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K9RV09 (K9RV09_SYNP3) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 8. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase large chain HAMAP-Rule MF_01338

Short name=RuBisCO large subunit HAMAP-Rule MF_01338
EC=4.1.1.39 HAMAP-Rule MF_01338
Gene names
Name:cbbL HAMAP-Rule MF_01338
Synonyms:rbcL HAMAP-Rule MF_01338
Ordered Locus Names:Syn6312_2007 EMBL AFY61139.1
OrganismSynechococcus sp. (strain ATCC 27167 / PCC 6312) [Complete proteome] [HAMAP] EMBL AFY61139.1
Taxonomic identifier195253 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site By similarity. HAMAP-Rule MF_01338

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. HAMAP-Rule MF_01338

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2. HAMAP-Rule MF_01338

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_01338

Subunit structure

Heterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers By similarity. HAMAP-Rule MF_01338

Post-translational modification

The disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover By similarity. HAMAP-Rule MF_01338

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel" By similarity. HAMAP-Rule MF_01338

Sequence similarities

Belongs to the RuBisCO large chain family. Type I subfamily. HAMAP-Rule MF_01338

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site1751Proton acceptor By similarity HAMAP-Rule MF_01338
Active site2941Proton acceptor By similarity HAMAP-Rule MF_01338
Metal binding2011Magnesium; via carbamate group By similarity HAMAP-Rule MF_01338
Metal binding2031Magnesium By similarity HAMAP-Rule MF_01338
Metal binding2041Magnesium By similarity HAMAP-Rule MF_01338
Binding site1231Substrate; in homodimeric partner By similarity HAMAP-Rule MF_01338
Binding site1731Substrate By similarity HAMAP-Rule MF_01338
Binding site1771Substrate By similarity HAMAP-Rule MF_01338
Binding site2951Substrate By similarity HAMAP-Rule MF_01338
Binding site3271Substrate By similarity HAMAP-Rule MF_01338
Binding site3791Substrate By similarity HAMAP-Rule MF_01338
Site3341Transition state stabilizer By similarity HAMAP-Rule MF_01338

Amino acid modifications

Modified residue2011N6-carboxylysine By similarity HAMAP-Rule MF_01338
Disulfide bond247Interchain; in linked form By similarity HAMAP-Rule MF_01338

Sequences

Sequence LengthMass (Da)Tools
K9RV09 [UniParc].

Last modified March 6, 2013. Version 1.
Checksum: BF456F1B027C9C50

FASTA47552,773
        10         20         30         40         50         60 
MSYAQKAPGK AGYKAGVQDY RLTYYTPDYT PKDTDILAAF RVTPQPGVPY EEAAAAVAAE 

        70         80         90        100        110        120 
SSTGTWTTVW TDLLTDLDRY KGRCYDIESV PGEDNQFIAY IAYPLDLFEE GSVTNLLTSL 

       130        140        150        160        170        180 
VGNVFGFKAL KALRLEDLRI PVAYLKTFQG PPHGIQVERD KINKYGRPLL GCTIKPKLGL 

       190        200        210        220        230        240 
SAKNYGRAVY ECLRGGLDFT KDDENINSQP FQRWRDRFLF VADAIHKAQA ETGEVKGHYL 

       250        260        270        280        290        300 
NVTAATCEEM LKRAEFAKEL EMPIIMHDFL TGGFTANTTL AHWCRDNGVL LHIHRAMHAV 

       310        320        330        340        350        360 
IDRQKNHGIH FRVLAKCLRM SGGDHIHTGT VVGKLEGDKA ITLGFIDLLR ENYIEQDRSR 

       370        380        390        400        410        420 
GVYFTQDWAS MGGVMAVASG GIHVWHMPAL VEIFGDDSVL QFGGGTLGHP WGNAPGATAN 

       430        440        450        460        470 
RVALEACVQA RNEGRDLMRE GGDIIREACK WSPELAAACE LWKEIKFEFE AVDTV 

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References

[1]"Improving the coverage of the cyanobacterial phylum using diversity-driven genome sequencing."
Shih P.M., Wu D., Latifi A., Axen S.D., Fewer D.P., Talla E., Calteau A., Cai F., Tandeau de Marsac N., Rippka R., Herdman M., Sivonen K., Coursin T., Laurent T., Goodwin L., Nolan M., Davenport K.W., Han C.S. expand/collapse author list , Rubin E.M., Eisen J.A., Woyke T., Gugger M., Kerfeld C.A.
Proc. Natl. Acad. Sci. U.S.A. 110:1053-1058(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27167 / PCC 6312.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003558 Genomic DNA. Translation: AFY61139.1.
RefSeqYP_007061682.1. NC_019680.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFY61139; AFY61139; Syn6312_2007.
GeneID14076217.
KEGGsyne:Syn6312_2007.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01601.

Family and domain databases

Gene3D3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPMF_01338. RuBisCO_L_type1.
InterProIPR020878. RuBisCo_large_chain_AS.
IPR020888. RuBisCO_lsu.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK9RV09_SYNP3
AccessionPrimary (citable) accession number: K9RV09
Entry history
Integrated into UniProtKB/TrEMBL: March 6, 2013
Last sequence update: March 6, 2013
Last modified: February 19, 2014
This is version 8 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)