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Protein

Alkyl hydroperoxide reductase C

Gene

ahpC

Organism
Amphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides.1 Publication

Miscellaneous

The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this typical 2-Cys peroxiredoxin, C(R) is provided by the other dimeric subunit to form an intersubunit disulfide (PubMed:15770647). The disulfide is subsequently reduced by AhpF (By similarity).By similarity1 Publication

Catalytic activityi

2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.1 Publication

Kineticsi

  1. KM=8.9 µM for H2O21 Publication
  2. KM=10 µM for cumene hydroperoxide1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei48Cysteine sulfenic acid (-SOH) intermediate1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAntioxidant, Oxidoreductase, Peroxidase

    Protein family/group databases

    PeroxiBasei4918. AxAhpC.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkyl hydroperoxide reductase C (EC:1.11.1.151 Publication)
    Alternative name(s):
    Peroxiredoxin
    Thioredoxin peroxidase
    Gene namesi
    Name:ahpC
    Ordered Locus Names:AXY_22000
    OrganismiAmphibacillus xylanus (strain ATCC 51415 / DSM 6626 / JCM 7361 / LMG 17667 / NBRC 15112 / Ep01)
    Taxonomic identifieri698758 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeAmphibacillus
    Proteomesi
    • UP000006294 Componenti: Chromosome

    Subcellular locationi

    • Cytoplasm By similarity

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00004410692 – 188Alkyl hydroperoxide reductase CAdd BLAST187

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi48Interchain (with C-167); in linked formCombined sources1 Publication
    Disulfide bondi167Interchain (with C-48); in linked formCombined sources1 Publication

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    Induced significantly under aerobic conditions.1 Publication

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked, upon oxidation (PubMed:10960086, PubMed:15770647). 5 homodimers assemble to form a ring-like decamer (PubMed:15770647).2 Publications

    Structurei

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1WE0X-ray2.90A/B/C/D/E/F/G/H/I/J2-188[»]
    ProteinModelPortaliK0J4Q8.
    SMRiK0J4Q8.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO87200.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini2 – 158ThioredoxinPROSITE-ProRule annotationAdd BLAST157

    Sequence similaritiesi

    Keywords - Domaini

    Redox-active center

    Phylogenomic databases

    KOiK03386.
    OMAiCPANWEE.
    OrthoDBiPOG091H026G.

    Family and domain databases

    InterProiView protein in InterPro
    IPR017559. AhpC.
    IPR000866. AhpC/TSA.
    IPR024706. Peroxiredoxin_AhpC-typ.
    IPR019479. Peroxiredoxin_C.
    IPR012336. Thioredoxin-like_fold.
    IPR013766. Thioredoxin_domain.
    PANTHERiPTHR10681:SF128. PTHR10681:SF128. 1 hit.
    PfamiView protein in Pfam
    PF10417. 1-cysPrx_C. 1 hit.
    PF00578. AhpC-TSA. 1 hit.
    PIRSFiPIRSF000239. AHPC. 1 hit.
    SUPFAMiSSF52833. SSF52833. 1 hit.
    TIGRFAMsiTIGR03137. AhpC. 1 hit.
    PROSITEiView protein in PROSITE
    PS51352. THIOREDOXIN_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    K0J4Q8-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSLIGTEVQP FRAQAFQSGK DFFEVTEADL KGKWSIVVFY PADFSFVCPT
    60 70 80 90 100
    ELEDVQKEYA ELKKLGVEVY SVSTDTHFVH KAWHENSPAV GSIEYIMIGD
    110 120 130 140 150
    PSQTISRQFD VLNEETGLAD RGTFIIDPDG VIQAIEINAD GIGRDASTLI
    160 170 180
    NKVKAAQYVR ENPGEVCPAK WEEGGETLKP SLDIVGKI
    Length:188
    Mass (Da):20,774
    Last modified:November 28, 2012 - v1
    Checksum:iE61BA53C0215BA84
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB018435 Genomic DNA. Translation: BAA33808.1.
    AP012050 Genomic DNA. Translation: BAM48332.1.
    RefSeqiWP_015010915.1. NC_018704.1.

    Genome annotation databases

    EnsemblBacteriaiBAM48332; BAM48332; AXY_22000.
    KEGGiaxl:AXY_22000.
    PATRICifig|698758.3.peg.2211.

    Similar proteinsi

    Entry informationi

    Entry nameiAHPC_AMPXN
    AccessioniPrimary (citable) accession number: K0J4Q8
    Secondary accession number(s): O87200
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2017
    Last sequence update: November 28, 2012
    Last modified: August 30, 2017
    This is version 26 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families