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M1E1F6 (M1E1F6_9RHIZ) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
EC=5.4.11.99 PDB 4GIN
Gene names
Name:mutB PDB 4GIN
OrganismRhizobium sp. MX-45 PDB 4GIN
Taxonomic identifier1071045 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Protein attributes

Sequence length584 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region88 – 914Glucose binding PDB 4H7V
Region88 – 914Sucrose binding PDB 4H8U
Region281 – 2833Sucrose binding PDB 4H8U
Region352 – 3554Sulfate binding PDB 4H8H
Region353 – 3542Glucose binding PDB 4H7V
Region353 – 3542Sucrose binding PDB 4H8U

Sites

Metal binding491Calcium PDB 4GIN PDB 4H2C PDB 4H7V PDB 4H8H PDB 4H8U PDB 4H8V PDB 4HA1
Metal binding511Calcium PDB 4GIN PDB 4H2C PDB 4H7V PDB 4H8H PDB 4H8U PDB 4H8V PDB 4HA1
Metal binding531Calcium PDB 4GIN PDB 4H2C PDB 4H7V PDB 4H8H PDB 4H8U PDB 4H8V PDB 4HA1
Metal binding551Calcium; via carbonyl oxygen PDB 4GIN PDB 4H2C PDB 4H7V PDB 4H8H PDB 4H8U PDB 4H8V PDB 4HA1
Metal binding571Calcium PDB 4GIN PDB 4H2C PDB 4H7V PDB 4H8H PDB 4H8U PDB 4H8V PDB 4HA1
Binding site1311Glucose PDB 4H7V
Binding site1311Sucrose PDB 4H8U
Binding site1911Glucose PDB 4H7V
Binding site1911Sucrose PDB 4H8U
Binding site2811Glucose PDB 4H7V
Binding site4131Sucrose PDB 4H8U
Binding site4411Glucose PDB 4H7V
Binding site4411Sucrose PDB 4H8U

Sequences

Sequence LengthMass (Da)Tools
M1E1F6 [UniParc].

Last modified April 3, 2013. Version 1.
Checksum: E26FB3B877608B86

FASTA58466,739
        10         20         30         40         50         60 
MLMKRLFAAS LMLAFSSVSS VRAEEAVKPG APWWKSAVFY QVYPRSFKDT NGDGIGDFKG 

        70         80         90        100        110        120 
LTEKLDYLKG LGIDAIWINP HYASPNTDNG YDISDYREVM KEYGTMEDFD RLMAELKKRG 

       130        140        150        160        170        180 
MRLMVDVVIN HSSDQHEWFK SSRASKDNPY RDYYFWRDGK DGHEPNNYPS FFGGSAWEKD 

       190        200        210        220        230        240 
PVTGQYYLHY FGRQQPDLNW DTPKLREELY AMLRFWLDKG VSGMRFDTVA TYSKTPGFPD 

       250        260        270        280        290        300 
LTPEQMKNFA EAYTQGPNLH RYLQEMHEKV FDHYDAVTAG EIFGAPLNQV PLFIDSRRKE 

       310        320        330        340        350        360 
LDMAFTFDLI CYDRALDRWH TIPRTLADFR QTIDKVDAIA GEYGWNTFFL GNHDNPRAVS 

       370        380        390        400        410        420 
HFGDDRPQWR EASAKALATV TLTQRGTPFI FQGDELGMTN YPFKTLQDFD DIEVKGFFQD 

       430        440        450        460        470        480 
YVETGKATAE ELLTNVALTS RDNARTPFQW DDSANAGFTT GKPWLKVNPN YTEINAAREI 

       490        500        510        520        530        540 
GDPKSVYSFY RNLISIRHET PALSTGSYRD IDPSNADVYA YTRSQDGETY LVVVNFKAEP 

       550        560        570        580 
RSFTLPDGMH IAETLIESSS PAAPAAGAAS LELQPWQSGI YKVK 

« Hide

References

[1]"Insights into product binding in sucrose isomerases from crystal structures of MutB from Rhizobium sp."
Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
Submitted (SEP-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
[2]"Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity."
Lipski A., Watzlawick H., Ravaud S., Robert X., Rhimi M., Haser R., Mattes R., Aghajari N.
Acta Crystallogr. D 69:298-307(2013)
Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
+Additional computationally mapped references.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GINX-ray1.90A1-584[»]
4H2CX-ray1.70A28-584[»]
4H7VX-ray1.80A/B28-584[»]
4H8HX-ray2.00A/B28-584[»]
4H8UX-ray2.00A/B28-584[»]
4H8VX-ray1.95A/B28-584[»]
4HA1X-ray2.20A/B28-584[»]
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameM1E1F6_9RHIZ
AccessionPrimary (citable) accession number: M1E1F6
Entry history
Integrated into UniProtKB/TrEMBL: April 3, 2013
Last sequence update: April 3, 2013
Last modified: June 11, 2014
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)