ID   K3XX03_SETIT            Unreviewed;       436 AA.
AC   K3XX03;
DT   28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT   28-NOV-2012, sequence version 1.
DT   27-MAR-2024, entry version 70.
DE   RecName: Full=Alpha-amylase {ECO:0000256|ARBA:ARBA00012595, ECO:0000256|PIRNR:PIRNR001028};
DE            EC=3.2.1.1 {ECO:0000256|ARBA:ARBA00012595, ECO:0000256|PIRNR:PIRNR001028};
DE   AltName: Full=1,4-alpha-D-glucan glucanohydrolase {ECO:0000256|PIRNR:PIRNR001028};
GN   Name=101783371 {ECO:0000313|EnsemblPlants:KQL12203};
GN   ORFNames=SETIT_4G278200v2 {ECO:0000313|EMBL:RCV23178.1},
GN   SETIT_4G278300v2 {ECO:0000313|EMBL:RCV23179.1};
OS   Setaria italica (Foxtail millet) (Panicum italicum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC   Panicoideae; Panicodae; Paniceae; Cenchrinae; Setaria.
OX   NCBI_TaxID=4555 {ECO:0000313|EMBL:RCV23178.1};
RN   [1] {ECO:0000313|EMBL:RCV23178.1, ECO:0000313|Proteomes:UP000004995}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Yugu1 {ECO:0000313|EnsemblPlants:KQL12203,
RC   ECO:0000313|Proteomes:UP000004995}, and Yugu1
RC   {ECO:0000313|EMBL:RCV23178.1};
RX   PubMed=22580951; DOI=10.1038/nbt.2196;
RA   Bennetzen J.L., Schmutz J., Wang H., Percifield R., Hawkins J.,
RA   Pontaroli A.C., Estep M., Feng L., Vaughn J.N., Grimwood J., Jenkins J.,
RA   Barry K., Lindquist E., Hellsten U., Deshpande S., Wang X., Wu X.,
RA   Mitros T., Triplett J., Yang X., Ye C.Y., Mauro-Herrera M., Wang L., Li P.,
RA   Sharma M., Sharma R., Ronald P.C., Panaud O., Kellogg E.A., Brutnell T.P.,
RA   Doust A.N., Tuskan G.A., Rokhsar D., Devos K.M.;
RT   "Reference genome sequence of the model plant Setaria.";
RL   Nat. Biotechnol. 30:555-561(2012).
RN   [2] {ECO:0000313|EMBL:RCV23178.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=Yugu1 {ECO:0000313|EMBL:RCV23178.1};
RA   Noorani M.;
RL   Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
RN   [3] {ECO:0000313|EnsemblPlants:KQL12203}
RP   IDENTIFICATION.
RC   STRAIN=Yugu1 {ECO:0000313|EnsemblPlants:KQL12203};
RG   EnsemblPlants;
RL   Submitted (AUG-2018) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in
CC         polysaccharides containing three or more (1->4)-alpha-linked D-
CC         glucose units.; EC=3.2.1.1; Evidence={ECO:0000256|ARBA:ARBA00000548,
CC         ECO:0000256|PIRNR:PIRNR001028, ECO:0000256|RuleBase:RU361134};
CC   -!- COFACTOR:
CC       Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC         Evidence={ECO:0000256|ARBA:ARBA00001913,
CC         ECO:0000256|PIRNR:PIRNR001028};
CC   -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061, ECO:0000256|PIRNR:PIRNR001028,
CC       ECO:0000256|RuleBase:RU003615}.
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DR   EMBL; AGNK02002703; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CM003531; RCV23178.1; -; Genomic_DNA.
DR   EMBL; CM003531; RCV23179.1; -; Genomic_DNA.
DR   RefSeq; XP_004966428.1; XM_004966371.1.
DR   RefSeq; XP_004966429.1; XM_004966372.1.
DR   AlphaFoldDB; K3XX03; -.
