O00180 (KCNK1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium channel subfamily K member 1

Alternative name(s):
Inward rectifying potassium channel protein TWIK-1
Potassium channel KCNO1

Gene names

Name:	KCNK1
Synonyms:	HOHO1, KCNO1, TWIK1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Weakly inward rectifying potassium channel. Ref.1
Subunit structure	Homodimer; disulfide-linked. Ref.7
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Widely expressed with high levels in heart and brain and lower levels in placenta, lung, liver and kidney.
Miscellaneous	Inhibited by barium, quinine, quinidine and internal acidification. Activated by protein kinase C.
Sequence similarities	Belongs to the two pore domain potassium channel (TC 1.A.1.8) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to nicotine Inferred from electronic annotation. Source: Compara synaptic transmission Traceable author statement. Source: Reactome
Cellular_component	apical plasma membrane Inferred from electronic annotation. Source: Compara brush border membrane Inferred from electronic annotation. Source: Compara endosome Inferred from electronic annotation. Source: Compara voltage-gated potassium channel complex Traceable author statement Ref.1. Source: ProtInc
Molecular_function	inward rectifier potassium channel activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 336

336

Potassium channel subfamily K member 1

PRO_0000101740

Regions

Topological domain

1 – 20

20

Cytoplasmic Potential

Transmembrane

21 – 41

21

Helical; Potential

Intramembrane

104 – 130

27

Pore-forming; Name=Pore-forming 1; Potential

Transmembrane

133 – 153

21

Helical; Potential

Topological domain

154 – 177

24

Cytoplasmic Potential

Transmembrane

178 – 198

21

Helical; Potential

Intramembrane

212 – 238

27

Pore-forming; Name=Pore-forming 2; Potential

Transmembrane

247 – 267

21

Helical; Potential

Topological domain

268 – 336

69

Cytoplasmic Potential

Amino acid modifications

Modified residue

326

1

Phosphoserine By similarity

Glycosylation

95

1

N-linked (GlcNAc...) Potential

Disulfide bond

69

Interchain Ref.7

Experimental info

Mutagenesis

161

1

T → A: No effect on channel activity. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

336

Helix Strand Turn

Details...

Helix

23 – 66

44

Helix

72 – 86

15

Turn

87 – 89

3

Helix

104 – 115

12

Helix

128 – 160

33

Turn

163 – 167

5

Helix

177 – 195

19

Helix

197 – 206

10

Beta strand

207 – 209

3

Helix

212 – 223

12

Beta strand

236 – 238

3

Helix

242 – 268

27

Helix

271 – 278

8

Sequences

	Sequence		Length	Mass (Da)
	O00180 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 2A41D9501323215D	FASTA	336	38,143
	10 20 30 40 50 60 MLQSLAGSSC VRLVERHRSA WCFGFLVLGY LLYLVFGAVV FSSVELPYED LLRQELRKLK 70 80 90 100 110 120 RRFLEEHECL SEQQLEQFLG RVLEASNYGV SVLSNASGNW NWDFTSALFF ASTVLSTTGY 130 140 150 160 170 180 GHTVPLSDGG KAFCIIYSVI GIPFTLLFLT AVVQRITVHV TRRPVLYFHI RWGFSKQVVA 190 200 210 220 230 240 IVHAVLLGFV TVSCFFFIPA AVFSVLEDDW NFLESFYFCF ISLSTIGLGD YVPGEGYNQK 250 260 270 280 290 300 FRELYKIGIT CYLLLGLIAM LVVLETFCEL HELKKFRKMF YVKKDKDEDQ VHIIEHDQLS 310 320 330 FSSITDQAAG MKEDQKQNEP FVATQSSACV DGPANH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"TWIK-1, a ubiquitous human weakly inward rectifying K+ channel with a novel structure." Lesage F., Guillemare E., Fink M., Duprat F., Lazdunski M., Romey G., Barhanin J. EMBO J. 15:1004-1011(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF THR-161. Tissue: Kidney.
[2]	"Sequence and function of the two P domain potassium channels: implications of an emerging superfamily." Goldstein S.A.N., Wang K.-W., Ilan N., Pausch M.H. J. Mol. Med. 76:13-20(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], REVIEW. Tissue: Brain.
[3]	"Cloning and localization of a double-pore K channel, KCNK1: exclusive expression in distal nephron segments." Orias M., Velazquez H., Tung F., Lee G., Desir G.V. Am. J. Physiol. 273:F663-F666(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[7]	"Crystal structure of the human two-pore domain potassium channel K2P1." Miller A.N., Long S.B. Science 335:432-436(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.4 ANGSTROMS) OF 23-288, SUBUNIT, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U33632 mRNA. Translation: AAB01688.1.
U76996 mRNA. Translation: AAB97878.1.
U90065 mRNA. Translation: AAB51147.1.
AL356357 Genomic DNA. Translation: CAI21792.1.
CH471098 Genomic DNA. Translation: EAW69989.1.
BC018051 mRNA. Translation: AAH18051.1.

IPI

IPI00010476.

PIR

S65566.

RefSeq

NP_002236.1. NM_002245.3.

UniGene

Hs.208544.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3UKM	X-ray	3.40	A/B/C/D	23-288	[»]

ProteinModelPortal

O00180.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-59532N.

IntAct

O00180. 1 interaction.

STRING

9606.ENSP00000355580.

PTM databases

PhosphoSite

O00180.

Proteomic databases

PaxDb

O00180.

PRIDE

O00180.

Protocols and materials databases

DNASU

3775.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000366621; ENSP00000355580; ENSG00000135750.

GeneID

3775.

KEGG

hsa:3775.

UCSC

uc010pxo.1. human.

Organism-specific databases

CTD

3775.

GeneCards

GC01P233750.

HGNC

HGNC:6272. KCNK1.

HPA

CAB022588.
HPA016049.

MIM

601745. gene.

neXtProt

NX_O00180.

PharmGKB

PA219.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1226.

HOGENOM

HOG000286014.

HOVERGEN

HBG052237.

InParanoid

O00180.

KO

K04912.

OMA

KQLRKMF.

OrthoDB

EOG451DRF.

PhylomeDB

O00180.

Enzyme and pathway databases

Reactome

REACT_13685. Neuronal System.

Gene expression databases

ArrayExpress

O00180.

Bgee

O00180.

CleanEx

HS_KCNK1.

Genevestigator

O00180.

GermOnline

ENSG00000135750. Homo sapiens.

Family and domain databases

InterPro

IPR003280. 2pore_dom_K_chnl.
IPR022306. 2pore_dom_K_chnl_TASK/TWIK.
IPR005408. 2pore_dom_K_chnl_TWIK.
IPR001779. 2pore_dom_K_chnl_TWIK1.
IPR013099. Ion_trans_2.
[Graphical view]

PANTHER

PTHR11003:SF27. PTHR11003:SF27. 1 hit.

Pfam

PF07885. Ion_trans_2. 2 hits.
[Graphical view]

PIRSF

PIRSF038061. K_channel_subfamily_K_type. 1 hit.

PRINTS

PR01333. 2POREKCHANEL.
PR01096. TWIK1CHANNEL.
PR01586. TWIKCHANNEL.

ProtoNet

Search...

Other

ChiTaRS

KCNK1. human.

DrugBank

DB00308. Ibutilide.
DB00908. Quinidine.

GenomeRNAi

3775.

NextBio

14807.

SOURCE

Search...

Entry information

Entry name

KCNK1_HUMAN

Accession

Primary (citable) accession number: O00180
Secondary accession number(s): Q13307, Q5T5E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	July 1, 1997
Last modified:	May 1, 2013
This is version 122 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.