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M1E1F6

- M1E1F6_9RHIZ

UniProt

M1E1F6 - M1E1F6_9RHIZ

Protein
Submitted name:

Sucrose isomerase

Gene

mutB

Organism
Rhizobium sp. MX-45
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 9 (01 Oct 2014)
      Sequence version 1 (03 Apr 2013)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi49 – 491CalciumImported
    Metal bindingi51 – 511CalciumImported
    Metal bindingi53 – 531CalciumImported
    Metal bindingi55 – 551Calcium; via carbonyl oxygenImported
    Metal bindingi57 – 571CalciumImported
    Binding sitei131 – 1311GlucoseImported
    Binding sitei191 – 1911GlucoseImported
    Binding sitei281 – 2811GlucoseImported
    Binding sitei441 – 4411GlucoseImported

    GO - Molecular functioni

    1. catalytic activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Ligandi

    CalciumImported, Metal-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Sucrose isomeraseImported (EC:5.4.11.99Imported)
    Gene namesi
    Name:mutBImported
    OrganismiRhizobium sp. MX-45Imported
    Taxonomic identifieri1071045 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GINX-ray1.90A1-584[»]
    4H2CX-ray1.70A28-584[»]
    4H7VX-ray1.80A/B28-584[»]
    4H8HX-ray2.00A/B28-584[»]
    4H8UX-ray2.00A/B28-584[»]
    4H8VX-ray1.95A/B28-584[»]
    4HA1X-ray2.20A/B28-584[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni88 – 914Glucose bindingImported
    Regioni353 – 3542Glucose bindingImported

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR022567. DUF3459.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF11941. DUF3459. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    M1E1F6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLMKRLFAAS LMLAFSSVSS VRAEEAVKPG APWWKSAVFY QVYPRSFKDT    50
    NGDGIGDFKG LTEKLDYLKG LGIDAIWINP HYASPNTDNG YDISDYREVM 100
    KEYGTMEDFD RLMAELKKRG MRLMVDVVIN HSSDQHEWFK SSRASKDNPY 150
    RDYYFWRDGK DGHEPNNYPS FFGGSAWEKD PVTGQYYLHY FGRQQPDLNW 200
    DTPKLREELY AMLRFWLDKG VSGMRFDTVA TYSKTPGFPD LTPEQMKNFA 250
    EAYTQGPNLH RYLQEMHEKV FDHYDAVTAG EIFGAPLNQV PLFIDSRRKE 300
    LDMAFTFDLI CYDRALDRWH TIPRTLADFR QTIDKVDAIA GEYGWNTFFL 350
    GNHDNPRAVS HFGDDRPQWR EASAKALATV TLTQRGTPFI FQGDELGMTN 400
    YPFKTLQDFD DIEVKGFFQD YVETGKATAE ELLTNVALTS RDNARTPFQW 450
    DDSANAGFTT GKPWLKVNPN YTEINAAREI GDPKSVYSFY RNLISIRHET 500
    PALSTGSYRD IDPSNADVYA YTRSQDGETY LVVVNFKAEP RSFTLPDGMH 550
    IAETLIESSS PAAPAAGAAS LELQPWQSGI YKVK 584
    Length:584
    Mass (Da):66,739
    Last modified:April 3, 2013 - v1
    Checksum:iE26FB3B877608B86
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GIN X-ray 1.90 A 1-584 [» ]
    4H2C X-ray 1.70 A 28-584 [» ]
    4H7V X-ray 1.80 A/B 28-584 [» ]
    4H8H X-ray 2.00 A/B 28-584 [» ]
    4H8U X-ray 2.00 A/B 28-584 [» ]
    4H8V X-ray 1.95 A/B 28-584 [» ]
    4HA1 X-ray 2.20 A/B 28-584 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR022567. DUF3459.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF11941. DUF3459. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Insights into product binding in sucrose isomerases from crystal structures of MutB from Rhizobium sp."
      Lipski A., Watzlawick H., Ravaud S., Robert X., Haser R., Mattes R., Aghajari N.
      Submitted (SEP-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.
    2. "Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity."
      Lipski A., Watzlawick H., Ravaud S., Robert X., Rhimi M., Haser R., Mattes R., Aghajari N.
      Acta Crystallogr. D 69:298-307(2013)
      Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 28-584 IN COMPLEX WITH CALCIUM.

    Entry informationi

    Entry nameiM1E1F6_9RHIZ
    AccessioniPrimary (citable) accession number: M1E1F6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 3, 2013
    Last sequence update: April 3, 2013
    Last modified: October 1, 2014
    This is version 9 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3