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Protein

Synaptosomal-associated protein 23

Gene

SNAP23

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • syntaxin binding Source: UniProtKB

GO - Biological processi

  • histamine secretion by mast cell Source: UniProtKB
  • membrane fusion Source: ProtInc
  • post-Golgi vesicle-mediated transport Source: Reactome
  • protein transport Source: UniProtKB-KW
  • synaptic vesicle fusion to presynaptic active zone membrane Source: GO_Central
  • synaptic vesicle priming Source: GO_Central
  • vesicle targeting Source: ProtInc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-1236974. ER-Phagosome pathway.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-421837. Clathrin derived vesicle budding.
R-HSA-6798695. Neutrophil degranulation.
SIGNORiO00161.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Synaptosomal-associated protein 23
Short name:
SNAP-23
Alternative name(s):
Vesicle-membrane fusion protein SNAP-23
Gene namesi
Name:SNAP23
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:11131. SNAP23.

Subcellular locationi

GO - Cellular componenti

  • azurophil granule Source: UniProtKB
  • cell-cell junction Source: BHF-UCL
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • focal adhesion Source: UniProtKB
  • mast cell granule Source: GOC
  • neuron projection Source: UniProtKB-SubCell
  • nucleoplasm Source: HPA
  • plasma membrane Source: UniProtKB
  • presynapse Source: GOC
  • SNARE complex Source: UniProtKB
  • specific granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi8773.
OpenTargetsiENSG00000092531.
PharmGKBiPA35979.

Polymorphism and mutation databases

BioMutaiSNAP23.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002135981 – 211Synaptosomal-associated protein 23Add BLAST211

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources2 Publications1
Modified residuei5PhosphoserineCombined sources1
Modified residuei6PhosphoserineCombined sources1
Modified residuei20PhosphoserineCombined sources1
Modified residuei23PhosphoserineBy similarity1
Modified residuei34PhosphoserineCombined sources1
Lipidationi79S-palmitoyl cysteine1 Publication1
Lipidationi80S-palmitoyl cysteine1 Publication1
Lipidationi83S-palmitoyl cysteine1 Publication1
Lipidationi85S-palmitoyl cysteine1 Publication1
Lipidationi87S-palmitoyl cysteine1 Publication1
Modified residuei110PhosphoserineCombined sources1
Lipidationi112S-palmitoyl cysteine1 Publication1
Modified residuei161PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

EPDiO00161.
MaxQBiO00161.
PaxDbiO00161.
PeptideAtlasiO00161.
PRIDEiO00161.

2D gel databases

OGPiO00161.

PTM databases

iPTMnetiO00161.
PhosphoSitePlusiO00161.
SwissPalmiO00161.

Miscellaneous databases

PMAP-CutDBO00161.

Expressioni

Tissue specificityi

Ubiquitous. Highest levels where found in placenta.

Gene expression databases

BgeeiENSG00000092531.
CleanExiHS_SNAP23.
ExpressionAtlasiO00161. baseline and differential.
GenevisibleiO00161. HS.

Organism-specific databases

HPAiCAB037083.
CAB037317.
HPA001214.

Interactioni

Subunit structurei

Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3 (PubMed:12556468). Found in a complex with VAMP8 and STX1A (PubMed:12130530). Found in a complex with VAMP8 and STX4 in pancreas (By similarity). Interacts simultaneously with SNAPIN and SYN4 (By similarity). Interacts with STX1A (By similarity). Interacts with STX12 (By similarity). Interacts tightly to multiple syntaxins and synaptobrevins/VAMPs (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats) (By similarity).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NAPAP549202EBI-745000,EBI-749652
NAPBQ9H1153EBI-745000,EBI-3921185
STX11O755585EBI-745000,EBI-714135
VAMP3Q158362EBI-745000,EBI-722343
ZDHHC17Q8IUH54EBI-745000,EBI-524753

GO - Molecular functioni

  • SNAP receptor activity Source: GO_Central
  • syntaxin binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114303. 62 interactors.
IntActiO00161. 30 interactors.
MINTiMINT-4999382.
STRINGi9606.ENSP00000249647.

