O00095 (O00095_HYPJE) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Submitted name:
Beta-1,4-glucanase EMBL ABV71388.1
EC=3.2.1.4 EMBL ABV71388.1

Submitted name:
Endo-beta-1,4-glucanase EMBL BAA20140.1
EC=3.2.1.4 EMBL BAA20140.1

Gene names

Name:

egl EMBL BAA20140.1

Organism

Hypocrea jecorina (Trichoderma reesei) EMBL BAA20140.1

Taxonomic identifier

51453 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Hypocreomycetidae › Hypocreales › Hypocreaceae › Hypocrea

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Molecular function	Glycosidase EMBL BAA20140.1 Hydrolase
Technical term	3D-structure PDB 1OA2 PDB 1OLQ PDB 1H8V
Gene Ontology (GO)
Biological process	polysaccharide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Amino acid modifications
	Glycosylation	180	1	N-linked (GlcNAc...) PDB 1OA2 PDB 1OLQ

Sequences

	Sequence		Length	Mass (Da)
	O00095 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: DF476EEDE384ADD1	FASTA	234	25,159
	10 20 30 40 50 60 MKFLQVLPAL IPAALAQTSC DQWATFTGNG YTVSNNLWGA SAGSGFGCVT AVSLSGGASW 70 80 90 100 110 120 HADWQWSGGQ NNVKSYQNSQ IAIPQKRTVN SISSMPTTAS WSYSGSNIRA NVAYDLFTAA 130 140 150 160 170 180 NPNHVTYSGD YELMIWLGKY GDIGPIGSSQ GTVNVGGQSW TLYYGYNGAM QVYSFVAQTN 190 200 210 220 230 TTNYSGDVKN FFNYLRDNKG YNAAGQYVLS YQFGTEPFTG SGTLNVASWT ASIN

References

[1]	"Molecular characterization and heterologous expression of the gene encoding a low-molec ular-mass endoglucanase from Trichoderma reesei QM9414." Okada H., Tada K., Sekiya T., Yokoyama K., Takahashi A., Tohda H., Kumagai H., Morikawa Y. Appl. Environ. Microbiol. 64:55-563(1998) Cited for: NUCLEOTIDE SEQUENCE. Strain: QM9414 EMBL BAA20140.1.
[2]	"The X-ray crystal structure of the Trichoderma reesei family 12 endoglucanase 3, Cel12A, at 1.9 A resolution." Sandgren M., Shaw A., Ropp T.H., Wu S., Bott R., Cameron A.D., Stahlberg J., Mitchinson C., Jones T.A. J. Mol. Biol. 308:295-310(2001) [PubMed: 11327768] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 18-234.
[3]	"Comparison of family 12 glycoside hydrolases and recruited substitutions important for thermal stability." Sandgren M., Gualfetti P.J., Shaw A., Gross L.S., Saldajeno M., Day A.G., Jones T.A., Mitchinson C. Protein Sci. 12:848-860(2003) [PubMed: 12649442] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) OF 18-234, GLYCOSYLATION AT ASN-180.
[4]	"The Humicola grisea Cel12A enzyme structure at 1.2 A resolution and the impact of its free cysteine residues on thermal stability." Sandgren M., Gualfetti P.J., Paech C., Paech S., Shaw A., Gross L.S., Saldajeno M., Berglund G.I., Jones T.A., Mitchinson C. Protein Sci. 12:2782-2793(2003) [PubMed: 14627738] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 18-234, GLYCOSYLATION AT ASN-180.
[5]	"Cloning and characterization of endoglucanase genes from Trichoderma spp." Shaikh Z.J., Bhat S., Kuruvunashetti M.S. Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: IABT1003 EMBL ABV71388.1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

EU149644 Genomic DNA. Translation: ABV71388.1.
AB003694 Genomic DNA. Translation: BAA20140.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1H8V	X-ray	1.90	A/B/C/D/E/F	18-234	[»]
1OA2	X-ray	1.50	A/B/C/D/E/F	18-234	[»]
1OLQ	X-ray	1.70	A/B	18-234	[»]

ProteinModelPortal

O00095.

SMR

O00095. Positions 17-234.

ModBase

Search...

Protein family/group databases

CAZy

GH12. Glycoside Hydrolase Family 12.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Phylogenomic databases

PhylomeDB

O00095.

Enzyme and pathway databases

BRENDA

3.2.1.4. 275907.

Family and domain databases

InterPro

IPR008985. ConA-like_lec_gl.
IPR013319. Glyco_hydro_11/12_cat.
IPR002594. Glyco_hydro_12.
[Graphical view]

Gene3D

G3DSA:2.60.120.180. Glyco_hydro_11/12_cat. 1 hit.

Pfam

PF01670. Glyco_hydro_12. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.

ProtoNet

Search...

Entry information

Entry name

O00095_HYPJE

Accession

Primary (citable) accession number: O00095

Entry history

Integrated into UniProtKB/TrEMBL:	July 1, 1997
Last sequence update:	July 1, 1997
Last modified:	December 14, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)