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L0HEQ2

- L0HEQ2_METFS

UniProt

L0HEQ2 - L0HEQ2_METFS

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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Methanoregula formicica (strain DSM 22288 / NBRC 105244 / SMSP)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei152 – 1521Proton acceptorUniRule annotation
Binding sitei154 – 1541SubstrateUniRule annotation
Metal bindingi178 – 1781Magnesium; via carbamate groupUniRule annotation
Metal bindingi180 – 1801MagnesiumUniRule annotation
Metal bindingi181 – 1811MagnesiumUniRule annotation
Active sitei270 – 2701Proton acceptorUniRule annotation
Binding sitei271 – 2711SubstrateUniRule annotation
Binding sitei303 – 3031SubstrateUniRule annotation
Sitei310 – 3101Transition state stabilizerUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. oxidoreductase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. AMP catabolic process Source: UniProtKB-HAMAP
  2. carbon fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:Metfor_2181Imported
OrganismiMethanoregula formicica (strain DSM 22288 / NBRC 105244 / SMSP)Imported
Taxonomic identifieri593750 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
ProteomesiUP000010824: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni353 – 3553Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family.UniRule annotation
Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
InterProiIPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

L0HEQ2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAIDWYKEFV DLKYEPAKDD LVCLFYFEPA AGVSGREAAG RIASESSTGT
60 70 80 90 100
WTTLFSMPPR MKALQATAFH IEGNVVKIAY PIELWEEGNT VQLMSGIAGN
110 120 130 140 150
IFGMKAVRNL RLIDVALPPA YLKHFQGPHF GNDGIRKMMG IQKRPLTGAV
160 170 180 190 200
PKPKIGFSAK EHADIGYETW MGGFDFVKDD ENLTSTSFNK FEDRVKYMTK
210 220 230 240 250
LRDKAEKETG EKKSAFLNIT ADVETMKKRA DLLAANGWNY CMIDVVVAGT
260 270 280 290 300
AAVMTMRDYC SDLGLAIHAH RAMHAALDRN RKHGISMQFL AKIMRLIGVS
310 320 330 340 350
QIHTGTAVGK LTGSRKESLA LANLLREKKT AAVEGMLLEQ DWGKIKSAFP
360 370 380 390 400
VSSGGLHPGL VPDVMDIYGP EMVLLVSGGI HGHPKGTRAG AKATMQAMEA
410 420 430
WQEGVSLEEK AKKAKELKEA LGKWGHYKPI
Length:430
Mass (Da):47,321
Last modified:March 6, 2013 - v1
Checksum:i80EBCB6EF255B07F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003167 Genomic DNA. Translation: AGB03187.1.
RefSeqiYP_007249694.1. NC_019943.1.

Genome annotation databases

EnsemblBacteriaiAGB03187; AGB03187; Metfor_2181.
GeneIDi14309573.
KEGGimfo:Metfor_2181.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003167 Genomic DNA. Translation: AGB03187.1 .
RefSeqi YP_007249694.1. NC_019943.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AGB03187 ; AGB03187 ; Metfor_2181 .
GeneIDi 14309573.
KEGGi mfo:Metfor_2181.

Phylogenomic databases

KOi K01601.

Family and domain databases

Gene3Di 3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
InterProi IPR017712. RuBisCO_III.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view ]
Pfami PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
PROSITEi PS00157. RUBISCO_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 22288 / NBRC 105244 / SMSPImported.

Entry informationi

Entry nameiL0HEQ2_METFS
AccessioniPrimary (citable) accession number: L0HEQ2
Entry historyi
Integrated into UniProtKB/TrEMBL: March 6, 2013
Last sequence update: March 6, 2013
Last modified: October 29, 2014
This is version 10 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3