Reviewed, UniProtKB/Swiss-Prot O00097 (ADH1_PICST)

Names and origin

Protein names

Recommended name:
    Alcohol dehydrogenase 1
    EC=1.1.1.1
Alternative name(s):
    Alcohol dehydrogenase I
    ADH 2

Gene names

Name:	ADH1
Synonyms:	ADH2
ORF Names:	PICST_68558

Organism

Pichia stipitis (Yeast) [Complete proteome]

Taxonomic identifier

4924 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts ethanol to acetaldehyde and plays a major role in xylose fermentation.
Catalytic activity	An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alcohol dehydrogenase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 348	348	Alcohol dehydrogenase 1		PRO_0000160727
Regions
	Nucleotide binding	178 – 184	7	NAD By similarity
	Nucleotide binding	269 – 271	3	NAD By similarity
Sites
	Metal binding	44	1	Zinc 1; catalytic By similarity
	Metal binding	67	1	Zinc 1; catalytic By similarity
	Metal binding	98	1	Zinc 2 By similarity
	Metal binding	101	1	Zinc 2 By similarity
	Metal binding	104	1	Zinc 2 By similarity
	Metal binding	112	1	Zinc 2 By similarity
	Metal binding	154	1	Zinc 1; catalytic By similarity
	Binding site	202	1	NAD By similarity
	Binding site	207	1	NAD By similarity
	Binding site	341	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O00097-1 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 49C06B545D5350F4	FASTA	348	36,520
	10 20 30 40 50 60 MSVPTTQKAV VFESNGGPLL YKDIPVPTPK PNEILINVKY SGVCHTDLHA WKGDWPLDTK 70 80 90 100 110 120 LPLVGGHEGA GVVVGIGSNV TGWELGDYAG IKWLNGSCLN CEFCQHSDEP NCAKADLSGY 130 140 150 160 170 180 THDGSFQQYA TADAVQAARL PKGTDLAQAA PILCAGITVY KALKTAQIQP GNWVCISGAG 190 200 210 220 230 240 GGLGSLAIQY AKAMGFRVIA IDGGEEKGEF VKSLGAEAYV DFTVSKDIVK DIQTATDGGP 250 260 270 280 290 300 HAAINVSVSE KAIAQSCQYV RSTGTVVLVG LPAGAKVVAP VFDAVVKSIS IRGSYVGNRA 310 320 330 340 DSAEAIDFFT RGLIKCPIKV VGLSELPKVY ELMEAGKVIG RYVVDTSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pichia stipitis genes for alcohol dehydrogenase with fermentative and respiratory functions." Cho J.Y., Jeffries T.W. Appl. Environ. Microbiol. 64:1350-1358(1998) [PubMed: 9546172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.
[2]	"Molecular cloning of alcohol dehydrogenase genes of the yeast Pichia stipitis and identification of the fermentative ADH." Passoth V., Schaefer B., Liebel B., Weierstall T., Klinner U. Yeast 14:1311-1325(1998) [PubMed: 9802210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 62970 / CBS 5774 / NRRL Y-11542.
[3]	"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis." Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A., Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S., Passoth V., Richardson P.M. Nat. Biotechnol. 25:319-326(2007) [PubMed: 17334359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 58785 / CBS 6054 / IFO 10063 / NRRL Y-11545.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF008245 Genomic DNA. Translation: AAC49991.1. Y13397 Genomic DNA. Translation: CAA73827.1. CP000496 Genomic DNA. Translation: ABN64893.1.
RefSeq	XP_001382922.1.
3D structure databases
HSSP	HSSP built from PDB template 1JVB based on UniProtKB P39462.
SMR	O00097. Positions 2-348.
ModBase	Search...
Genome annotation databases
GeneID	4836752.
KEGG	pic:PICST_68558.
Phylogenomic databases
OMA	O00097. ATDGGPH.
Enzyme and pathway databases
BRENDA	1.1.1.1. 81766.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR002328. ADH_Zn_CS. [Graphical view]
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADH1_PICST

Accession

Primary (citable) accession number: O00097
Secondary accession number(s): A3LNN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	July 1, 1997
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)