Reviewed, UniProtKB/Swiss-Prot O00091 (G6PD_SCHPO)

Names and origin

Protein names

Recommended name:
    Glucose-6-phosphate 1-dehydrogenase
      Short name=G6PD
    EC=1.1.1.49

Gene names

Name:	zwf1
ORF Names:	SPAC3A12.18, SPAC9.01

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP+ = D-glucono-1,5-lactone 6-phosphate + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular response to stress Inferred from expression pattern. Source: GeneDB_SPombe glucose 6-phosphate utilization Inferred by curator. Source: GeneDB_SPombe oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Glucose-6-phosphate 1-dehydrogenase		PRO_0000068106
Sites
	Active site	247	1	Proton acceptor By similarity
	Binding site	20	1	NADP By similarity
	Binding site	52	1	NADP By similarity
	Binding site	185	1	Substrate By similarity
	Binding site	189	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	O00091-1 [UniParc]. Last modified January 11, 2001. Version 2. Checksum: 6706DD0847762A22	FASTA	500	57,204
	10 20 30 40 50 60 MSSANLSIKE NGAMVVFGAS GDLSKKKTFP ALFSLFSEGR LPKDIRIVGY ARSKIEHEDF 70 80 90 100 110 120 LDRITQNIKI DEEDSQAKEK LEEFKKRCSY YRGSYDKPED FEGLNSHLCE REGDRSTHNR 130 140 150 160 170 180 IFYLALPPDV FVSVATNLKK KCVPEKGIAR LVIEKPFGVD LKSAQELQSQ LAPLFDEKEI 190 200 210 220 230 240 YRIDHYLGKE MVQNLVHLRF CNPVISHLWD KNSISSVQIT FKEPIGTEGR GGYFDSSTIV 250 260 270 280 290 300 RDIVQNHLVQ ILTLLTMETP TTFSADDLRD EKVKVLRRTR LGDLKDIVLG QYVKSKDGKK 310 320 330 340 350 360 PGYLDDETVP KGSRCPTYSA IPCFIDTERW RGVPFLLKAG KAMDIGKVEI RVQFKAAANG 370 380 390 400 410 420 LFKDAYHNEL VIRVQPDEAI YFKMNIKQPG LSEAPLLTDL DLTYSRRFKN MKLHEAYEAL 430 440 450 460 470 480 FLDAFAGDQS RFARIDELEC AWSLVDPLLK YMEEEKPVPE PYEYGSDGPE CLYSFLKKFG 490 500 YIYDSPDYYD YPVMSVPSDH

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329670 Genomic DNA. Translation: CAB08746.1.
PIR	T39186.
RefSeq	NP_593344.2.
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Protein-protein interaction databases
IntAct	O00091. 1 interaction.
Genome annotation databases
GeneID	2543200.
KEGG	spo:SPAC3A12.18.
Organism-specific databases
GeneDB_Spombe	SPAC3A12.18.
Phylogenomic databases
OMA	O00091. MATADAY.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-001953-MON.
BRENDA	1.1.1.49. 653.
Gene expression databases
ArrayExpress	O00091.
Family and domain databases
InterPro	IPR001282. Glc-6-P_DH. IPR019796. Glc-6-P_DH_AS. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD_SCHPO

Accession

Primary (citable) accession number: O00091
Secondary accession number(s): Q9UT26

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 11, 2001
Last modified:	June 16, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)