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K4ISX5 (K4ISX5_BIFAP) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 12. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:BAST_1684 EMBL AFU72251.1
OrganismBifidobacterium asteroides (strain PRL2011) [Complete proteome] [HAMAP] EMBL AFU72251.1
Taxonomic identifier1147128 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeBifidobacterialesBifidobacteriaceaeBifidobacterium

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain184 – 391208ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding218 – 27356ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3451Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3581Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3581Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3601Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
K4ISX5 [UniParc].

Last modified January 9, 2013. Version 1.
Checksum: 73FAED88969A8928

FASTA40042,812
        10         20         30         40         50         60 
MAAKHSKTPR QQTGGKTPAV GKTKTTASAT PTTVDSPDST AATTAGEVAK RARRTSHRST 

        70         80         90        100        110        120 
REATPFTMDD VLSLASKATK RADKSLLVIC GGLSHERDVS ISSGHRVQGF LEEAGWTVHV 

       130        140        150        160        170        180 
HDMDGSLLPY LTDESTRPDL VWPLLHGANG EDGSIRDVLE MAELPYIGSR AKASRTAWSK 

       190        200        210        220        230        240 
PIAKNVVRMA GLQTPHSVTL PESMFRELGV EPVIDMLLGS LGLPLFIKPT MGGSAMGCTM 

       250        260        270        280        290        300 
VTEQSQLTQA LINCFAYGDV ALIEQAVTGT EVSVSILEFD GHPLVLPPLE IWTPSGTYDF 

       310        320        330        340        350        360 
EARSTPGPTE FYVPARLDAK TTQAVSDAAL TAHRALGLRD ISRTDFIVDA QGQPQFLESN 

       370        380        390        400 
VAPGMTDTSL LPQSALAAGY NLPELYTALV QSVLNQKRTD 

« Hide

References

[1]"Bifidobacterium asteroides PRL2011 Genome Analysis Reveals Clues for Colonization of the Insect Gut."
Bottacini F., Milani C., Turroni F., Sanchez B., Foroni E., Duranti S., Serafini F., Viappiani A., Strati F., Ferrarini A., Delledonne M., Henrissat B., Coutinho P., Fitzgerald G.F., Margolles A., van Sinderen D., Ventura M.
PLoS ONE 7:E44229-E44229(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PRL2011 EMBL AFU72251.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003325 Genomic DNA. Translation: AFU72251.1.
RefSeqYP_006865817.1. NC_018720.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFU72251; AFU72251; BAST_1684.
GeneID13809561.
KEGGbast:BAST_1684.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK4ISX5_BIFAP
AccessionPrimary (citable) accession number: K4ISX5
Entry history
Integrated into UniProtKB/TrEMBL: January 9, 2013
Last sequence update: January 9, 2013
Last modified: June 11, 2014
This is version 12 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)