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Protein

AP-2 complex subunit alpha

Gene

AP2A1

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosisUniRule annotation, Protein transportUniRule annotationSAAS annotation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit alphaUniRule annotation
Gene namesi
Name:AP2A1Imported
OrganismiFelis catus (Cat) (Felis silvestris catus)Imported
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
Proteomesi
  • UP000011712 Componenti: Chromosome E2

Subcellular locationi

Keywords - Cellular componenti

Coated pitUniRule annotation, Membrane

Expressioni

Gene expression databases

BgeeiENSFCAG00000004429.

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000004090.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini739 – 855Alpha_adaptinC2InterPro annotationAdd BLAST117

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili16 – 36Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the adaptor complexes large subunit family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiKOG1077. Eukaryota.
ENOG410XNQE. LUCA.
GeneTreeiENSGT00550000074757.
InParanoidiM3W262.
OMAiHNELFNC.
OrthoDBiEOG091G01JZ.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
3.30.310.10. 1 hit.
InterProiView protein in InterPro
IPR017104. AP2_complex_asu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR003164. Clathrin_a-adaptin_app_sub_C.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013041. Clathrin_app_Ig-like_sf.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR012295. TBP_dom_sf.
PfamiView protein in Pfam
PF01602. Adaptin_N. 1 hit.
PF02296. Alpha_adaptin_C. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PIRSFiPIRSF037091. AP2_complex_alpha. 1 hit.
SMARTiView protein in SMART
SM00809. Alpha_adaptinC2. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.

Sequencei

Sequence statusi: Complete.

M3W262-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSGSSGTG GRGEGRKSKE AEIKRINKEL ANIRSKFKGD KALDGYSKKK
60 70 80 90 100
YVCKLLFIFL LGHDIDFGHM EAVNLLSSNK YTEKQIGYLF ISVLVNSNSE
110 120 130 140 150
LIRLINNAIK NDLASRNPTF MCLALHCIAN VGSREMGEAF AADIPRILVA
160 170 180 190 200
GDSMDSVKQS AALCLLRLYK ASPDLVPMGE WTARVVHLLN DQHMGVVTAA
210 220 230 240 250
VSLITCLCKK NPDDFKTCIS LAVSRLSRIV SSASTDLQDY TYYFVPAPWL
260 270 280 290 300
SVKLLRLLQC YPPPEDAAVK GRLVECLETV LNKAQEPPKS KKVQHSNAKN
310 320 330 340 350
AILFETISLI IHYDSEPNLL VRACNQLGQF LQHRETNLRY LALESMCTLA
360 370 380 390 400
SSEFSHEAVK THIDTVINAL KTERDVSVRQ RAADLLYAMC DRSNAKQIVS
410 420 430 440 450
EMLRYLETAD YAIREEIVLK VAILAEKYAV DYSWYVDTIL NLIRIAGDYV
460 470 480 490 500
SEEVWYRVLQ IVTNRDDVQG YAAKTVFEAL QAPACHENMV KVGGYILGEF
510 520 530 540 550
GNLIAGDPRS SPPVQFSLLH SKFHLCSVAT RALLLSTYIK FINLFPETKA
560 570 580 590 600
TIQGVLRAGS QLRNADVELQ QRAVEYLTLS SVASTDVLAT VLEEMPPFPE
610 620 630 640 650
RESSILAKLK RKKGPGAGSA LDDGRRDPSS NDVNGGVEPT PSTVSTPSPS
660 670 680 690 700
ADLLGLRAAP PPAAPPATSG AGNLLVDVFS DGPAAQPSLG PTPEEAFLSE
710 720 730 740 750
LEPPAPESPM ALLADPAPAA DPGPEDIGPP IPEADELLNK FVCKNNGVLF
760 770 780 790 800
ENQLLQIGVK SEFRQNLGRM YLFYGNKTSV QFQNFSPTVV HPGDLQTHIL
810 820 830 840 850
PLAVQTKRVA AQVDGGAQVQ QVLNIECLRD FLTPPLLSVR FRYGGAPQSL
860 870 880 890 900
TLKLPVTINK FFQPTEMAAQ DFFQRWKQLS LPQQEAQKIF KANHPMDAEV
910 920 930 940 950
TKAKLLGFGS ALLDNVDPNP ENFVGAGIIQ TKALQVGCLL RLEPNAQAQM
960 970
YRLTLRTSKE PVSRHLCELL AQQF
Length:974
Mass (Da):107,064
Last modified:February 28, 2018 - v2
Checksum:i5891647EF05F129A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AANG03035630 Genomic DNA. No translation available.
AANG03035631 Genomic DNA. No translation available.
AANG03035632 Genomic DNA. No translation available.

Genome annotation databases

EnsembliENSFCAT00000004432; ENSFCAP00000004090; ENSFCAG00000004429.

Similar proteinsi

Entry informationi

Entry nameiM3W262_FELCA
AccessioniPrimary (citable) accession number: M3W262
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 2013
Last sequence update: February 28, 2018
Last modified: March 28, 2018
This is version 35 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported