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K9N7C7 (R1AB_CVEMC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Replicase polyprotein 1ab

Short name=pp1ab
Alternative name(s):
ORF1ab polyprotein

Cleaved into the following 15 chains:

  1. Non-structural protein 1
    Short name=nsp1
    Alternative name(s):
    Leader protein
  2. Non-structural protein 2
    Short name=nsp2
    Alternative name(s):
    p65 homolog
  3. Non-structural protein 3
    Short name=nsp3
    EC=3.4.19.12
    EC=3.4.22.69
    Alternative name(s):
    PL2-PRO
    Papain-like proteinase
    Short name=PL-PRO
    SARS coronavirus main proteinase
  4. Non-structural protein 4
    Short name=nsp4
  5. 3C-like proteinase
    Short name=3CL-PRO
    Short name=3CLp
    EC=3.4.22.-
    Alternative name(s):
    nsp5
  6. Non-structural protein 6
    Short name=nsp6
  7. Non-structural protein 7
    Short name=nsp7
  8. Non-structural protein 8
    Short name=nsp8
  9. Non-structural protein 9
    Short name=nsp9
  10. Non-structural protein 10
    Short name=nsp10
    Alternative name(s):
    Growth factor-like peptide
    Short name=GFL
  11. RNA-directed RNA polymerase
    Short name=Pol
    Short name=RdRp
    EC=2.7.7.48
    Alternative name(s):
    nsp12
  12. Helicase
    Short name=Hel
    EC=3.6.4.12
    EC=3.6.4.13
    Alternative name(s):
    nsp13
  13. Exoribonuclease
    Short name=ExoN
    EC=3.1.13.-
    Alternative name(s):
    nsp14
  14. Uridylate-specific endoribonuclease
    EC=3.1.-.-
    Alternative name(s):
    NendoU
    nsp15
  15. Putative 2'-O-methyl transferase
    EC=2.1.1.-
    Alternative name(s):
    nsp16
Gene names
Name:rep
ORF Names:1a-1b
OrganismHuman coronavirus EMC (isolate United Kingdom/H123990006/2012) (HCoV-EMC)
Taxonomic identifier1263720 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNidoviralesCoronaviridaeCoronavirinaeBetacoronavirusunclassified Betacoronavirus

Protein attributes

Sequence length7078 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products By similarity.

The papain-like proteinase (PL-PRO) is responsible for the cleavages located at the N-terminus of replicase polyprotein. In addition, PL-PRO possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. Antagonizes innate immune induction of type I interferon by blocking the phosphorylation, dimerization and subsequent nuclear translocation of host IRF-3 By similarity.

The main proteinase 3CL-PRO is responsible for the majority of cleavages as it cleaves the C-terminus of replicase polyprotein at 11 sites By similarity.

The helicase which contains a zinc finger structure displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Its ATPase activity is strongly stimulated by poly(U), poly(dT), poly(C), poly(dA), but not by poly(G) By similarity.

The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction By similarity.

Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter By similarity.

Nsp9 is a ssRNA-binding protein By similarity.

NendoU is a Mn2+-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond By similarity.

Non-structural protein 1: binds to the 40S ribosomal subunit and inhibits host translation. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response By similarity.

Catalytic activity

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

ATP + H2O = ADP + phosphate.

TSAVLQ-|-SGFRK-NH2 and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

3CL-PRO exists as monomer and homodimer. Only the homodimer shows catalytic activity. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer By similarity.

Subcellular location

Non-structural protein 3: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 4: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 6: Host membrane; Multi-pass membrane protein Potential.

Non-structural protein 7: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 8: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 9: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Non-structural protein 10: Host cytoplasmhost perinuclear region By similarity. Note: nsp7, nsp8, nsp9 and nsp10 are localized in cytoplasmic foci, largely perinuclear. Late in infection, they merge into confluent complexes By similarity.

Helicase: Host endoplasmic reticulum-Golgi intermediate compartment Potential. Note: The helicase interacts with the N protein in membranous complexes and colocalizes with sites of synthesis of new viral RNA By similarity.

Uridylate-specific endoribonuclease: Host cytoplasmhost perinuclear region By similarity.

Domain

The hydrophobic domains (HD) could mediate the membrane association of the replication complex and thereby alter the architecture of the host cell membrane.

Post-translational modification

Specific enzymatic cleavages in vivo by its own proteases yield mature proteins. 3CL-PRO and PL-PRO proteinases are autocatalytically processed By similarity.

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Contains 1 Macro domain.

Contains 1 peptidase C16 domain.

Contains 1 peptidase C30 domain.

Contains 1 RdRp catalytic domain.