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 74Combined sources51

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NHLX-ray2.30A23-76[»]
3ZUSX-ray2.95A/B/C/D150-209[»]
ProteinModelPortaliO00161.
SMRiO00161.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO00161.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 76t-SNARE coiled-coil homology 1PROSITE-ProRule annotationAdd BLAST63
Domaini146 – 208t-SNARE coiled-coil homology 2PROSITE-ProRule annotationAdd BLAST63

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 761 PublicationAdd BLAST54

Sequence similaritiesi

Belongs to the SNAP-25 family.Curated
Contains 2 t-SNARE coiled-coil homology domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG3065. Eukaryota.
ENOG410Y3Y0. LUCA.
GeneTreeiENSGT00390000012186.
HOGENOMiHOG000231599.
HOVERGENiHBG056971.
InParanoidiO00161.
KOiK08508.
OMAiDITMRAN.
OrthoDBiEOG091G0N7J.
PhylomeDBiO00161.
TreeFamiTF315125.

Family and domain databases

InterProiIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00835. SNAP-25. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEiPS50192. T_SNARE. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform SNAP-23a (identifier: O00161-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDNLSSEEIQ QRAHQITDES LESTRRILGL AIESQDAGIK TITMLDEQKE
60 70 80 90 100
QLNRIEEGLD QINKDMRETE KTLTELNKCC GLCVCPCNRT KNFESGKAYK
110 120 130 140 150
TTWGDGGENS PCNVVSKQPG PVTNGQLQQP TTGAASGGYI KRITNDARED
160 170 180 190 200
EMEENLTQVG SILGNLKDMA LNIGNEIDAQ NPQIKRITDK ADTNRDRIDI
210
ANARAKKLID S
Length:211
Mass (Da):23,354
Last modified:July 1, 1997 - v1
Checksum:iAC378E9786C3A239
GO
Isoform SNAP-23b (identifier: O00161-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-89: R → S
     90-142: Missing.

Show »
Length:158
Mass (Da):17,789
Checksum:i985F775F40884E07
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti135A → V in AAC50537 (PubMed:8663154).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00618789R → S in isoform SNAP-23b. 1 Publication1
Alternative sequenceiVSP_00618890 – 142Missing in isoform SNAP-23b. 1 PublicationAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55936 mRNA. Translation: AAC50537.1.
Y09567 mRNA. Translation: CAA70760.1.
Y09568 mRNA. Translation: CAA70761.1.
AJ011915 mRNA. Translation: CAA09864.1.
AJ278972, AJ278973, AJ278974 Genomic DNA. Translation: CAC07504.1.
BT006916 mRNA. Translation: AAP35562.1.
CR457212 mRNA. Translation: CAG33493.1.
BC000148 mRNA. Translation: AAH00148.1.
BC003686 mRNA. Translation: AAH03686.1.
BC022890 mRNA. Translation: AAH22890.1.
CCDSiCCDS10087.1. [O00161-1]
CCDS10088.1. [O00161-2]
PIRiJC5296.
JC5297.
RefSeqiNP_003816.2. NM_003825.3. [O00161-1]
NP_570710.1. NM_130798.2. [O00161-2]
UniGeneiHs.511149.

