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Protein

Sterol 24-C-methyltransferase

Gene

ERG6

Organism
Magnaporthe oryzae (strain Y34) (Rice blast fungus) (Pyricularia oryzae)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the methyl transfer from S-adenosyl-methionine to the C-24 of zymosterol to form fecosterol.By similarity

Catalytic activityi

S-adenosyl-L-methionine + 5-alpha-cholesta-8,24-dien-3-beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5-alpha-cholest-8-en-3-beta-ol.

Pathway:iergosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes ergosterol from zymosterol.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Sterol 24-C-methyltransferase (OOU_Y34scaffold00193g1), Sterol 24-C-methyltransferase (OOU_Y34scaffold00066g7), Sterol 24-C-methyltransferase (ERG6)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway ergosterol biosynthesis, which is itself part of Steroid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ergosterol from zymosterol, the pathway ergosterol biosynthesis and in Steroid metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00768; UER00760.

Names & Taxonomyi

Protein namesi
Recommended name:
Sterol 24-C-methyltransferase (EC:2.1.1.41)
Alternative name(s):
Delta(24)-sterol C-methyltransferase
Gene namesi
Name:ERG6
ORF Names:OOU_Y34scaffold00069g5
OrganismiMagnaporthe oryzae (strain Y34) (Rice blast fungus) (Pyricularia oryzae)
Taxonomic identifieri1143189 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeMagnaporthalesMagnaporthaceaeMagnaporthe
ProteomesiUP000011086 Componenti: Unassembled WGS sequence

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 390390Sterol 24-C-methyltransferasePRO_0000423543Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliL7IP31.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Erg6/SMT family.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR025714. Methyltranfer_dom.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

L7IP31-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKQQHPSSK TQITRRRDTA DMTFEQVLHK GSSANMGGLS SMLGKDREAS
60 70 80 90 100
KTAVDQYFRH WDGKTAKTET TKVREERKAD YATLTRQYYN LVTDFYEYGW
110 120 130 140 150
GQSFHFCTFA PGETFASAIT RYEHTLAHRI GIKKGMKVLD IGCGVGGPAR
160 170 180 190 200
QIAKFTGANI TGITINEYQV ERARRYAEME GYGAGEQLKF VQGDFMALPF
210 220 230 240 250
EKETFDAVYS IEATVHAPKL EDVYKQIFNV LKPGGIFGLY EWVMTDAYDE
260 270 280 290 300
NDPHHKEIRF GIEHGGGIAN LQTAQTAIAA IKAAGFELLE SEDLADNSDR
310 320 330 340 350
APWYWPLGGN AWQYASTFGD ILSTFTMTPA GRAIAHTVLG VVESIGLVPP
360 370 380 390
GTKKTADSLS TTAAALVAGG KEGLFTPMFL MVARKPVAKE
Length:390
Mass (Da):43,036
Last modified:March 6, 2013 - v1
Checksum:i4442B9609B8DA9D5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti246 – 2461D → N in AAX07631 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY849610 mRNA. Translation: AAX07631.1.
JH793273 Genomic DNA. Translation: ELQ44666.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY849610 mRNA. Translation: AAX07631.1.
JH793273 Genomic DNA. Translation: ELQ44666.1.

3D structure databases

ProteinModelPortaliL7IP31.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00768; UER00760.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR025714. Methyltranfer_dom.
IPR030384. MeTrfase_SMT.
IPR029063. SAM-dependent_MTases.
IPR013705. Sterol_MeTrfase_C.
[Graphical view]
PfamiPF13847. Methyltransf_31. 1 hit.
PF08498. Sterol_MT_C. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51685. SAM_MT_ERG6_SMT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Dong H.T., Peng Y.L., Chen B.S., Li Y.Z., Li D.B.
    Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Y34.
    Tissue: Conidium.
  2. "Comparative analysis of the genomes of two field isolates of the rice blast fungus Magnaporthe oryzae."
    Xue M., Yang J., Li Z., Hu S., Yao N., Dean R.A., Zhao W., Shen M., Zhang H., Li C., Liu L., Cao L., Xu X., Xing Y., Hsiang T., Zhang Z., Xu J.-R., Peng Y.-L.
    PLoS Genet. 8:E1002869-E1002869(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Y34.

Entry informationi

Entry nameiERG6_MAGOY
AccessioniPrimary (citable) accession number: L7IP31
Secondary accession number(s): A4R5U1, G4NGD4, Q5EN22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 18, 2013
Last sequence update: March 6, 2013
Last modified: July 22, 2015
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.