Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Potassium channel subfamily K member 1

Gene

KCNK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Weakly inward rectifying potassium channel that contributes to the regulation of the resting membrane potential.2 Publications

Enzyme regulationi

Inhibited by Ba2+ ions, quinine and quinidine (PubMed:8605869). Is not inhibited by 4-aminopyridine, charybdotoxin and dendrotoxin, and is only marginally inhibited by 10 mM TEA (PubMed:8605869).1 Publication

Kineticsi

Activation is very rapid, but inactivation is very slow.1 Publication

      GO - Molecular functioni

      • inward rectifier potassium channel activity Source: ProtInc
      • potassium channel activity Source: UniProtKB
      • potassium ion leak channel activity Source: GO_Central

      GO - Biological processi

      • potassium ion transmembrane transport Source: UniProtKB
      • potassium ion transport Source: ProtInc
      • response to nicotine Source: Ensembl
      • stabilization of membrane potential Source: GO_Central
      • synaptic transmission Source: Reactome
      Complete GO annotation...

      Keywords - Molecular functioni

      Ion channel, Potassium channel, Voltage-gated channel

      Keywords - Biological processi

      Ion transport, Potassium transport, Transport

      Keywords - Ligandi

      Potassium

      Enzyme and pathway databases

      ReactomeiREACT_75779. Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK).

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Potassium channel subfamily K member 1
      Alternative name(s):
      Inward rectifying potassium channel protein TWIK-1
      Potassium channel KCNO1
      Gene namesi
      Name:KCNK1
      Synonyms:HOHO1, KCNO1, TWIK1
      OrganismiHomo sapiens (Human)
      Taxonomic identifieri9606 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
      ProteomesiUP000005640 Componenti: Chromosome 1

      Organism-specific databases

      HGNCiHGNC:6272. KCNK1.

      Subcellular locationi

      • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

      Topology

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Topological domaini1 – 2020Cytoplasmic1 PublicationAdd
      BLAST
      Transmembranei21 – 4121Helical1 PublicationAdd
      BLAST
      Topological domaini42 – 10362Extracellular1 PublicationAdd
      BLAST
      Intramembranei104 – 11613Helical; Name=Pore helix 11 PublicationAdd
      BLAST
      Intramembranei117 – 12261 Publication
      Topological domaini123 – 13210Extracellular1 Publication
      Transmembranei133 – 15624Helical1 PublicationAdd
      BLAST
      Topological domaini157 – 18125Cytoplasmic1 PublicationAdd
      BLAST
      Transmembranei182 – 20221Helical1 PublicationAdd
      BLAST
      Topological domaini203 – 2119Extracellular1 Publication
      Intramembranei212 – 22413Helical; Name=Pore helix 21 PublicationAdd
      BLAST
      Intramembranei225 – 23171 Publication
      Topological domaini232 – 24312Extracellular1 PublicationAdd
      BLAST
      Transmembranei244 – 26724Helical1 PublicationAdd
      BLAST
      Topological domaini268 – 33669Cytoplasmic1 PublicationAdd
      BLAST

      GO - Cellular componenti

      • apical plasma membrane Source: Ensembl
      • brush border membrane Source: Ensembl
      • endosome Source: Ensembl
      • integral component of membrane Source: UniProtKB
      • integral component of plasma membrane Source: UniProtKB
      • plasma membrane Source: Reactome
      • potassium channel complex Source: UniProtKB
      • voltage-gated potassium channel complex Source: ProtInc
      Complete GO annotation...

      Keywords - Cellular componenti

      Cell membrane, Membrane

      Pathology & Biotechi

      Mutagenesis

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Mutagenesisi161 – 1611T → A: No effect on channel activity. 1 Publication

      Organism-specific databases

      PharmGKBiPA219.

      Chemistry

      DrugBankiDB00308. Ibutilide.
      DB00908. Quinidine.
      DB01346. Quinidine barbiturate.

      Polymorphism and mutation databases

      BioMutaiKCNK1.

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Chaini1 – 336336Potassium channel subfamily K member 1PRO_0000101740Add
      BLAST

      Amino acid modifications

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Disulfide bondi69 – 69Interchain1 Publication
      Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis

      Keywords - PTMi

      Disulfide bond, Glycoprotein

      Proteomic databases

      MaxQBiO00180.
      PaxDbiO00180.
      PRIDEiO00180.

      PTM databases

      PhosphoSiteiO00180.

      Expressioni

      Tissue specificityi

      Widely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney (PubMed:8605869).1 Publication

      Gene expression databases

      BgeeiO00180.
      CleanExiHS_KCNK1.
      ExpressionAtlasiO00180. baseline and differential.
      GenevisibleiO00180. HS.

      Organism-specific databases

      HPAiCAB022588.
      HPA016049.

      Interactioni

      Subunit structurei

      Homodimer; disulfide-linked.1 Publication

      Protein-protein interaction databases

      BioGridi109976. 5 interactions.
      DIPiDIP-59532N.
      IntActiO00180. 1 interaction.
      STRINGi9606.ENSP00000355580.

