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M1E1F3

- M1E1F3_9RHIZ

UniProt

M1E1F3 - M1E1F3_9RHIZ

Protein
Submitted name:

Sucrose isomerase

Gene

mutB

Organism
Rhizobium sp. MX-45
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 8 (01 Oct 2014)
      Sequence version 1 (03 Apr 2013)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi22 – 221CalciumImported
    Metal bindingi24 – 241CalciumImported
    Metal bindingi26 – 261CalciumImported
    Metal bindingi28 – 281Calcium; via carbonyl oxygenImported
    Metal bindingi30 – 301CalciumImported
    Binding sitei144 – 1441Glucose
    Binding sitei284 – 2841Glucose
    Binding sitei327 – 3271Glucose
    Binding sitei386 – 3861Glucose

    GO - Molecular functioni

    1. catalytic activity Source: InterPro
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Ligandi

    CalciumImported, Metal-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Sucrose isomeraseImported (EC:5.4.11.99Imported)
    Gene namesi
    Name:mutBImported
    OrganismiRhizobium sp. MX-45Imported
    Taxonomic identifieri1071045 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupRhizobium

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4GI6X-ray2.15A/B1-557[»]
    4GI8X-ray1.95A/B1-557[»]
    4GI9X-ray2.15A/B1-557[»]
    4GIAX-ray2.01A/B1-557[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni254 – 2563Glucose binding

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR022567. DUF3459.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF11941. DUF3459. 1 hit.
    [Graphical view]
    SMARTiSM00642. Aamy. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    M1E1F3-1 [UniParc]FASTAAdd to Basket

    « Hide

    KPGAPWWKSA VFYQVYPRSF KDTNGDGIGD FKGLTEKLDY LKGLGIDAIW    50
    INPHYASPNT DNGYDISDYR EVMKEYGTME DFDRLMAELK KRGMRLMVDV 100
    VINHSSDQHE WFKSSRASKD NPYRDYYFWR DGKDGHEPNN YPSFFGGSAW 150
    EKDPVTGQYY LHYLGRQQPD LNWDTPKLRE ELYAMLRFWL DKGVSGMRFD 200
    TVATYSKTPG FPDLTPEQMK NFAEAYTQGP NLHRYLQEMH EKVFDHYDAV 250
    TAGEIFGAPL NQVPLFIDSR RKELDMAFTF DLIRYDRALD RWHTIPRTLA 300
    DFRQTIDKVD AIAGEYGWNT FFLGNHDNPR AVSHFGDDRP QWREASAKAL 350
    ATVTLTQRGT PFIFQGDELG MTNYPFKTLQ DFDDIEVKGF FQDYVETGKA 400
    TAEELLTNVA LTSRDNARTP FQWDDSANAG FTTGKPWLKV NPNYTEINAA 450
    REIGDPKSVY SFYRNLISIR HETPALSTGS YRDIDPSNAD VYAYTRSQDG 500
    ETYLVVVNFK AEPRSFTLPD GMHIAETLIE SSSPAAPAAG AASLELQPWQ 550
    SGIYKVK 557
    Length:557
    Mass (Da):63,830
    Last modified:April 3, 2013 - v1
    Checksum:i536AFD407111E3E4
    GO

    Cross-referencesi

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4GI6 X-ray 2.15 A/B 1-557 [» ]
    4GI8 X-ray 1.95 A/B 1-557 [» ]
    4GI9 X-ray 2.15 A/B 1-557 [» ]
    4GIA X-ray 2.01 A/B 1-557 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR022567. DUF3459.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR006589. Glyco_hydro_13_sub_cat_dom.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF11941. DUF3459. 1 hit.
    [Graphical view ]
    SMARTi SM00642. Aamy. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity."
      Lipski A., Watzlawick H., Ravaud S., Robert X., Rhimi M., Haser R., Mattes R., Aghajari N.
      Acta Crystallogr. D 69:298-307(2013)
      Cited for: X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) IN COMPLEX WITH CALCIUM AND GLUCOSE.

    Entry informationi

    Entry nameiM1E1F3_9RHIZ
    AccessioniPrimary (citable) accession number: M1E1F3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: April 3, 2013
    Last sequence update: April 3, 2013
    Last modified: October 1, 2014
    This is version 8 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3