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Protein

Nitrate reductase

Gene

NIA1

Organism
Phaeosphaeria nodorum (Glume blotch fungus) (Parastagonospora nodorum)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Nitrate reductase is a key enzyme involved in the first step of nitrate assimilation in plants, fungi and bacteria.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseSAAS annotationImported

Keywords - Biological processi

Nitrate assimilationUniRule annotation

Keywords - Ligandi

FADSAAS annotation, Flavoprotein, HemeSAAS annotation, Iron, Metal-binding, MolybdenumSAAS annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductaseUniRule annotation
Gene namesi
Name:NIA1Imported
OrganismiPhaeosphaeria nodorum (Glume blotch fungus) (Parastagonospora nodorum)Imported
Taxonomic identifieri13684 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePhaeosphaeriaceaeParastagonospora

Interactioni

Subunit structurei

Homodimer.SAAS annotation

Protein-protein interaction databases

STRINGi13684.SNOT_06022.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini535 – 61076Cytochrome b5 heme-bindingInterPro annotationAdd
BLAST
Domaini637 – 748112FAD-binding FR-typeInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the nitrate reductase family.UniRule annotation
Contains FAD-binding FR-type domain.SAAS annotation
Contains cytochrome b5 heme-binding domain.SAAS annotation

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O00101-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVAIENKAG VPLPPPTEHQ PQPYAASKSI QLPPSPPETV NGDDSKSNAS
60 70 80 90 100
NSEPDFALPP PTTIPTKTLD VDQKTPDAHV PRDPRLIRLT GVHPFNVEAP
110 120 130 140 150
LTDLFNEGFL TSPELFYVCN HGAVPEVHDS ECLDWEFSVE GLVANPLKIT
160 170 180 190 200
LRQLLEEYEN VTYPITLVCA GNRRKEQNVV RKSKGFQWGP AGLSTALFTG
210 220 230 240 250
VVMKDIIERA KPLKRAKYVC MEGADKLPNG YYGTSVKLNW VMDPNRGIML
260 270 280 290 300
AHGMNGEMLR PDHGKPLRAV IPGQIGGRSV KWLKKLIITA EPSDNWYHIY
310 320 330 340 350
DNRVLPTTVD PDEAAKNPKW WMDDRYAIYD LSPNSAIARP AHDEKLEIKS
360 370 380 390 400
APKSYKVEGY AYSGGGRRIT RCEVSLDKGK TWRLANIDYA EDKYRAHEDR
410 420 430 440 450
ELFGGRLDMD WRETSFCWCF WNLDIAIAEL QETDDIVVRV MDEAMCIQPR
460 470 480 490 500
DMYWSVLGMM NNPWFRIVIH KEGDTLRFEH PTQPALMPGG WMERIKKAGG
510 520 530 540 550
NLTNGYWGER INGQEPEHAA IEDAKEIKMT KDGVDKQISI DELRKHDNAE
560 570 580 590 600
NPWFVVNGEV YDGTAFLEGH PGGAQSIVSV AGLDASDEFM AIHSETAKAM
610 620 630 640 650
MPDYHIGSLD EASRKVLAEG EPVNESTEPR PVFLDARIWN RALLHAKTPV
660 670 680 690 700
SWDTRIFTFK LDHDQQALGL PTGQHLMIRL RDPVTREASS ASYTPISQTS
710 720 730 740 750
KLGFCDVLIK VYADTQEKVG GKMTKALDAI PVGHFVDFKG PIGKFEYLGN
760 770 780 790 800
GRCSINGKQR SVKKLYMVCG GSGITPIFQV LRAVMQDKND PTHCTVLNGN
810 820 830 840 850
RLVEDILCRE DLDVFARENP ERCKLLYTLT KGPEDWEGLR GRIGKELLGE
860 870 880 890
QCKVDSEGSS LVLICGPEAL EKSTHKALNE MGWRDEDLMF F
Length:891
Mass (Da):99,918
Last modified:June 1, 1998 - v2
Checksum:i791CCEC80CD6FB85
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ009827 Genomic DNA. Translation: CAA08857.1.
Y13654 Genomic DNA. Translation: CAA74005.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ009827 Genomic DNA. Translation: CAA08857.1.
Y13654 Genomic DNA. Translation: CAA74005.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi13684.SNOT_06022.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG0534. Eukaryota.
KOG0535. Eukaryota.
KOG0537. Eukaryota.
COG0543. LUCA.
COG2041. LUCA.

Family and domain databases

Gene3Di2.60.40.650. 1 hit.
3.10.120.10. 1 hit.
3.90.420.10. 1 hit.
InterProiIPR001199. Cyt_B5-like_heme/steroid-bd.
IPR018506. Cyt_B5_heme-BS.
IPR017927. Fd_Rdtase_FAD-bd.
IPR014756. Ig_E-set.
IPR005066. MoCF_OxRdtse_dimer.
IPR008335. Mopterin_OxRdtase_euk.
IPR001834. NADH-Cyt_B5_reductase.
IPR012137. Nitr_rd_NADH.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR000572. OxRdtase_Mopterin-bd_dom.
IPR022407. OxRdtase_Mopterin_BS.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00173. Cyt-b5. 1 hit.
PF00970. FAD_binding_6. 1 hit.
PF03404. Mo-co_dimer. 1 hit.
PF00175. NAD_binding_1. 1 hit.
PF00174. Oxidored_molyb. 1 hit.
[Graphical view]
PIRSFiPIRSF000233. Nitr_rd_NADH. 1 hit.
PRINTSiPR00406. CYTB5RDTASE.
PR00363. CYTOCHROMEB5.
PR00407. EUMOPTERIN.
SMARTiSM01117. Cyt-b5. 1 hit.
[Graphical view]
SUPFAMiSSF55856. SSF55856. 1 hit.
SSF56524. SSF56524. 1 hit.
SSF63380. SSF63380. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00191. CYTOCHROME_B5_1. 1 hit.
PS50255. CYTOCHROME_B5_2. 1 hit.
PS51384. FAD_FR. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of the nitrate reductase gene of Stagonospora (Septoria) nodorum and its use as a selectable marker for targeted transformation."
    Cutler S.B., Cooley R.N., Caten C.E.
    Curr. Genet. 34:128-137(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BS171Imported and BSm300Imported.
  2. "Characterisation of the nitrite reductase gene (NII1) and the nitrate-assimilation gene cluster of Stagonospora (Septoria) nodorum."
    Cutler S.B., Caten C.E.
    Curr. Genet. 36:282-289(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: BS171Imported.

Entry informationi

Entry nameiO00101_PHAND
AccessioniPrimary (citable) accession number: O00101
Entry historyi
Integrated into UniProtKB/TrEMBL: July 1, 1997
Last sequence update: June 1, 1998
Last modified: May 11, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.