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Protein

Dehydrogenase RED2

Gene

RED2

Organism
Cochliobolus heterostrophus (strain C4 / ATCC 48331 / race T) (Southern corn leaf blight fungus) (Bipolaris maydis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Dehydrogenase; part of the Tox1B locus, one of the 2 loci that mediate the biosynthesis of T-toxin, a family of linear polyketides 37 to 45 carbons in length, of which the major component is 41 carbons, and which leads to high virulence to maize (PubMed:8953776, PubMed:20192833). One of the PKSs (PKS1 or PKS2) could synthesize a precursor, used subsequently by the other PKS as starter unit, to add additional carbons (PubMed:16529376). Variability in the length of the final carbon backbone C35-47 could be achieved by varying the number of condensation cycles, or use of different starter or extender units or might be due to decarboxylation of the penultimate product, catalyzed by DEC1 (PubMed:12236595). Additional proteins are required for the biosynthesis of T-toxin, including oxidoreductases RED1, RED2, RED3, LAM1 and OXI1, as well as esterase TOX9 (PubMed:20192833).4 Publications

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.PROSITE-ProRule annotation

Pathwayi: Mycotoxin biosynthesis

This protein is involved in Mycotoxin biosynthesis.1 Publication
View all proteins of this organism that are known to be involved in Mycotoxin biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei228Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Names & Taxonomyi

Protein namesi
Recommended name:
Dehydrogenase RED21 Publication (EC:1.1.1.1PROSITE-ProRule annotation)
Alternative name(s):
T-toxin biosynthesis protein RED2Curated
Gene namesi
Name:RED21 Publication
ORF Names:COCC4DRAFT_155544
OrganismiCochliobolus heterostrophus (strain C4 / ATCC 48331 / race T) (Southern corn leaf blight fungus) (Bipolaris maydis)
Taxonomic identifieri665024 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaDothideomycetesPleosporomycetidaePleosporalesPleosporineaePleosporaceaeBipolaris
Proteomesi
  • UP000012338 Componenti: Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
Transmembranei47 – 67HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Significantly reduces the production of T-toxin and decreases the virulence to maize (PubMed:20192833).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004376431 – 265Dehydrogenase RED2Add BLAST265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi45N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi74N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi127N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi176N-linked (GlcNAc...)PROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Structurei

3D structure databases

ProteinModelPortaliN4WE43.
SMRiN4WE43.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiEOG092C3QFN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PfamiView protein in Pfam
PF00106. adh_short. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.

Sequencei

Sequence statusi: Complete.

N4WE43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKIESFLLS KLFLCIALCT AYVAFSSAMM EPGLWLLSRP IVPLNRTSTV
60 70 80 90 100
FGLTIVAIGL SALSSWLSKL TLNNFTSDDS WDWSQEIVVV TGASSGIGAE
110 120 130 140 150
IVRLLAELSI KTFILDPVPP DNSVLKNGSV HYYKVDITKP KEVSAAAREI
160 170 180 190 200
QVKFSSPTVL INNAGVGLAK NLLDENETER RHLMNVNLLS QFTTVQEFLP
210 220 230 240 250
AMIEKNHGHI VTMASSASYI SSPQIVSYAA SKAALVGFHE GLGIELVKRY
260
NAKKIRTTLV FAPLL
Length:265
Mass (Da):29,001
Last modified:June 26, 2013 - v1
Checksum:i4598C1D75294BDD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF525909 Genomic DNA. Translation: ACP34152.1.
KB733569 Genomic DNA. Translation: ENH98523.1.
RefSeqiXP_014072433.1. XM_014216958.1.

Genome annotation databases

EnsemblFungiiENH98523; ENH98523; COCC4DRAFT_155544.
GeneIDi25839400.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF525909 Genomic DNA. Translation: ACP34152.1.
KB733569 Genomic DNA. Translation: ENH98523.1.
RefSeqiXP_014072433.1. XM_014216958.1.

3D structure databases

ProteinModelPortaliN4WE43.
SMRiN4WE43.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiENH98523; ENH98523; COCC4DRAFT_155544.
GeneIDi25839400.

Phylogenomic databases

OrthoDBiEOG092C3QFN.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiView protein in InterPro
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
PfamiView protein in Pfam
PF00106. adh_short. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00061. ADH_SHORT. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRED2_COCH4
AccessioniPrimary (citable) accession number: N4WE43
Secondary accession number(s): C3JXE8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2016
Last sequence update: June 26, 2013
Last modified: March 15, 2017
This is version 19 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.