search...
Other
ChiTaRS	STXBP3. human.
GenomeRNAi	6814.
NextBio	26593.
SOURCE	Search...

Entry information

Entry name

STXB3_HUMAN

Accession

Primary (citable) accession number: O00186
Secondary accession number(s): A8K269 , A8K5K7, Q53FW1, Q86YJ3, Q9UPD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	September 23, 2008
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.