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O00180 (KCNK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel subfamily K member 1
Alternative name(s):
Inward rectifying potassium channel protein TWIK-1
Potassium channel KCNO1
Gene names
Name:KCNK1
Synonyms:HOHO1, KCNO1, TWIK1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length336 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Weakly inward rectifying potassium channel. Ref.1

Subunit structure

Homodimer; disulfide-linked. Ref.8

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Widely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney.

Miscellaneous

Inhibited by barium, quinine, quinidine and internal acidification. Activated by protein kinase C.

Sequence similarities

Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 336336Potassium channel subfamily K member 1
PRO_0000101740

Regions

Topological domain1 – 2020Cytoplasmic Potential
Transmembrane21 – 4121Helical; Potential
Intramembrane104 – 13027Pore-forming; Name=Pore-forming 1; Potential
Transmembrane133 – 15321Helical; Potential
Topological domain154 – 17724Cytoplasmic Potential
Transmembrane178 – 19821Helical; Potential
Intramembrane212 – 23827Pore-forming; Name=Pore-forming 2; Potential
Transmembrane247 – 26721Helical; Potential
Topological domain268 – 33669Cytoplasmic Potential

Amino acid modifications

Glycosylation951N-linked (GlcNAc...) Potential
Disulfide bond69Interchain Ref.8

Experimental info

Mutagenesis1611T → A: No effect on channel activity. Ref.1

Secondary structure

......................... 336
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O00180 [UniParc].

Last modified July 1, 1997. Version 1.
Checksum: 2A41D9501323215D

FASTA33638,143
        10         20         30         40         50         60 
MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED LLRQELRKLK 

        70         80         90        100        110        120 
RRFLEEHECL SEQQLEQFLG RVLEASNYGV SVLSNASGNW NWDFTSALFF ASTVLSTTGY 

       130        140        150        160        170        180 
GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT AVVQRITVHV TRRPVLYFHI RWGFSKQVVA 

       190        200        210        220        230        240 
IVHAVLLGFV TVSCFFFIPA AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK 

       250        260        270        280        290        300 
FRELYKIGIT CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDQ VHIIEHDQLS 

       310        320        330 
FSSITDQAAG MKEDQKQNEP FVATQSSACV DGPANH 

« Hide

References

« Hide 'large scale' references
[1]"TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure."
Lesage F., Guillemare E., Fink M., Duprat F., Lazdunski M., Romey G., Barhanin J.
EMBO J. 15:1004-1011(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF THR-161.
Tissue: Kidney.
[2]"Sequence and function of the two P domain potassium channels: implications of an emerging superfamily."
Goldstein S.A.N., Wang K.-W., Ilan N., Pausch M.H.
J. Mol. Med. 76:13-20(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], REVIEW.
Tissue: Brain.
[3]"Cloning and localization of a double-pore K channel, KCNK1: exclusive expression in distal nephron segments."
Orias M., Velazquez H., Tung F., Lee G., Desir G.V.
Am. J. Physiol. 273:F663-F666(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[7]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Crystal structure of the human two-pore domain potassium channel K2P1."
Miller A.N., Long S.B.
Science 335:432-436(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 23-288, SUBUNIT, DISULFIDE BOND.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U33632 mRNA. Translation: AAB01688.1.
U76996 mRNA. Translation: AAB97878.1.
U90065 mRNA. Translation: AAB51147.1.
AL356357 Genomic DNA. Translation: CAI21792.1.
CH471098 Genomic DNA. Translation: EAW69989.1.
BC018051 mRNA. Translation: AAH18051.1.
PIRS65566.
RefSeqNP_002236.1. NM_002245.3.
UniGeneHs.208544.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3UKMX-ray3.40A/B/C/D19-288[»]
ProteinModelPortalO00180.
SMRO00180. Positions 19-281.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109976. 4 interactions.
DIPDIP-59532N.
IntActO00180. 1 interaction.
STRING9606.ENSP00000355580.

Chemistry

DrugBankDB00308. Ibutilide.
DB00908. Quinidine.

PTM databases

PhosphoSiteO00180.

Proteomic databases

PaxDbO00180.
PRIDEO00180.

Protocols and materials databases

DNASU3775.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000366621; ENSP00000355580; ENSG00000135750.
GeneID3775.
KEGGhsa:3775.
UCSCuc010pxo.1. human.

Organism-specific databases

CTD3775.
GeneCardsGC01P233750.
HGNCHGNC:6272. KCNK1.
HPACAB022588.
HPA016049.
MIM601745. gene.
neXtProtNX_O00180.
PharmGKBPA219.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1226.
HOGENOMHOG000286014.
HOVERGENHBG052237.
InParanoidO00180.
KOK04912.
OMAKQLRKMF.
OrthoDBEOG7TTQ85.
PhylomeDBO00180.
TreeFamTF313947.

Enzyme and pathway databases

ReactomeREACT_13685. Neuronal System.

Gene expression databases

ArrayExpressO00180.
BgeeO00180.
CleanExHS_KCNK1.
GenevestigatorO00180.

Family and domain databases

InterProIPR003280. 2pore_dom_K_chnl.
IPR013099. 2pore_dom_K_chnl_dom.
IPR003092. 2pore_dom_K_chnl_TASK.
IPR005408. 2pore_dom_K_chnl_TWIK.
IPR001779. 2pore_dom_K_chnl_TWIK1.
[Graphical view]
PfamPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PIRSFPIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTSPR01333. 2POREKCHANEL.
PR01096. TWIK1CHANNEL.
PR01586. TWIKCHANNEL.
ProtoNetSearch...

Other

ChiTaRSKCNK1. human.
GeneWikiKCNK1.
GenomeRNAi3775.
NextBio14807.
PROO00180.
SOURCESearch...

Entry information

Entry nameKCNK1_HUMAN
AccessionPrimary (citable) accession number: O00180
Secondary accession number(s): Q13307, Q5T5E8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: July 1, 1997
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM