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Protein

Urease

Gene

ure1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.

Cofactori

Ni cationBy similarityNote: Binds 2 nickel ions per subunit.By similarity

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).
Proteins known to be involved in this subpathway in this organism are:
  1. Urease (ure1)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi404Nickel 1; via tele nitrogenBy similarity1
Metal bindingi406Nickel 1; via tele nitrogenBy similarity1
Metal bindingi487Nickel 1; via carbamate groupBy similarity1
Metal bindingi487Nickel 2; via carbamate groupBy similarity1
Binding sitei489SubstrateBy similarity1
Metal bindingi516Nickel 2; via pros nitrogenBy similarity1
Metal bindingi542Nickel 2; via tele nitrogenBy similarity1
Active sitei590Proton donorBy similarity1
Metal bindingi630Nickel 1By similarity1

GO - Molecular functioni

  • nickel cation binding Source: InterPro
  • urease activity Source: PomBase

GO - Biological processi

  • urea catabolic process Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease (EC:3.5.1.5)
Alternative name(s):
Urea amidohydrolase
Gene namesi
Name:ure1
ORF Names:SPAC1952.11c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1952.11c.
PomBaseiSPAC1952.11c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000675291 – 835UreaseAdd BLAST835

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei487N6-carboxylysineBy similarity1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.By similarity

Proteomic databases

MaxQBiO00084.
PRIDEiO00084.

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

BioGridi279019. 5 interactors.
MINTiMINT-4666380.

Structurei

3D structure databases

ProteinModelPortaliO00084.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini399 – 835UreaseAdd BLAST437

Sequence similaritiesi

Belongs to the urease family.Curated
Contains 1 urease domain.Curated

Phylogenomic databases

HOGENOMiHOG000075064.
InParanoidiO00084.
KOiK01427.
OMAiFDSHIHF.
OrthoDBiEOG092C0Z46.
PhylomeDBiO00084.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
cd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
2.30.40.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR008221. Urease.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR002019. Urease_beta.
IPR002026. Urease_gamma/gamma-beta_su.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
PF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001222. Urease. 1 hit.
PRINTSiPR01752. UREASE.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
TIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O00084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPRELHKLT LHQLGSLAQK RLCRGVKLNK LEATSLIASQ IQEYVRDGNH
60 70 80 90 100
SVADLMSLGK DMLGKRHVQP NVVHLLHEIM IEATFPDGTY LITIHDPICT
110 120 130 140 150
TDGNLEHALY GSFLPTPSQE LFPLEEEKLY APENSPGFVE VLEGEIELLP
160 170 180 190 200
NLPRTPIEVR NMGDRPIQVG SHYHFIETNE KLCFDRSKAY GKRLDIPSGT
210 220 230 240 250
AIRFEPGVMK IVNLIPIGGA KLIQGGNSLS KGVFDDSRTR EIVDNLMKQG
260 270 280 290 300
FMHQPESPLN MPLQSARPFV VPRKLYAVMY GPTTNDKIRL GDTNLIVRVE
310 320 330 340 350
KDFTEYGNES VFGGGKVIRD GTGQSSSKSM DECLDTVITN AVIIDHTGIY
360 370 380 390 400
KADIGIKNGY IVGIGKAGNP DTMDNIGENM VIGSSTDVIS AENKIVTYGG
410 420 430 440 450
MDSHVHFICP QQIEEALASG ITTMYGGGTG PSTGTNATTC TPNKDLIRSM
460 470 480 490 500
LRSTDSYPMN IGLTGKGNDS GSSSLKEQIE AGCSGLKLHE DWGSTPAAID
510 520 530 540 550
SCLSVCDEYD VQCLIHTDTL NESSFVEGTF KAFKNRTIHT YHVEGAGGGH
560 570 580 590 600
APDIISLVQN PNILPSSTNP TRPFTTNTLD EELDMLMVCH HLSRNVPEDV
610 620 630 640 650
AFAESRIRAE TIAAEDILQD LGAISMISSD SQAMGRCGEV ISRTWKTAHK
660 670 680 690 700
NKLQRGALPE DEGSGVDNFR VKRYVSKYTI NPAITHGISH IVGSVEIGKF
710 720 730 740 750
ADLVLWDFAD FGARPSMVLK GGMIALASMG DPNGSIPTVS PLMSWQMFGA
760 770 780 790 800
HDPERSIAFV SKASITSGVI ESYGLHKRVE AVKSTRNIGK KDMVYNSYMP
810 820 830
KMTVDPEAYT VTADGKVMEC EPVDKLPLSQ SYFIF
Length:835
Mass (Da):91,182
Last modified:July 1, 1997 - v1
Checksum:iAFB447F39DC4EF4C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002590 Genomic DNA. Translation: BAA19550.1.
CU329670 Genomic DNA. Translation: CAB52575.1.
PIRiT37939.
RefSeqiNP_594813.1. NM_001020242.2.

Genome annotation databases

EnsemblFungiiSPAC1952.11c.1; SPAC1952.11c.1:pep; SPAC1952.11c.
GeneIDi2542563.
KEGGispo:SPAC1952.11c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB002590 Genomic DNA. Translation: BAA19550.1.
CU329670 Genomic DNA. Translation: CAB52575.1.
PIRiT37939.
RefSeqiNP_594813.1. NM_001020242.2.

3D structure databases

ProteinModelPortaliO00084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279019. 5 interactors.
MINTiMINT-4666380.

Protein family/group databases

MEROPSiM38.982.

Proteomic databases

MaxQBiO00084.
PRIDEiO00084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1952.11c.1; SPAC1952.11c.1:pep; SPAC1952.11c.
GeneIDi2542563.
KEGGispo:SPAC1952.11c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1952.11c.
PomBaseiSPAC1952.11c.

Phylogenomic databases

HOGENOMiHOG000075064.
InParanoidiO00084.
KOiK01427.
OMAiFDSHIHF.
OrthoDBiEOG092C0Z46.
PhylomeDBiO00084.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Miscellaneous databases

PROiO00084.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
cd00407. Urease_beta. 1 hit.
cd00390. Urease_gamma. 1 hit.
Gene3Di2.10.150.10. 1 hit.
2.30.40.10. 1 hit.
3.30.280.10. 1 hit.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR008221. Urease.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR002019. Urease_beta.
IPR002026. Urease_gamma/gamma-beta_su.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
PF00699. Urease_beta. 1 hit.
PF00547. Urease_gamma. 1 hit.
[Graphical view]
PIRSFiPIRSF001222. Urease. 1 hit.
PRINTSiPR01752. UREASE.
ProDomiPD002319. Urease_gamma_reg. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF51278. SSF51278. 1 hit.
SSF51338. SSF51338. 1 hit.
SSF51556. SSF51556. 2 hits.
SSF54111. SSF54111. 1 hit.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
TIGR00192. urease_beta. 1 hit.
TIGR00193. urease_gam. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUREA_SCHPO
AccessioniPrimary (citable) accession number: O00084
Secondary accession number(s): Q9URH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 1, 1997
Last modified: October 5, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.