Ontologies

Keywords
   Biological processActivation of host autophagy by virus
Decay of host mRNAs by virus
Host gene expression shutoff by virus
Host mRNA suppression by virus
Host translation shutoff by virus
Host-virus interaction
Inhibition of host innate immune response by virus
Inhibition of host interferon signaling pathway by virus
Inhibition of host ISG15 by virus
Modulation of host ubiquitin pathway by viral deubiquitinase
Modulation of host ubiquitin pathway by virus
Ubl conjugation pathway
Viral immunoevasion
Viral RNA replication
   Cellular componentHost cytoplasm
Host membrane
Membrane
   Coding sequence diversityRibosomal frameshifting
   DomainRepeat
Transmembrane
Transmembrane helix
Zinc-finger
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
   Molecular functionEndonuclease
Exonuclease
Helicase
Hydrolase
Nuclease
Nucleotidyltransferase
Protease
RNA-directed RNA polymerase
Thiol protease
Transferase
Gene Ontology (GO)
   Biological_processinduction by virus of catabolism of host mRNA

Inferred from electronic annotation. Source: UniProtKB-KW

induction by virus of host autophagy

Inferred from electronic annotation. Source: UniProtKB-KW

modulation by virus of host protein ubiquitination

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host ISG15 activity

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host translation

Inferred from electronic annotation. Source: UniProtKB-KW

suppression by virus of host type I interferon-mediated signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

transcription, DNA-templated

Inferred from electronic annotation. Source: InterPro

viral RNA genome replication

Inferred from electronic annotation. Source: InterPro

viral protein processing

Inferred from electronic annotation. Source: InterPro

   Cellular_componenthost cell endoplasmic reticulum-Golgi intermediate compartment

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

host cell perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

RNA-directed RNA polymerase activity

Inferred from electronic annotation. Source: UniProtKB-KW

cysteine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

endonuclease activity

Inferred from electronic annotation. Source: UniProtKB-KW

exoribonuclease activity, producing 5'-phosphomonoesters

Inferred from electronic annotation. Source: InterPro

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

methyltransferase activity

Inferred from electronic annotation. Source: InterPro

omega peptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]
Isoform Replicase polyprotein 1ab (identifier: K9N7C7-1)

Also known as: pp1ab;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: Produced by -1 ribosomal frameshifting at the 1a-1b genes boundary.
Isoform Replicase polyprotein 1a (identifier: K9N638-1)

Also known as: pp1a; ORF1a polyprotein;

The sequence of this isoform can be found in the external entry K9N638.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193Non-structural protein 1 By similarity
PRO_0000422439
Chain194 – 853660Non-structural protein 2 By similarity
PRO_0000422440
Chain854 – 27401887Non-structural protein 3 By similarity
PRO_0000422441
Chain2741 – 3247507Non-structural protein 4 Potential
PRO_0000422442
Chain3248 – 35533063C-like proteinase By similarity
PRO_0000422443
Chain3554 – 3845292Non-structural protein 6 By similarity
PRO_0000422444
Chain3846 – 392883Non-structural protein 7 By similarity
PRO_0000422445
Chain3929 – 4127199Non-structural protein 8 By similarity
PRO_0000422446
Chain4128 – 4237110Non-structural protein 9 By similarity
PRO_0000422447
Chain4238 – 4377140Non-structural protein 10 By similarity
PRO_0000422448
Chain4378 – 5310933RNA-directed RNA polymerase By similarity
PRO_0000422449
Chain5311 – 5908598Helicase By similarity
PRO_0000422450
Chain5909 – 6432524Exoribonuclease By similarity
PRO_0000422451
Chain6433 – 6775343Uridylate-specific endoribonuclease By similarity
PRO_0000422452
Chain6776 – 7078303Putative 2'-O-methyl transferase By similarity
PRO_0000422453

Regions

Transmembrane2105 – 212521Helical; Potential
Transmembrane2177 – 219721Helical; Potential
Transmembrane2281 – 230121Helical; Potential
Transmembrane2305 – 232521Helical; Potential
Transmembrane2330 – 235021Helical; Potential
Transmembrane2757 – 277721Helical; Potential
Transmembrane3028 – 304821Helical; Potential
Transmembrane3062 – 308221Helical; Potential
Transmembrane3104 – 312421Helical; Potential
Transmembrane3125 – 314521Helical; Potential
Transmembrane3559 – 357921Helical; Potential
Transmembrane3593 – 361321Helical; Potential
Transmembrane3618 – 363821Helical; Potential
Transmembrane3664 – 368421Helical; Potential
Transmembrane3691 – 371121Helical; Potential
Transmembrane3740 – 376021Helical; Potential
Transmembrane3765 – 378521Helical; Potential
Domain1110 – 1276167Macro
Domain1552 – 1823272Peptidase C16
Domain3248 – 3553306Peptidase C30
Domain4990 – 5152163RdRp catalytic
Zinc finger1672 – 170938C4-type
Zinc finger4311 – 432717
Zinc finger4354 – 436714
Nucleotide binding5592 – 55998ATP By similarity
Region2040 – 2363324HD1
Region2761 – 3171411HD2
Region3571 – 3785215HD3

Sites

Active site15921For PL-PRO activity By similarity
Active site17591For PL-PRO activity By similarity
Active site32881For 3CL-PRO activity By similarity
Active site33951For 3CL-PRO activity By similarity
Metal binding43111Zinc
Metal binding43141Zinc
Metal binding43201Zinc
Metal binding43271Zinc
Metal binding43541Zinc
Metal binding43571Zinc
Metal binding43651Zinc
Site193 – 1942Cleavage By similarity
Site853 – 8542Cleavage; by PL-PRO By similarity
Site2740 – 27412Cleavage; by PL-PRO By similarity
Site3247 – 32482Cleavage; by 3CL-PRO By similarity
Site3553 – 35542Cleavage; by 3CL-PRO By similarity
Site3845 – 38462Cleavage; by 3CL-PRO By similarity
Site3928 – 39292Cleavage; by 3CL-PRO By similarity
Site4127 – 41282Cleavage; by 3CL-PRO By similarity
Site4237 – 42382Cleavage; by 3CL-PRO By similarity
Site4377 – 43782Cleavage; by 3CL-PRO By similarity
Site5310 – 53112Cleavage; by 3CL-PRO By similarity
Site5908 – 59092Cleavage; by 3CL-PRO By similarity
Site6432 – 64332Cleavage; by 3CL-PRO By similarity
Site6775 – 67762Cleavage; by 3CL-PRO By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform Replicase polyprotein 1ab (pp1ab) [UniParc].

Last modified March 6, 2013. Version 1.
Checksum: A944AF691D57A1E0

FASTA7,078789,563
        10         20         30         40         50         60 
MSFVAGVTAQ GARGTYRAAL NSEKHQDHVS LTVPLCGSGN LVEKLSPWFM DGENAYEVVK 

        70         80         90        100        110        120 
AMLLKKEPLL YVPIRLAGHT RHLPGPRVYL VERLIACENP FMVNQLAYSS SANGSLVGTT 

       130        140        150        160        170        180 
LQGKPIGMFF PYDIELVTGK QNILLRKYGR GGYHYTPFHY ERDNTSCPEW MDDFEADPKG 

       190        200        210        220        230        240 
KYAQNLLKKL IGGDVTPVDQ YMCGVDGKPI SAYAFLMAKD GITKLADVEA DVAARADDEG 

       250        260        270        280        290        300 
FITLKNNLYR LVWHVERKDV PYPKQSIFTI NSVVQKDGVE NTPPHYFTLG CKILTLTPRN 

       310        320        330        340        350        360 
KWSGVSDLSL KQKLLYTFYG KESLENPTYI YHSAFIECGS CGNDSWLTGN AIQGFACGCG 

       370        380        390        400        410        420 
ASYTANDVEV QSSGMIKPNA LLCATCPFAK GDSCSSNCKH SVAQLVSYLS ERCNVIADSK 

       430        440        450        460        470        480 
SFTLIFGGVA YAYFGCEEGT MYFVPRAKSV VSRIGDSIFT GCTGSWNKVT QIANMFLEQT 

       490        500        510        520        530        540 
QHSLNFVGEF VVNDVVLAIL SGTTTNVDKI RQLLKGVTLD KLRDYLADYD VAVTAGPFMD 

       550        560        570        580        590        600 
NAINVGGTGL QYAAITAPYV VLTGLGESFK KVATIPYKVC NSVKDTLTYY AHSVLYRVFP 

       610        620        630        640        650        660 
YDMDSGVSSF SELLFDCVDL SVASTYFLVR LLQDKTGDFM STIITSCQTA VSKLLDTCFE 

       670        680        690        700        710        720 
ATEATFNFLL DLAGLFRIFL RNAYVYTSQG FVVVNGKVST LVKQVLDLLN KGMQLLHTKV 

       730        740        750        760        770        780 
SWAGSNISAV IYSGRESLIF PSGTYYCVTT KAKSVQQDLD VILPGEFSKK QLGLLQPTDN 

       790        800        810        820        830        840 
STTVSVTVSS NMVETVVGQL EQTNMHSPDV IVGDYVIISE KLFVRSKEED GFAFYPACTN 

       850        860        870        880        890        900 
GHAVPTLFRL KGGAPVKKVA FGGDQVHEVA AVRSVTVEYN IHAVLDTLLA SSSLRTFVVD 

       910        920        930        940        950        960 
KSLSIEEFAD VVKEQVSDLL VKLLRGMPIP DFDLDDFIDA PCYCFNAEGD ASWSSTMIFS 

       970        980        990       1000       1010       1020 
LHPVECDEEC SEVEASDLEE GESECISETS TEQVDVSHEI SDDEWAAAVD EAFPLDEAED 

      1030       1040       1050       1060       1070       1080 
VTESVQEEAQ PVEVPVEDIA QVVIADTLQE TPVVSDTVEV PPQVVKLPSE PQTIQPEVKE 

      1090       1100       1110       1120       1130       1140 
VAPVYEADTE QTQSVTVKPK RLRKKRNVDP LSNFEHKVIT ECVTIVLGDA IQVAKCYGES 

      1150       1160       1170       1180       1190       1200 
VLVNAANTHL KHGGGIAGAI NAASKGAVQK ESDEYILAKG PLQVGDSVLL QGHSLAKNIL 

      1210       1220       1230       1240       1250       1260 
HVVGPDARAK QDVSLLSKCY KAMNAYPLVV TPLVSAGIFG VKPAVSFDYL IREAKTRVLV 

      1270       1280       1290       1300       1310       1320 
VVNSQDVYKS LTIVDIPQSL TFSYDGLRGA IRKAKDYGFT VFVCTDNSAN TKVLRNKGVD 

      1330       1340       1350       1360       1370       1380 
YTKKFLTVDG VQYYCYTSKD TLDDILQQAN KSVGIISMPL GYVSHGLDLI QAGSVVRRVN 

      1390       1400       1410       1420       1430       1440 
VPYVCLLANK EQEAILMSED VKLNPSEDFI KHVRTNGGYN SWHLVEGELL VQDLRLNKLL 

      1450       1460       1470       1480       1490       1500 
HWSDQTICYK DSVFYVVKNS TAFPFETLSA CRAYLDSRTT QQLTIEVLVT VDGVNFRTVV 

      1510       1520       1530       1540       1550       1560 
LNNKNTYRSQ LGCVFFNGAD ISDTIPDEKQ NGHSLYLADN LTADETKALK ELYGPVDPTF 

      1570       1580       1590       1600       1610       1620 
LHRFYSLKAA VHKWKMVVCD KVRSLKLSDN NCYLNAVIMT LDLLKDIKFV IPALQHAFMK 

      1630       1640       1650       1660       1670       1680 
HKGGDSTDFI ALIMAYGNCT FGAPDDASRL LHTVLAKAEL CCSARMVWRE WCNVCGIKDV 

      1690       1700       1710       1720       1730       1740 
VLQGLKACCY VGVQTVEDLR ARMTYVCQCG GERHRQIVEH TTPWLLLSGT PNEKLVTTST 

      1750       1760       1770       1780       1790       1800 
APDFVAFNVF QGIETAVGHY VHARLKGGLI LKFDSGTVSK TSDWKCKVTD VLFPGQKYSS 

      1810       1820       1830       1840       1850       1860 
DCNVVRYSLD GNFRTEVDPD LSAFYVKDGK YFTSEPPVTY SPATILAGSV YTNSCLVSSD 

      1870       1880       1890       1900       1910       1920 
GQPGGDAISL SFNNLLGFDS SKPVTKKYTY SFLPKEDGDV LLAEFDTYDP IYKNGAMYKG 

      1930       1940       1950       1960       1970       1980 
KPILWVNKAS YDTNLNKFNR ASLRQIFDVA PIELENKFTP LSVESTPVEP PTVDVVALQQ 

      1990       2000       2010       2020       2030       2040 
EMTIVKCKGL NKPFVKDNVS FVADDSGTPV VEYLSKEDLH TLYVDPKYQV IVLKDNVLSS 

      2050       2060       2070       2080       2090       2100 
MLRLHTVESG DINVVAASGS LTRKVKLLFR ASFYFKEFAT RTFTATTAVG SCIKSVVRHL 

      2110       2120       2130       2140       2150       2160 
GVTKGILTGC FSFVKMLFML PLAYFSDSKL GTTEVKVSAL KTAGVVTGNV VKQCCTAAVD 

      2170       2180       2190       2200       2210       2220 
LSMDKLRRVD WKSTLRLLLM LCTTMVLLSS VYHLYVFNQV LSSDVMFEDA QGLKKFYKEV 

