O00175 (CCL24_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-C motif chemokine 24

Alternative name(s):
CK-beta-6
Eosinophil chemotactic protein 2
Eotaxin-2
Myeloid progenitor inhibitory factor 2
Short name=MPIF-2
Small-inducible cytokine A24

Gene names

Name:	CCL24
Synonyms:	MPIF2, SCYA24

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3.
Subcellular location	Secreted.
Tissue specificity	Activated monocytes and activated T lymphocytes.
Post-translational modification	N-glycosylated.
Sequence similarities	Belongs to the intercrine beta (chemokine CC) family.

Ontologies

Keywords
Biological process	Chemotaxis Inflammatory response
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cell-cell signaling Traceable author statement Ref.1. Source: ProtInc cytoskeleton organization Inferred from direct assay PubMed 10072545. Source: UniProtKB eosinophil chemotaxis Inferred from direct assay PubMed 10072545. Source: UniProtKB immune response Traceable author statement PubMed 10713092. Source: ProtInc inflammatory response Traceable author statement Ref.1. Source: ProtInc positive regulation of Rac GTPase activity Inferred from direct assay PubMed 19525930. Source: BHF-UCL positive regulation of actin filament polymerization Inferred from direct assay PubMed 19525930. Source: BHF-UCL positive regulation of angiogenesis Inferred from electronic annotation. Source: Compara positive regulation of cell migration Inferred from direct assay PubMed 19525930. Source: BHF-UCL positive regulation of endothelial cell proliferation Inferred from direct assay PubMed 19525930. Source: BHF-UCL positive regulation of inflammatory response Inferred from electronic annotation. Source: Compara regulation of cell shape Inferred from direct assay PubMed 10072545. Source: UniProtKB signal transduction Traceable author statement Ref.1. Source: ProtInc
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	chemokine activity Inferred from direct assay PubMed 10072545. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 26

26

Ref.1 Ref.2 Ref.7

Chain

27 – 119

93

C-C motif chemokine 24

PRO_0000005232

Amino acid modifications

Glycosylation

115

1

N-linked (GlcNAc...)

Disulfide bond

33 ↔ 58

Disulfide bond

34 ↔ 74

Natural variations

Natural variant

29

1

I → L. Ref.3
Corresponds to variant rs2302006 [ dbSNP | Ensembl ].

VAR_018404

Natural variant

31

1

S → F.
Corresponds to variant rs11465293 [ dbSNP | Ensembl ].

VAR_048710

Natural variant

102

1

A → T.
Corresponds to variant rs11465312 [ dbSNP | Ensembl ].

VAR_048711

Natural variant

110

1

Q → E.
Corresponds to variant rs11465313 [ dbSNP | Ensembl ].

VAR_048712

Experimental info

Sequence conflict

61

1

A → G in AAB51135. Ref.1

Sequence conflict

73

1

F → S AA sequence Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

119

Helix Strand Turn

Details...

Beta strand

35 – 37

3

Turn

44 – 46

3

Beta strand

47 – 52

6

Beta strand

56 – 60

5

Beta strand

63 – 67

5

Beta strand

73 – 75

3

Helix

80 – 92

13

Sequences

	Sequence		Length	Mass (Da)
	O00175 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 6CAACA61731FB393	FASTA	119	13,134
	10 20 30 40 50 60 MAGLMTIVTS LLFLGVCAHH IIPTGSVVIP SPCCMFFVSK RIPENRVVSY QLSSRSTCLK 70 80 90 100 110 AGVIFTTKKG QQFCGDPKQE WVQRYMKNLD AKQKKASPRA RAVAVKGPVQ RYPGNQTTC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and functional characterization of two novel human C-C chemokines as inhibitors of two distinct classes of myeloid progenitors." Patel V.P., Kreider B.L., Li Y., Li H., Leung K., Salcedo T., Nardelli B., Pippalla V., Gentz S., Thotakura R., Parmelee D., Gentz R., Garotta G. J. Exp. Med. 185:1163-1172(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-41 AND 73. Tissue: Monocyte.
[2]	"Cloning and functional characterization of a novel human CC chemokine that binds to the CCR3 receptor and activates human eosinophils." White J.R., Imburgia C., Dul E., Appelbaum E., O'Donnell K., O'Shannessy D.J., Brawner M., Fornwald J., Adamou J., Elshourbagy N.A., Kaiser K., Foley J.J., Schmidt D.B., Johanson K., Macphee C., Moores K., McNulty D., Scott G.F., Schleimer R.P., Sarau H.M. J. Leukoc. Biol. 62:667-675(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS. Tissue: Monocyte.
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-29. Tissue: Colon.
[4]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"cDNA, genomic organisation and chromosomal location of the MPIF-2 (eotaxin-2) gene." Hein H., Theran L. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3-117.
[7]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 27-41.
[8]	"NMR solution structure and receptor peptide binding of the CC chemokine eotaxin-2." Mayer K.L., Stone M.J. Biochemistry 39:8382-8395(2000) [PubMed] [Europe PMC] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Web resources

Wikipedia

CCL24 entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U85768 mRNA. Translation: AAB51135.1.
AK312216 mRNA. Translation: BAG35149.1.
AC005102 Genomic DNA. Translation: AAD15410.1.
BC069072 mRNA. Translation: AAH69072.1.
BC069391 mRNA. Translation: AAH69391.1.
AJ223461 mRNA. Translation: CAA11383.1.

IPI

IPI00010462.

RefSeq

NP_002982.2. NM_002991.2.

UniGene

Hs.247838.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1EIG	NMR	-	A	27-99	[»]
1EIH	NMR	-	A	27-99	[»]

ProteinModelPortal

O00175.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-5876N.

STRING

9606.ENSP00000222902.

Proteomic databases

PaxDb

O00175.

PRIDE

O00175.

Protocols and materials databases

DNASU

6369.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000222902; ENSP00000222902; ENSG00000106178.
ENST00000416943; ENSP00000400533; ENSG00000106178.
ENST00000573227; ENSP00000461512; ENSG00000262306.
ENST00000576649; ENSP00000461415; ENSG00000262306.

GeneID

6369.

KEGG

hsa:6369.

UCSC

uc011kga.2. human.

Organism-specific databases

CTD

6369.

GeneCards

GC07M075440.

HGNC

HGNC:10623. CCL24.

MIM

602495. gene.

neXtProt

NX_O00175.

PharmGKB

PA35555.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG41621.

HOGENOM

HOG000036685.

HOVERGEN

HBG017871.

InParanoid

O00175.

KO

K05514.

OMA

MKNLDAK.

OrthoDB

EOG4SN1Q3.

PhylomeDB

O00175.

Gene expression databases

Bgee

O00175.

CleanEx

HS_CCL24.

Genevestigator

O00175.

GermOnline

ENSG00000106178. Homo sapiens.

Family and domain databases

InterPro

IPR001811. Chemokine_IL8-like_dom.
[Graphical view]

Pfam

PF00048. IL8. 1 hit.
[Graphical view]

SMART

SM00199. SCY. 1 hit.
[Graphical view]

SUPFAM

SSF54117. Chemokine_IL8. 1 hit.

PROSITE

PS00472. SMALL_CYTOKINES_CC. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

O00175.

GenomeRNAi

6369.

NextBio

24748.

PMAP-CutDB

O00175.

SOURCE

Search...

Entry information

Entry name

CCL24_HUMAN

Accession

Primary (citable) accession number: O00175
Secondary accession number(s): B2R5K2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.