O00180 (KCNK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 1

Alternative name(s):
Inward rectifying potassium channel protein TWIK-1
Potassium channel KCNO1

Gene names

Name:	KCNK1
Synonyms:	HOHO1, KCNO1, TWIK1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Weakly inward rectifying potassium channel. Ref.1
Subunit structure	Homodimer Potential.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Widely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney.
Miscellaneous	Inhibited by barium, quinine, quinidine and internal acidification. Activated by protein kinase C.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ionic channel Potassium channel Voltage-gated channel
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	synaptic transmission Traceable author statement. Source: Reactome
Cellular component	voltage-gated potassium channel complex Traceable author statement. Source: ProtInc
Molecular function	inward rectifier potassium channel activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	Potassium channel subfamily K member 1		PRO_0000101740
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Helical; Potential
	Intramembrane	104 – 130	27	Pore-forming; Name=Pore-forming 1; Potential
	Transmembrane	133 – 153	21	Helical; Potential
	Topological domain	154 – 177	24	Cytoplasmic Potential
	Transmembrane	178 – 198	21	Helical; Potential
	Intramembrane	212 – 238	27	Pore-forming; Name=Pore-forming 2; Potential
	Transmembrane	247 – 267	21	Helical; Potential
	Topological domain	268 – 336	69	Cytoplasmic Potential
Amino acid modifications
	Modified residue	326	1	Phosphoserine By similarity
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	161	1	T → A: No effect on channel activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O00180 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 2A41D9501323215D	FASTA	336	38,143
	10 20 30 40 50 60 MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED LLRQELRKLK 70 80 90 100 110 120 RRFLEEHECL SEQQLEQFLG RVLEASNYGV SVLSNASGNW NWDFTSALFF ASTVLSTTGY 130 140 150 160 170 180 GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT AVVQRITVHV TRRPVLYFHI RWGFSKQVVA 190 200 210 220 230 240 IVHAVLLGFV TVSCFFFIPA AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK 250 260 270 280 290 300 FRELYKIGIT CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDQ VHIIEHDQLS 310 320 330 FSSITDQAAG MKEDQKQNEP FVATQSSACV DGPANH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure." Lesage F., Guillemare E., Fink M., Duprat F., Lazdunski M., Romey G., Barhanin J. EMBO J. 15:1004-1011(1996) [PubMed: 8605869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF THR-161. Tissue: Kidney.
[2]	"Sequence and function of the two P domain potassium channels: implications of an emerging superfamily." Goldstein S.A.N., Wang K.-W., Ilan N., Pausch M.H. J. Mol. Med. 76:13-20(1998) [PubMed: 9462864] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], REVIEW. Tissue: Brain.
[3]	"Cloning and localization of a double-pore K channel, KCNK1: exclusive expression in distal nephron segments." Orias M., Velazquez H., Tung F., Lee G., Desir G.V. Am. J. Physiol. 273:F663-F666(1997) [PubMed: 9362344] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U33632 mRNA. Translation: AAB01688.1. U76996 mRNA. Translation: AAB97878.1. U90065 mRNA. Translation: AAB51147.1. AL356357 Genomic DNA. Translation: CAI21792.1. CH471098 Genomic DNA. Translation: EAW69989.1. BC018051 mRNA. Translation: AAH18051.1.
IPI	IPI00010476.
PIR	S65566.
RefSeq	NP_002236.1. NM_002245.3.
UniGene	Hs.208544.
3D structure databases
ProteinModelPortal	O00180.
SMR	O00180. Positions 66-159, 195-270.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00180. 1 interaction.
STRING	O00180.
PTM databases
PhosphoSite	O00180.
Proteomic databases
PRIDE	O00180.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000366621; ENSP00000355580; ENSG00000135750.
GeneID	3775.
KEGG	hsa:3775.
UCSC	uc001hvv.1. human.
Organism-specific databases
CTD	3775.
GeneCards	GC01P233750.
H-InvDB	HIX0001706.
HGNC	HGNC:6272. KCNK1.
HPA	CAB022588. HPA016049.
MIM	601745. gene.
neXtProt	NX_O00180.
PharmGKB	PA219.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17808.
GeneTree	ENSGT00600000084099.
HOGENOM	HBG446730.
HOVERGEN	HBG052237.
InParanoid	O00180.
OMA	HECLSEP.
OrthoDB	EOG451DRF.
PhylomeDB	O00180.
Enzyme and pathway databases
Reactome	REACT_13685. Neuronal System.
Gene expression databases
ArrayExpress	O00180.
Bgee	O00180.
CleanEx	HS_KCNK1.
Genevestigator	O00180.
GermOnline	ENSG00000135750. Homo sapiens.
Family and domain databases
InterPro	IPR003280. 2pore_dom_K_chnl. IPR022306. 2pore_dom_K_chnl_TASK/TWIK. IPR005408. 2pore_dom_K_chnl_TWIK. IPR001779. 2pore_dom_K_chnl_TWIK1. IPR013099. Ion_trans_2. [Graphical view]
KO	K04912.
PANTHER	PTHR11003:SF27. TWIK1_channel. 1 hit.
Pfam	PF07885. Ion_trans_2. 2 hits. [Graphical view]
PIRSF	PIRSF038061. K_channel_subfamily_K_type. 1 hit.
PRINTS	PR01333. 2POREKCHANEL. PR01096. TWIK1CHANNEL. PR01586. TWIKCHANNEL.
ProtoNet	Search...
Other
DrugBank	DB00308. Ibutilide. DB00908. Quinidine.
NextBio	14807.
SOURCE	Search...

Entry information

Entry name

KCNK1_HUMAN

Accession

Primary (citable) accession number: O00180
Secondary accession number(s): Q13307, Q5T5E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 1, 1997
Last modified:	January 25, 2012
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.