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O00168

- PLM_HUMAN

UniProt

O00168 - PLM_HUMAN

Protein

Phospholemman

Gene

FXYD1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.

    GO - Molecular functioni

    1. chloride channel activity Source: ProtInc

    GO - Biological processi

    1. cellular calcium ion homeostasis Source: Ensembl
    2. chloride transmembrane transport Source: GOC
    3. chloride transport Source: ProtInc
    4. ion transmembrane transport Source: Reactome
    5. muscle contraction Source: ProtInc
    6. transmembrane transport Source: Reactome

    Keywords - Molecular functioni

    Chloride channel, Ion channel

    Keywords - Biological processi

    Ion transport, Transport

    Keywords - Ligandi

    Chloride

    Enzyme and pathway databases

    ReactomeiREACT_25149. Ion transport by P-type ATPases.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholemman
    Alternative name(s):
    FXYD domain-containing ion transport regulator 1
    Gene namesi
    Name:FXYD1
    Synonyms:PLM
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:4025. FXYD1.

    Subcellular locationi

    GO - Cellular componenti

    1. chloride channel complex Source: UniProtKB-KW
    2. integral component of plasma membrane Source: ProtInc
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi60 – 601C → S: Significantly reduced half-life; when associated with S-62. 1 Publication
    Mutagenesisi62 – 621C → S: Significantly reduced half-life; when associated with S-60. 1 Publication

    Organism-specific databases

    PharmGKBiPA28441.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020By similarityAdd
    BLAST
    Chaini21 – 9272PhospholemmanPRO_0000010359Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi60 – 601S-palmitoyl cysteine1 Publication
    Lipidationi62 – 621S-palmitoyl cysteine1 Publication
    Modified residuei79 – 791PhosphothreonineBy similarity
    Modified residuei83 – 831Phosphoserine; by PKA and PKCBy similarity
    Modified residuei88 – 881Phosphoserine; by PKA2 Publications

    Post-translational modificationi

    Major plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues By similarity. Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK.By similarity
    Palmitoylation increases half-life and stability, it is enhanced upon phosphorylation at Ser-88 by PKA.2 Publications

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiO00168.
    PaxDbiO00168.
    PeptideAtlasiO00168.
    PRIDEiO00168.

    PTM databases

    PhosphoSiteiO00168.

    Expressioni

    Tissue specificityi

    Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.

    Gene expression databases

    BgeeiO00168.
    CleanExiHS_FXYD1.
    GenevestigatoriO00168.

    Organism-specific databases

    HPAiHPA026873.

    Interactioni

    Protein-protein interaction databases

    BioGridi111363. 3 interactions.
    STRINGi9606.ENSP00000343314.

    Structurei

    Secondary structure

    1
    92
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi23 – 264
    Helixi28 – 303
    Helixi34 – 6431
    Turni66 – 683
    Beta strandi69 – 713
    Turni75 – 773
    Helixi80 – 8910

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2J1Imodel-A/B/C/D32-59[»]
    2JO1NMR-A21-92[»]
    ProteinModelPortaliO00168.
    SMRiO00168. Positions 21-92.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO00168.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 3515ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini57 – 9236CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei36 – 5621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FXYD family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG42110.
    HOGENOMiHOG000234467.
    HOVERGENiHBG008212.
    InParanoidiO00168.
    KOiK13360.
    OrthoDBiEOG7WHHDM.
    PhylomeDBiO00168.
    TreeFamiTF333443.

    Family and domain databases

    InterProiIPR000272. Ion-transport_regulator_FXYD.
    [Graphical view]
    PfamiPF02038. ATP1G1_PLM_MAT8. 1 hit.
    [Graphical view]
    ProDomiPD005989. PD005989. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    PROSITEiPS01310. FXYD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O00168-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASLGHILVF CVGLLTMAKA ESPKEHDPFT YDYQSLQIGG LVIAGILFIL   50
    GILIVLSRRC RCKFNQQQRT GEPDEEEGTF RSSIRRLSTR RR 92
    Length:92
    Mass (Da):10,441
    Last modified:August 30, 2002 - v2
    Checksum:i11602EFEAFFD8BD8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31S → P in AAC51286. (PubMed:9169143)Curated
    Sequence conflicti5 – 51G → H in AAC51286. (PubMed:9169143)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U72245 mRNA. Translation: AAC51286.1.
    AK289811 mRNA. Translation: BAF82500.1.
    BC032800 mRNA. Translation: AAH32800.1.
    CCDSiCCDS12445.1.
    RefSeqiNP_001265646.1. NM_001278717.1.
    NP_001265647.1. NM_001278718.1.
    NP_005022.2. NM_005031.4.
    NP_068702.1. NM_021902.3.
    UniGeneiHs.442498.

