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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Helicobacter pylori HP260BFii
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotationSAAS annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotationSAAS annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. no protein annotated in this organism
  4. Branched-chain-amino-acid aminotransferase (HMPREF1451_00093)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotationSAAS annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. no protein annotated in this organism
  4. Branched-chain-amino-acid aminotransferase (HMPREF1451_00093)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54NADPUniRule annotation1
Binding sitei56NADPUniRule annotation1
Active sitei111UniRule annotation1
Binding sitei137NADP; via amide nitrogenUniRule annotation1
Metal bindingi195Magnesium 1UniRule annotation1
Metal bindingi195Magnesium 2UniRule annotation1
Metal bindingi199Magnesium 1UniRule annotation1
Metal bindingi231Magnesium 2UniRule annotation1
Metal bindingi235Magnesium 2UniRule annotation1
Binding sitei256SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 31NADPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomeraseImported, OxidoreductaseUniRule annotationSAAS annotation
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesisUniRule annotationSAAS annotation
LigandMagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, NADPUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00047; UER00056.
UPA00049; UER00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
ORF Names:HMPREF1451_01058Imported
OrganismiHelicobacter pylori HP260BFiiImported
Taxonomic identifieri1159079 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesHelicobacteraceaeHelicobacter
Proteomesi
  • UP000011953 Componenti: Unassembled WGS sequence

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini18 – 182IlvNInterPro annotationAdd BLAST165
Domaini188 – 327IlvCInterPro annotationAdd BLAST140

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotationSAAS annotation

Phylogenomic databases

OrthoDBiPOG091H063Y.

Family and domain databases

HAMAPiMF_00435. IlvC. 1 hit.
InterProiView protein in InterPro
IPR008927. 6-PGluconate_DH-like_C_sf.
IPR000506. AcH_isomrdctse_C.
IPR013116. IlvN.
IPR013023. KARI.
IPR014359. KARI_prok.
IPR036291. NAD(P)-bd_dom_sf.
PANTHERiPTHR21371. PTHR21371. 1 hit.
PfamiView protein in Pfam
PF01450. IlvC. 1 hit.
PF07991. IlvN. 1 hit.
PIRSFiPIRSF000116. IlvC_gammaproteo. 2 hits.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00465. ilvC. 1 hit.

Sequencei

Sequence statusi: Complete.

M3RN94-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MILALPIYYD KDIDLGVIQS LQVGIIGYGA QGEAQALNLR DSKVKARIGL
60 70 80 90 100
YQGSLSVSKA KAEGFEVLGV KELVQQSDVI MALLPDELHK EVLEKEVIPF
110 120 130 140 150
LKEGQIVGFA HGFSVRFNQV VFPKGVGVIL VAPKGPGSAL REEYLKNRGL
160 170 180 190 200
YHLIAIEQES SKNNAKAVAL SYAKAMGGGR MGVLETNFKE ECESDLFGEQ
210 220 230 240 250
AVLCGGLEAI VRMGFETLIK AGYPEELAYF ECVHEVKLVA DLLHYKGVEG
260 270 280 290 300
LRKHISNTAE FGAIKAREPM GNLLEKRMQK ILKKIQNGAF AKDFLLEKSL
310 320 330
SYPRLNTERK ALKETKIEQI GEILRAPFNH KK
Length:332
Mass (Da):36,703
Last modified:May 1, 2013 - v1
Checksum:i3F0F72F15AEAA77F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
APFB01000042 Genomic DNA. Translation: EMH67374.1.

Genome annotation databases

EnsemblBacteriaiEMH67374; EMH67374; HMPREF1451_01058.
PATRICifig|1159079.3.peg.1015.

Similar proteinsi

Entry informationi

Entry nameiM3RN94_HELPX
AccessioniPrimary (citable) accession number: M3RN94
Entry historyiIntegrated into UniProtKB/TrEMBL: May 1, 2013
Last sequence update: May 1, 2013
Last modified: March 28, 2018
This is version 39 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation
The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteomeImported