ID   ADH1_PICST              Reviewed;         348 AA.
AC   O00097; A3LNN7;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Alcohol dehydrogenase 1;
DE            EC=1.1.1.1;
DE   AltName: Full=Alcohol dehydrogenase I;
DE   AltName: Full=ADH 2;
GN   Name=ADH1; Synonyms=ADH2; ORFNames=PICST_68558;
OS   Pichia stipitis (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
OC   Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Pichia.
OX   NCBI_TaxID=4924;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545;
RX   MEDLINE=98207839; PubMed=9546172;
RA   Cho J.Y., Jeffries T.W.;
RT   "Pichia stipitis genes for alcohol dehydrogenase with fermentative and
RT   respiratory functions.";
RL   Appl. Environ. Microbiol. 64:1350-1358(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 62970 / CBS 5774 / NRRL Y-11542;
RX   MEDLINE=99019018; PubMed=9802210;
RX   DOI=10.1002/(SICI)1097-0061(1998100)14:14<1311::AID-YEA315>3.3.CO;2-K;
RA   Passoth V., Schaefer B., Liebel B., Weierstall T., Klinner U.;
RT   "Molecular cloning of alcohol dehydrogenase genes of the yeast Pichia
RT   stipitis and identification of the fermentative ADH.";
RL   Yeast 14:1311-1325(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-
RT   fermenting yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: Converts ethanol to acetaldehyde and plays a major role
CC       in xylose fermentation.
CC   -!- CATALYTIC ACTIVITY: An alcohol + NAD(+) = an aldehyde or ketone +
CC       NADH.
CC   -!- COFACTOR: Binds 2 zinc ions per subunit (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC       family.
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DR   EMBL; AF008245; AAC49991.1; -; Genomic_DNA.
DR   EMBL; Y13397; CAA73827.1; -; Genomic_DNA.
DR   EMBL; CP000496; ABN64893.1; -; Genomic_DNA.
DR   RefSeq; XP_001382922.1; -.
DR   HSSP; P39462; 1JVB.
DR   SMR; O00097; 2-348.
DR   GeneID; 4836752; -.
DR   KEGG; pic:PICST_68558; -.
DR   OMA; O00097; ATDGGPH.
DR   BRENDA; 1.1.1.1; 81766.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004022; F:alcohol dehydrogenase activity; IEA:EC.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013154; ADH_GroES-like.
DR   InterPro; IPR002085; ADH_SF_Zn.
DR   InterPro; IPR013149; ADH_Zn-bd.
DR   InterPro; IPR002328; ADH_Zn_CS.
DR   PANTHER; PTHR11695; ADH_Sf_Zn; 1.
DR   Pfam; PF08240; ADH_N; 1.
DR   Pfam; PF00107; ADH_zinc_N; 1.
DR   PROSITE; PS00059; ADH_ZINC; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Metal-binding; NAD; Oxidoreductase;
KW   Zinc.
FT   CHAIN         1    348       Alcohol dehydrogenase 1.
FT                                /FTId=PRO_0000160727.
FT   NP_BIND     178    184       NAD (By similarity).
FT   NP_BIND     269    271       NAD (By similarity).
FT   METAL        44     44       Zinc 1; catalytic (By similarity).
FT   METAL        67     67       Zinc 1; catalytic (By similarity).
FT   METAL        98     98       Zinc 2 (By similarity).
FT   METAL       101    101       Zinc 2 (By similarity).
FT   METAL       104    104       Zinc 2 (By similarity).
FT   METAL       112    112       Zinc 2 (By similarity).
FT   METAL       154    154       Zinc 1; catalytic (By similarity).
FT   BINDING     202    202       NAD (By similarity).
FT   BINDING     207    207       NAD (By similarity).
FT   BINDING     341    341       NAD (By similarity).
SQ   SEQUENCE   348 AA;  36520 MW;  49C06B545D5350F4 CRC64;
     MSVPTTQKAV VFESNGGPLL YKDIPVPTPK PNEILINVKY SGVCHTDLHA WKGDWPLDTK
     LPLVGGHEGA GVVVGIGSNV TGWELGDYAG IKWLNGSCLN CEFCQHSDEP NCAKADLSGY
     THDGSFQQYA TADAVQAARL PKGTDLAQAA PILCAGITVY KALKTAQIQP GNWVCISGAG
     GGLGSLAIQY AKAMGFRVIA IDGGEEKGEF VKSLGAEAYV DFTVSKDIVK DIQTATDGGP
     HAAINVSVSE KAIAQSCQYV RSTGTVVLVG LPAGAKVVAP VFDAVVKSIS IRGSYVGNRA
     DSAEAIDFFT RGLIKCPIKV VGLSELPKVY ELMEAGKVIG RYVVDTSK
//