    Genome annotation databases

    EnsembliENST00000351325; ENSP00000343314; ENSG00000266964.
    ENST00000455515; ENSP00000393611; ENSG00000266964.
    ENST00000588081; ENSP00000467727; ENSG00000266964.
    ENST00000588607; ENSP00000468535; ENSG00000266964.
    ENST00000588715; ENSP00000465289; ENSG00000266964.
    ENST00000589209; ENSP00000466398; ENSG00000266964.
    GeneIDi5348.
    KEGGihsa:5348.
    UCSCiuc002nyc.3. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U72245 mRNA. Translation: AAC51286.1 .
    AK289811 mRNA. Translation: BAF82500.1 .
    BC032800 mRNA. Translation: AAH32800.1 .
    CCDSi CCDS12445.1.
    RefSeqi NP_001265646.1. NM_001278717.1.
    NP_001265647.1. NM_001278718.1.
    NP_005022.2. NM_005031.4.
    NP_068702.1. NM_021902.3.
    UniGenei Hs.442498.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2J1I model - A/B/C/D 32-59 [» ]
    2JO1 NMR - A 21-92 [» ]
    ProteinModelPortali O00168.
    SMRi O00168. Positions 21-92.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111363. 3 interactions.
    STRINGi 9606.ENSP00000343314.

    PTM databases

    PhosphoSitei O00168.

    Proteomic databases

    MaxQBi O00168.
    PaxDbi O00168.
    PeptideAtlasi O00168.
    PRIDEi O00168.

    Protocols and materials databases

    DNASUi 5348.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000351325 ; ENSP00000343314 ; ENSG00000266964 .
    ENST00000455515 ; ENSP00000393611 ; ENSG00000266964 .
    ENST00000588081 ; ENSP00000467727 ; ENSG00000266964 .
    ENST00000588607 ; ENSP00000468535 ; ENSG00000266964 .
    ENST00000588715 ; ENSP00000465289 ; ENSG00000266964 .
    ENST00000589209 ; ENSP00000466398 ; ENSG00000266964 .
    GeneIDi 5348.
    KEGGi hsa:5348.
    UCSCi uc002nyc.3. human.

    Organism-specific databases

    CTDi 5348.
    GeneCardsi GC19P035629.
    HGNCi HGNC:4025. FXYD1.
    HPAi HPA026873.
    MIMi 602359. gene.
    neXtProti NX_O00168.
    PharmGKBi PA28441.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG42110.
    HOGENOMi HOG000234467.
    HOVERGENi HBG008212.
    InParanoidi O00168.
    KOi K13360.
    OrthoDBi EOG7WHHDM.
    PhylomeDBi O00168.
    TreeFami TF333443.

    Enzyme and pathway databases

    Reactomei REACT_25149. Ion transport by P-type ATPases.

    Miscellaneous databases

    EvolutionaryTracei O00168.
    GeneWikii FXYD1.
    GenomeRNAii 5348.
    NextBioi 20726.
    PROi O00168.
    SOURCEi Search...

    Gene expression databases

    Bgeei O00168.
    CleanExi HS_FXYD1.
    Genevestigatori O00168.

    Family and domain databases

    InterProi IPR000272. Ion-transport_regulator_FXYD.
    [Graphical view ]
    Pfami PF02038. ATP1G1_PLM_MAT8. 1 hit.
    [Graphical view ]
    ProDomi PD005989. PD005989. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    PROSITEi PS01310. FXYD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the human and rat phospholemman (PLM) cDNAs and localization of the human PLM gene to chromosome 19q13.1."
      Chen L.-S.K., Lo C.F., Numann R., Cuddy M.
      Genomics 41:435-443(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Heart.
    2. "The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression."
      Sweadner K.J., Rael E.
      Genomics 68:41-56(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain, Lung and Testis.
    5. Cited for: PHOSPHORYLATION BY DMPK.
    6. "The inhibitory effect of phospholemman on the sodium pump requires its palmitoylation."
      Tulloch L.B., Howie J., Wypijewski K.J., Wilson C.R., Bernard W.G., Shattock M.J., Fuller W.
      J. Biol. Chem. 286:36020-36031(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-60 AND CYS-62, PHOSPHORYLATION AT SER-88, MUTAGENESIS OF CYS-60 AND CYS-62.

    Entry informationi

    Entry nameiPLM_HUMAN
    AccessioniPrimary (citable) accession number: O00168
    Secondary accession number(s): A8K196
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: August 30, 2002
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3