Genome annotation databases

EnsembliENST00000249647; ENSP00000249647; ENSG00000092531. [O00161-1]
ENST00000349777; ENSP00000207062; ENSG00000092531. [O00161-2]
ENST00000397138; ENSP00000380327; ENSG00000092531. [O00161-2]
GeneIDi8773.
KEGGihsa:8773.
UCSCiuc001zpz.3. human. [O00161-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U55936 mRNA. Translation: AAC50537.1.
Y09567 mRNA. Translation: CAA70760.1.
Y09568 mRNA. Translation: CAA70761.1.
AJ011915 mRNA. Translation: CAA09864.1.
AJ278972, AJ278973, AJ278974 Genomic DNA. Translation: CAC07504.1.
BT006916 mRNA. Translation: AAP35562.1.
CR457212 mRNA. Translation: CAG33493.1.
BC000148 mRNA. Translation: AAH00148.1.
BC003686 mRNA. Translation: AAH03686.1.
BC022890 mRNA. Translation: AAH22890.1.
CCDSiCCDS10087.1. [O00161-1]
CCDS10088.1. [O00161-2]
PIRiJC5296.
JC5297.
RefSeqiNP_003816.2. NM_003825.3. [O00161-1]
NP_570710.1. NM_130798.2. [O00161-2]
UniGeneiHs.511149.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NHLX-ray2.30A23-76[»]
3ZUSX-ray2.95A/B/C/D150-209[»]
ProteinModelPortaliO00161.
SMRiO00161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114303. 62 interactors.
IntActiO00161. 30 interactors.
MINTiMINT-4999382.
STRINGi9606.ENSP00000249647.

Protein family/group databases

TCDBi1.F.1.1.1. the synaptosomal vesicle fusion pore (svf-pore) family.

PTM databases

iPTMnetiO00161.
PhosphoSitePlusiO00161.
SwissPalmiO00161.

Polymorphism and mutation databases

BioMutaiSNAP23.

2D gel databases

OGPiO00161.

Proteomic databases

EPDiO00161.
MaxQBiO00161.
PaxDbiO00161.
PeptideAtlasiO00161.
PRIDEiO00161.

Protocols and materials databases

DNASUi8773.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000249647; ENSP00000249647; ENSG00000092531. [O00161-1]
ENST00000349777; ENSP00000207062; ENSG00000092531. [O00161-2]
ENST00000397138; ENSP00000380327; ENSG00000092531. [O00161-2]
GeneIDi8773.
KEGGihsa:8773.
UCSCiuc001zpz.3. human. [O00161-1]

Organism-specific databases

CTDi8773.
DisGeNETi8773.
GeneCardsiSNAP23.
HGNCiHGNC:11131. SNAP23.
HPAiCAB037083.
CAB037317.
HPA001214.
MIMi602534. gene.
neXtProtiNX_O00161.
OpenTargetsiENSG00000092531.
PharmGKBiPA35979.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3065. Eukaryota.
ENOG410Y3Y0. LUCA.
GeneTreeiENSGT00390000012186.
HOGENOMiHOG000231599.
HOVERGENiHBG056971.
InParanoidiO00161.
KOiK08508.
OMAiDITMRAN.
OrthoDBiEOG091G0N7J.
PhylomeDBiO00161.
TreeFamiTF315125.

Enzyme and pathway databases

ReactomeiR-HSA-1236974. ER-Phagosome pathway.
R-HSA-1445148. Translocation of GLUT4 to the plasma membrane.
R-HSA-421837. Clathrin derived vesicle budding.
R-HSA-6798695. Neutrophil degranulation.
SIGNORiO00161.

Miscellaneous databases

ChiTaRSiSNAP23. human.
EvolutionaryTraceiO00161.
GeneWikiiSNAP23.
GenomeRNAii8773.
PMAP-CutDBO00161.
PROiO00161.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000092531.
CleanExiHS_SNAP23.
ExpressionAtlasiO00161. baseline and differential.
GenevisibleiO00161. HS.

Family and domain databases

InterProiIPR000928. SNAP-25.
IPR000727. T_SNARE_dom.
[Graphical view]
PfamiPF00835. SNAP-25. 1 hit.
[Graphical view]
SMARTiSM00397. t_SNARE. 2 hits.
[Graphical view]
PROSITEiPS50192. T_SNARE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNP23_HUMAN
AccessioniPrimary (citable) accession number: O00161
Secondary accession number(s): O00162, Q13602, Q6IAE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 1, 1997
Last modified: November 30, 2016
This is version 176 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.