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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (zwf1), Probable glucose-6-phosphate 1-dehydrogenase C794.01c (SPCC794.01c), Probable glucose-6-phosphate 1-dehydrogenase C7.13c (SPAC3C7.13c)
  2. Probable 6-phosphogluconolactonase (SPCC16C4.10)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (SPBC660.16)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52NADPBy similarity1
Binding sitei155NADP; via carbonyl oxygenBy similarity1
Binding sitei155SubstrateBy similarity1
Binding sitei223SubstrateBy similarity1
Binding sitei242SubstrateBy similarity1
Active sitei247Proton acceptorBy similarity1
Binding sitei341SubstrateBy similarity1
Binding sitei375SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 25NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-SPO-5628897. TP53 Regulates Metabolic Genes.
R-SPO-71336. Pentose phosphate pathway (hexose monophosphate shunt).
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase (EC:1.1.1.49)
Short name:
G6PD
Gene namesi
Name:zwf1
ORF Names:SPAC3A12.18, SPAC9.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC3A12.18.
PomBaseiSPAC3A12.18. zwf1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000681061 – 500Glucose-6-phosphate 1-dehydrogenaseAdd BLAST500

Proteomic databases

MaxQBiO00091.
PRIDEiO00091.

Interactioni

Protein-protein interaction databases

BioGridi279629. 1 interactor.
IntActiO00091. 1 interactor.
MINTiMINT-4666413.

Structurei

3D structure databases

ProteinModelPortaliO00091.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni185 – 189Substrate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000046192.
InParanoidiO00091.
KOiK00036.
OMAiCDMLVFG.
OrthoDBiEOG092C2MOH.
PhylomeDBiO00091.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O00091-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSANLSIKE NGAMVVFGAS GDLSKKKTFP ALFSLFSEGR LPKDIRIVGY
60 70 80 90 100
ARSKIEHEDF LDRITQNIKI DEEDSQAKEK LEEFKKRCSY YRGSYDKPED
110 120 130 140 150
FEGLNSHLCE REGDRSTHNR IFYLALPPDV FVSVATNLKK KCVPEKGIAR
160 170 180 190 200
LVIEKPFGVD LKSAQELQSQ LAPLFDEKEI YRIDHYLGKE MVQNLVHLRF
210 220 230 240 250
CNPVISHLWD KNSISSVQIT FKEPIGTEGR GGYFDSSTIV RDIVQNHLVQ
260 270 280 290 300
ILTLLTMETP TTFSADDLRD EKVKVLRRTR LGDLKDIVLG QYVKSKDGKK
310 320 330 340 350
PGYLDDETVP KGSRCPTYSA IPCFIDTERW RGVPFLLKAG KAMDIGKVEI
360 370 380 390 400
RVQFKAAANG LFKDAYHNEL VIRVQPDEAI YFKMNIKQPG LSEAPLLTDL
410 420 430 440 450
DLTYSRRFKN MKLHEAYEAL FLDAFAGDQS RFARIDELEC AWSLVDPLLK
460 470 480 490 500
YMEEEKPVPE PYEYGSDGPE CLYSFLKKFG YIYDSPDYYD YPVMSVPSDH
Length:500
Mass (Da):57,204
Last modified:January 11, 2001 - v2
Checksum:i6706DD0847762A22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB08746.1.
PIRiT39186.
RefSeqiNP_593344.2. NM_001018776.2.

Genome annotation databases

EnsemblFungiiSPAC3A12.18.1; SPAC3A12.18.1:pep; SPAC3A12.18.
GeneIDi2543200.
KEGGispo:SPAC3A12.18.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB08746.1.
PIRiT39186.
RefSeqiNP_593344.2. NM_001018776.2.

3D structure databases

ProteinModelPortaliO00091.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279629. 1 interactor.
IntActiO00091. 1 interactor.
MINTiMINT-4666413.

Proteomic databases

MaxQBiO00091.
PRIDEiO00091.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC3A12.18.1; SPAC3A12.18.1:pep; SPAC3A12.18.
GeneIDi2543200.
KEGGispo:SPAC3A12.18.

Organism-specific databases

EuPathDBiFungiDB:SPAC3A12.18.
PomBaseiSPAC3A12.18. zwf1.

Phylogenomic databases

HOGENOMiHOG000046192.
InParanoidiO00091.
KOiK00036.
OMAiCDMLVFG.
OrthoDBiEOG092C2MOH.
PhylomeDBiO00091.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
ReactomeiR-SPO-5628897. TP53 Regulates Metabolic Genes.
R-SPO-71336. Pentose phosphate pathway (hexose monophosphate shunt).

Miscellaneous databases

PROiO00091.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD. 1 hit.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG6PD_SCHPO
AccessioniPrimary (citable) accession number: O00091
Secondary accession number(s): Q9UT26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 11, 2001
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.