DR   STRING; 4555.K3XX03; -.
DR   EnsemblPlants; KQL12203; KQL12203; SETIT_006461mg.
DR   EnsemblPlants; KQL12204; KQL12204; SETIT_008671mg.
DR   GeneID; 101782965; -.
DR   GeneID; 101783371; -.
DR   Gramene; KQL12203; KQL12203; SETIT_006461mg.
DR   Gramene; KQL12204; KQL12204; SETIT_008671mg.
DR   KEGG; sita:101782965; -.
DR   KEGG; sita:101783371; -.
DR   eggNOG; KOG0471; Eukaryota.
DR   HOGENOM; CLU_030069_1_0_1; -.
DR   InParanoid; K3XX03; -.
DR   OMA; LNPRTEI; -.
DR   OrthoDB; 2728918at2759; -.
DR   Proteomes; UP000004995; Chromosome IV.
DR   GO; GO:0004556; F:alpha-amylase activity; IBA:GO_Central.
DR   GO; GO:0005509; F:calcium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0005987; P:sucrose catabolic process; IBA:GO_Central.
DR   CDD; cd11314; AmyAc_arch_bac_plant_AmyA; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   InterPro; IPR012850; A-amylase_bs_C.
DR   InterPro; IPR013775; A-amylase_pln.
DR   InterPro; IPR006046; Alpha_amylase.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR43447; ALPHA-AMYLASE; 1.
DR   PANTHER; PTHR43447:SF38; ALPHA-AMYLASE ISOZYME 2A; 1.
DR   Pfam; PF07821; Alpha-amyl_C2; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   PIRSF; PIRSF001028; Alph-amls_plant; 1.
DR   PRINTS; PR00110; ALPHAAMYLASE.
DR   SMART; SM00642; Aamy; 1.
DR   SMART; SM00810; Alpha-amyl_C2; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Carbohydrate metabolism {ECO:0000256|RuleBase:RU361134};
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361134};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361134};
KW   Reference proteome {ECO:0000313|Proteomes:UP000004995};
KW   Signal {ECO:0000256|PIRNR:PIRNR001028}.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000256|PIRNR:PIRNR001028"
FT   CHAIN           24..436
FT                   /note="Alpha-amylase"
FT                   /evidence="ECO:0000256|PIRNR:PIRNR001028"
FT                   /id="PRO_5010604957"
FT   DOMAIN          24..366
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   DOMAIN          367..427
FT                   /note="Alpha-amylase C-terminal beta-sheet"
FT                   /evidence="ECO:0000259|SMART:SM00810"
FT   ACT_SITE        202
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001028-1"
FT   ACT_SITE        227
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001028-1"
SQ   SEQUENCE   436 AA;  47997 MW;  F3284ECA2D0BC407 CRC64;
     MGMGSRHMCC LSTLLLLLGL SSGQVLFQGF NWESSKQTGG WYNNLMGKVD DIAAAGVTHV
     WLPPPSHSVS SEGYMPGRLY DLDASKYGTA AELKSLIAAF HDKGVQAVAD IVINHRCADY
     KDSRGIYCIF EGGTPDSRLD WRAHMICRDD AKYSDGTGNL DTGADFPGAP DIDHLNDRVQ
     RELTEWMLWL KSDLGFDAWR LDFARGYSAE VARVYINGTA PSFVVAEIWD KMVPAGDDGK
     PAYDQDPHRQ ALVDWVDKVG GAASPATVFD FTTKGILNAA VEGELWRLID TEGKAPGVIG
     WWPAKAVTFV DNHDTGSTQA QWPFPHDKVM QGYAYILTHP GNPCIFYDHF FDWGFKDEIA
     ALVAVRKRNG ITPTSELTIL EHDGDAYVAE IDGKVIVKIG SRYDLGHLIP VGFEVATHGK
     DYAVWEKGGS EEVTRA
//