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K9Z349 (K9Z349_CYAAP) Unreviewed, UniProtKB/TrEMBL

Last modified July 9, 2014. Version 11. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338

Short name=RuBisCO large subunit HAMAP-Rule MF_01338
EC=4.1.1.39 HAMAP-Rule MF_01338
Gene names
Name:cbbL HAMAP-Rule MF_01338
Synonyms:rbcL HAMAP-Rule MF_01338
Ordered Locus Names:Cyan10605_0644 EMBL AFZ52783.1
OrganismCyanobacterium aponinum (strain PCC 10605) [Complete proteome] [HAMAP] EMBL AFZ52783.1
Taxonomic identifier755178 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesCyanobacterium

Protein attributes

Sequence length468 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers By similarity. HAMAP-Rule MF_01338

Post-translational modification

The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover By similarity. HAMAP-Rule MF_01338

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity. HAMAP-Rule MF_01338

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily. HAMAP-Rule MF_01338

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1681Proton acceptor By similarity HAMAP-Rule MF_01338
Active site2871Proton acceptor By similarity HAMAP-Rule MF_01338
Metal binding1941Magnesium; via carbamate group By similarity HAMAP-Rule MF_01338
Metal binding1961Magnesium By similarity HAMAP-Rule MF_01338
Metal binding1971Magnesium By similarity HAMAP-Rule MF_01338
Binding site1161Substrate; in homodimeric partner By similarity HAMAP-Rule MF_01338
Binding site1661Substrate By similarity HAMAP-Rule MF_01338
Binding site1701Substrate By similarity HAMAP-Rule MF_01338
Binding site2881Substrate By similarity HAMAP-Rule MF_01338
Binding site3201Substrate By similarity HAMAP-Rule MF_01338
Binding site3721Substrate By similarity HAMAP-Rule MF_01338
Site3271Transition state stabilizer By similarity HAMAP-Rule MF_01338

Amino acid modifications

Modified residue1941N6-carboxylysine By similarity HAMAP-Rule MF_01338
Disulfide bond240Interchain; in linked form By similarity HAMAP-Rule MF_01338

Sequences

Sequence LengthMass (Da)Tools
K9Z349 [UniParc].

Last modified March 6, 2013. Version 1.
Checksum: C6002D758ABF66F5

FASTA46852,005
        10         20         30         40         50         60 
MAQAGFKAGV QDYRLTYYTP DYTPKDTDLL ACFRMTPQPG VPPEECAAAV AAESSTGTWT 

        70         80         90        100        110        120 
TVWTDGLTDL DRYKGRCYSV EPVPGEDNQY FCFVAYPLDL FEEGSITNVL TSLVGNVFGF 

       130        140        150        160        170        180 
KALRALRLED IRFPVALIKT YQGPPHGITV ERDLLNKYGR PLLGCTIKPK LGLSAKNYGR 

       190        200        210        220        230        240 
AVYECLRGGL DFTKDDENIN SQPFMRWRDR FLFVQEAIEK AQAETNEIKG HYLNVTAGTC 

       250        260        270        280        290        300 
EEMLKRAEFA KEIGTPIIMH DFLTGGFTAN TTLAKWCRDN GVLLHIHRAM HAVIDRQKNH 

       310        320        330        340        350        360 
GIHFRVLAKC LRLSGGDHLH SGTVVGKLEG DRAATLGFVD LMREDYVEED RSRGVFFTQD 

       370        380        390        400        410        420 
YASLPGTMPV ASGGIHVWHM PALVEIFGDD SCLQFGGGTL GHPWGNAPGA TANRVALEAC 

       430        440        450        460 
VQARNEGRSL AREGNEVIRE ACRWSPELAA ACELWKEIKF EFDTVDTL 

« Hide

References

[1]"Improving the coverage of the cyanobacterial phylum using diversity-driven genome sequencing."
Shih P.M., Wu D., Latifi A., Axen S.D., Fewer D.P., Talla E., Calteau A., Cai F., Tandeau de Marsac N., Rippka R., Herdman M., Sivonen K., Coursin T., Laurent T., Goodwin L., Nolan M., Davenport K.W., Han C.S. expand/collapse author list , Rubin E.M., Eisen J.A., Woyke T., Gugger M., Kerfeld C.A.
Proc. Natl. Acad. Sci. U.S.A. 110:1053-1058(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 10605.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003947 Genomic DNA. Translation: AFZ52783.1.
RefSeqYP_007160827.1. NC_019776.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFZ52783; AFZ52783; Cyan10605_0644.
GeneID14193067.
KEGGcan:Cyan10605_0644.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01601.
OMAFTQDWAS.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK9Z349_CYAAP
AccessionPrimary (citable) accession number: K9Z349
Entry history
Integrated into UniProtKB/TrEMBL: March 6, 2013
Last sequence update: March 6, 2013
Last modified: July 9, 2014
This is version 11 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)