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Protein

Glutathione synthetase

Gene

PAL_GLEAN10024259

Organism
Pteropus alecto (Black flying fox)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glutathione synthetase (PAL_GLEAN10024259)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei195SubstrateUniRule annotation1
Metal bindingi214MagnesiumUniRule annotation1
Binding sitei214ATPUniRule annotation1
Metal bindingi216MagnesiumUniRule annotation1
Binding sitei290SubstrateUniRule annotation1
Binding sitei375ATPUniRule annotation1
Metal bindingi438MagnesiumUniRule annotation1
Binding sitei445ATPUniRule annotation1
Binding sitei495ATPUniRule annotation1
Binding sitei520SubstrateUniRule annotation1
Binding sitei522ATPUniRule annotation1
Binding sitei528ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi434 – 443ATPUniRule annotation10
Nucleotide bindingi468 – 471ATPUniRule annotation4

GO - Molecular functioni

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processGlutathione biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00142; UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
Gene namesi
ORF Names:PAL_GLEAN10024259Imported
OrganismiPteropus alecto (Black flying fox)Imported
Taxonomic identifieri9402 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaChiropteraMegachiropteraPteropodidaePteropodinaePteropus
Proteomesi
  • UP000010552 Componenti: Unassembled WGS sequence

Interactioni

GO - Molecular functioni

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini275 – 372GSH_synthaseInterPro annotationAdd BLAST98

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili66 – 86Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

InParanoidiL5JXR8

Family and domain databases

CDDicd00228 eu-GS, 1 hit
Gene3Di1.10.1080.10, 2 hits
3.30.1490.50, 1 hit
3.30.1490.80, 3 hits
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit

Sequencei

Sequence statusi: Complete.

L5JXR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAVAAAHIS FQNHIALKGP VLPPPPGMLA SLLGKGILLA LSSVLHVPHT
60 70 80 90 100
FCPWQVGMAN SWGNLLQNEQ QLEELAQQAV DRALAEGVLL RTSQEPSSSD
110 120 130 140 150
VDRLNLPILR RVLWCAIPFT TLFPSLVPSA LLEQAYAVQM DFNLLVDAVS
160 170 180 190 200
QNAAFLEQTL SSTIKRDDFT ARLFDIHKQV LKEGIAQTVF LGLNRSDYMF
210 220 230 240 250
QRNADGSPAL KQIEINTISA SFGGLASRTP AVHRHVLSVL SKTKEAAKIL
260 270 280 290 300
SNNPSKGLAL GIAKAWELYG SANALVLLIA QEKERNIFDQ RAIEIELLTR
310 320 330 340 350
NIHVIRRTFE DISEKGSLDQ DRKLFVDGQE IAVVYFRDGY VPSQYSQQNW
360 370 380 390 400
EARLLLERSC AVKCPDIATQ LAGTKKVQQE LSRVGVLEVL LPGQPEAVAR
410 420 430 440 450
LRATFAGLYS LDMGEEGDQA ITEALAAPSR FVLKPQREGG GNNLYGEEMV
460 470 480 490 500
QVLEQLKDSE ERASYILMEK IEPEPFGNCL LRPRSPVQVV QCISELGIFG
510 520 530 540
VYVRQGKTLV MNKHVGHLLR TKAIEHADGG VAAGVAVLDN PYPV
Length:544
Mass (Da):59,776
Last modified:March 6, 2013 - v1
Checksum:i947BCE83C29D490B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KB031072 Genomic DNA Translation: ELK04125.1

Similar proteinsi

Entry informationi

Entry nameiL5JXR8_PTEAL
AccessioniPrimary (citable) accession number: L5JXR8
Entry historyiIntegrated into UniProtKB/TrEMBL: March 6, 2013
Last sequence update: March 6, 2013
Last modified: February 28, 2018
This is version 23 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health