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Protein

Glutathione synthetase

Gene

MDA_GLEAN10022760

Organism
Myotis davidii (David's myotis)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glutathione synthetase (MDA_GLEAN10022760)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei136SubstrateUniRule annotation1
Metal bindingi155MagnesiumUniRule annotation1
Binding sitei155ATPUniRule annotation1
Metal bindingi157MagnesiumUniRule annotation1
Binding sitei231SubstrateUniRule annotation1
Binding sitei316ATPUniRule annotation1
Metal bindingi379MagnesiumUniRule annotation1
Binding sitei386ATPUniRule annotation1
Binding sitei436ATPUniRule annotation1
Binding sitei461SubstrateUniRule annotation1
Binding sitei463ATPUniRule annotation1
Binding sitei469ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi375 – 384ATPUniRule annotation10
Nucleotide bindingi409 – 412ATPUniRule annotation4

GO - Molecular functioni

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processGlutathione biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
Gene namesi
ORF Names:MDA_GLEAN10022760Imported
OrganismiMyotis davidii (David's myotis)Imported
Taxonomic identifieri225400 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaChiropteraMicrochiropteraVespertilionidaeMyotis
Proteomesi
  • UP000010556 Componenti: Unassembled WGS sequence

Interactioni

GO - Molecular functioni

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini216 – 313GSH_synthaseInterPro annotationAdd BLAST98

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Family and domain databases

CDDicd00228 eu-GS, 1 hit
Gene3Di1.10.1080.10, 2 hits
3.30.1490.50, 1 hit
3.30.1490.80, 3 hits
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit

Sequencei

Sequence statusi: Complete.

L5LUE5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPTRSIYKV GMASSWGSFL QDERQLQELA RQAVDRALAE GVLLRTTQEP
60 70 80 90 100
SSSDVVSYAP FTLFPSLVPS ALLEQAYAVQ MDFNLLVDAV SQNAAFLEQT
110 120 130 140 150
LSSTIKRDTF TARLFDIYKQ VLKEGIAQTV FLGLNRSDYM FQRNADGSPA
160 170 180 190 200
LKQIEINTVS ASFGGLASRT PAVHRHVLNV LGKTKEAARI LSNNPSKGLA
210 220 230 240 250
LGIAKAWELY GSAKALVLLI AQEKERNVFD QRAIENELLA RNIRVIRRRF
260 270 280 290 300
EDVSEKGSLD QDRKLFLDGQ EIAVVYFRDG YMPSQYNQQN WEARLLLERS
310 320 330 340 350
CSVKCPDIAT QLAGTKKVQQ ELSRVGVLEV LLPDQPEAVA RLRATFAGLY
360 370 380 390 400
SLDLGEEGDQ AIAEALAAPN RFVLKPQREG GGNNLYGEEM VQTLERLKDS
410 420 430 440 450
EERASYILME KIEPEPFGNC LLRPGCPVRV VQCISELGIF GVYVRQGKTL
460 470 480
VMNKHVGHLL RTKATEHADG GVVAGVAVLD NPYPV
Length:485
Mass (Da):53,689
Last modified:March 6, 2013 - v1
Checksum:i46883C3CAB765E30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KB107934 Genomic DNA Translation: ELK29645.1

Similar proteinsi

Entry informationi

Entry nameiL5LUE5_MYODS
AccessioniPrimary (citable) accession number: L5LUE5
Entry historyiIntegrated into UniProtKB/TrEMBL: March 6, 2013
Last sequence update: March 6, 2013
Last modified: February 28, 2018
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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