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Protein

Thymidylate synthase

Gene

thyA2

Organism
Bacillus subtilis XF-1
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotationSAAS annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotationSAAS annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei24dUMPUniRule annotation1
Binding sitei545,10-methylenetetrahydrofolateUniRule annotation1
Active sitei149PROSITE-ProRule annotation1
Active sitei149NucleophileUniRule annotation1
Binding sitei1725,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei180dUMPUniRule annotation1
Binding sitei2665,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi129 – 130dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi169 – 172dUMPUniRule annotation4
Nucleotide bindingi210 – 212dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferaseUniRule annotationSAAS annotation, Transferase
Biological processNucleotide biosynthesisUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotationSAAS annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyA2Imported
Synonyms:thyAUniRule annotation
ORF Names:C663_2054Imported
OrganismiBacillus subtilis XF-1Imported
Taxonomic identifieri1233100 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000011821 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 267Thymidylat_syntInterPro annotationAdd BLAST263

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

KOiK00560.

Family and domain databases

CDDicd00351. TS_Pyrimidine_HMase. 1 hit.
Gene3Di3.30.572.10. 1 hit.
HAMAPiMF_00008. Thymidy_synth_bact. 1 hit.
InterProiView protein in InterPro
IPR023451. Thymidate_synth/dCMP_Mease.
IPR036926. Thymidate_synth/dCMP_Mease_sf.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
PfamiView protein in Pfam
PF00303. Thymidylat_synt. 1 hit.
PRINTSiPR00108. THYMDSNTHASE.
SUPFAMiSSF55831. SSF55831. 1 hit.
TIGRFAMsiTIGR03284. thym_sym. 2 hits.
PROSITEiView protein in PROSITE
PS00091. THYMIDYLATE_SYNTHASE. 1 hit.

Sequencei

Sequence statusi: Complete.

M4KU27-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKMKQYKDL CRHVLEHGEK KGDRTGTGTI STFGYQMRFN LREGFPMLTT
60 70 80 90 100
KKLHFKSIAH ELLWFLKGDT NVRYLQENGV RIWNEWADEN GELGPVYGSQ
110 120 130 140 150
WRSWRGADGE TIDQISRLIE DIKTNPNSRR LIVSAWNVGE IDKMALPPCH
160 170 180 190 200
CLFQFYVSDG KLSCQLYQRS ADVFLGVPFN IASYALLTKM IAHVTGLEPG
210 220 230 240 250
EFIHTFGDVH IYQNHIEQVN LQLERDVRPL PKLRFARKVD SIFNFAFEDF
260
IIKDYDPHPH IKGAVSV
Length:267
Mass (Da):30,905
Last modified:May 29, 2013 - v1
Checksum:iEDB71E42F8876BC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP004019 Genomic DNA. Translation: AGE63830.1.

Genome annotation databases

EnsemblBacteriaiAGE63830; AGE63830; C663_2054.
KEGGibsx:C663_2054.
PATRICifig|1233100.3.peg.2185.

Similar proteinsi

Entry informationi

Entry nameiM4KU27_BACIU
AccessioniPrimary (citable) accession number: M4KU27
Entry historyiIntegrated into UniProtKB/TrEMBL: May 29, 2013
Last sequence update: May 29, 2013
Last modified: February 28, 2018
This is version 36 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported