O00168 (PLM_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholemman

Alternative name(s):
FXYD domain-containing ion transport regulator 1

Gene names

Name:	FXYD1
Synonyms:	PLM

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May have a functional role in muscle contraction. Induces a hyperpolarization-activated chloride current when exogenously expressed.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Highest expression in skeletal muscle and heart. Moderate levels in brain, placenta, lung, liver, pancreas, uterus, bladder, prostate, small intestine and colon with mucosal lining. Very low levels in kidney, colon and small intestine without mucosa, prostate without endothelial lining, spleen, and testis.
Post-translational modification	Major plasma membrane substrate for cAMP-dependent protein kinase (PK-A) and protein kinase C (PK-C) in several different tissues By similarity. Phosphorylated in response to insulin and adrenergic stimulation. May be phosphorylated by DMPK. Ref.5
Sequence similarities	Belongs to the FXYD family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Domain	Signal Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ionic channel
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	muscle contraction Traceable author statement. Source: ProtInc
Cellular component	chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	chloride channel activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

20

By similarity

Chain

21 – 92

72

Phospholemman

PRO_0000010359

Regions

Topological domain

21 – 35

15

Extracellular Potential

Transmembrane

36 – 56

21

Helical; Potential

Topological domain

57 – 92

36

Cytoplasmic Potential

Amino acid modifications

Modified residue

79

1

Phosphothreonine By similarity

Modified residue

83

1

Phosphoserine; by PKA and PKC By similarity

Modified residue

88

1

Phosphoserine; by PKA By similarity

Experimental info

Sequence conflict

3

1

S → P in AAC51286. Ref.1

Sequence conflict

5

1

G → H in AAC51286. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

92

Helix Strand Turn

Details...

Helix

23 – 26

4

Helix

28 – 30

3

Helix

34 – 64

31

Turn

66 – 68

3

Beta strand

69 – 71

3

Turn

75 – 77

3

Helix

80 – 89

10

Sequences

	Sequence		Length	Mass (Da)
	O00168 [UniParc]. Last modified August 30, 2002. Version 2. Checksum: 11602EFEAFFD8BD8	FASTA	92	10,441
	10 20 30 40 50 60 MASLGHILVF CVGLLTMAKA ESPKEHDPFT YDYQSLQIGG LVIAGILFIL GILIVLSRRC 70 80 90 RCKFNQQQRT GEPDEEEGTF RSSIRRLSTR RR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the human and rat phospholemman (PLM) cDNAs and localization of the human PLM gene to chromosome 19q13.1." Chen L.-S.K., Lo C.F., Numann R., Cuddy M. Genomics 41:435-443(1997) [PubMed: 9169143] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[2]	"The FXYD gene family of small ion transport regulators or channels: cDNA sequence, protein signature sequence, and expression." Sweadner K.J., Rael E. Genomics 68:41-56(2000) [PubMed: 10950925] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Lung and Testis.
[5]	"Phospholemman is a substrate for myotonic dystrophy protein kinase." Mounsey J.P., John J.E. III, Helmke S.M., Bush E.W., Gilbert J., Roses A.D., Perryman M.B., Jones L.R., Moorman J.R. J. Biol. Chem. 275:23362-23367(2000) [PubMed: 10811636] [Abstract] Cited for: PHOSPHORYLATION BY DMPK.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U72245 mRNA. Translation: AAC51286.1.
AK289811 mRNA. Translation: BAF82500.1.
BC032800 mRNA. Translation: AAH32800.1.

IPI

IPI00010442.

RefSeq

NP_005022.2. NM_005031.3.
NP_068702.1. NM_021902.2.

UniGene

Hs.442498.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2J1I	model	-	A/B/C/D	32-59	[»]
2JO1	NMR	-	A	21-92	[»]

ProteinModelPortal

O00168.

SMR

O00168. Positions 21-92.

ModBase

Search...

Protein-protein interaction databases

STRING

O00168.

PTM databases

PhosphoSite

O00168.

Proteomic databases

PeptideAtlas

O00168.

PRIDE

O00168.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000351325; ENSP00000343314; ENSG00000221857.
ENST00000455515; ENSP00000393611; ENSG00000221857.

GeneID

5348.

KEGG

hsa:5348.

UCSC

uc002nyc.1. human.

Organism-specific databases

CTD

5348.

GeneCards

GC19P035629.

H-InvDB

HIX0202855.

HGNC

HGNC:4025. FXYD1.

HPA

HPA026873.

MIM

602359. gene.

neXtProt

NX_O00168.

PharmGKB

PA28441.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG20479.

GeneTree

ENSGT00530000063932.

HOGENOM

HBG444253.

HOVERGEN

HBG008212.

InParanoid

O00168.

OMA

RKCRCKF.

OrthoDB

EOG4PG62F.

PhylomeDB

O00168.

Gene expression databases

ArrayExpress

O00168.

Bgee

O00168.

CleanEx

HS_FXYD1.

Genevestigator

O00168.

GermOnline

ENSG00000126258. Homo sapiens.

Family and domain databases

InterPro

IPR000272. Ion-transport_regulator_FXYD.
[Graphical view]

KO

K13360.

Pfam

PF02038. ATP1G1_PLM_MAT8. 1 hit.
[Graphical view]

ProDom

PD005989. PD005989. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS01310. FXYD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

20726.

SOURCE

Search...

Entry information

Entry name

PLM_HUMAN

Accession

Primary (citable) accession number: O00168
Secondary accession number(s): A8K196

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	August 30, 2002
Last modified:	January 25, 2012
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.