      Structurei

      Secondary structure

      1
      336
      Legend: HelixTurnBeta strand
      Show more details
      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Helixi19 – 6648Combined sources
      Helixi72 – 8615Combined sources
      Turni87 – 893Combined sources
      Helixi104 – 11512Combined sources
      Helixi128 – 16033Combined sources
      Turni163 – 1675Combined sources
      Helixi177 – 19519Combined sources
      Helixi197 – 20610Combined sources
      Beta strandi207 – 2093Combined sources
      Helixi212 – 22312Combined sources
      Beta strandi236 – 2383Combined sources
      Helixi242 – 26827Combined sources
      Helixi271 – 2788Combined sources

      3D structure databases

      Select the link destinations:
      PDBei
      RCSB PDBi
      PDBji
      Links Updated
      EntryMethodResolution (Å)ChainPositionsPDBsum
      3UKMX-ray3.40A/B/C/D19-288[»]
      ProteinModelPortaliO00180.
      SMRiO00180. Positions 19-281.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Region

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Regioni117 – 1226Selectivity filter 11 Publication
      Regioni225 – 2306Selectivity filter 21 Publication

      Sequence similaritiesi

      Keywords - Domaini

      Transmembrane, Transmembrane helix

      Phylogenomic databases

      eggNOGiCOG1226.
      GeneTreeiENSGT00760000118858.
      HOGENOMiHOG000286014.
      HOVERGENiHBG052237.
      InParanoidiO00180.
      KOiK04912.
      OMAiKQLRKMF.
      OrthoDBiEOG7TTQ85.
      PhylomeDBiO00180.
      TreeFamiTF313947.

      Family and domain databases

      InterProiIPR003280. 2pore_dom_K_chnl.
      IPR013099. 2pore_dom_K_chnl_dom.
      IPR003092. 2pore_dom_K_chnl_TASK.
      IPR005408. 2pore_dom_K_chnl_TWIK.
      IPR001779. 2pore_dom_K_chnl_TWIK1.
      [Graphical view]
      PfamiPF07885. Ion_trans_2. 2 hits.
      [Graphical view]
      PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
      PRINTSiPR01333. 2POREKCHANEL.
      PR01096. TWIK1CHANNEL.
      PR01586. TWIKCHANNEL.

      Sequencei

      Sequence statusi: Complete.

      O00180-1 [UniParc]FASTAAdd to basket

      « Hide

              10         20         30         40         50
      MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED
      60 70 80 90 100
      LLRQELRKLK RRFLEEHECL SEQQLEQFLG RVLEASNYGV SVLSNASGNW
      110 120 130 140 150
      NWDFTSALFF ASTVLSTTGY GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT
      160 170 180 190 200
      AVVQRITVHV TRRPVLYFHI RWGFSKQVVA IVHAVLLGFV TVSCFFFIPA
      210 220 230 240 250
      AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK FRELYKIGIT
      260 270 280 290 300
      CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDQ VHIIEHDQLS
      310 320 330
      FSSITDQAAG MKEDQKQNEP FVATQSSACV DGPANH
      Length:336
      Mass (Da):38,143
      Last modified:July 1, 1997 - v1
      Checksum:i2A41D9501323215D
      GO

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      U33632 mRNA. Translation: AAB01688.1.
      U76996 mRNA. Translation: AAB97878.1.
      U90065 mRNA. Translation: AAB51147.1.
      AL356357 Genomic DNA. Translation: CAI21792.1.
      CH471098 Genomic DNA. Translation: EAW69989.1.
      BC018051 mRNA. Translation: AAH18051.1.
      CCDSiCCDS1599.1.
      PIRiS65566.
      RefSeqiNP_002236.1. NM_002245.3.
      UniGeneiHs.208544.

      Genome annotation databases

      EnsembliENST00000366621; ENSP00000355580; ENSG00000135750.
      GeneIDi3775.
      KEGGihsa:3775.
      UCSCiuc010pxo.1. human.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBLi
      GenBanki
      DDBJi
      Links Updated
      U33632 mRNA. Translation: AAB01688.1.
      U76996 mRNA. Translation: AAB97878.1.
      U90065 mRNA. Translation: AAB51147.1.
      AL356357 Genomic DNA. Translation: CAI21792.1.
      CH471098 Genomic DNA. Translation: EAW69989.1.
      BC018051 mRNA. Translation: AAH18051.1.
      CCDSiCCDS1599.1.
      PIRiS65566.
      RefSeqiNP_002236.1. NM_002245.3.
      UniGeneiHs.208544.

      3D structure databases

      Select the link destinations:
      PDBei
      RCSB PDBi
      PDBji
      Links Updated
      EntryMethodResolution (Å)ChainPositionsPDBsum
      3UKMX-ray3.40A/B/C/D19-288[»]
      ProteinModelPortaliO00180.
      SMRiO00180. Positions 19-281.
      ModBaseiSearch...
      MobiDBiSearch...

