O00148 (DX39A_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent RNA helicase DDX39A
EC=3.6.4.13

Alternative name(s):
DEAD box protein 39
Nuclear RNA helicase URH49

Gene names

Name:	DDX39A
Synonyms:	DDX39

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in pre-mRNA splicing. Required for the export of mRNA out of the nucleus. Ref.5
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Binds THOC4 and DDX39B/BAT1. Interacts with SARNP. Ref.4 Ref.5 Ref.6
Subcellular location	Nucleus By similarity.
Tissue specificity	Detected in testis, and at lower levels in brain, kidney, lung, thymus, spleen and salivary gland. Ref.5
Induction	Up-regulated in proliferating cells. Present at low levels in quiescent cells. Ref.5
Sequence similarities	Belongs to the DEAD box helicase family. DECD subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.
Sequence caution	The sequence BAB15509.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA export from nucleus Inferred from genetic interaction Ref.5. Source: UniProtKB nuclear mRNA splicing, via spliceosome Inferred from genetic interaction Ref.5. Source: UniProtKB
Cellular component	nucleus Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.5. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 427	426	ATP-dependent RNA helicase DDX39A		PRO_0000055067
Regions
	Domain	75 – 248	174	Helicase ATP-binding
	Domain	260 – 421	162	Helicase C-terminal
	Nucleotide binding	88 – 95	8	ATP By similarity
	Motif	44 – 72	29	Q motif
	Motif	195 – 198	4	DECD box
	Compositional bias	15 – 18	4	Poly-Glu
Amino acid modifications
	Modified residue	2	1	N-acetylalanine Ref.8
	Modified residue	35	1	N6-acetyllysine Ref.9
	Modified residue	40	1	Phosphoserine Ref.7
	Modified residue	171	1	Phosphothreonine Ref.7
	Modified residue	187	1	N6-acetyllysine Ref.9
Natural variations
	Natural variant	142	1	V → I. Corresponds to variant rs36127505 [ dbSNP | Ensembl ].		VAR_052166
Experimental info
	Sequence conflict	38	1	Y → H in BAB15509. Ref.2
	Sequence conflict	64	1	F → L in AAB50231. Ref.1
	Sequence conflict	160	1	L → M in AAB50231. Ref.1
	Sequence conflict	377	1	A → G in AAB50231. Ref.1
	Sequence conflict	401	1	D → H in AAB50231. Ref.1
	Sequence conflict	406	1	F → C in AAB50231. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O00148 [UniParc]. Last modified March 15, 2005. Version 2. Checksum: 38D02D4234995574	FASTA	427	49,130
	10 20 30 40 50 60 MAEQDVENDL LDYDEEEEPQ APQESTPAPP KKDIKGSYVS IHSSGFRDFL LKPELLRAIV 70 80 90 100 110 120 DCGFEHPSEV QHECIPQAIL GMDVLCQAKS GMGKTAVFVL ATLQQIEPVN GQVTVLVMCH 130 140 150 160 170 180 TRELAFQISK EYERFSKYMP SVKVSVFFGG LSIKKDEEVL KKNCPHVVVG TPGRILALVR 190 200 210 220 230 240 NRSFSLKNVK HFVLDECDKM LEQLDMRRDV QEIFRLTPHE KQCMMFSATL SKDIRPVCRK 250 260 270 280 290 300 FMQDPMEVFV DDETKLTLHG LQQYYVKLKD SEKNRKLFDL LDVLEFNQVI IFVKSVQRCM 310 320 330 340 350 360 ALAQLLVEQN FPAIAIHRGM AQEERLSRYQ QFKDFQRRIL VATNLFGRGM DIERVNIVFN 370 380 390 400 410 420 YDMPEDSDTY LHRVARAGRF GTKGLAITFV SDENDAKILN DVQDRFEVNV AELPEEIDIS TYIEQSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Human nuclear RNA helicase." Jelinek W.R. Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[4]	"Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region." Lehner B., Semple J.I., Brown S.E., Counsell D., Campbell R.D., Sanderson C.M. Genomics 83:153-167(2004) [PubMed: 14667819] [Abstract] Cited for: INTERACTION WITH DDX39B.
[5]	"Growth-regulated expression and G0-specific turnover of the mRNA that encodes URH49, a mammalian DExH/D box protein that is highly related to the mRNA export protein UAP56." Pryor A., Tung L., Yang Z., Kapadia F., Chang T.-H., Johnson L.F. Nucleic Acids Res. 32:1857-1865(2004) [PubMed: 15047853] [Abstract] Cited for: FUNCTION, INTERACTION WITH THOC4, INDUCTION, TISSUE SPECIFICITY.
[6]	"Intracellular characterization of DDX39, a novel growth-associated RNA helicase." Sugiura T., Sakurai K., Nagano Y. Exp. Cell Res. 313:782-790(2007) [PubMed: 17196963] [Abstract] Cited for: INTERACTION WITH SARNP.
[7]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-40 AND THR-171, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[8]	"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach." Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S. Anal. Chem. 81:4493-4501(2009) [PubMed: 19413330] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, MASS SPECTROMETRY. Tissue: Embryonic kidney.
[9]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-35 AND LYS-187, MASS SPECTROMETRY.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U90426 mRNA. Translation: AAB50231.1. AK026614 mRNA. Translation: BAB15509.1. Different initiation. BC001009 mRNA. Translation: AAH01009.1.
IPI	IPI00940237.
RefSeq	NP_005795.2. NM_005804.3.
UniGene	Hs.311609.
3D structure databases
ProteinModelPortal	O00148.
SMR	O00148. Positions 45-425.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00148. 8 interactions.
MINT	MINT-1035991.
STRING	O00148.
PTM databases
PhosphoSite	O00148.
Proteomic databases
PeptideAtlas	O00148.
PRIDE	O00148.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000242776; ENSP00000242776; ENSG00000123136. ENST00000451994; ENSP00000388769; ENSG00000123136.
GeneID	10212.
KEGG	hsa:10212.
UCSC	uc002myo.1. human.
Organism-specific databases
CTD	10212.
GeneCards	GC19M014521.
H-InvDB	HIX0014836.
HGNC	HGNC:17821. DDX39A.
neXtProt	NX_O00148.
PharmGKB	PA27226.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17977.
HOVERGEN	HBG107334.
InParanoid	O00148.
OrthoDB	EOG4XSKPX.
PhylomeDB	O00148.
Gene expression databases
ArrayExpress	O00148.
Bgee	O00148.
CleanEx	HS_DDX39.
Genevestigator	O00148.
GermOnline	ENSG00000123136. Homo sapiens.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
KO	K13182.
Pfam	PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	38664.

Entry information

Entry name

DX39A_HUMAN

Accession

Primary (citable) accession number: O00148
Secondary accession number(s): Q9BVP6, Q9H5W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.