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O00107

- PHYA_THIHE

UniProt

O00107 - PHYA_THIHE

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Protein
3-phytase A
Gene
PHYA
Organism
Thielavia heterothallica (Myceliophthora thermophila)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the hydrolysis of inorganic orthophosphate from phytate.

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.

pH dependencei

Optimum pH is 5.5-6.0. Active from 3.5 to 8.5 with phytic acid as substrate. The optimum pH is shifted to more acidic values with 4-nitrophenyl phosphate as substrate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751Nucleophile By similarity
Active sitei369 – 3691Proton donor By similarity

GO - Molecular functioni

  1. 3-phytase activity Source: UniProtKB-EC
  2. acid phosphatase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
3-phytase A (EC:3.1.3.8)
Alternative name(s):
3 phytase A
Myo-inositol hexakisphosphate phosphohydrolase A
Myo-inositol-hexaphosphate 3-phosphohydrolase A
Gene namesi
Name:PHYA
OrganismiThielavia heterothallica (Myceliophthora thermophila)
Taxonomic identifieri78579 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesChaetomiaceaeMyceliophthora

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed prediction
Add
BLAST
Chaini20 – 4874683-phytase A
PRO_0000023973Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi26 ↔ 35 By similarity
Disulfide bondi64 ↔ 421 By similarity
Glycosylationi165 – 1651N-linked (GlcNAc...) Reviewed prediction
Glycosylationi200 – 2001N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi208 ↔ 485 By similarity
Glycosylationi247 – 2471N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi260 ↔ 289 By similarity
Glycosylationi346 – 3461N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi456 ↔ 464 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliO00107.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi423 – 43311Poly-Gly
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.50.1240. 1 hit.
InterProiIPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view]
PfamiPF00328. His_Phos_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMiSSF53254. SSF53254. 1 hit.
PROSITEiPS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O00107-1 [UniParc]FASTAAdd to Basket

« Hide

MTGLGVMVVM VGFLAIASLQ SESRPCDTPD LGFQCGTAIS HFWGQYSPYF    50
SVPSELDASI PDDCEVTFAQ VLSRHGARAP TLKRAASYVD LIDRIHHGAI 100
SYGPGYEFLR TYDYTLGADE LTRTGQQQMV NSGIKFYRRY RALARKSIPF 150
VRTAGQDRVV HSAENFTQGF HSALLADRGS TVRPTLPYDM VVIPETAGAN 200
NTLHNDLCTA FEEGPYSTIG DDAQDTYLST FAGPITARVN ANLPGANLTD 250
ADTVALMDLC PFETVASSSS DPATADAGGG NGRPLSPFCR LFSESEWRAY 300
DYLQSVGKWY GYGPGNPLGP TQGVGFVNEL LARLAGVPVR DGTSTNRTLD 350
GDPRTFPLGR PLYADFSHDN DMMGVLGALG AYDGVPPLDK TARRDPEELG 400
GYAASWAVPF AARIYVEKMR CSGGGGGGGG GEGRQEKDEE MVRVLVNDRV 450
MTLKGCGADE RGMCTLERFI ESMAFARGNG KWDLCFA 487
Length:487
Mass (Da):52,538
Last modified:July 1, 1997 - v1
Checksum:i97D10EDC83D051DB
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59806 Genomic DNA. Translation: AAB52508.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U59806 Genomic DNA. Translation: AAB52508.1 .

3D structure databases

ProteinModelPortali O00107.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.1240. 1 hit.
InterProi IPR000560. His_Pase_superF_clade-2.
IPR029033. His_PPase_superfam.
IPR016274. Histidine_acid_Pase_euk.
[Graphical view ]
Pfami PF00328. His_Phos_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF000894. Acid_phosphatase. 1 hit.
SUPFAMi SSF53254. SSF53254. 1 hit.
PROSITEi PS00616. HIS_ACID_PHOSPHAT_1. 1 hit.
PS00778. HIS_ACID_PHOSPHAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila."
    Mitchell D.B., Vogel K., Weimann B.J., Pasamontes L., van Loon A.P.G.M.
    Microbiology 143:245-252(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.

Entry informationi

Entry nameiPHYA_THIHE
AccessioniPrimary (citable) accession number: O00107
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: July 1, 1997
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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