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Protein

Potassium channel subfamily K member 1

Gene

KCNK1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Weakly inward rectifying potassium channel that contributes to the regulation of the resting membrane potential.2 Publications

Enzyme regulationi

Inhibited by Ba2+ ions, quinine and quinidine (PubMed:8605869). Is not inhibited by 4-aminopyridine, charybdotoxin and dendrotoxin, and is only marginally inhibited by 10 mM TEA (PubMed:8605869).1 Publication

Kineticsi

Activation is very rapid, but inactivation is very slow.1 Publication

    GO - Molecular functioni

    1. inward rectifier potassium channel activity Source: ProtInc

    GO - Biological processi

    1. potassium ion transport Source: ProtInc
    2. response to nicotine Source: Ensembl
    3. synaptic transmission Source: Reactome
    Complete GO annotation...

    Keywords - Molecular functioni

    Ion channel, Potassium channel, Voltage-gated channel

    Keywords - Biological processi

    Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    Potassium

    Enzyme and pathway databases

    ReactomeiREACT_75779. Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium channel subfamily K member 1
    Alternative name(s):
    Inward rectifying potassium channel protein TWIK-1
    Potassium channel KCNO1
    Gene namesi
    Name:KCNK1
    Synonyms:HOHO1, KCNO1, TWIK1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640 Componenti: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:6272. KCNK1.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2020Cytoplasmic1 PublicationAdd
    BLAST
    Transmembranei21 – 4121Helical1 PublicationAdd
    BLAST
    Topological domaini42 – 10362Extracellular1 PublicationAdd
    BLAST
    Intramembranei104 – 11613Helical; Name=Pore helix 11 PublicationAdd
    BLAST
    Intramembranei117 – 12261 Publication
    Topological domaini123 – 13210Extracellular1 Publication
    Transmembranei133 – 15624Helical1 PublicationAdd
    BLAST
    Topological domaini157 – 18125Cytoplasmic1 PublicationAdd
    BLAST
    Transmembranei182 – 20221Helical1 PublicationAdd
    BLAST
    Topological domaini203 – 2119Extracellular1 Publication
    Intramembranei212 – 22413Helical; Name=Pore helix 21 PublicationAdd
    BLAST
    Intramembranei225 – 23171 Publication
    Topological domaini232 – 24312Extracellular1 PublicationAdd
    BLAST
    Transmembranei244 – 26724Helical1 PublicationAdd
    BLAST
    Topological domaini268 – 33669Cytoplasmic1 PublicationAdd
    BLAST

    GO - Cellular componenti

    1. apical plasma membrane Source: Ensembl
    2. brush border membrane Source: Ensembl
    3. endosome Source: Ensembl
    4. plasma membrane Source: Reactome
    5. voltage-gated potassium channel complex Source: ProtInc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi161 – 1611T → A: No effect on channel activity. 1 Publication

    Organism-specific databases

    PharmGKBiPA219.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Potassium channel subfamily K member 1PRO_0000101740Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi69 – 69Interchain1 Publication
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiO00180.
    PaxDbiO00180.
    PRIDEiO00180.

    PTM databases

    PhosphoSiteiO00180.

    Expressioni

    Tissue specificityi

    Widely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney (PubMed:8605869).1 Publication

    Gene expression databases

    BgeeiO00180.
    CleanExiHS_KCNK1.
    ExpressionAtlasiO00180. baseline and differential.
    GenevestigatoriO00180.

    Organism-specific databases

    HPAiCAB022588.
    HPA016049.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked.1 Publication

    Protein-protein interaction databases

    BioGridi109976. 5 interactions.
    DIPiDIP-59532N.
    IntActiO00180. 1 interaction.
    STRINGi9606.ENSP00000355580.