      2230       2240       2250       2260       2270       2280 
RAYLGISSAC DGLASAYRAN SFDVPTFCAN RSAMCNWCLI SQDSITHYPA LKMVQTHLSH 

      2290       2300       2310       2320       2330       2340 
YVLNIDWLWF AFETGLAYML YTSAFNWLLL AGTLHYFFAQ TSIFVDWRSY NYAVSSAFWL 

      2350       2360       2370       2380       2390       2400 
FTHIPMAGLV RMYNLLACLW LLRKFYQHVI NGCKDTACLL CYKRNRLTRV EASTVVCGGK 

      2410       2420       2430       2440       2450       2460 
RTFYITANGG ISFCRRHNWN CVDCDTAGVG NTFICEEVAN DLTTALRRPI NATDRSHYYV 

      2470       2480       2490       2500       2510       2520 
DSVTVKETVV QFNYRRDGQP FYERFPLCAF TNLDKLKFKE VCKTTTGIPE YNFIIYDSSD 

      2530       2540       2550       2560       2570       2580 
RGQESLARSA CVYYSQVLCK SILLVDSSLV TSVGDSSEIA TKMFDSFVNS FVSLYNVTRD 

      2590       2600       2610       2620       2630       2640 
KLEKLISTAR DGVRRGDNFH SVLTTFIDAA RGPAGVESDV ETNEIVDSVQ YAHKHDIQIT 

      2650       2660       2670       2680       2690       2700 
NESYNNYVPS YVKPDSVSTS DLGSLIDCNA ASVNQIVLRN SNGACIWNAA AYMKLSDALK 

      2710       2720       2730       2740       2750       2760 
RQIRIACRKC NLAFRLTTSK LRANDNILSV RFTANKIVGG APTWFNALRD FTLKGYVLAT 

      2770       2780       2790       2800       2810       2820 
IIVFLCAVLM YLCLPTFSMV PVEFYEDRIL DFKVLDNGII RDVNPDDKCF ANKHRSFTQW 

      2830       2840       2850       2860       2870       2880 
YHEHVGGVYD NSITCPLTVA VIAGVAGARI PDVPTTLAWV NNQIIFFVSR VFANTGSVCY 

      2890       2900       2910       2920       2930       2940 
TPIDEIPYKS FSDSGCILPS ECTMFRDAEG RMTPYCHDPT VLPGAFAYSQ MRPHVRYDLY 

      2950       2960       2970       2980       2990       3000 
DGNMFIKFPE VVFESTLRIT RTLSTQYCRF GSCEYAQEGV CITTNGSWAI FNDHHLNRPG 

      3010       3020       3030       3040       3050       3060 
VYCGSDFIDI VRRLAVSLFQ PITYFQLTTS LVLGIGLCAF LTLLFYYINK VKRAFADYTQ 

      3070       3080       3090       3100       3110       3120 
CAVIAVVAAV LNSLCICFVA SIPLCIVPYT ALYYYATFYF TNEPAFIMHV SWYIMFGPIV 

      3130       3140       3150       3160       3170       3180 
PIWMTCVYTV AMCFRHFFWV LAYFSKKHVE VFTDGKLNCS FQDAASNIFV INKDTYAALR 

      3190       3200       3210       3220       3230       3240 
NSLTNDAYSR FLGLFNKYKY FSGAMETAAY REAAACHLAK ALQTYSETGS DLLYQPPNCS 

      3250       3260       3270       3280       3290       3300 
ITSGVLQSGL VKMSHPSGDV EACMVQVTCG SMTLNGLWLD NTVWCPRHVM CPADQLSDPN 

      3310       3320       3330       3340       3350       3360 
YDALLISMTN HSFSVQKHIG APANLRVVGH AMQGTLLKLT VDVANPSTPA YTFTTVKPGA 

      3370       3380       3390       3400       3410       3420 
AFSVLACYNG RPTGTFTVVM RPNYTIKGSF LCGSCGSVGY TKEGSVINFC YMHQMELANG 

      3430       3440       3450       3460       3470       3480 
THTGSAFDGT MYGAFMDKQV HQVQLTDKYC SVNVVAWLYA AILNGCAWFV KPNRTSVVSF 

      3490       3500       3510       3520       3530       3540 
NEWALANQFT EFVGTQSVDM LAVKTGVAIE QLLYAIQQLY TGFQGKQILG STMLEDEFTP 

      3550       3560       3570       3580       3590       3600 
EDVNMQIMGV VMQSGVRKVT YGTAHWLFAT LVSTYVIILQ ATKFTLWNYL FETIPTQLFP 

      3610       3620       3630       3640       3650       3660 
LLFVTMAFVM LLVKHKHTFL TLFLLPVAIC LTYANIVYEP TTPISSALIA VANWLAPTNA 

      3670       3680       3690       3700       3710       3720 
YMRTTHTDIG VYISMSLVLV IVVKRLYNPS LSNFALALCS GVMWLYTYSI GEASSPIAYL 

      3730       3740       3750       3760       3770       3780 
VFVTTLTSDY TITVFVTVNL AKVCTYAIFA YSPQLTLVFP EVKMILLLYT CLGFMCTCYF 

      3790       3800       3810       3820       3830       3840 
GVFSLLNLKL RAPMGVYDFK VSTQEFRFMT ANNLTAPRNS WEAMALNFKL IGIGGTPCIK 

      3850       3860       3870       3880       3890       3900 
VAAMQSKLTD LKCTSVVLLS VLQQLHLEAN SRAWAFCVKC HNDILAATDP SEAFEKFVSL 

      3910       3920       3930       3940       3950       3960 
FATLMTFSGN VDLDALASDI FDTPSVLQAT LSEFSHLATF AELEAAQKAY QEAMDSGDTS 

      3970       3980       3990       4000       4010       4020 
PQVLKALQKA VNIAKNAYEK DKAVARKLER MADQAMTSMY KQARAEDKKA KIVSAMQTML 

      4030       4040       4050       4060       4070       4080 
FGMIKKLDND VLNGIISNAR NGCIPLSVIP LCASNKLRVV IPDFTVWNQV VTYPSLNYAG 

      4090       4100       4110       4120       4130       4140 
ALWDITVINN VDNEIVKSSD VVDSNENLTW PLVLECTRAS TSAVKLQNNE IKPSGLKTMV 

      4150       4160       4170       4180       4190       4200 
VSAGQEQTNC NTSSLAYYEP VQGRKMLMAL LSDNAYLKWA RVEGKDGFVS VELQPPCKFL 

      4210       4220       4230       4240       4250       4260 
IAGPKGPEIR YLYFVKNLNN LHRGQVLGHI AATVRLQAGS NTEFASNSSV LSLVNFTVDP 

      4270       4280       4290       4300       4310       4320 
QKAYLDFVNA GGAPLTNCVK MLTPKTGTGI AISVKPESTA DQETYGGASV CLYCRAHIEH 

      4330       4340       4350       4360       4370       4380 
PDVSGVCKYK GKFVQIPAQC VRDPVGFCLS NTPCNVCQYW IGYGCNCDSL RQAALPQSKD 

      4390       4400       4410       4420       4430       4440 
SNFLKRVRGS IVNARIEPCS SGLSTDVVFR AFDICNYKAK VAGIGKYYKT NTCRFVELDD 

      4450       4460       4470       4480       4490       4500 
QGHHLDSYFV VKRHTMENYE LEKHCYDLLR DCDAVAPHDF FIFDVDKVKT PHIVRQRLTE 

      4510       4520       4530       4540       4550       4560 
YTMMDLVYAL RHFDQNSEVL KAILVKYGCC DVTYFENKLW FDFVENPSVI GVYHKLGERV 

      4570       4580       4590       4600       4610       4620 
RQAILNTVKF CDHMVKAGLV GVLTLDNQDL NGKWYDFGDF VITQPGSGVA IVDSYYSYLM 

      4630       4640       4650       4660       4670       4680 
PVLSMTDCLA AETHRDCDFN KPLIEWPLTE YDFTDYKVQL FEKYFKYWDQ TYHANCVNCT 

      4690       4700       4710       4720       4730       4740 
DDRCVLHCAN FNVLFAMTMP KTCFGPIVRK IFVDGVPFVV SCGYHYKELG LVMNMDVSLH 

      4750       4760       4770       4780       4790       4800 
RHRLSLKELM MYAADPAMHI ASSNAFLDLR TSCFSVAALT TGLTFQTVRP GNFNQDFYDF 

      4810       4820       4830       4840       4850       4860 
VVSKGFFKEG SSVTLKHFFF AQDGNAAITD YNYYSYNLPT MCDIKQMLFC MEVVNKYFEI 

      4870       4880       4890       4900       4910       4920 
YDGGCLNASE VVVNNLDKSA GHPFNKFGKA RVYYESMSYQ EQDELFAMTK RNVIPTMTQM 

      4930       4940       4950       4960       4970       4980 
NLKYAISAKN RARTVAGVSI LSTMTNRQYH QKMLKSMAAT RGATCVIGTT KFYGGWDFML 

      4990       5000       5010       5020       5030       5040 
KTLYKDVDNP HLMGWDYPKC DRAMPNMCRI FASLILARKH GTCCTTRDRF YRLANECAQV 

      5050       5060       5070       5080       5090       5100 
LSEYVLCGGG YYVKPGGTSS GDATTAYANS VFNILQATTA NVSALMGANG NKIVDKEVKD 

      5110       5120       5130       5140       5150       5160 
MQFDLYVNVY RSTSPDPKFV DKYYAFLNKH FSMMILSDDG VVCYNSDYAA KGYIAGIQNF 

      5170       5180       5190       5200       5210       5220 
KETLYYQNNV FMSEAKCWVE TDLKKGPHEF CSQHTLYIKD GDDGYFLPYP DPSRILSAGC 

      5230       5240       5250       5260       5270       5280 
FVDDIVKTDG TLMVERFVSL AIDAYPLTKH EDIEYQNVFW VYLQYIEKLY KDLTGHMLDS 

      5290       5300       5310       5320       5330       5340 
YSVMLCGDNS AKFWEEAFYR DLYSSPTTLQ AVGSCVVCHS QTSLRCGTCI RRPFLCCKCC 

      5350       5360       5370       5380       5390       5400 
YDHVIATPHK MVLSVSPYVC NAPGCGVSDV TKLYLGGMSY FCVDHRPVCS FPLCANGLVF 

      5410       5420       5430       5440       5450       5460 
GLYKNMCTGS PSIVEFNRLA TCDWTESGDY TLANTTTEPL KLFAAETLRA TEEASKQSYA 

      5470       5480       5490       5500       5510       5520 
IATIKEIVGE RQLLLVWEAG KSKPPLNRNY VFTGYHITKN SKVQLGEYIF ERIDYSDAVS 

      5530       5540       5550       5560       5570       5580 
YKSSTTYKLT VGDIFVLTSH SVATLTAPTI VNQERYVKIT GLYPTITVPE EFASHVANFQ 

      5590       5600       5610       5620       5630       5640 
KSGYSKYVTV QGPPGTGKSH FAIGLAIYYP TARVVYTACS HAAVDALCEK AFKYLNIAKC 

      5650       5660       5670       5680       5690       5700 
SRIIPAKARV ECYDRFKVNE TNSQYLFSTI NALPETSADI LVVDEVSMCT NYDLSIINAR 

      5710       5720       5730       5740       5750       5760 
IKAKHIVYVG DPAQLPAPRT LLTRGTLEPE NFNSVTRLMC NLGPDIFLSM CYRCPKEIVS 

      5770       5780       5790       5800       5810       5820 
TVSALVYNNK LLAKKELSGQ CFKILYKGNV THDASSAINR PQLTFVKNFI TANPAWSKAV 

      5830       5840       5850       5860       5870       5880 
FISPYNSQNA VARSMLGLTT QTVDSSQGSE YQYVIFCQTA DTAHANNINR FNVAITRAQK 

      5890       5900       5910       5920       5930       5940 
GILCVMTSQA LFESLEFTEL SFTNYKLQSQ IVTGLFKDCS RETSGLSPAY APTYVSVDDK 

      5950       5960       5970       5980       5990       6000 
YKTSDELCVN LNLPANVPYS RVISRMGFKL DATVPGYPKL FITREEAVRQ VRSWIGFDVE 

      6010       6020       6030       6040       6050       6060 
GAHASRNACG TNVPLQLGFS TGVNFVVQPV GVVDTEWGNM LTGIAARPPP GEQFKHLVPL 

      6070       6080       6090       6100       6110       6120 
MHKGAAWPIV RRRIVQMLSD TLDKLSDYCT FVCWAHGFEL TSASYFCKIG KEQKCCMCNR 

      6130       6140       6150       6160       6170       6180 
RAAAYSSPLQ SYACWTHSCG YDYVYNPFFV DVQQWGYVGN LATNHDRYCS VHQGAHVASN 

      6190       6200       6210       6220       6230       6240 
DAIMTRCLAI HSCFIERVDW DIEYPYISHE KKLNSCCRIV ERNVVRAALL AGSFDKVYDI 

      6250       6260       6270       6280       6290       6300 
GNPKGIPIVD DPVVDWHYFD AQPLTRKVQQ LFYTEDMASR FADGLCLFWN CNVPKYPNNA 

      6310       6320       6330       6340       6350       6360 
IVCRFDTRVH SEFNLPGCDG GSLYVNKHAF HTPAYDVSAF RDLKPLPFFY YSTTPCEVHG 

      6370       6380       6390       6400       6410       6420 
NGSMIEDIDY VPLKSAVCIT ACNLGGAVCR KHATEYREYM EAYNLVSASG FRLWCYKTFD 

