O00085 (PHYA1_ASPTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phytase A
EC=3.1.3.8

Alternative name(s):
3 phytase A
Myo-inositol hexakisphosphate phosphohydrolase A
Myo-inositol-hexaphosphate 3-phosphohydrolase A

Gene names

Name:

phyA

Organism

Aspergillus terreus

Taxonomic identifier

33178 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	466 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydrolysis of inorganic orthophosphate from phytate. Releases 5 of the 6 phosphate groups to yield the end product myo-inositol 2-monophosphate. Specific for phytate. Ref.3
Catalytic activity	Myo-inositol hexakisphosphate + H2O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate.
Subunit structure	Monomer. Ref.2
Subcellular location	Secreted.
Sequence similarities	Belongs to the histidine acid phosphatase family.
Biophysicochemical properties	Kinetic parameters: KM=10.6 µM for phytate Ref.3 pH dependence: Optimum pH is 5.5. Active from 2.5 to 7.5 with phytic acid as substrate. The optimum pH is shifted to more acidic values with 4-nitrophenyl phosphate as substrate.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	dephosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-phytase activity Inferred from electronic annotation. Source: EC acid phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 466	447	3-phytase A		PRO_0000023972
Sites
	Active site	82	1	Nucleophile By similarity
	Active site	362	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Glycosylation	339	1	N-linked (GlcNAc...) Potential
	Glycosylation	352	1	N-linked (GlcNAc...) Potential
	Glycosylation	376	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 40		By similarity
	Disulfide bond	71 ↔ 414		By similarity
	Disulfide bond	215 ↔ 465		By similarity
	Disulfide bond	264 ↔ 282		By similarity
	Disulfide bond	436 ↔ 444		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O00085 [UniParc]. Last modified July 1, 1997. Version 1. Checksum: 21CDCB559C96AE66	FASTA	466	51,093
	10 20 30 40 50 60 MGFLAIVLSV ALLFRSTSGT PLGPRGKHSD CNSVDHGYQC FPELSHKWGL YAPYFSLQDE 70 80 90 100 110 120 SPFPLDVPED CHITFVQVLA RHGARSPTHS KTKAYAATIA AIQKSATAFP GKYAFLQSYN 130 140 150 160 170 180 YSLDSEELTP FGRNQLRDLG AQFYERYNAL TRHINPFVRA TDASRVHESA EKFVEGFQTA 190 200 210 220 230 240 RQDDHHANPH QPSPRVDVAI PEGSAYNNTL EHSLCTAFES STVGDDAVAN FTAVFAPAIA 250 260 270 280 290 300 QRLEADLPGV QLSTDDVVNL MAMCPFETVS LTDDAHTLSP FCDLFTATEW TQYNYLLSLD 310 320 330 340 350 360 KYYGYGGGNP LGPVQGVGWA NELMARLTRA PVHDHTCVNN TLDASPATFP LNATLYADFS 370 380 390 400 410 420 HDSNLVSIFW ALGLYNGTAP LSQTSVESVS QTDGYAAAWT VPFAARAYVE MMQCRAEKEP 430 440 450 460 LVRVLVNDRV MPLHGCPTDK LGRCKRDAFV AGLSFAQAGG NWADCF

References

[1]	"The phytase subfamily of histidine acid phosphatases: isolation of genes for two novel phytases from the fungi Aspergillus terreus and Myceliophthora thermophila." Mitchell D.B., Vogel K., Weimann B.J., Pasamontes L., van Loon A.P.G.M. Microbiology 143:245-252(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: 9A1.
[2]	"Biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance." Wyss M., Pasamontes L., Friedlein A., Remy R., Tessier M., Kronenberger A., Middendorf A., Lehmann M., Schnoebelen L., Roethlisberger U., Kusznir E., Wahl G., Mueller F., Lahm H.-W., Vogel K., van Loon A.P.G.M. Appl. Environ. Microbiol. 65:359-366(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-38 AND 192-200, SUBUNIT. Strain: 9A1.
[3]	"Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties." Wyss M., Brugger R., Kronenberger A., Remy R., Fimbel R., Oesterhelt G., Lehmann M., van Loon A.P.G.M. Appl. Environ. Microbiol. 65:367-373(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59805 Genomic DNA. Translation: AAB52507.1.
3D structure databases
ProteinModelPortal	O00085.
SMR	O00085. Positions 31-466.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.8. 536.
Family and domain databases
InterPro	IPR000560. His_Pase_superF_clade-2. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. His_Phos_2. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHYA1_ASPTE

Accession

Primary (citable) accession number: O00085

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	July 1, 1997
Last modified:	April 3, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program