Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dehydrogenase OXI1

Gene

OXI1

Organism
Cochliobolus heterostrophus (strain C4 / ATCC 48331 / race T) (Southern corn leaf blight fungus) (Bipolaris maydis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dehydrogenase; part of the Tox1A locus, one of the 2 loci that mediate the biosynthesis of T-toxin, a family of linear polyketides 37 to 45 carbons in length, of which the major component is 41 carbons, and which leads to high virulence to maize (PubMed:8953776, PubMed:20192833). One of the PKSs (PKS1 or PKS2) could synthesize a precursor, used subsequently by the other PKS as starter unit, to add additional carbons (PubMed:16529376). Variability in the length of the final carbon backbone C35-47 could be achieved by varying the number of condensation cycles, or use of different starter or extender units or might be due to decarboxylation of the penultimate product, catalyzed by DEC1 (PubMed:12236595). Additional proteins are required for the biosynthesis of T-toxin, including oxidoreductases RED1, RED2, RED3, LAM1 and OXI1, as well as esterase TOX9 (PubMed:20192833).4 Publications

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.PROSITE-ProRule annotation

Pathwayi: Mycotoxin biosynthesis

This protein is involved in Mycotoxin biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in Mycotoxin biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42NADPBy similarity1
Active sitei133Proton acceptorPROSITE-ProRule annotation1
Binding sitei137NADPBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrogenase OXI1Curated (EC:1.1.1.1PROSITE-ProRule annotation)
Alternative name(s):
T-toxin biosynthesis protein OXI1Curated
Gene namesi
Name:OXI11 Publication
ORF Names:COCC4DRAFT_155491
OrganismiCochliobolus heterostrophus (strain C4 / ATCC 48331 / race T) (Southern corn leaf blight fungus) (Bipolaris maydis)
Taxonomic identifieri665024 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeBipolaris
Proteomesi
  • UP000012338 Componenti: Unassembled WGS sequence

Pathology & Biotechi

Disruption phenotypei

Significantly reduces the production of T-toxin and decreases the virulence to maize (PubMed:20192833).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_000043764521 – 232Dehydrogenase OXI1Sequence analysisAdd BLAST212

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi117N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliN4WE73.
SMRiN4WE73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

OrthoDBiEOG092C3TSH.

Family and domain databases

InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

N4WE73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTETFKVAIT FVSPSSEALA QSIIDSINKS ADTTRAIKIQ ADMRDTDSPL
60 70 80 90 100
RIIDATICAF GPNIDILVNN AGVESLVSLS ELGLQDFNEC IDVNFRAVVF
110 120 130 140 150
MTKSVIPYLR SPGRIINISS SSAHAGGLSS GIYAASKAAV EALARFWATS
160 170 180 190 200
LGPQGHSVNT VVPGLTQTDM YERIIAEESS AAYHRTVASM TPMGGRVGTP
210 220 230
EDIARIVSLL VEPRSQWVTG QTISATGGLI LL
Length:232
Mass (Da):24,632
Last modified:June 26, 2013 - v1
Checksum:iC603FD41AA28D014
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ943499 Genomic DNA. Translation: ADB23430.1.
KB733545 Genomic DNA. Translation: ENH98548.1.
RefSeqiXP_014072458.1. XM_014216983.1.

Genome annotation databases

EnsemblFungiiENH98548; ENH98548; COCC4DRAFT_155491.
GeneIDi25839391.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiOXI1_COCH4
AccessioniPrimary (citable) accession number: N4WE73
Secondary accession number(s): D2SZX7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2016
Last sequence update: June 26, 2013
Last modified: May 10, 2017
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families