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Protein

Dihydroxy-acid dehydratase

Gene

ilvD

Organism
Mannheimia haemolytica M42548
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O.UniRule annotationSAAS annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvI), Acetolactate synthase (ilvG)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase (ilvI), Acetolactate synthase (ilvG)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi122Iron-sulfur (4Fe-4S)UniRule annotation1
Metal bindingi195Iron-sulfur (4Fe-4S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyaseUniRule annotationSAAS annotationImported
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesisUniRule annotationSAAS annotation
Ligand4Fe-4SUniRule annotationSAAS annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00047; UER00057.
UPA00049; UER00061.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydroxy-acid dehydrataseUniRule annotationSAAS annotation (EC:4.2.1.9UniRule annotationSAAS annotation)
Short name:
DADUniRule annotation
Gene namesi
Name:ilvDUniRule annotationImported
ORF Names:MHH_c13530Imported
OrganismiMannheimia haemolytica M42548Imported
Taxonomic identifieri1316932 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeMannheimia
Proteomesi
  • UP000012987 Componenti: Chromosome

Family & Domainsi

Sequence similaritiesi

Belongs to the IlvD/Edd family.UniRule annotationSAAS annotation

Phylogenomic databases

KOiK01687.
OrthoDBiPOG091H010A.

Family and domain databases

HAMAPiMF_00012. IlvD. 1 hit.
InterProiView protein in InterPro
IPR004404. DihydroxyA_deHydtase.
IPR000581. DiOHA_6PGluconate_deHydtase.
IPR020558. DiOHA_6PGluconate_deHydtase_CS.
IPR037237. IlvD/EDD_N.
PfamiView protein in Pfam
PF00920. ILVD_EDD. 1 hit.
SUPFAMiSSF143975. SSF143975. 1 hit.
TIGRFAMsiTIGR00110. ilvD. 1 hit.
PROSITEiView protein in PROSITE
PS00886. ILVD_EDD_1. 1 hit.
PS00887. ILVD_EDD_2. 1 hit.

Sequencei

Sequence statusi: Complete.

M9X4Q5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPILRSATST QGRNMAGARA LWRATGMKEN DFGKPIIAVV NSFTQFVPGH
60 70 80 90 100
VHLKDMGQLV AAEIEKAGGV AKEFNTIAVD DGIAMGHGGM LYSLPSRDLI
110 120 130 140 150
ADSVEYMVNA HCADAMVCIS NCDKITPGML MAAMRLNIPT IFVSGGPMEA
160 170 180 190 200
GKTKLSDQLI RLDLVDAMIE SADPNVSDER IDAIERSACP TCGSCSGMFT
210 220 230 240 250
ANSMNCLTEA LGLSLPGNGS MLATHADRKE LFLRAGREIV ELCRKYYQQD
260 270 280 290 300
DESVLPRSIA TFDAFENAMS LDIAMGGSSN TVLHLLATAQ EAGVDFKMED
310 320 330 340 350
IDRLSRVVPC LSKIAPNTNK YHMEDVHRAG GIMGLLGELD RAGLIHRHVK
360 370 380 390 400
TVLGLTIEEQ LNQYDIIRNK DEDLHKFFRA GPAGIRTTKA FSQDCRWDTV
410 420 430 440 450
DDDRVNGCIR NVENAVTKEG GLAVLFGNIA EDGCIVKTAG VDESIWKFTG
460 470 480 490 500
TAIVFESQED AVAGILGGKV KEGHIVIIRY EGPKGGPGMQ EMLYPTSYLK
510 520 530 540 550
SMGLGKKCAL LTDGRFSGGT SGLSIGHASP EAASGGAIAL INDGDTVAID
560 570 580 590 600
IPNRSINLMV SDEELAARRA EMEARGDKAW QPLKREREVS FALKVFGHFA
610
TSADKGAVRD KSLLK
Length:615
Mass (Da):66,210
Last modified:June 26, 2013 - v1
Checksum:iEC7100A1602CA11C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP005383 Genomic DNA. Translation: AGK01804.1.
RefSeqiWP_006253708.1. NC_021082.1.

Genome annotation databases

EnsemblBacteriaiAGK01804; AGK01804; MHH_c13530.
GeneIDi15339085.
KEGGimhx:MHH_c13530.
PATRICifig|1316932.3.peg.1313.

Similar proteinsi

Entry informationi

Entry nameiM9X4Q5_MANHA
AccessioniPrimary (citable) accession number: M9X4Q5
Entry historyiIntegrated into UniProtKB/TrEMBL: June 26, 2013
Last sequence update: June 26, 2013
Last modified: November 22, 2017
This is version 33 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported