ID   M1PDD6_METMZ            Unreviewed;       526 AA.
AC   M1PDD6;
DT   01-MAY-2013, integrated into UniProtKB/TrEMBL.
DT   01-MAY-2013, sequence version 1.
DT   24-JAN-2024, entry version 31.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000256|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000256|HAMAP-Rule:MF_01904};
GN   ORFNames=MmTuc01_3308 {ECO:0000313|EMBL:AGF98562.1};
OS   Methanosarcina mazei Tuc01.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=1236903 {ECO:0000313|EMBL:AGF98562.1, ECO:0000313|Proteomes:UP000011718};
RN   [1] {ECO:0000313|EMBL:AGF98562.1, ECO:0000313|Proteomes:UP000011718}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuc01 {ECO:0000313|EMBL:AGF98562.1,
RC   ECO:0000313|Proteomes:UP000011718};
RX   PubMed=23704185;
RA   Assis das Gracas D., Thiago Juca Ramos R., Vieira Araujo A.C., Zahlouth R.,
RA   Ribeiro Carneiro A., Souza Lopes T., Azevedo Barauna R., Azevedo V.,
RA   Cruz Schneider M.P., Pellizari V.H., Silva A.;
RT   "Complete Genome of a Methanosarcina mazei Strain Isolated from Sediment
RT   Samples from an Amazonian Flooded Area.";
RL   Genome Announc. 1:E00271-13(2013).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000256|HAMAP-
CC       Rule:MF_01904}.
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DR   EMBL; CP004144; AGF98562.1; -; Genomic_DNA.
DR   RefSeq; WP_015413137.1; NC_020389.1.
DR   AlphaFoldDB; M1PDD6; -.
DR   GeneID; 24882834; -.
DR   KEGG; mmaz:MmTuc01_3308; -.
DR   HOGENOM; CLU_517433_0_0_2; -.
DR   Proteomes; UP000011718; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   NCBIfam; TIGR02751; PEPCase_arch; 1.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Pyruvate {ECO:0000313|EMBL:AGF98562.1}.
SQ   SEQUENCE   526 AA;  58122 MW;  4F1FA9A95A18BE45 CRC64;
     MSKKATYPKV MCTQHPDSAS KYISTQEEPG EAIEAAVVFG CDEYMPDYEG KATPYHQNVQ
     IVSRFIEETD LIPGKDIFIT PRAPSAAQEN RFRQLMVMMS IAEANHGALE YSDVQAINEF
     VHPMTGTVGE ILDAQQHMVD VGELAKKEFG VAMEVPRIIP LIEDAPALLH AKELAENTLL
     SWEKRFGAAP EKFRVFLGKS DSALSFGHVA STLSCKYAIS GISELDSELD TSTGIIFGAG
     TLPFRGHLSL KNAENFFREY RGIGTITLQS ALRYSHDKGE AEALVRLAEE RLPESPDIYS
     GEEKEEIVNL IGIFGARYNR IIREMSCTIN QLAGLLPQQR DRLMHKGTGG YSRNVPDISG
     LVCLCRNDVG KELSASMPAE DLNLPRAIKF TGALYSIGLP PEFIGTGTAL EEAREKLGEE
     ACERLLKKYF PSLGSDLSFA SGYLDLNVAS RFLPEACLKE IRKDIEVLRD TFSLKVQPEP
     SYRILLEMMQ PDLLQAGTKG NCMDEEVSQL VCSTLTKMGK IRKALG
//