    Structurei

    Secondary structure

    1
    336
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi19 – 6648Combined sources
    Helixi72 – 8615Combined sources
    Turni87 – 893Combined sources
    Helixi104 – 11512Combined sources
    Helixi128 – 16033Combined sources
    Turni163 – 1675Combined sources
    Helixi177 – 19519Combined sources
    Helixi197 – 20610Combined sources
    Beta strandi207 – 2093Combined sources
    Helixi212 – 22312Combined sources
    Beta strandi236 – 2383Combined sources
    Helixi242 – 26827Combined sources
    Helixi271 – 2788Combined sources

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3UKMX-ray3.40A/B/C/D19-288[»]
    ProteinModelPortaliO00180.
    SMRiO00180. Positions 19-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni117 – 1226Selectivity filter 11 Publication
    Regioni225 – 2306Selectivity filter 21 Publication

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00760000118858.
    HOGENOMiHOG000286014.
    HOVERGENiHBG052237.
    InParanoidiO00180.
    KOiK04912.
    OMAiKQLRKMF.
    OrthoDBiEOG7TTQ85.
    PhylomeDBiO00180.
    TreeFamiTF313947.

    Family and domain databases

    InterProiIPR003280. 2pore_dom_K_chnl.
    IPR013099. 2pore_dom_K_chnl_dom.
    IPR003092. 2pore_dom_K_chnl_TASK.
    IPR005408. 2pore_dom_K_chnl_TWIK.
    IPR001779. 2pore_dom_K_chnl_TWIK1.
    [Graphical view]
    PfamiPF07885. Ion_trans_2. 2 hits.
    [Graphical view]
    PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
    PRINTSiPR01333. 2POREKCHANEL.
    PR01096. TWIK1CHANNEL.
    PR01586. TWIKCHANNEL.

    Sequencei

    Sequence statusi: Complete.

    O00180-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED
    60 70 80 90 100
    LLRQELRKLK RRFLEEHECL SEQQLEQFLG RVLEASNYGV SVLSNASGNW
    110 120 130 140 150
    NWDFTSALFF ASTVLSTTGY GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT
    160 170 180 190 200
    AVVQRITVHV TRRPVLYFHI RWGFSKQVVA IVHAVLLGFV TVSCFFFIPA
    210 220 230 240 250
    AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK FRELYKIGIT
    260 270 280 290 300
    CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDQ VHIIEHDQLS
    310 320 330
    FSSITDQAAG MKEDQKQNEP FVATQSSACV DGPANH
    Length:336
    Mass (Da):38,143
    Last modified:July 1, 1997 - v1
    Checksum:i2A41D9501323215D
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U33632 mRNA. Translation: AAB01688.1.
    U76996 mRNA. Translation: AAB97878.1.
    U90065 mRNA. Translation: AAB51147.1.
    AL356357 Genomic DNA. Translation: CAI21792.1.
    CH471098 Genomic DNA. Translation: EAW69989.1.
    BC018051 mRNA. Translation: AAH18051.1.
    CCDSiCCDS1599.1.
    PIRiS65566.
    RefSeqiNP_002236.1. NM_002245.3.
    UniGeneiHs.208544.

    Genome annotation databases

    EnsembliENST00000366621; ENSP00000355580; ENSG00000135750.
    GeneIDi3775.
    KEGGihsa:3775.
    UCSCiuc010pxo.1. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U33632 mRNA. Translation: AAB01688.1.
    U76996 mRNA. Translation: AAB97878.1.
    U90065 mRNA. Translation: AAB51147.1.
    AL356357 Genomic DNA. Translation: CAI21792.1.
    CH471098 Genomic DNA. Translation: EAW69989.1.
    BC018051 mRNA. Translation: AAH18051.1.
    CCDSiCCDS1599.1.
    PIRiS65566.
    RefSeqiNP_002236.1. NM_002245.3.
    UniGeneiHs.208544.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3UKMX-ray3.40A/B/C/D19-288[»]
    ProteinModelPortaliO00180.
    SMRiO00180. Positions 19-281.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi109976. 5 interactions.
    DIPiDIP-59532N.
    IntActiO00180. 1 interaction.
    STRINGi9606.ENSP00000355580.

    Chemistry

    DrugBankiDB00308. Ibutilide.
    DB00908. Quinidine.
    DB01346. Quinidine barbiturate.

    PTM databases

    PhosphoSiteiO00180.

    Proteomic databases

    MaxQBiO00180.
    PaxDbiO00180.
    PRIDEiO00180.

    Protocols and materials databases

    DNASUi3775.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000366621; ENSP00000355580; ENSG00000135750.
    GeneIDi3775.
    KEGGihsa:3775.
    UCSCiuc010pxo.1. human.

    Organism-specific databases

    CTDi3775.
    GeneCardsiGC01P233750.
    HGNCiHGNC:6272. KCNK1.
    HPAiCAB022588.
    HPA016049.
    MIMi601745. gene.
    neXtProtiNX_O00180.
    PharmGKBiPA219.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiCOG1226.
    GeneTreeiENSGT00760000118858.
    HOGENOMiHOG000286014.
    HOVERGENiHBG052237.
    InParanoidiO00180.
    KOiK04912.
    OMAiKQLRKMF.
    OrthoDBiEOG7TTQ85.
    PhylomeDBiO00180.
    TreeFamiTF313947.

    Enzyme and pathway databases

    ReactomeiREACT_75779. Tandem of pore domain in a weak inwardly rectifying K+ channels (TWIK).

    Miscellaneous databases

    ChiTaRSiKCNK1. human.
    GeneWikiiKCNK1.
    GenomeRNAii3775.
    NextBioi14807.
    PROiO00180.
    SOURCEiSearch...

    Gene expression databases

    BgeeiO00180.
    CleanExiHS_KCNK1.
    ExpressionAtlasiO00180. baseline and differential.
    GenevestigatoriO00180.

    Family and domain databases

    InterProiIPR003280. 2pore_dom_K_chnl.
    IPR013099. 2pore_dom_K_chnl_dom.
    IPR003092. 2pore_dom_K_chnl_TASK.
    IPR005408. 2pore_dom_K_chnl_TWIK.
    IPR001779. 2pore_dom_K_chnl_TWIK1.
    [Graphical view]
    PfamiPF07885. Ion_trans_2. 2 hits.
    [Graphical view]
    PIRSFiPIRSF038061. K_channel_subfamily_K_type. 1 hit.
    PRINTSiPR01333. 2POREKCHANEL.
    PR01096. TWIK1CHANNEL.
    PR01586. TWIKCHANNEL.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure."
      Lesage F., Guillemare E., Fink M., Duprat F., Lazdunski M., Romey G., Barhanin J.
      EMBO J. 15:1004-1011(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF THR-161, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Tissue: Kidney.
    2. "Sequence and function of the two P domain potassium channels: implications of an emerging superfamily."
      Goldstein S.A.N., Wang K.-W., Ilan N., Pausch M.H.
      J. Mol. Med. 76:13-20(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], REVIEW.
      Tissue: Brain.
    3. "Cloning and localization of a double-pore K channel, KCNK1: exclusive expression in distal nephron segments."
      Orias M., Velazquez H., Tung F., Lee G., Desir G.V.
      Am. J. Physiol. 273:F663-F666(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "The DNA sequence and biological annotation of human chromosome 1."
      Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.
      , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
      Nature 441:315-321(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Crystal structure of the human two-pore domain potassium channel K2P1."
      Miller A.N., Long S.B.
      Science 335:432-436(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 23-288 IN COMPLEX WITH POTASSIUM IONS, FUNCTION, SUBUNIT, DISULFIDE BOND, TOPOLOGY, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiKCNK1_HUMAN
    AccessioniPrimary (citable) accession number: O00180
    Secondary accession number(s): Q13307, Q5T5E8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: July 1, 1997
    Last modified: April 1, 2015
    This is version 138 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.