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K0F800 (K0F800_9NOCA) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 5. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable DNA ligase HAMAP-Rule MF_00407
EC=6.5.1.1 HAMAP-Rule MF_00407
Alternative name(s):
Polydeoxyribonucleotide synthase [ATP] HAMAP-Rule MF_00407
Gene names
Name:ligB EMBL AFU05832.1
Synonyms:lig HAMAP-Rule MF_00407
ORF Names:O3I_039425 EMBL AFU05832.1
OrganismNocardia brasiliensis ATCC 700358 EMBL AFU05832.1
Taxonomic identifier1133849 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Nocardiaceae › Nocardia ›

Protein attributes

Sequence length503 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP-Rule MF_00407

Catalytic activity

ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). RuleBase RU000617 HAMAP-Rule MF_00407

Cofactor

Divalent metal cations By similarity. HAMAP-Rule MF_00407

Sequence similarities

Belongs to the ATP-dependent DNA ligase family. RuleBase RU004196 HAMAP-Rule MF_00407

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Active site2121N6-AMP-lysine intermediate By similarity HAMAP-Rule MF_00407
Binding site2101ATP By similarity HAMAP-Rule MF_00407
Binding site2171ATP By similarity HAMAP-Rule MF_00407
Binding site2321ATP By similarity HAMAP-Rule MF_00407
Binding site2611ATP By similarity HAMAP-Rule MF_00407
Binding site2971ATP By similarity HAMAP-Rule MF_00407
Binding site3681ATP By similarity HAMAP-Rule MF_00407
Binding site3741ATP By similarity HAMAP-Rule MF_00407

Sequences

Sequence LengthMass (Da)Tools
K0F800 [UniParc].

Last modified November 28, 2012. Version 1.
Checksum: C8B41995FFB5D5D3

FASTA50354,337
        10         20         30         40         50         60 
MLFSHVVQAS ETVRATRSRK TKIAAFAELL TAAAPDELAP VVAWVSGELR QGRIGTGWRT 

        70         80         90        100        110        120 
LAGLDTPPAP VATLTVSAVD TALSEVAETS GTGSTARRKE LLVALWTAAT AEEQAFLLRL 

       130        140        150        160        170        180 
LTGELRQGAL AAIVSEAVAV AAAVPVELVR RAYMLSGRLP VTALAALTGG VAALREFRLE 

       190        200        210        220        230        240 
VGRPIQPMLA APGASLDEAL TEFDGKVSAE IKMDGARIQV HRTGPRVWVF TRTLRDITAG 

       250        260        270        280        290        300 
VPELVDLVRG LDCDSVVLDG ETLALDDSGR PRPFQETMSR FAEVSSTREL LLHPYFFDCL 

       310        320        330        340        350        360 
HLDGQDLLDA PLSERRAALT KVAGEHSIPA LLEPDAEAAA EYFDGALAAG HEGVMIKSLA 

       370        380        390        400        410        420 
APYAAGRRGR AWQKIKPTHT LDLLVLGAEW GYGRRTGYLS NLHLGARDPL TGEPVMVGKT 

       430        440        450        460        470        480 
FKGLTDALLQ WQTAEFPRHE RTRDEHTVYL YPELVVEIAL DGVQVSPRYP GGVALRFARV 

       490        500 
VRYRPDKEAR DADTIDTVRA LLP

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References

[1]"Complete genome sequence of Nocardia brasiliensis HUJEG-1."
Vera-Cabrera L., Ortiz-Lopez R., Elizondo-Gonzalez R., Perez-Maya A.A., Ocampo-Candiani J.
J. Bacteriol. 194:2761-2762(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: HUJEG-1 EMBL AFU05832.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP003876 Genomic DNA. Translation: AFU05832.1.
RefSeqYP_006812806.1. NC_018681.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAFU05832; AFU05832; O3I_039425.
GeneID13731643.
KEGGnbr:O3I_039425.

Phylogenomic databases

KOK01971.

Family and domain databases

Gene3D1.10.3260.10. 1 hit.
2.40.50.140. 1 hit.
HAMAPMF_00407. DNA_ligase.
InterProIPR022865. DNA_ligae_ATP-dep_bac/arc.
IPR000977. DNA_ligase_ATP-dep.
IPR012309. DNA_ligase_ATP-dep_C.
IPR012310. DNA_ligase_ATP-dep_cent.
IPR016059. DNA_ligase_ATP-dep_CS.
IPR012308. DNA_ligase_ATP-dep_N.
IPR012340. NA-bd_OB-fold.
[Graphical view]
PfamPF04679. DNA_ligase_A_C. 1 hit.
PF01068. DNA_ligase_A_M. 1 hit.
PF04675. DNA_ligase_A_N. 2 hits.
[Graphical view]
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF117018. SSF117018. 1 hit.
TIGRFAMsTIGR00574. dnl1. 1 hit.
PROSITEPS00697. DNA_LIGASE_A1. 1 hit.
PS50160. DNA_LIGASE_A3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameK0F800_9NOCA
AccessionPrimary (citable) accession number: K0F800
Entry history
Integrated into UniProtKB/TrEMBL: November 28, 2012
Last sequence update: November 28, 2012
Last modified: April 3, 2013
This is version 5 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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