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Protein

Ribulose bisphosphate carboxylase large chain

Gene

cbbL

Organism
Cyanobium gracile (strain ATCC 27147 / PCC 6307)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei115Substrate; in homodimeric partnerUniRule annotation1
Binding sitei165SubstrateUniRule annotation1
Active sitei167Proton acceptorUniRule annotation1
Binding sitei169SubstrateUniRule annotation1
Metal bindingi193Magnesium; via carbamate groupUniRule annotation1
Metal bindingi195MagnesiumUniRule annotation1
Metal bindingi196MagnesiumUniRule annotation1
Active sitei286Proton acceptorUniRule annotation1
Binding sitei287SubstrateUniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Sitei326Transition state stabilizerUniRule annotation1
Binding sitei371SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, MonooxygenaseUniRule annotation, Oxidoreductase

Keywords - Biological processi

Calvin cycleUniRule annotation, Carbon dioxide fixationUniRule annotation, PhotorespirationUniRule annotation, PhotosynthesisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase large chainUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCO large subunitUniRule annotation
Gene namesi
Name:cbbLUniRule annotation
Synonyms:rbcLUniRule annotation
Ordered Locus Names:Cyagr_0014Imported
OrganismiCyanobium gracile (strain ATCC 27147 / PCC 6307)Imported
Taxonomic identifieri292564 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeCyanobium
Proteomesi
  • UP000010388 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei193N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Heterohexadecamer of 8 large chains and 8 small chains.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliK9P2B9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 136RuBisCO_large_NInterPro annotationAdd BLAST122
Domaini146 – 454RuBisCO_largeInterPro annotationAdd BLAST309

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type I subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.
OMAiFTQDWAS.
OrthoDBiPOG091H14UZ.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

K9P2B9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKKYDAGVK EYRDTYWTPD YVPLDTDLLA CFKCTGQEGV PKEEVAAAVA
60 70 80 90 100
AESSTGTWST VWSELLTDLD FYKGRCYRIE DVPGDKESFY AFIAYPLDLF
110 120 130 140 150
EEGSVTNVLT SLVGNVFGFK ALRHLRLEDI RFPMAFIKTC PGPPNGIHVE
160 170 180 190 200
RDRMNKYGRP LLGCTIKPKL GLSGKNYGRV VYECLRGGLD FTKDDENINS
210 220 230 240 250
QPFQRWQNRF EFVAEAVQLA QEETGEKKGH YLNCTAATPE EMYERAEFAK
260 270 280 290 300
ELGQPIIMHD YITGGFTANT GLSKWCRKNG MLLHIHRAMH AVIDRHPKHG
310 320 330 340 350
IHFRVLAKCL RLSGGDQLHT GTVVGKLEGD RQTTLGFIDQ LRESFIPEDR
360 370 380 390 400
TRGNFFDQDW GSMPGVFAVA SGGIHVWHMP ALVAIFGDDS VLQFGGGTHG
410 420 430 440 450
HPWGSAAGAA ANRVALEACV KARNAGREIE KEGRDILTEA AKHSPELAIA
460 470
LETWKEIKFE FDTVDKLDVG
Length:470
Mass (Da):52,449
Last modified:March 6, 2013 - v1
Checksum:i8E9B54246EC92F04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003495 Genomic DNA. Translation: AFY27235.1.
RefSeqiWP_015107694.1. NC_019675.1.

Genome annotation databases

EnsemblBacteriaiAFY27235; AFY27235; Cyagr_0014.
KEGGicgc:Cyagr_0014.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003495 Genomic DNA. Translation: AFY27235.1.
RefSeqiWP_015107694.1. NC_019675.1.

3D structure databases

ProteinModelPortaliK9P2B9.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAFY27235; AFY27235; Cyagr_0014.
KEGGicgc:Cyagr_0014.

Phylogenomic databases

KOiK01601.
OMAiFTQDWAS.
OrthoDBiPOG091H14UZ.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01338. RuBisCO_L_type1. 1 hit.
InterProiIPR033966. RuBisCO.
IPR020878. RuBisCo_large_chain_AS.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR020888. RuBisCO_lsuI.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
PROSITEiPS00157. RUBISCO_LARGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiK9P2B9_CYAGP
AccessioniPrimary (citable) accession number: K9P2B9
Entry historyi
Integrated into UniProtKB/TrEMBL: March 6, 2013
Last sequence update: March 6, 2013
Last modified: November 30, 2016
This is version 28 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

The basic functional RuBisCO is composed of a large chain homodimer in a "head-to-tail" conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric "cap" on each end of the "barrel".UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.