Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

M1E1F6

- M1E1F6_9RHIZ

UniProt

M1E1F6 - M1E1F6_9RHIZ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Submitted name:

Sucrose isomerase

Gene

mutB

Organism
Rhizobium sp. MX-45
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli

Functioni

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi49 – 491CalciumImported
Metal bindingi51 – 511CalciumImported
Metal bindingi53 – 531CalciumImported
Metal bindingi55 – 551Calcium; via carbonyl oxygenImported
Metal bindingi57 – 571CalciumImported
Binding sitei131 – 1311GlucoseImported
Binding sitei191 – 1911GlucoseImported
Binding sitei281 – 2811GlucoseImported
Binding sitei441 – 4411GlucoseImported

GO - Molecular functioni

  1. catalytic activity Source: InterPro
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Ligandi

CalciumImported, Metal-bindingImported

Names & Taxonomyi

Protein namesi
Submitted name:
Sucrose isomeraseImported (EC:5.4.11.99Imported)
Gene namesi
Name:mutBImported
OrganismiRhizobium sp. MX-45Imported
Taxonomic identifieri1071045 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4GINX-ray1.90A1-584[»]
4H2CX-ray1.70A28-584[»]
4H7VX-ray1.80A/B28-584[»]
4H8HX-ray2.00A/B28-584[»]
4H8UX-ray2.00A/B28-584[»]
4H8VX-ray1.95A/B28-584[»]
4HA1X-ray2.20A/B28-584[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni88 – 914Glucose bindingImported
Regioni353 – 3542Glucose bindingImported

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

M1E1F6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLMKRLFAAS LMLAFSSVSS VRAEEAVKPG APWWKSAVFY QVYPRSFKDT
60 70 80 90 100
NGDGIGDFKG LTEKLDYLKG LGIDAIWINP HYASPNTDNG YDISDYREVM
110 120 130 140 150
KEYGTMEDFD RLMAELKKRG MRLMVDVVIN HSSDQHEWFK SSRASKDNPY
160 170 180 190 200
RDYYFWRDGK DGHEPNNYPS FFGGSAWEKD PVTGQYYLHY FGRQQPDLNW
210 220 230 240 250
DTPKLREELY AMLRFWLDKG VSGMRFDTVA TYSKTPGFPD LTPEQMKNFA
260 270 280 290 300
EAYTQGPNLH RYLQEMHEKV FDHYDAVTAG EIFGAPLNQV PLFIDSRRKE
310 320 330 340 350
LDMAFTFDLI CYDRALDRWH TIPRTLADFR QTIDKVDAIA GEYGWNTFFL
360 370 380 390 400
GNHDNPRAVS HFGDDRPQWR EASAKALATV TLTQRGTPFI FQGDELGMTN
410 420 430 440 450
YPFKTLQDFD DIEVKGFFQD YVETGKATAE ELLTNVALTS RDNARTPFQW
460 470 480 490 500
DDSANAGFTT GKPWLKVNPN YTEINAAREI GDPKSVYSFY RNLISIRHET
510 520 530 540 550
PALSTGSYRD IDPSNADVYA YTRSQDGETY LVVVNFKAEP RSFTLPDGMH
560 570 580
IAETLIESSS PAAPAAGAAS LELQPWQSGI YKVK
Length:584
Mass (Da):66,739
Last modified:April 3, 2013 - v1
Checksum:iE26FB3B877608B86
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4GIN X-ray 1.90 A 1-584 [» ]
4H2C X-ray 1.70 A 28-584 [» ]
4H7V X-ray 1.80 A/B 28-584 [» ]
4H8H X-ray 2.00 A/B 28-584 [» ]
4H8U X-ray 2.00 A/B 28-584 [» ]
4H8V X-ray 1.95 A/B 28-584 [» ]
4HA1 X-ray 2.20 A/B 28-584 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view ]
SMARTi SM00642. Aamy. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Insights into product binding in sucrose isomerases from crystal structures of MutB from Rhizobium sp."
    Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
    Submitted (SEP-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
  2. "Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity."
    Lipski A., Watzlawick H., Ravaud S., Robert X., Rhimi M., Haser R., Mattes R., Aghajari N.
    Acta Crystallogr. D 69:298-307(2013)
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.

Entry informationi

Entry nameiM1E1F6_9RHIZ
AccessioniPrimary (citable) accession number: M1E1F6
Entry historyi
Integrated into UniProtKB/TrEMBL: April 3, 2013
Last sequence update: April 3, 2013
Last modified: October 29, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported

External Data

Dasty 3