      Protein-protein interaction databases

      BioGridi109976. 5 interactions.
      DIPiDIP-59532N.
      IntActiO00180. 1 interaction.
      STRINGi9606.ENSP00000355580.

      Chemistry

      DrugBankiDB00308. Ibutilide.
      DB00908. Quinidine.
      DB01346. Quinidine barbiturate.

      PTM databases

      PhosphoSiteiO00180.

      Polymorphism and mutation databases

      BioMutaiKCNK1.

      Proteomic databases

      MaxQBiO00180.
      PaxDbiO00180.
      PRIDEiO00180.

      Protocols and materials databases

      DNASUi3775.
      Structural Biology KnowledgebaseSearch...

      Genome annotation databases

      EnsembliENST00000366621; ENSP00000355580; ENSG00000135750.
      GeneIDi3775.
      KEGGihsa:3775.
      UCSCiuc010pxo.1. human.

      Organism-specific databases

      CTDi3775.
      GeneCardsiGC01P233750.
      HGNCiHGNC:6272. KCNK1.
      HPAiCAB022588.
      HPA016049.
      MIMi601745. gene.
      neXtProtiNX_O00180.
      PharmGKBiPA219.
      GenAtlasiSearch...

      Phylogenomic databases

      eggNOGiCOG1226.
      GeneTreeiENSGT00760000118858.
      HOGENOMiHOG000286014.
      HOVERGENiHBG052237.
      InParanoidiO00180.
      KOiK04912.
      OMAiKQLRKMF.
      OrthoDBiEOG7TTQ85.
      PhylomeDBiO00180.
      TreeFamiTF313947.

      Enzyme and pathway databases

      ReactomeiREACT_75779. Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK).

      Miscellaneous databases

      ChiTaRSiKCNK1. human.
      GeneWikiiKCNK1.
      GenomeRNAii3775.
      NextBioi14807.
      PROiO00180.
      SOURCEiSearch...

      Gene expression databases

      BgeeiO00180.
      CleanExiHS_KCNK1.
      ExpressionAtlasiO00180. baseline and differential.
      GenevisibleiO00180. HS.

      Family and domain databases

      InterProiIPR003280. 2pore_dom_K_chnl.
      IPR013099. 2pore_dom_K_chnl_dom.
      IPR003092. 2pore_dom_K_chnl_TASK.
      IPR005408. 2pore_dom_K_chnl_TWIK.
      IPR001779. 2pore_dom_K_chnl_TWIK1.
      [Graphical view]
      PfamiPF07885. Ion_trans_2. 2 hits.
      [Graphical view]
      PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
      PRINTSiPR01333. 2POREKCHANEL.
      PR01096. TWIK1CHANNEL.
      PR01586. TWIKCHANNEL.
      ProtoNetiSearch...

      Publicationsi

      « Hide 'large scale' publications
      1. "TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure."
        Lesage F., Guillemare E., Fink M., Duprat F., Lazdunski M., Romey G., Barhanin J.
        EMBO J. 15:1004-1011(1996) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-161, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
        Tissue: Kidney.
      2. "Sequence and function of the two P domain potassium channels: implications of an emerging superfamily."
        Goldstein S.A.N., Wang K.-W., Ilan N., Pausch M.H.
        J. Mol. Med. 76:13-20(1998) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA], REVIEW.
        Tissue: Brain.
      3. "Cloning and localization of a double-pore K channel, KCNK1: exclusive expression in distal nephron segments."
        Orias M., Velazquez H., Tung F., Lee G., Desir G.V.
        Am. J. Physiol. 273:F663-F666(1997) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      4. "The DNA sequence and biological annotation of human chromosome 1."
        Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
        , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
        Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
        The MGC Project Team
        Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
        Tissue: Brain.
      7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      8. "Crystal structure of the human two-pore domain potassium channel K2P1."
        Miller A.N., Long S.B.
        Science 335:432-436(2012) [PubMed] [Europe PMC] [Abstract]
        Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 23-288 IN COMPLEX WITH POTASSIUM IONS, FUNCTION, SUBUNIT, DISULFIDE BOND, TOPOLOGY, SUBCELLULAR LOCATION.

      Entry informationi

      Entry nameiKCNK1_HUMAN
      AccessioniPrimary (citable) accession number: O00180
      Secondary accession number(s): Q13307, Q5T5E8
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: February 21, 2001
      Last sequence update: July 1, 1997
      Last modified: June 24, 2015
      This is version 140 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programChordata Protein Annotation Program
      DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

      Miscellaneousi

      Keywords - Technical termi

      3D-structure, Complete proteome, Reference proteome

      Documents

      1. Human chromosome 1
        Human chromosome 1: entries, gene names and cross-references to MIM
      2. MIM cross-references
        Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
      3. PDB cross-references
        Index of Protein Data Bank (PDB) cross-references
      4. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3

      Similar proteinsi

      Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
      100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
      90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
      50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.