Reviewed, UniProtKB/Swiss-Prot O00103 (UBC11_SCHPO)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2-20 kDa
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase
    Ubiquitin carrier protein

Gene names

Name:	ubc11
Synonyms:	ubcp4
ORF Names:	SPCC1259.15c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	176 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	G2/M transition of mitotic cell cycle Non-traceable author statement. Source: GeneDB_SPombe cell division Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe cyclin catabolic process Ref.1 Inferred from genetic interaction. Source: GeneDB_SPombe mitotic metaphase/anaphase transition Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe protein ubiquitination Traceable author statement. Source: GeneDB_SPombe regulation of mitotic cell cycle Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Ref.1 Traceable author statement. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	Ubiquitin-conjugating enzyme E2-20 kDa		PRO_0000082566
Sites
	Active site	113	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O00103-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 6C80769C37AD4950	FASTA	176	19,664
	10 20 30 40 50 60 MDSDMQNQNP HTNSKNSSSA GMAVDGHSVT KRLRSELMSL MMSNTPGISA FPDSDSNLLH 70 80 90 100 110 120 WAGTITGPSD TYYEGLKFKI SMSFPANYPY SPPTIIFTSP MWHPNVDMSG NICLDILKDK 130 140 150 160 170 WSAVYNVQTI LLSLQSLLGE PNNASPLNAQ AAELWSKDPI EYKRLLMQRY KEIDEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A ubiquitin-conjugating enzyme in fission yeast that is essential for the onset of anaphase in mitosis." Osaka F., Seino H., Seno T., Yamao F. Mol. Cell. Biol. 17:3388-3397(1997) [PubMed: 9154838] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D85545 Genomic DNA. Translation: BAA20375.1. CU329672 Genomic DNA. Translation: CAB38416.1.
PIR	T40902.
RefSeq	NP_588069.1.
3D structure databases
HSSP	HSSP built from PDB template 2E2C based on UniProtKB Q95044.
ModBase	Search...
Genome annotation databases
GeneID	2539190.
KEGG	spo:SPCC1259.15c.
NMPDR	fig|4896.1.peg.407.
Organism-specific databases
GeneDB_Spombe	SPCC1259.15c.
Phylogenomic databases
OMA	O00103. WANQEEF.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-002391-MON.
BRENDA	6.3.2.19. 653.
Gene expression databases
ArrayExpress	O00103.
Family and domain databases
InterPro	IPR015582. Ubiquitin-conj_enz_E2_H10. IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
PANTHER	PTHR11621:SF26. UbcH10. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBC11_SCHPO

Accession

Primary (citable) accession number: O00103

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)