      6430       6440       6450       6460       6470       6480 
IYNLWSTFTK VQGLENIAFN FVKQGHFIGV EGELPVAVVN DKIFTKSGVN DICMFENKTT 

      6490       6500       6510       6520       6530       6540 
LPTNIAFELY AKRAVRSHPD FKLLHNLQAD ICYKFVLWDY ERSNIYGTAT IGVCKYTDID 

      6550       6560       6570       6580       6590       6600 
VNSALNICFD IRDNGSLEKF MSTPNAIFIS DRKIKKYPCM VGPDYAYFNG AIIRDSDVVK 

      6610       6620       6630       6640       6650       6660 
QPVKFYLYKK VNNEFIDPTE CIYTQSRSCS DFLPLSDMEK DFLSFDSDVF IKKYGLENYA 

      6670       6680       6690       6700       6710       6720 
FEHVVYGDFS HTTLGGLHLL IGLYKKQQEG HIIMEEMLKG SSTIHNYFIT ETNTAAFKAV 

      6730       6740       6750       6760       6770       6780 
CSVIDLKLDD FVMILKSQDL GVVSKVVKVP IDLTMIEFML WCKDGQVQTF YPRLQASADW 

      6790       6800       6810       6820       6830       6840 
KPGHAMPSLF KVQNVNLERC ELANYKQSIP MPRGVHMNIA KYMQLCQYLN TCTLAVPANM 

      6850       6860       6870       6880       6890       6900 
RVIHFGAGSD KGIAPGTSVL RQWLPTDAII IDNDLNEFVS DADITLFGDC VTVRVGQQVD 

      6910       6920       6930       6940       6950       6960 
LVISDMYDPT TKNVTGSNES KALFFTYLCN LINNNLALGG SVAIKITEHS WSVELYELMG 

      6970       6980       6990       7000       7010       7020 
KFAWWTVFCT NANASSSEGF LLGINYLGTI KENIDGGAMH ANYIFWRNST PMNLSTYSLF 

      7030       7040       7050       7060       7070 
DLSKFQLKLK GTPVLQLKES QINELVISLL SQGKLLIRDN DTLSVSTDVL VNTYRKLR 

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Isoform Replicase polyprotein 1a (pp1a) (ORF1a polyprotein) [UniParc].

See K9N638.

References

[1]"Severe respiratory illness caused by a novel coronavirus, in a patient transferred to the United Kingdom from the Middle East, September 2012."
Bermingham A., Chand M.A., Brown C.S., Aarons E., Tong C., Langrish C., Hoschler K., Brown K., Galiano M., Myers R., Pebody R.G., Green H.K., Boddington N.L., Gopal R., Price N., Newsholme W., Drosten C., Fouchier R.A., Zambon M.
Eurosurveillance 17:20290-20290(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
KC164505 Genomic RNA. Translation: AFY13306.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR027351. (+)RNA_virus_helicase_core_dom.
IPR009461. Coronavirus_NSP16.
IPR027352. CV_MBD_dom.
IPR002589. Macro_dom.
IPR009466. NSP11.
IPR024375. Nsp3_coronavir.
IPR014828. NSP7.
IPR014829. NSP8.
IPR014822. NSP9.
IPR027417. P-loop_NTPase.
IPR008740. Peptidase_C30.
IPR013016. Peptidase_C30/C16.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009469. RNA_pol_N_coronovir.
IPR018995. RNA_synth_NSP10_coronavirus.
IPR009003. Trypsin-like_Pept_dom.
IPR014827. Viral_protease.
[Graphical view]
PfamPF06478. Corona_RPol_N. 1 hit.
PF01661. Macro. 1 hit.
PF09401. NSP10. 1 hit.
PF06471. NSP11. 1 hit.
PF06460. NSP13. 1 hit.
PF08716. nsp7. 1 hit.
PF08717. nsp8. 1 hit.
PF08710. nsp9. 1 hit.
PF05409. Peptidase_C30. 1 hit.
PF00680. RdRP_1. 1 hit.
PF11633. SUD-M. 1 hit.
PF08715. Viral_protease. 1 hit.
[Graphical view]
SMARTSM00506. A1pp. 1 hit.
[Graphical view]
SUPFAMSSF101816. SSF101816. 1 hit.
SSF144246. SSF144246. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS51653. CV_MBD. 1 hit.
PS51442. M_PRO. 1 hit.
PS51154. MACRO. 1 hit.
PS51124. PEPTIDASE_C16. 1 hit.
PS51657. PSRV_HELICASE. 1 hit.
PS50507. RDRP_SSRNA_POS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameR1AB_CVEMC
AccessionPrimary (citable) accession number: K9N7C7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: March 6, 2013
Last modified: April 16, 2014
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries