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Protein

LexA repressor

Gene

lexA

Organism
Pseudomonas putida CSV86
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair.UniRule annotation

Catalytic activityi

Hydrolysis of Ala-|-Gly bond in repressor LexA.UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125For autocatalytic cleavage activityUniRule annotation1
Active sitei162For autocatalytic cleavage activityUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi28 – 48H-T-H motifUniRule annotationAdd BLAST21

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, HydrolaseUniRule annotationSAAS annotationImported, RepressorUniRule annotationSAAS annotation
Biological processDNA damage, DNA repairUniRule annotationSAAS annotation, DNA replicationUniRule annotationSAAS annotation, SOS responseUniRule annotationSAAS annotation, Transcription, Transcription regulationUniRule annotationSAAS annotation

Protein family/group databases

MEROPSiS24.001.

Names & Taxonomyi

Protein namesi
Recommended name:
LexA repressorUniRule annotationSAAS annotation (EC:3.4.21.88UniRule annotation)
Gene namesi
Name:lexAUniRule annotation
ORF Names:CSV86_23322Imported
OrganismiPseudomonas putida CSV86Imported
Taxonomic identifieri1005395 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000010448 Componenti: Unassembled WGS sequence

PTM / Processingi

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei90 – 91Cleavage; by autolysisUniRule annotation2

Keywords - PTMi

Autocatalytic cleavageUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 65LexA_DNA_bindInterPro annotationAdd BLAST65
Domaini120 – 187Peptidase_S24InterPro annotationAdd BLAST68

Sequence similaritiesi

Belongs to the peptidase S24 family.UniRule annotationSAAS annotation

Phylogenomic databases

OrthoDBiPOG091H029U.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
HAMAPiMF_00015. LexA. 1 hit.
InterProiView protein in InterPro
IPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR006197. Peptidase_S24_LexA.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
PfamiView protein in Pfam
PF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
PRINTSiPR00726. LEXASERPTASE.
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR00498. lexA. 1 hit.

Sequencei

Sequence statusi: Complete.

L1LUV4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKLTPRQAE ILAFIKRCLE DNGFPPTRAE IAQELGFKSP NAAEEHLKAL
60 70 80 90 100
ARKGAIEMTP GASRGIRIPG FEARPQQDDS SLPIIGRVAA GAPILAEQHI
110 120 130 140 150
EESCAINPSF FHPRADYLLR VHGMSMKDVG IFDGDLLAVH TAREARNGQI
160 170 180 190 200
VVARLGDEVT VKRFKREGSK VWLIAENPEF APIEVNLKEQ DLVIEGLSVG

VIRR
Length:204
Mass (Da):22,465
Last modified:March 6, 2013 - v1
Checksum:iD22A38594D0680FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AMWJ01000154 Genomic DNA. Translation: EKX82778.1.
RefSeqiWP_009404623.1. NZ_AMWJ01000154.1.

Genome annotation databases

EnsemblBacteriaiEKX82778; EKX82778; CSV86_23322.
PATRICifig|1005395.3.peg.4501.

Similar proteinsi

Entry informationi

Entry nameiL1LUV4_PSEPU
AccessioniPrimary (citable) accession number: L1LUV4
Entry historyiIntegrated into UniProtKB/TrEMBL: March 6, 2013
Last sequence update: March 6, 2013
Last modified: June 7, 2017
This is version 34 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported